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Effectiveness Meniran ( Phyllanthus Niruri Linn ) as Immunomodulation onLymphocyte of Broiler Infected with Enterotoxin Escherichia Coli Resistant Antibiotics Wahjuni, Retno Sri; Sabdoningrum, Emy Koestanti; Hidanah, Sri
Jurnal Vokasi Indonesia Vol. 5, No. 2
Publisher : UI Scholars Hub

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Abstract

Enterotoxin Escherichia coli resistant to antibiotics on broiler. The used of antibiotics should be re- evaluated, as well as over Escherichia coli enterotoxin. Plants meniran (Phyllanthus niruri Linn) is a plant that can be used as an alternative prevention and treatment of diseases caused by Escherichia coli enterotoxin. In this research, broilers at the age of 23 days were divided into six groups. Two groups are positive control was infected by Escherichia coli and negative control was not infected by Escherichia coli. Three groups are extract of Phyllanthus niruri Linn. plant that variety in three dosage: 20%, 25%, 30% and antibiotic. After five days treatment the broiler was to take the sample of blood tissue. The data were tested with Annova and continued by Duncan multiple range test. There was a significant difference of immunomodulation activity between treatment group doses 30% has been able to decrease lymphocyte on blood. The result showed doses 30% can be used as immunomodulation to Escherichia coli.
EFFECTIVENESS OF MENIRAN (PHYLLANTHUS NIRURI LINN) AS ANTIBACTERIAL FOR RESISTANCE ANTIBIOTICS PREVENTION OF ENTEROTOXIN ESCHERICHIA COLI Hidanah, Sri; Sabdoningrum, Emy Koestanti; Wahyuni, Retno Sri; Dewi, Arini Rahmi; Safitri, Erma -
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.153 KB) | DOI: 10.20473/ijtid.v7i2.7328

Abstract

Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which showed 21% resistance to various antibiotics, such as enterotoxigenic E.coli. Alternative efforts are needed to overcome this, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The aim of research is the research was done through several stages: isolation and identification of enterotoxigenic E. coli from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against enterotoxigenic E. coli n The study was divided into five treatments: T0+ (group of chickens were infected by enterotoxigenic E. coli T0- (control group, not infected), T1 (infected by enteroxigenic E. coli + 20% meniran extract), T2 (infected by enterotoxigenic E. coli + 25% extract meniran), T3 (infected by enterotoxigenic E. coli + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results show that all of E. coli DNA isolates which tested by the PCR method show positive reactions at 600 bp. The next stage, the enterotoxigenic E. coli are resistant to some antibiotics, such as amoxicillin, ampicillin, erythromycin, cephalosporins, tetracycline, cloxacillin and gentamicin. Furthermore, the 30% Phyllanthus niruri Linn extract is effective as an antibacterial for antibiotic resistance enterotoxigenic E. coli. next necessary to write that: The 30% meniran extract is potential for kill of enterotoxigenic E. coli
Efektifitas Probiotik Lactobacillus casei dan Lactobacillus rhamnosus Sebagai Pengganti Antibiotic Growth Promoter Terhadap Total Kolesterol, Low Density Lipoprotein dan High Density Lipoprotein Ayam Broiler Andriani, Anita Dwi; Lokapirnasari, Widya Paramita; Karimah, Balqis; Hidanah, Sri; Al-Arif, M. Anam; Soeharsono, Soeharsono; Harijani, Nenny
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.324 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.114-122

Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas probiotik Lactobacillus casei dan Lactobacillus rhamnosus sebagai pengganti Antibiotic Growth Promoter (AGP) terhadap total kolesterol, Low Density Lipoprotein (LDL) dan High Density Lipoprotein (HDL) pada ayam pedaging. Ayam broiler sebanyak 18 ekor day old chick diacak kedalam enam perlakuan (P0, P1, P2, P3, P4 dan P5) setiap perlakuan terdiri dari 3 ulangan, yakni (P0) 100% pakan basal, (P1) pakan basal+0.01 gram AGP/kg pakan, (P2) pakan basal+probiotik 0.05 gram/kg pakan, (P3) pakan basal+probiotik 0.1 gram/kg pakan, (P4) pakan basal+probiotik 0.025 gram/liter air minum, (P5) pakan basal+probiotik 0.05 gram/liter air minum. Data di analisis menggunakan uji Multivariate Analysis of Variance (MANOVA) dilanjutkan uji Duncan. Hasil menunjukkan bahwa pemberian probiotik berbeda nyata (p<0.05) dengan pemberian AGP. Pemberian probiotik L. casei dan L. rhamnosus dengan dosis 0.05 gram/kg pakan, 0.01 gram/kg pakan, 0.025 gram/liter air minum dan 0.05 gram/liter air minum sebagai pengganti AGP dapat menurunkan total Kolesterol dan LDL serta meningkatkan kadar HDL pada ayam broiler. Dapat disimpulkan bahwa penambahan probiotik pada pakan dan air minum dapat menurunkan total kolesterol dan LDL serta menaikkan kadar HDL pada ayam broiler.
Produksi dan Kualitas Telur Itik Alabio di Daerah Sentra Peternakan Desa Sungai Pandan, Kabupaten Hulu Sungai Utara, Kalimantan Selatan Fajarwati, Rini; Sarmanu, Sarmanu; Nidom, Chairul Anwar; Madyawati, Sri Pantja; Mustofa, Imam; Lamid, Mirni; Hidanah, Sri; Lokapirnasari, Widya Paramita; Purnomo, Tarzan; Sukmanadi, Mohammad
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.246-250

Abstract

Penelitian ini merupakan penelitian deskriptif dengan menggunakan 20 itik Alabio betina siap bertelur dengan rentang usia 6 bulan dan untuk pemeriksaan kualitas telur untuk masing-masing kelompok 10 sampel. Pengamatan tingkat produksi telur dilakukan selama tiga bulan dengan 10 pengulangan, kualitas telur (ketebalan kulit telur, persentase kulit telur, persentase kulit telur, persentase albumin, persentase kuning telur, huagh unit, warna kuning telur, kadar protein dan lemak kandungan). Data produksi telur dan kualitas telur diuji menggunakan uji Anova. Hasil pengamatan rata-rata produksi harian telur 15.45 ± 0.12, 14.72 ± 0.10, 14.91 ± 0.09; produksi berat harian (kg/hari) 925.27 ± 7.50, 875.40 ± 6.32, 884 ± 5.03; hasil dari ketebalan kulit telur kualitas 0.33 ± 0.01, 0.33 ± 0.12, 0.33 ± 0.12; persentase kulit telur 11.32 ± 0.93, 11.28 ± 0.10, 11.36 ± 0.05; persentase albumin 55.70 ± 1.08, 56.66 ± 1.22, 56.12 ± 1.00; persen kuning telur 32.07 ± 1.24, 31.55 ± 1.48, 31.64 ± 1.20; Haugh unit 88.70 ± 6.21, 91.41 ± 6.70, 94.51 ± 5.06. Warna kuning masing-masing memiliki skor 15. Dapat disimpulkan bahwa produksi dan kualitas telur itik Alabio baik.
Potensi Pemberian Probiotik Bifidobacterium sp., Lactococcus lactis, dan Lactobacillus sp. Terhadap Performa Produksi Ayam Petelur Irawan, Putri Indra; Lokapirnasari, Widya Paramita; Al Arif, M. Anam; Harijani, Nenny; Soeharsono, Soeharsono; Hidanah, Sri
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.160-165

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian probiotik Bifidobacterium sp., Lactococcus lactis, dan Lactobacillus sp. terhadap efisiensi pakan dan massa telur ayam Isa Brown. Penelitian ini menggunakan ayam petelur strain Isa Brown sebanyak 36 ekor umur 30 minggu. Konsentrasi probiotik yang digunakan yaitu 1.2x109 CFU/ml. Tiga perlakuan dalam penelitian yaitu P0 (tanpa diberi probiotik), P1 (diberi probiotik sebanyak 1 ml/liter air minum), dan P2 (diberi probiotik sebanyak 2 ml/liter air minum). Hasil penelitian menunjukkan bahwa nilai massa telur pada P2 berbeda nyata (p<0.05) dengan P1 dan P0, namun P1 tidak berbeda nyata (p>0.05) dengan P0. Berdasarkan hasil efisiensi pakan, probiotik dapat memberikan perbedaan yang nyata (p<0.05) antar perlakuan baik P0, P1, maupun P2. Probiotik sebanyak 2 ml/liter air minum (P2) menunjukkan nilai efisiensi pakan tertinggi (73.88 ± 4.06), sedangkan nilai efisiensi pakan terendah terjadi pada perlakuan P0. Kesimpulan penelitian ini adalah pemberian probiotik Bifidobacterium sp., L. lactis, dan Lactobacillus sp. dosis 1 ml/liter air minum maupun dosis 2 ml/liter air minum dapat meningkatkan efisiensi pakan namun tidak pada massa telur ayam Isa Brown.
Effect of Probiotics Bacillus subtilis on Feed Efficiency and Egg Mass of Laying Hens Pratama, Hanif Sabekti; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono; Al-Arif, Muhammad Anam; Harijani, Nenny; Hidanah, Sri
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.37-41

