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IFN-GAMMA AND IL-4 SECRETION AFTER STIMULATION OF EC610 FUSION ANTIGENS (ESAT-6-CFP-10) IN PATIENTS WITH ACTIVE PULMONARY TB AND LATENT TB Safutri, Wina; Kurniati, Nova; Tanoehardjo, Fransisca Srioetami; Rahadiyanto, Kemas Yakub
Biomedical Journal of Indonesia Vol 6, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i1.9087

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis (M.tb) and is still a major health problem in the world. Immunity against tuberculosis is very complex because it involves almost all components of the immune system. One of the cells that are responsible for cell-mediated immunity are lymphocytes, especially T helper lymphocytes are divided into Th1 (proinflammatory cytokines IFN-?) and Th2 (anti-inflammatory cytokine IL-4). EC610 fusion antigens (ESAT-6-CFP-10) is a peptide containing a specific antigen M.tb. Order to determine the differences between the secretion of IFN-? and IL-4 after stimulation EC610 fusion antigens (ESAT-6-CFP-10) in patients with active pulmonary TB and latent TB.Research with a quasi-experimental design in laboratory in vitro in patients with active pulmonary tuberculosis group of 21 samples and 28 samples of latent TB. Venous blood sampling in vitro anticoagulants heparin and PBMCs isolated using a Ficoll-Paque ?, then cultured with antigen fusion EC610 for 24-72 hours with a CO2 incubator at a temperature of 370 C. The levels of IFN-? and IL-4 by ELISA method. Statistical analysis with alternative test non-parametric Mann Whitney.IFN-? secretion in patients with active pulmonary TB group (6700 pg / ml) was higher than the latent (6000 pg / ml) after stimulation by antigen fusion EC610, but not significant (p = 0.769). While the secretion of IL-4 levels (180 and 60 pg / ml) there was a significant difference between the groups (p = 0.000).

IFN-GAMMA SECRETION AND IL-10 AFTER STIMULATION OF ESAT-6-CFP-10 (EC610) FUSION ANTIGEN IN PATIENTS WITH ACTIVE TUBERCULOSIS AND LATENT TUBERCULOSIS Prihantika S., Sabrina; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, M Irsan; Hafy, Zen; Tanoerahardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 5, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i3.8897

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis which is still a health problem in the world including in Indonesia. TB infection arises due to interference with the regulation of the body's defense system. Cellular immune system plays a role in fighting TB infection, namely the role of T lymphocytes that differentiate into Th1 cells secrete pro-inflammatory cytokines IFN-? and Th2 cells that secrete IL-10 anti-inflammatory cytokines. The EC610 fusion antigen is specific and has a strong antigenicity to T cell stimulation. The aim of the study was to determine differences in the mean levels of IFN-? and IL-10 after EC610 antigen stimulation in patients with active TB and latent TB. The design of this study was a quasi-in vitro experiment with PBMC culture stimulated by EC610 antigens in the active TB and latent TB groups. The research and examination were carried out at the South Sumatra Province SSR and the Health Research and Development Laboratory Center, Central Jakarta. There were 21 subjects with active TB and 28 subjects with latent TB who met the inclusion criteria. Measurement of IFN-levels? and IL-10 was performed using ELISA-Reader. The results showed the levels of IFN-? and IL-10 after EC610 antigen stimulation were higher in active TB than latent TB. High levels of IFN-? active TB patients showed protective immune responses against M.tb germs while high levels of IL-10 showed their role as anti-inflammatory. There is no significant difference in the average level of IFN-? in patients with active TB and latent TB (p = 0.769) and there was a significant difference in the level of IL-10 levels after EC610 antigen stimulation was higher in active TB than latent TB (p = 0,000).

IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.

IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.

