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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education PRIMA: Journal of Community Empowering and Services SEMAR Eduproxima : Jurnal Ilmiah Pendidikan IPA
Sari, Siti Lusi Arum
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Physics Social Sciences

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Isolation and Identification of α-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Susilowati, Ari; Dewi, Citra Praba Yunita; Sari, Siti Lusi Arum
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Abstract

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce ?-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing ?-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor ?-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its ?-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards ?- glycosidase analysis was performed using the spectrophotometric method (? = 415 nm), with p-nitro phenyl ?-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence.  The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.
Lipid from microalgae isolated from Cengklik Reservoir, Boyolali, Central Java for prospective biodiesel sources Sari, Siti Lusi Arum; Suratman, Suratman; Sunarto, Sunarto; Purwoko, Tjahjadi; Larasati, Dewi; Herina, Stefanus Junliem Nugratama
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.55910

Abstract

The availability and sustainability of the algae-based biodiesel industry depend on the adequate selection of microalgae strains. Based on this, it is important to explore local microalgae from Indonesia as a feedstock for biodiesel. This study aimed to isolate and screen freshwater microalgae with lipid-producing abilities. A total of three microalgal isolates have been successfully isolated from the Cengklik Reservoir (-7.506712, 110.723582). Based on the cell morphology, the three isolates were identified as Chlorella sp., Scenedesmus sp., and Oscillatoria sp. Nile red staining indicated that the three microalgal isolates contained neutral lipid droplets in their cells, as shown by the presence of a yellow or orange luminescence under a fluorescence microscope. The results of this study indicated that Scenedesmus sp. and Oscillatoria sp. had more potential as feedstocks for biofuels due to their higher lipid content and productivity. This research succeeded in obtaining microalgae isolates from Cengklik reservoir, Boyolali, Central Java which can be used as a source of lipids for biodiesel.
Arrowroot (Maranta arundinacea L.) Tuber Extract Modulated Gut Microbiota and Improved Glycemic Control in Type 2 Diabetic Mice Pangastuti, Artini; Anggraini, Putri Regita; Listyawati, Shanti; Sari, Siti Lusi Arum
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.371-381

Abstract

Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by hyperglycemia and has become a global health concern. Gut microbiota alterations significantly influence diabetes onset, which can be mitigated through prebiotic intake. Arrowroot tubers (Maranta arundinacea L.) possess prebiotic potential due to their oligosaccharide and dietary fiber content. This study evaluated the effects of arrowroot tuber extract on blood glucose, glucose tolerance, and gut microbiota composition in a T2DM mouse model. Tubers were macerated in ethanol, and BALB/c mice were fed a high-fat diet for five weeks, followed by streptozotocin injection (35 mg/kg) to induce T2DM. Mice received 150 mg of arrowroot tuber extract orally for 28 days. Blood glucose levels were measured on days 0, 14, and 28, and an oral glucose tolerance test (OGTT) was performed. Cecal samples collected after four weeks underwent 16S rRNA sequencing, and data were analyzed using One-Way ANOVA (p<0.05). Treatment with arrowroot tuber extract for 28 days reduced blood glucose levels by 40.5% and improved glucose tolerance. The extract modulated gut microbiota, decreasing harmful bacteria while increasing short-chain fatty acid (SCFA)-producing genera such as Lachnospiraceae NK4A136, Ligilactobacillus, and Alistipes. These results indicate that arrowroot tuber extract can beneficially alter gut microbiota composition and ameliorate hyperglycemia, suggesting its potential as a prebiotic-based therapeutic strategy for managing T2DM.
RESPON PERTUMBUHAN DAN KANDUNGAN FLAVONOID BUNGA TELANG (Clitoria ternatea L.) BIRU, PUTIH, UNGU, DAN BIRU MUDA TERHADAP CEKAMAN SALINITAS Tyas, Berliyana Wahyu Ning; Purwanto, Edi; Sari, Siti Lusi Arum
EDUPROXIMA : Jurnal Ilmiah Pendidikan IPA Vol 7, No 4 (2025)
Publisher : Universitas Bhinneka PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29100/.v7i4.9409

