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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi Biosaintifika: Journal of Biology & Biology Education Eduproxima : Jurnal Ilmiah Pendidikan IPA
Sari, Siti Lusi Arum
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Immunology & microbiology Physics

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Isolation and Identification of α-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Susilowati, Ari; Dewi, Citra Praba Yunita; Sari, Siti Lusi Arum
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Abstract

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce ?-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing ?-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor ?-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its ?-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards ?- glycosidase analysis was performed using the spectrophotometric method (? = 415 nm), with p-nitro phenyl ?-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence.  The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.
Lipid from microalgae isolated from Cengklik Reservoir, Boyolali, Central Java for prospective biodiesel sources Sari, Siti Lusi Arum; Suratman, Suratman; Sunarto, Sunarto; Purwoko, Tjahjadi; Larasati, Dewi; Herina, Stefanus Junliem Nugratama
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.55910

Abstract

The availability and sustainability of the algae-based biodiesel industry depend on the adequate selection of microalgae strains. Based on this, it is important to explore local microalgae from Indonesia as a feedstock for biodiesel. This study aimed to isolate and screen freshwater microalgae with lipid-producing abilities. A total of three microalgal isolates have been successfully isolated from the Cengklik Reservoir (-7.506712, 110.723582). Based on the cell morphology, the three isolates were identified as Chlorella sp., Scenedesmus sp., and Oscillatoria sp. Nile red staining indicated that the three microalgal isolates contained neutral lipid droplets in their cells, as shown by the presence of a yellow or orange luminescence under a fluorescence microscope. The results of this study indicated that Scenedesmus sp. and Oscillatoria sp. had more potential as feedstocks for biofuels due to their higher lipid content and productivity. This research succeeded in obtaining microalgae isolates from Cengklik reservoir, Boyolali, Central Java which can be used as a source of lipids for biodiesel.
Arrowroot (Maranta arundinacea L.) Tuber Extract Modulated Gut Microbiota and Improved Glycemic Control in Type 2 Diabetic Mice Pangastuti, Artini; Anggraini, Putri Regita; Listyawati, Shanti; Sari, Siti Lusi Arum
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.371-381

Abstract

Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by hyperglycemia and has become a global health concern. Gut microbiota alterations significantly influence diabetes onset, which can be mitigated through prebiotic intake. Arrowroot tubers (Maranta arundinacea L.) possess prebiotic potential due to their oligosaccharide and dietary fiber content. This study evaluated the effects of arrowroot tuber extract on blood glucose, glucose tolerance, and gut microbiota composition in a T2DM mouse model. Tubers were macerated in ethanol, and BALB/c mice were fed a high-fat diet for five weeks, followed by streptozotocin injection (35 mg/kg) to induce T2DM. Mice received 150 mg of arrowroot tuber extract orally for 28 days. Blood glucose levels were measured on days 0, 14, and 28, and an oral glucose tolerance test (OGTT) was performed. Cecal samples collected after four weeks underwent 16S rRNA sequencing, and data were analyzed using One-Way ANOVA (p<0.05). Treatment with arrowroot tuber extract for 28 days reduced blood glucose levels by 40.5% and improved glucose tolerance. The extract modulated gut microbiota, decreasing harmful bacteria while increasing short-chain fatty acid (SCFA)-producing genera such as Lachnospiraceae NK4A136, Ligilactobacillus, and Alistipes. These results indicate that arrowroot tuber extract can beneficially alter gut microbiota composition and ameliorate hyperglycemia, suggesting its potential as a prebiotic-based therapeutic strategy for managing T2DM.
RESPON PERTUMBUHAN DAN KANDUNGAN FLAVONOID BUNGA TELANG (Clitoria ternatea L.) BIRU, PUTIH, UNGU, DAN BIRU MUDA TERHADAP CEKAMAN SALINITAS Tyas, Berliyana Wahyu Ning; Purwanto, Edi; Sari, Siti Lusi Arum
EDUPROXIMA : Jurnal Ilmiah Pendidikan IPA Vol 7, No 4 (2025)
Publisher : Universitas Bhinneka PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29100/.v7i4.9409

Abstract

Bunga telang merupakan salah satu tanaman yang menjadi alternatif pewarna alami dan mengandung flavonoid tinggi yang berpotensi sebagai antioksidan yang baik bagi kesehatan. Bunga telang yang dibudidayakan di Indonesia memiliki warna mahkota bunga biru, putih, ungu, dan biru muda. Namun belum ada penelitian yang  menjelaskan bahwa bunga telang dengan warna bunga yang berbeda memiliki respon berbeda terhadap lingkungan. Berdasarkan hal tersebut, tujuan penelitian ini untuk mengetahui bagaimana respon bunga telang biru, putih, ungu, dan biru muda terhadap cekaman salinitas, pada parameter pertumbuhan dan kandungan flavonoid. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri atas dua faktor yaitu variasi warna bunga telang (biru; putih; ungu; biru muda) dan konsentrasi larutan garam NaCl (0; 2500; 5000 ppm). Hasil penelitian menunjukkan perlakuan NaCl pada variasi warna bunga telang berpengaruh signifikan terhadap (panjang akar, jumlah daun, jumlah bunga, tinggi tanaman). Bunga telang biru (2500 ppm) menghasilkan kandungan flavonoid tertinggi. Serta perlakuan NaCl 2500 ppm merupakan perlakuan optimum untuk meningkatkan parameter kandungan flavonoid seluruh variasi warna bunga telang. Sedangkan bunga telang putih (5000 ppm) menghasilkan kandungan flavonoid terendah dan perlakuan NaCl 5000 ppm merupakan perlakuan yang menurunkan kandungan flavonoid pada seluruh variasi warna bunga telang.
Co-Authors Anggraini, Putri Regita ARI SUSILOWATI ARTINI PANGASTUTI Dewi Larasati, Dewi Dewi, Citra Praba Yunita Edi Purwanto Herina, Stefanus Junliem Nugratama Shanti Listyawati Sunarto Sunarto Suratman Suratman TJAHJADI PURWOKO Tyas, Berliyana Wahyu Ning