Faktor risiko dan gejala osteoporosis pada komunitas dewasa di Desa Ibul Besar Pemulutan Ogan Ilir Sumatera Selatan Umi Partan, Radiyati; Reagan, Muhammad; Hermansyah, Hermansyah; Darma, Surya; Muthia, Putri; Mediarty, Mediarty; Indrajaya, Taufik; Kurniati, Nova; Riviati, Nur; Yuniza, Yuniza
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 2 No 3 (2021): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V2I3.68

. Osteoporosis is a worldwide health issue. The microarchitecture of the bone is deteriorating, which increases the risk of fracture and disability. As the Indonesian people's life expectancy increases, the incidence of osteoporosis rises, making it more difficult to manage. This community service is carried out as a form of preventive treatment in the adult community in the village of Ibul Besar III Pemulutan Ogan Ilir, South Sumatra, which is one of Sriwijaya University's guided villages, with counseling methods to increase knowledge and health checks for early detection of osteoporosis, as well as calcium supplementation. There were 81 participants in this activity, with numerous dominant risk factors for osteoporosis identified, including female gender, menopausal state, and age above 50. Musculoskeletal symptoms, such as pain in the waist and back, which can be a consequence of osteoporosis, are more common than non-musculoskeletal symptoms.

Manifestasi klinis dan derajat aktivitas penyakit pada penyandang Lupus Eritematosus Sitemik di Rumah Sakit Dr. Mohammad Hoesin Palembang Partan, Radiyati Umi; Hermansyah, Hermansyah; Darma, Surya; Reagan, Muhammad; Muthia, Putri; Salim, Eddy Mart; Kriswiastiny, Rina; Kurniati, Nova; Yuniza, Yuniza; Silitonga, Aira Priamas
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 5 No 1 (2024): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V5I1.110

Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease that can affect various organs. Clinical manifestations vary from mild to severe depending on the organs involved. Manifestations of SLE in the kidney known as lupus nephritis can occur in 60% of the SLE population and are more common in Asian, Hispanic, Native American, and Black populations, especially in women of childbearing age, involvement of other organs such as neurology is referred to as neuropsychiatric lupus, as well as other organs can manifest such as mucocutaneous lupus, arthritis, pericarditis and other organs. This community service is carried out as a form of preventive treatment with counseling methods to increase knowledge and health checks for early detection of SLE, as well as promotive efforts for the SLE community in the disease polyclinic at Dr. Mohammad Hoesin Palembang, South Sumatra which is one of the teaching hospitals of the Faculty of Medicine, Sriwijaya University. This activity was attended by 35 people with SLE with several risk facts such as female gender and middle adulthood which is quite common, and some of the systemic clinical manifestations experienced are almost fatigue and fever, for musculoskeletal manifestations the most commonly experienced is arthritis, while the most common manifestations of other organs are mucocutaneous disorders. Keywords: Lupus, Autoimmune, Disease Activity

Association Between LENT Score and Survival in Patients with Malignant Pleural Effusion at Dr. Mohammad Hoesin General Hospital, Palembang Rizkyani Sariza, Fania; Ahmad, Zen; Kurniati, Nova; Yusri, Muhammad; Irfannudin; Sudarto; Andriani, Raden Ayu Linda; Pasaribu, Rouly Pola; Rachman, Alif Fathur
Sriwijaya Journal of Medicine Vol. 8 No. 2 (2025): Vol 8, No 2, 2025
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v8i2.352

The presence of a malignant pleural effusion (MPE) indicates advanced stage of malignancy, which severely compromises in a poor prognosis. LENT score has been developed as a prognostic tool for MPE, yet its association with survival has not been thoroughly evaluated in Indonesia. This study aims to examine the association between LENT score and 3-month survival in patients with MPE at Dr. Mohammad Hoesin General Hospital, Palembang. This prospective cohort study included 35 patients with MPE. Survival analysis using Kaplan-Meier curves. In this study cohort, the most frequent primary malignancy observed was lung cancer (62,8%). Kaplan-Meier analysis showed a median survival of 22 days for the high-risk LENT scores and 85 days for the moderate-risk group (p < 0,001). LENT score is significantly associated with three-month mortality in EPG patients. LENT score remains a robust tool for risk stratification in MPE management.

