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PENGARUH SINAR ULTRAVIOLET TERHADAP PERTUMBUHAN BAKTERI Enterotoxigenic E.coli (ETEC) PENYEBAB PENYAKIT DIARE Risky Vidika Apriyanthi; I Gst. AA Ratnawati; Retno Kawuri
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.12157

Abstract

. Telah dilakukan penelitian mengenai pengaruh sinar ultraviolet terhadap pertumbuhan bakteri Enterotoxigenic E.coli  (ETEC). Penelitian dilakukan untuk mengetahui jarak, lama penyinaran, persentase kematian dan pengaruh sinar ultraviolet yang dapat mematikan bakteri ETEC. Metode penanaman dan penghitungan total koloni bakteri yang digunakan adalah plating method. Selanjutnya dilakukan pewarnaan gram untuk melihat bentuk sel bakteri uji.Hasil penelitian menunjukkan, persentase kematian bakteri ETEC tertinggi yaitu pada jarak 15 cm dengan lama penyinaran 15 menit.dengan intensitas 40 lux, sebesar 91,36%. Sedangkan pada jarak 30 cm dengan lama penyinaran 5 menit dan intensitas 19 lux, hanya berhasil membunuh bakteri sebesar 19,52%. Sel bakteri ETEC yang telah diberi penyinaran, bentuk sel bakteri ETEC mengalami perubahan menjadi elips, ada yang mengalami lysis dan susunan rantai terputus. Kata Kunci : Ultraviolet, jarak, lama penyinaran, Enterotoxigenic E.coli  (ETEC).  
Analisis Pigmen Alami Daun Mangga (Mangifera Indicalinn) Sebagai Pewarna Batik Dengan UV-VIS Ni Komang Tribuana Cipta Putri; I Gst Agung Ayu Ratnawati; Wayan Gede Suharta
Kappa Journal Vol 3 No 2 (2019): Desember
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v3i2.1627

Abstract

The development of the batik industry has caused batik consumption to increase. Increased clothing and lifestyle needs trigger the need for practical (synthetic) dyes to have an impact on the waste produced. To reduce negative effects, the use of natural dyes needs to be increased. One of the plants that produce natural dyes is mango (Mangifera Indica Linn). Mango plants (Mangifera Indica Linn) including the family Anacardiaceae. The leaves contain a lot of chlorophyll which can produce color pigments for textiles. To find out the potential, an UV-VIS analysis was carried out, namely absorbance and calculation of the amount of chlorophyll contained in the leaves. The method used is the maceration process with technical ethanol solvents, with variations in concentration (70% and 96%) and variations in the immersion time (3 hours, 5 hours, 10 hours, 20 hours). From the results of UV-VIS spectrophotometry analysis showed the maximum absorption or absorbance at a concentration of 96% for 5 hours and the calculation of chlorophyll by the Lichtenthaler & Welburn (1983) method showed that the sweet arum manggu leaves used for research contained more chlorophyll b than chlorophyll a, gives a greenish-yellow pigment with wavelengths of 540 nm and 640 nm.
PREPARATION, CHARACTERIZATION AND USED OF POLYSULFONE MEMBRANES FOR THE TREATMENT OF WATER SOLUTIONS CONTAINING HUMIC ACIDS Ni Nyoman Rupiasih; Made Sumadiyasa; A.A. Ratnawati; Hery Suyanto
Jurnal Sains Materi Indonesia VOL 13, NO 1: OKTOBER 2011
Publisher : Center for Science & Technology of Advanced Materials - National Nuclear Energy Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.879 KB) | DOI: 10.17146/jusami.2011.13.1.5400

Abstract

PREPARATION, CHARACTERIZATION AND USED OF POLYSULFONE MEMBRANES FOR THE TREATMENT OF WATER SOLUTIONS CONTAINING HUMIC ACIDS. Polysulfone (PSf) membranes were cast by phase inversion method using PSf as matrix, N,N-Dimethylacetamide (DMAc) as solvent and distilled water as coagulant. The microporous membrane has studied using Scanning Electron Microscope (SEM) and filtration techniques. The filtration experiments were performed on a flat sheet membrane using pure water and humic acids (HA) solution as feeds. The results show, the Pure Water Flux (PWF) and Product Flux (PF) values decrease with increasing thickness of the membranes. They are consistent with the SEM images that thicker membranes have smaller pore size and less in numbers. The Rejection coefficient (R) values showthat the membranes are able to filter HA in the form of solution, with the performance dependent on the characteristics: thickness and microporous structure. They were increased with increase in the thickness of the membranes and with increase in the wavelength from 250-665 nm. It suggests that, the PSf membranes can be used to minimize the amount of Humic Acids from water process to improve the quality of treated to an acceptable quality level.
Analisis Diagnostic Reference Level (DRL) Pasien Pada Pemeriksaan Abdomen Kontras dan Nonkontras Dengan Pesawat CT Scan Di RSUD Bali Mandara Br Ginting, Venesia Sesilia; Sutapa, Gusti Ngurah; Ratnawati, I Gusti Agung Ayu; Widiana, I Komang
Kappa Journal Vol 7 No 1 (2023): April
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v7i1.18985

