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Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices Dewi, Kifayati Rosiyanti; Kusnandar, Feri; Yuliana, Nancy Dewi; Ismayati, Maya; Solihat, Nissa Nurfajrin; Riantana, Handy; Heryani, Heryani
Indonesian Journal of Halal Research Vol 5, No 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21191

Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultrasound) before LC-MS/MS analysis to determine the most effective method for detecting marker peptide targets from porcine gelatin. The appropriate method was applied to isolate porcine gelatin peptides in the matrices of bovine gelatin and confectionery products (lozenges, marshmallows, and soft candy) at concentrations of 0.01, 0.1, and 1% (w/w). Relative detection limit values were determined. The results showed that conventional digestion treatment yielded a higher marker peptide detection rate than microwave and ultrasound digestion. Meanwhile, the detection limit of porcine gelatin in bovine gelatin ranged from 0.09 to 0.89%, depending on the marker peptide used, and could be significantly detected at a concentration of 1% in the confectionery product. The marker peptide TGQPGAVGPAGIR exhibited the highest stability, as it was detectable at the lowest concentration across all mixed matrices. The LC-MS/MS method has been proven to afford sensitive results and has the potential to serve as an alternative for detection of halal status.

Detection of Lard in Animal Fat Mixtures Using ATR-FTIR Fingerprint and SPME-GC/MS-Based Volatilomics Putri, Silmiyah; Budi, Faleh Setia; Suseno, Sugeng Heri; Heryani, Heryani; Ramadhan, Muhamad Fauzi; Regiyana, Yane; Yuliana, Nancy Dewi
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90240

This study aims to detect the presence of lard in several halal animal fats (beef, chicken, and goat fat) based on their infrared fingerprint and volatile compound profile (volatilomics). A mixture of fat samples obtained from halal animals and lard at different concentrations (0, 20, 40, 60, and 80%, v/v) were subjected to attenuated total reflection-Fourier transformed infrared spectroscopy (ATR-FTIR) and solid phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GC/MS) analysis, respectively. The data was processed using orthogonal projection to the least square–discriminant analysis (OPLS-DA). The results showed that ATR-FTIR could only identify the presence of lard in chicken fat up to the lowest concentration used in this study (10%) but failed in other fat samples. SPME-GC/MS detected the presence of lard in all animal fats up to the lowest concentration added (10%). The results of this study revealed that the volatilomics technique had more potential to be developed as a basis for the rapid detection of halal and non-halal animal fat than the infrared fingerprint. This study also emphasized that markers of non-halal animal fats can be different when the same fats are added to different food products.

Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices Dewi, Kifayati Rosiyanti; Kusnandar, Feri; Yuliana, Nancy Dewi; Ismayati, Maya; Solihat, Nissa Nurfajrin; Riantana, Handy; Heryani, Heryani
Indonesian Journal of Halal Research Vol. 5 No. 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21191

Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultrasound) before LC-MS/MS analysis to determine the most effective method for detecting marker peptide targets from porcine gelatin. The appropriate method was applied to isolate porcine gelatin peptides in the matrices of bovine gelatin and confectionery products (lozenges, marshmallows, and soft candy) at concentrations of 0.01, 0.1, and 1% (w/w). Relative detection limit values were determined. The results showed that conventional digestion treatment yielded a higher marker peptide detection rate than microwave and ultrasound digestion. Meanwhile, the detection limit of porcine gelatin in bovine gelatin ranged from 0.09 to 0.89%, depending on the marker peptide used, and could be significantly detected at a concentration of 1% in the confectionery product. The marker peptide TGQPGAVGPAGIR exhibited the highest stability, as it was detectable at the lowest concentration across all mixed matrices. The LC-MS/MS method has been proven to afford sensitive results and has the potential to serve as an alternative for detection of halal status.

Aktivitas Jus Buah Delima (Punica granatum L.) terhadap Peroksidasi Lipid Darah Tikus yang Diinduksi Parasetamol Roswiem, Anna Priangani; Heryani, Heryani; Apriliana, Dian
Jurnal EnviScience (Environment Science) Vol. 2 No. 2 (2018)
Publisher : Universitas Islam Lamongan

Buah delima atau pomegranat (Punica granatum L.) telah dilaporkan memiliki aktivitas antioksidan lebih tinggi daripada tanaman obat lainnya. Aktivitas antioksidan berpotensi menurunkan kadar lipid peroksida (Tiobarbituric Acid Reacting Substances/TBARS). TujuanÂ penelitian ini adalah untuk menguji aktivitas jus buah delima terhadap peroksidasi lipid darah tikus yang diinduksi parasetamol. Empat belas ekor tikus dibagi dalam empat kelompok. Kelompok normal (N) dicekok akuades dan tiga kelompok lainnya (kelompok kontrol positif (PC), kelompok kontrol negatif (KN), dan kelompok jus buah delima (JUS) diinduksi parasetamol (500 mg/kgBB) selama 34 hari. Setelah 17 hari, kelompok yang diinduksi parasetamol, yaitu kelompok kontrol positif (PC) diinduksi Cursil 70 Â dengan dosis (13,3 mg/kgBB), kelompok jus buah delima Â diinduksi jus buah delima (JUS) dengan dosis (500 mg/kgBB), tetapi kelompok kontrol negatif (KN) tetap diinduksi parasetamol (500 mg/kgBB) sampai dengan hari keâ€“34. Lipid peroksida darah direaksikan dengan asam tiobarbiturat (TBA)1% dan dianalisis dengan spektrofotometer pada Î» 352 nm. Kontrol negatif (KN) yang diinduksi oleh parasetamol selama 34 hari, meningkatkan TBARS sebesar 120,66%. Kadar lipid peroksida (TBARS) kelompok PC dan kelompokÂ JUS dengan dosis (500 mg/kgBB) menurun secara berturut-turut sebesar 8,14% dan 54,79%.

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