Abstract

Bunga telang merupakan salah satu tanaman yang menjadi alternatif pewarna alami dan mengandung flavonoid tinggi yang berpotensi sebagai antioksidan yang baik bagi kesehatan. Bunga telang yang dibudidayakan di Indonesia memiliki warna mahkota bunga biru, putih, ungu, dan biru muda. Namun belum ada penelitian yang  menjelaskan bahwa bunga telang dengan warna bunga yang berbeda memiliki respon berbeda terhadap lingkungan. Berdasarkan hal tersebut, tujuan penelitian ini untuk mengetahui bagaimana respon bunga telang biru, putih, ungu, dan biru muda terhadap cekaman salinitas, pada parameter pertumbuhan dan kandungan flavonoid. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri atas dua faktor yaitu variasi warna bunga telang (biru; putih; ungu; biru muda) dan konsentrasi larutan garam NaCl (0; 2500; 5000 ppm). Hasil penelitian menunjukkan perlakuan NaCl pada variasi warna bunga telang berpengaruh signifikan terhadap (panjang akar, jumlah daun, jumlah bunga, tinggi tanaman). Bunga telang biru (2500 ppm) menghasilkan kandungan flavonoid tertinggi. Serta perlakuan NaCl 2500 ppm merupakan perlakuan optimum untuk meningkatkan parameter kandungan flavonoid seluruh variasi warna bunga telang. Sedangkan bunga telang putih (5000 ppm) menghasilkan kandungan flavonoid terendah dan perlakuan NaCl 5000 ppm merupakan perlakuan yang menurunkan kandungan flavonoid pada seluruh variasi warna bunga telang.
Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels Sari, Siti Lusi Arum; Tanjung, Wida Puspa; Amilia, Klara Rizky; Setyaningsih, Ratna; Pangastuti, Artini
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 1 (2024): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i1.77610

Abstract

The application of pectinase in industries will continue to be developed. To broaden the applicability of enzyme manufacturing in new industries, more research to investigate pectinolytic microorganisms with high activity and stability is still required. Therefore, this study aimed to obtain pectinolytic fungi that have the potential as pectinase producers. Pectinolytic fungi were isolated from rotten apple peels and selected based on clear zone formation on pectic agar media after cetyl trimethyl ammonium bromide (CTAB) staining. Pectinolytic fungi were identified based on 18S rRNA partial gene sequences and morphological characteristics. Pectinase production used Mandels and Weber medium with citrus pectin 20 g l-1 addition. Pectinase activity was determined based on the measurement of reducing sugars by a colorimetric method. A total of 5 fungal isolates were successfully isolated. All of the isolates had pectinolytic activities with clear zone diameters ranging between 0.99 to 7.32 mm. The isolate A3 showing the highest pectinolytic activity was identified as Rhizopus stolonifer. Microscopically, R. stolonifer A3 showed typical characteristics for Rhizopus, characterized by the presence of rhizoids, stolons and sporangiophores bearing a single spherical sporangium. The pectinase production of R. stolonifer A3 was optimum at initial pH 5.0, temperature 35 °C and incubation period of 3 days with pectinase activity of 14.75 U ml-1. R. stolonifer A3 produced acidic pectinase having optimum activity at pH 5.0 and temperature 50 °C. Thus R. stolonifer A3 has the potential to be used as a producer of acidic pectinase appropriate for use in the processing of fruit products.
Produksi Nata de Whey: Solusi Penanganan Limbah Keju dari PT. Mazaraat Lokanatura Indonesia dan Pemberdayaan Masyarakat Sari, Siti Lusi Arum; Artini, Pangastuti; Ratna, Setyaningsih; Ari, Susilowati; Tjahjadi, Purwoko; Permana, Muhammad Fajar
PRIMA: Journal of Community Empowering and Services Vol 9, No 2 (2025): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v9i2.80055

