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All Journal Jurnal Penelitian Pertanian Terapan Jurnal Agro Industri Perkebunan Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science)
Supriyatdi, Dedi
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Agriculture, Biological Sciences & Forestry

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Respons Kalus Embriogenik Tanaman Tebu (Saccharum Officinarum) Var. Kidang Kencana Terhadap Berbagai Modifikasi Media Kultur Dalam Proses Induksi Akar Astuti, Sri Habsari Puji; Indrawati, Wiwik; Supriyatdi, Dedi; Kusuma, Jakty
AGRITROP Vol 18, No 2 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i2.3631

Abstract

Tebu (Saccharum officinarum) merupakan salah satu bahan penghasil gula utama di Indonesia dan memiliki peran penting dalam perekonomian Indonesia dan menjadi sumber devisa negara.  Kemenperin mencatat, produksi gula nasional hanya mencapai angka 2,17 juta ton. Sementara, kebutuhan gula nasional mencapai 66 juta ton. Ini menandakan Indonesia baru mampu memenuhi 3,29% dari total kebutuhan nasional, sehingga lebih dari 96% defisit kebutuhan gula nasional yang belum mampu dan harus dipenuhi Indonesia.. Tujuan dari penelitian ini adalah mendapatkan komposisi media kultur yang tepat dan optimal untuk pertumbuhan akar dari kalus embriogenik tanaman tebu. Penelitian ini bertempat di Laboraturium Kultur Jaringan Politeknik Negeri Lampung. Proses induksi akar ini dilakukan melalui modifikasi media MS dengan campuran IAA, Kinetin, CM, 2,4-D, dan sukrosa yang dilakukan dengan Rancangan Acak Kelompok. Variabel yang diamati adalah respons kalus, waktu tumbuh akar, panjang akar dan jumlah akar. Hasil penelitian dan pengujian menunjukkan bahwa komposisi media berpengaruh terhadap perkembangan akar. Media dengan  kombinasi IAA menghasilkan respons berupa akar, namun media dengan kombinasi 2,4-D memberikan respons berupa pelebaran kalus. Hasil pengamatan menunjukkan perlakuan terbaik untuk pengakaran adalah kombinasi media IAA 1 mg.l-1 baik berupa rata-rata panjang 1,56 cm dan jumlah akar 2,08.
Pengaruh Lama Pelayuan dan Pencacahan Daun Serai Wangi (Cymbopogon winterianus Jowitt ex Bor) pada Rendemen dan Mutu Citronella Oil Gumelar, Ayung Musthafa; Ersan, Ersan; Supriyatdi, Dedi
Jurnal Agro Industri Perkebunan Vol. 10 No. 1 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v10i1.1644

Abstract

Lemongrass plants (Cymbopogon winterianus Jowitt ex Bor), used for their own essential oils through the distillation process. Before distillation process, lemongrass leaves are usually withered to reduce air content, and also reduce their size to be more effectively extraction. The research to study the effect of withering and chopping of citronella leaves before being distilled, on the yield and quality of lemongrass oil produced. The study was conducted in a Randomized Block Design, with factorial arrangement, the first factor withered (0, 1, 2, and 3 x 24 hours) and the second factor was the size of the material (chopped and not chopped). Waterand steam distillation were used. The observations made on the raw material water content, yield, specific gravity, total citronellal, total geraniol, solubility in alcohol, and color. The length of withering affected the yield and total geraniol, the size of the material affected the total geraniol. The length of withering with the size of the material affected the total citronellal, where the total citronellal of the chopped material is higher than the not-chopped, and the longer withering made the total citronellal of citronella oil is higher. The length of withering with the size of the material of citronella leaves does not affect specific gravity, solubility in alcohol, and the color of citronella oil.
Potensi Ekstrak Ubi Gadung (Dioscorea hispida Dennst.) dan Ekstrak Gulma Babadotan (Ageratum conyzoides L.) untuk Pengendalian Hama Penghisap Buah Kakao (Helopeltis spp.) di Laboratorium Supriyatdi, Dedi; Fernando, Adam; Sari, Sismita
Jurnal Agro Industri Perkebunan Vol. 10 No. 2 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v10i2.2590