Correlation of TNF-α with Systemic Lupus Erythematosus Activity in Mohammad Hoesin Hospital Palembang Ismed, Yonis; Kurniati, Nova; Theodorus; Yuniza; Salim, Eddy Mart
Sriwijaya Journal of Medicine Vol. 8 No. 3 (2025): Vol 8, No 3, 2025 (Issue In Progress)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v8i3.349

Systemic lupus erythematosus (SLE) is a persistent autoimmune disorder marked by immunological dysregulation and widespread inflammation. Tumor necrosis factor-α (TNF-α), a pivotal pro-inflammatory cytokine, has been associated with the pathophysiology of systemic lupus erythematosus (SLE), although its clinical relevance is still debated. This study aims to assess the connection between serum TNF-α levels and disease activity, as quantified by the Mexican Systemic Lupus Erythematosus Disease Activity Index (MEX-SLEDAI), in Indonesian patients with systemic lupus erythematosus (SLE). A cross-sectional study was performed including 34 patients exhibiting mild-to-moderate systemic lupus erythematosus activity (MEX-SLEDAI ≤12) at Dr. Mohammad Hoesin General Hospital in Palembang, Indonesia. Individuals with significant active disease were excluded. Serum TNF-α concentrations were quantified via a commercial ELISA kit, while disease activity was evaluated through MEX-SLEDAI. Correlation analysis was conducted using Spearman’s rank test, with p < 0.05 being statistically significant. The average age of participants was 33.6 ± 11.0 years, with 94.1% identifying as female. The median MEX-SLEDAI score was 3 (range 2–7), and the median TNF-α level was 1.834 pg/mL (range 0.99–8.62). No substantial connection was detected between serum TNF-α levels and disease activity (r = 0.111, p = 0.533). This suggests that serum TNF-α levels did not correlate with or forecast clinical disease activity in this investigation. The results indicate that the efficacy of TNF-α as a biomarker in SLE may be contingent upon contextual factors, especially illness severity. Additional multicenter and longitudinal studies involving individuals with a broader spectrum of disease activity are necessary to elucidate its clinical significance.

Correlation Between Serum Malondialdehyde Levels and Disease Activity in Patients with Mild to Moderate Systemic Lupus Erythematosus Hartanto, Kristian; Kurniati, Nova; Theodorus; Yuniza; Salim, Eddy Mart
Sriwijaya Journal of Medicine Vol. 8 No. 3 (2025): Vol 8, No 3, 2025 (Issue In Progress)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v8i3.350

Systemic lupus erythematosus (SLE) is characterized by persistent inflammation and oxidative stress, which contribute to the progression of the disease. Lipid peroxidation is indicated by malondialdehyde (MDA). The objective of this investigation was to evaluate the correlation between serum MDA levels and disease activity in patients with mild to moderate systemic lupus erythematosus (SLE). This investigation was a cross-sectional correlation analysis that employed baseline data from adult patients with systemic lupus erythematosus who were receiving conventional treatment. Spearman's correlation was employed to determine the relationship between serum MDA levels and disease activity, which was evaluated using the MEX-SLEDAI score. The study encompassed 38 patients with mild to moderate SLE. The MEX-SLEDAI score was 3 (range 2–7), and the median serum MDA concentration was 1.42 µmol/L (range 0.90–1.99). Spearman's correlation analysis demonstrated a moderately positive correlation between serum MDA levels and MEX-SLEDAI scores (r = 0.415, p = 0.010), indicating that elevated oxidative stress is associated with increased disease activity in SLE patients. A considerable positive correlation between serum malondialdehyde levels and disease activity is statistically significant in patients with mild to moderate systemic lupus erythematosus (SLE). These results support the existence of oxidative stress in the pathophysiology of lupus and suggest that MDA may serve as a potential biomarker for disease monitoring.

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