Abstract

The aim of this research was to investigate the effect of probiotics Bacillus subtilis on feed efficiency and egg mass of laying hens. This research was using 36 of 38 weeks-old laying hens Isa Brown strain. The concentration of probiotics B. subtilis was 1 x 109 CFU/ml, there were three kinds of treatments, P0 (without given probiotics B. subtilis), P1 (given 0.1 gram of probiotics B. subtilis per kg of feed), and P2 (given 0.2 gram of probiotic B. subtilis per kg of feed). The results of feed efficiency, the provision of probiotics can provide a significantly different values (p <0.05) between treatments either P0, P1, or P2. Provision probiotics P1 showed the highest value of feed efficiency (81.72 ± 5.28), while the lowest value at P0 was (64.06 ± 3.30). The results showed that there were significantly different in egg mass values (p <0.05) between treatments both P0, P1, and P2. Probiotic P2 shows the highest value (53.30 ± 2.13), while the lowest egg mass value of probiotics is as much as P1 (40.83 ± 4.73). Based on the results of this study it can be concluded that the B. subtilis probiotics both at a dose of 0.1 gram / kg of feed and a dose of 0.2 gram / kg of feed can affect the growth performance of laying hens.
Administration of Palm Kernel Meal and íŸ-Mannanase on Laying Hens Productivity Wicaksana, Dimas; Hidanah, Sri; Lokapirnasari, Widya Paramita; Al-Arif, Muhammad Anam; Lamid, Mirni; Suprianondo, Koesnoto
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.72-77

Abstract

The aim of this research was to observe the influence of palm kernel meal and íŸ-Mannanase on laying hens. Laying hens up to 2500 birds were reared in opened-house cage fed with commercial feed substituted with palm kernel meal and íŸ-Mannanase. All hens were divided into five groups in an order P0 as control group, groups without enzyme which are P1 with 7% palm kernel meal and P2 with 14% palm kernel meal, groups with íŸ-Mannanase which are P3 7% palm kernel meal and P4 with 14% palm kernel meal. The observed variables are feed intake, feed cost, egg weight, egg mass, feed conversion ratio, hen day production, and mortality rate. The results indicated that threr are no significant difference from all variables. The percentage of palm kernel meal for 14% with íŸ-Mannanase cannot give any influence on laying hens productivity.
Supplementation of Fermented Moringa Leaf Powder (Moringa oleifera) on Feed Consumption, Egg Weight and Feed Conversion Ratio (FCR) in Laying Duck Pramestya, Ninda Rubi; Hidanah, Sri; Lamid, Mirni; Soepranianondo, Koesnoto; Lokapirnasari, Widya Paramita; Al-Arif, Mohammad Anam; Soeharsono, Soeharsono
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.78-83

Abstract

This study aimed to determine the effect of the addition of fermented moringa leaf powder on feed consumption, egg weight, Feed Conversion Ratio (FCR) of laying ducks. 60 laying ducks were randomized into four treatments i.e. (P0) 0% Moringa leaf powder fermentation, (P1) 0.5% Moringa leaf powder fermentation, (P2) 1% Moringa leaf powder fermentation, and (P3) 1.5% Moringa leaf fermentation. Treatment of concentration refered in 100% commercial feed for 3 weeks. The results showed that the adding of Moringa leaf powder fermentation to feed consumption in each treatment showed significantly different results (p <0.05) in each treatment, with the highest consumption at P2 (176.21 ± 0.92) and the lowest at P0 (158.21 ± 1.07). The results of the study on duck egg weight showed that P1, P2, P3 were significantly different with P0. The highest egg weight in P2 is (70.50 ± 0.34) and the lowest is P0 (66.98a ± 0.29). FCR of laying duck in each treatment showed significantly different results in each treatment, with the highest at P0 (3,81± 0,34) and the lowest at P2 (3,27 ± 0,09). In conclusion, the addition of 1% Moringa leaf powder in 100% of commercial feed can increase feed consumption, increase egg weight and decrease FCR.
Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat Plumeriastuti, Hani; Weliyani, Trima; Hamid, Iwan Sahrial; Hidanah, Sri; Prastiya, Ragil Angga; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.243-248