Abstract

Cocoa pod sucking (Helopeltis spp.) is one of the pests that causes a productivity decrease of cocoa pod in Indonesia. In terms of control, many farmers in Indonesia are still using synthetic insecticides that are not environmentally friendly, so intoxicating yam (Dioscorea hispida Dennts) and tropical whiteweed (Ageratum conyzoides L.) are used as botanical insecticide that are environmentally friendly. The objective of this research is to determine the effect of botanical insecticide that made of intoxicating yam extract, tropical whiteweed extract, and mixed extract of intoxicating yam and tropical whiteweed on the mortality of cocoa pod sucking pests. This research was carried out from November 2020 to February 2021, at the Plant Laboratory II Estate Crop Department, Politeknik Negeri Lampung. The research used a Randomized Block Design (RBD) consisting of four treatments, namely: P0 (control), P1 (intoxicating yam extract), P2 (tropical whiteweed extract), and P3 (combination of both extracts), which were repeated six times. The highest mortality of cocoa pod sucking pests was found in treatment P1 (72.73%). The mortality of cocoa pod sucking pest was caused by extract of intoxicating yam which contains the active ingredients of saponins, alkaloids, flavonoids, and cyanide which enter the digestive system of the cocoa pod sucking pest as stomach poisons, resulting in the death of the cocoa pod sucking pest. Based on the results of the research, it was concluded that intoxicating yam extract is the most potential as botanical insectiside for cocoa pod sucking pest.
Respons Planlet Stevia (Stevia rebaudiana) terhadap Penambahan berbagai Konsentrasi Thidiazuron (TDZ) dan Naphthalene Acetic Acid (NAA) secara in Vitro Dimas, Muhammad; Indrawati, Wiwik; Supriyatdi, Dedi
Jurnal Agro Industri Perkebunan Vol. 11 No. 2 (2023)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v11i2.2849

Abstract

Stevia can be developed by vegetative and generative propagation, while the generative propagation of stevia can be done through seeds. Conventional provision of seeds or seedlings has a low success rate. Propagation via tissue culture is faster, produces more seeds, and is identical to the parent, so the plants’ quality is expected. Plant growth regulator (PGR) often used for tissue culture is type auxins, cytokinins and gibberellins. The function of auxin and cytokinin for division cells, cell elongation, cell differentiation and organ formation. This research aims to get the concentration as well as the best combination for the growth of stevia plants against the administration of thidiazuron (TDZ) and naphthalene acetic acid (NAA). Research carried out by the randomised block design (RBD) factorial pattern consisting of two factors: TDZ and NAA. The first factor is TDZ concentration consisting of three levels: 0 mg.l-1 (T0), 1 mg.l-1 (T1), and 2 mg. l-1 (T2). The second factor is NAA concentration, which consists of three levels: 0 mg.l-1 (N0), 1 mg.l-1 (N1), and 2 mg.l-1 (N1). If there is a difference in data analysis using the F test, then proceed with the BNT level test of 5%. Based on the research results obtained, the influence of TDZ growth regulators and no effect of the concentration of NAA growth regulator on stevia bud multiplication book number. There is an effect of TDZ on plantlet height, leaves, and stevia shoot multiplication nodes.
Co-Authors A.A. Ketut Agung Cahyawan W Astuti, Sri Habsari Puji Dimas, Muhammad Ersan, Ersan Fernando, Adam Gumelar, Ayung Musthafa Hamdani Hamdani Jakty Kusuma, Jakty Martrias, Dina Muhtaria, Charles Nursafuan, Didik ovy erfandari Puspita, Natalia Rachmad Edison, Rachmad Sanjaya, Refky Saty, Fadila Marga Sismita Sari, Sismita Subiantoro, Rijadi Syofian, Mirodi Tamin, Argi Zhafran Wiwik Indrawati, Wiwik Yan Sukmawan, Yan