Abstract

Nata de Whey Production: Solutions for Handling Cheese Waste from PT. Mazaraat Lokanatura Indonesia and Community Empowerment. One of the problems faced by PT Mazaraat Lokanatura Indonesia is the abundant liquid cheese waste (whey). If not properly managed, cheese whey could be harmful to the  environment. Whey still contains a lot of milk nutrients so it can be used as a substrate for the production of nata de whey. Based on this, the purpose of this activity was to produced nata de whey from which was served as a nata-salak cocktail. This activity involves 2 partners, namely PT Mazaraat Lokanatura Indonesia and the Tegalrandu community, Srumbung, Magelang. The activity was carried out in an integrated manner with the KKN MBKM Biology Study Program, FMIPA UNS in Tegalrandu, Srumbung, Magelang. The stages of the activity were: socialization, provision of KKN students, training in making nata and cocktails, and mentoring. Training starts from whey preparation, fermentation, processing nata into cocktails, and packaging. Mentoring and monitoring activities were carried out directly by the KKN student group. Socialization and FGD with PT Mazaraat were held on June 26, 2023. Through this FGD, the management of PT. Mazaraat supports the use of whey by the community. Socialization with the Tegalrandu community were carried out in an integrated manner with the implementation of the MBKM KKN program. Before the training, KKN students had practiced making Nata and cocktails. The Tegalrandu village community was very enthusiastic about participating in the training activities for making nata de whey and nata-salak cocktails. Through this activity, A nata-salak cocktail product that may be utilized as an unique and extraordinary Tegalrandu village product was created. This activity provides an alternative solution to the waste problem of PT Mazaraat Lokanatura and opens up business opportunities for the Tegalrandu, Srumbung, Magelang community.
Budidaya Magot Black Soldier Fly di Desa Donohudan, Ngemplak, Boyolali, Jawa Tengah: Solusi Inovatif Permasalahan Sampah Sari, Siti Lusi Arum; Anjani, Amanda Lestania Diva; Astuti, Anggra Puspita; Fitri, Anissa; Kirana, Candrastami; Arifin, Diva Calista; Nuraini, Farida Mira; Maharani, Ferlyana Putri; Wijayanti, Gita Tri; Dewata, Gregorious Tirta; Wulandari, Jaitun Putri Eka; Febriliana, Lutfi; Yulyanto, Muhammad Kunto Dwi; Purba, Priskila Natasya Rambe; Qorina, Qurrota A’yunin; Fitriyanti, Rindiani; Nabiila, Zakiyyatun Nisa’ Althoofun; Addiniyyah, Amirza Fahma; Praptiningtyas, Ellisabeth; Cahyawati, Nilam; Purba, Ibnu Tryansar
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 14, No 1 (2025): Mei
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v14i1.90916

Abstract

Desa Donohudan,kecamatan Ngemplak, Boyolali secara langsung  berbatasan dengan kota Surakarta sehingga menjadi salah satu area pengembangan perekonomian kota Surakarta ke arah barat. Perkembangan ekonomi berkorelasi dengan peningkatan jumlah penduduk dan berdampak pada peningkatan produksi sampah organik. Sampah organik   jika tidak dikelola dengan baik akan berdampak pada penurunan kualitas lingkungan dan kesehatan Masyarakat.   Teknologi MBSF Black Soldier Fly (MBSF) merupakan solusi inovatif untuk pengolahan sampah organik.  Selain dapat mengatasi permasalahan sampah, MBSF yang dihasilkan memiliki nilai ekonomi. Berdasarkan hal tersebut kelompok 12 KKN Universitas Sebelas Maret periode Januari-Februari 2024 melakukan kegiatan pelatihan budidaya dan pengolahan pasca panen MBSF sebagai upaya mengatasi permasalahan sampah sekaligus memberikan peluang pemberdayaan Masyarakat.Kegiatan ini dilakukan melalui beberapa tahap yaitu: persiapan, sosialisasi, pelatihan, pendampingan, dan evaluasi. Kegiatan diikuti oleh warga desa Donohudan yang tergabung dalam kelompok tani Karya Maju. Setelah penyampaian materi dan praktek bersama dalam pelatihan, warga juga melakukan praktek mandiri dengan didampingi tim KKN klp 12. Kegiatan ini berhasil mentransfer pengetahuan dan ketrampilan tentang budidaya dan pengolahan pasca panen MBSF kepada masyarakat desa Donohudan. Produk yang dihasilkan berupa tepung MBSF dalam kemasan yang diberi label merek PATEN. Tepung MBSF ini dapat dimanfaatkan sendiri sebagai bahan tambahan pakan ternak atau  dijual untuk meningkatkan pendapatan.
Co-Authors Addiniyyah, Amirza Fahma Amilia, Klara Rizky Anggraini, Putri Regita Anjani, Amanda Lestania Diva ARI SUSILOWATI Ari, Susilowati Arifin, Diva Calista ARTINI PANGASTUTI Artini, Pangastuti Astuti, Anggra Puspita Cahyawati, Nilam Dewata, Gregorious Tirta Dewi Larasati, Dewi Dewi, Citra Praba Yunita Edi Purwanto Febriliana, Lutfi Fitri, Anissa Fitriyanti, Rindiani Herina, Stefanus Junliem Nugratama Kirana, Candrastami Maharani, Ferlyana Putri Nabiila, Zakiyyatun Nisa’ Althoofun Nuraini, Farida Mira Permana, Muhammad Fajar Praptiningtyas, Ellisabeth Purba, Ibnu Tryansar Purba, Priskila Natasya Rambe Qorina, Qurrota A’yunin RATNA SETYANINGSIH Ratna, Setyaningsih Shanti Listyawati Sunarto Sunarto Suratman Suratman Tanjung, Wida Puspa TJAHJADI PURWOKO Tjahjadi, Purwoko Tyas, Berliyana Wahyu Ning Wijayanti, Gita Tri Wulandari, Jaitun Putri Eka Yulyanto, Muhammad Kunto Dwi