Abstract

Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.
The Effect of Additional Feed Fermentation of Moringa Oleifera Leaves on The Cholesterol Level of Mojosari Laying Ducks Lestari, Ratna Dwi; Lokapirnasari, Widya Paramita; Al Arif, Muhammad Anam; Hidanah, Sri; Soeharsono, Soeharsono; Lamid, Mirni
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.221-225

Abstract

The purpose of this research was to determine the effect of supplementary feeding with fermented Moringa leaf flour on cholesterol levels of Mojosari duck eggs and to calculate the cost of production. This study used 60 mojosari ducks, layer phase aged 27 weeks. Moringa flour was fermented using probiotics and given in four treatments, namely P0 (100% commercial feed), P1 (0.5% moringa leaf flour fermentation + 100% commercial feed), P2 (1% moringa leaf flour fermentation + 100% commercial feed), P3 (1.5% fermented Moringa leaf meal + 100% commercial feed). The variable observed in this study was cholesterol levels in Mojosari duck eggs. The results showed thatgiving Moringa leaf flour fermentation can give significantly different values (p <0.05) in each treatment both P0, P1, P2 and P3.In the fermentation of moringa leaf flour as much as 1.5% (P3) had the lowest cholesterol levels (113.13 ± 12.55) Meanwhile, 1% (P2) of moringa leaf flour fermentation has the highest cholesterol level (170.42 ± 13.19).
Co-Authors Adikara, Tatang Santanu Al Arif, M. Anam Al Arif, Moh. Anam Al arif, Mohammad Anam Al Arif, Muhammad Anam Al-Arif , M. Anam Al-Arif, M. Anam Al-Arif, Muhammad Anam Andriani, Anita Dwi Andriani, Asafarid Benjamin Christoffel Tehupuring, Benjamin Christoffel Berlina, Cyrcilia Relita Bugiono, Bugiono Caska - Chandra, Evania Haris Chusniati, Sri Dewi, Arini Rahmi Erma Safitri Fajarwati, Rini Fatin Fadhilah Hasib Firdaus, Muhammad Aviv Fuddin, Muhammad Nazar Hadiah Fitriyah Hani Plumeriastuti Hapsari, Tiara Harijani, Nenny Hasan, Denny Irmawati Hendarti, Gracia Angelina Hernanda, Ary Setya Herry Agoes Hermadi Hidayatik, Nanik IMAM MUSTOFA Ira Sari Yudaniayanti Irawan, Adinda Riezki Irawan, Putri Indra Ishaq, Rizal Maulana Iwan Sahrial Hamid Kadek Rachmawati Karimah, Balqis Khairullah, Aswin Rafif Kirana, Amelia Lintang Putri Koestanti S, Emy Kusnoto Kusnoto, Kusnoto Larasati, Anissa Dhea Lestari, Ratna Dwi Lilik Maslachah Lisnanti, Ertika Fitri Lovela, Aprinda Ratna Luqman, Epy Muhammad Ma'ruf, Muchammad Achsinul Fikri Miarsono, Sigit Mirni Lamid Mufasirin Nayan, Nazri Nidom, Chairul Anwar Nurliyani Nurliyani Prakoso, Febriyono Anggela Pramestya, Ninda Rubi Prasinta, Redilla Prastiya, Ragil Angga Pratama, Hanif Sabekti Rachmat, Rd.Lutpi Raharjo, Dadik Rahmawati, Kadek Ritonga, Mudhita Zikkrullah Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanti Safitri, Erma - Sarmanu, Sarmanu Setyawan, Boedi Silfia, Himatul Ilma Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Pantja Madyawati, Sri Pantja Suharsono Suharsono Suherni Susilowati Sukmanadi, Mohammad Suprianondo, Koesnoto Suprihati , Endang TARZAN PURNOMO Tintin Sukartini Sukartini, Tintin Sukartini Utomo, Gogik Satrio Margo Wahjuni, Retno Sri Wahyuni, Retno Sri Warsito, Sunaryo Hadi Weliyani, Trima Wibawati, Prima Ayu Wicaksana, Dimas Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Yeni Dhamayanti Yudanianyi, Ira Sari Yulianna Puspitasari ZULFAH