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All Journal Jurnal Kedokteran Brawijaya Biomedical Journal of Indonesia
Saleh, M Irsan
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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IFN-GAMMA SECRETION AND IL-10 AFTER STIMULATION OF ESAT-6-CFP-10 (EC610) FUSION ANTIGEN IN PATIENTS WITH ACTIVE TUBERCULOSIS AND LATENT TUBERCULOSIS Prihantika S., Sabrina; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, M Irsan; Hafy, Zen; Tanoerahardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 5, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i3.8897

Abstract

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis which is still a health problem in the world including in Indonesia. TB infection arises due to interference with the regulation of the body's defense system. Cellular immune system plays a role in fighting TB infection, namely the role of T lymphocytes that differentiate into Th1 cells secrete pro-inflammatory cytokines IFN-? and Th2 cells that secrete IL-10 anti-inflammatory cytokines. The EC610 fusion antigen is specific and has a strong antigenicity to T cell stimulation. The aim of the study was to determine differences in the mean levels of IFN-? and IL-10 after EC610 antigen stimulation in patients with active TB and latent TB. The design of this study was a quasi-in vitro experiment with PBMC culture stimulated by EC610 antigens in the active TB and latent TB groups. The research and examination were carried out at the South Sumatra Province SSR and the Health Research and Development Laboratory Center, Central Jakarta. There were 21 subjects with active TB and 28 subjects with latent TB who met the inclusion criteria. Measurement of IFN-levels? and IL-10 was performed using ELISA-Reader. The results showed the levels of IFN-? and IL-10 after EC610 antigen stimulation were higher in active TB than latent TB. High levels of IFN-? active TB patients showed protective immune responses against M.tb germs while high levels of IL-10 showed their role as anti-inflammatory. There is no significant difference in the average level of IFN-? in patients with active TB and latent TB (p = 0.769) and there was a significant difference in the level of IL-10 levels after EC610 antigen stimulation was higher in active TB than latent TB (p = 0,000).
The Effect of Centella asiatica Cream on Transforming Growth Factor Beta 1 in Acute Wounds Ulfa, Maria; Iskandar, Elza; Erna, Riani; Saleh, M Irsan
Jurnal Kedokteran Brawijaya Vol. 34 No. 1 (2026)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2026.034.01.6

Abstract

Centella asiatica contains triterpenoids with anti-inflammatory, antioxidant, and antifibrotic properties and has long been used in wound healing. Previous studies suggest that Centella asiatica may reduce TGF-β1 expression, which is implicated in scar and keloid formation. Given the pleiotropic role of TGF-β in wound healing being required in the inflammatory phase and downregulated during proliferation this study employed an acute wound model to represent normal healing and to evaluate the effect of Centella asiatica extract on TGF-β1 expression. This study aimed to evaluate the effects of topical Centella asiatica cream application at concentrations of 0.5%, 1%, and 2% on TGF-β1 levels in acute wound healing in Wistar rats. An experimental posttest-only control group design was conducted at the Pharmacology Laboratory, Animal House, and Biotechnology Laboratory, Faculty of Medicine, Universitas Sriwijaya. Five groups were studied, consisting of one untreated group, three groups treated with Centella asiatica cream at concentrations of 0.5%, 1%, and 2%, respectively, and one group treated with placebo, with five rats in each group. Data were analyzed using  One-way ANOVA, and Post Hoc LSD tests. Results showed the highest TGF-β1 levels in the placebo group and the lowest in the 1% Centella asiatica cream group. Significant differences were found between the 1% Centella asiatica cream group and the untreated  (p=0.026), placebo, and 2% Centella asiatica cream groups (p=0.008).  In conclusion, the Centella asiatica cream concentration of 1% was the most effective in reducing TGF-β1 levels in acute eyelid wound healing in Wistar rats.
Co-Authors Eddy Mart Salim, Eddy Mart Elza Iskandar, Elza Jusak Nugraha kurniati, nova Maria Ulfa Prihantika S., Sabrina Rahadiyanto, Kemas Ya'Kub Riani Erna, Riani Tanoerahardjo, Francisca Srioetami Zen Hafy