Abstract

In this study, data were obtained from 35 patients aged 27-76 years. The current used varies from 46-185 mAs and a constant tube voltage of 130 kV, with different clinical symptoms. The patient's DRL value on contrast and non-contrast abdominal examination was determined by calculating the third quartile value. On non-contrast abdominal examination, the DRL CTDIvol value was 10.62 mGy and the DLP DRL was 607.76 mGy.cm and on the non-contrast abdominal examination, the DRL CTDIvol value was 32.73 mGy and the DRL DLP value was 1825.10 mGy.cm. These results indicate that the DRL CTDI vol and DRL DLP values on contrast abdominal examination at Bali Mandara Hospital were still below the actor's limits set by BAPETEN, while on abdominal examination the DRL CTDI vol and DRL DLP values exceeded the limits set by BAPETEN. This is caused by exposure which is done three times. Therefore, it is necessary to restore both the Patient Operational Standards (SOP) and the exposure factor on an abdominal contrast CT scan at Bali Mandara Hospital.
Analisis Laju Dosis Radiasi di Area Ruang CT_Scan di Instalasi Radiologi RSU Bali Mandara Else Putri Ayu Prameswari; Gusti Ngurah Sutapa; I Gusti Agung Ayu Ratnawati; Ni Nyoman Ratini; I Nengah Artawan; Ni Luh Putu Trisnawati
Kappa Journal Vol 8 No 2 (2024): Kappa Journal
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v8i2.25211

Abstract

Research has been carried out on radiation dose rate analysis in the CT-SCAN area of the radiology room at RSU Mandara Bali. The data used in this research is quantitative data observed from the radiation rate from the CT-SCAN aircraft. This study aims to determine the radiation dose rate in the radiology area and its rationalization based on regulations regarding radiation NBD (dose limit values) that apply to occupational safety and health. The research data is the actual radiation dose rate determined from the dose rate and calibration factors. The results of data calculations show that the highest dose rates are respectively at T2, 30 cm, 50 cm and 100 cm (door) with values of 1.95, 0.94 and 0.05 μSv/year, while the lowest dose rates are respectively at T4, 30 cm, 50 cm and 100 cm (waiting room) with values of 0.08, 0.06 and 0.05 μSv/year. The research results show that the dose rate at each measurement point is still considered safe for radiation workers where the NBD received is still below 20 μSv/year, while the dose rate is classified as safe for the general public at measuring distances of 50 cm and 100 cm, where the NBD received is below 1 μSv/year assuming the community concerned acts as a patient or patient's family with an interest in the radiology room area.
Measurement of Natural and Artificial Radiation as Well as Radiation Applications in The Field of Medical Physics at SMA Negeri II Kuta Badung Bali Gusti Ngurah Sutapa; I Gde Antha Kasmawan; Ida Bagus Made Suryatika; I Made Yuliara; Ni Nyoman Ratini; I Gusti Agung Ayu Ratnawati; Ni Luh Putu Trisnawati; I Ketut Putra; Ni Kadek Nova Anggarani
Kappa Journal Vol 8 No 3 (2024): Kappa Journal
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v8i3.28254

Abstract

The results of research that have been carried out, especially in the field of medical physics such as X-rays, have been widely utilized in the medical field, especially Radiology Units in Several General and Regional Hospitals. Thus, it is very important to be understood and known by the community, especially the community, in this case the students of SMA Negeri 2 Kuta. In 2022/2023, there are 507 male and 597 female students. Seeing these conditions, surveys and observations were carried out as a result, students had difficulty understanding physics because the physics subject matter was dense, memorized and mathematical. Seeing these results, it is necessary to carry out a new paradigm in physics learning. One of the more appropriate methods is to balance theory learning with field applications such as natural and artificial radiation measurements and applications in the field of medical physics. The results of natural radiation measurements in the classroom and outside the classroom were 0.605 ± 0.0024 and 0.182 ± 0.0008 mSv/year, respectively. The limit of the dosage rate allowed by BAPETEN is 1,000 mSv/year. Natural radiation identified in the classroom and outside the classroom is still declared safe
Uji Kandungan Limbah Tahu Menggunakan Spektroskopi Fourier Transform Infrared Dan Laser-Induced Breakdown Spectroscopy Di Tukad Buaji Kelurahan Sesetan Denpasar Selatan Novita Susanti Elsi; I Gusti Agung Ayu Ratnawati; Wayan Gede Suharta; Ketut Putra; Anak Agung Ngurah Gunawan; I Nengah Sandi
Kappa Journal Vol 9 No 1 (2025): Kappa Journal
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v9i1.28604

Abstract

Telah dilakukan penelitian dengan judul uji kandungan limbah tahu menggunakan spektroskopi Fourier Transform Infrared dan Laser Induced Breakdown Spectroscopy di Tukad Buaji Kelurahan Sesetan Denpasar Selatan. Limbah yang dihasilkan pada proses produksi tahu pada umumnya dibagi menjadi dua bentuk limbah, diantaranya adalah limbah padat dan limbah cair. Jenis limbah tahu yang di uji pada penelitian ini adalah limbah cair, hal ini disebabkan karena limbah cair tahu banyak menghasilkan permasalahan terhadap lingkungan yang dilaluinya terutama aliran air sungai. Penelitian ini bertujuan untuk mengetahui kadar dari BOD, COD, pH, mengindentifikasi gugus fungsi serta mengetahui unsur apa yang terkandung di dalam limbah cair tahu. Metode pengujiannya dilakukan dengan menggunakan metode titrimetri, spektroskopi FTIR dan LIBS. Berdasarkan hasil pengujian dengan metode titrimetri pada limbah tahu adalah diperoleh perhitungan rata-rata dan standar deviasi untuk kadar BOD sebesar 13,306 7,886 mg/L (hasil ini masih dibawah standar baku mutu), kadar COD sebesar 28,416 12,908 mg/L (hasil ini masih dibawah standar baku mutu) dan pH sebesar 7,55 0,02 (hasil dalam kisaran baku mutu). Hasil pengujian dengan FTIR menunjukkan adanya gugus fungsi O-H, O=C=O, C=O dan C-H pada limbah tahu, sedangkan hasil uji yang diperoleh dengan menggunakan LIBS adalah terdapat unsur Ca, Na, K, W, Cd dan Cu yang terkandung di dalam limbah tahu.