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Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.

POTENSI KONSORSIUM Bacillus spp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI RAWIT Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

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Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman hortikultura yang bernilai ekonomi tinggi. Produksi cabai rawit seringkali terkendala oleh penyakit layu batang yang diakibatkan cendawan Fusarium sp. Penelitian ini bertujuan untuk mengetahui pengaruh konsorsium bakteri Bacillus spp. dan Pseudomonas fluorescens sebagai agensia hayati pengendali penyakit layu fusarium pada tanaman cabai rawit. Penelitian ini dilaksanakan di screenhouse UPN “Veteran” Jawa Timur. Penelitian dimulai pada bulan Januari 2021 sampai bulan April 2021. Penelitian disusun menggunakan Rancangan Petak Terbagi (RPT) dengan dua faktor yaitu bakteri antagonis dan waktu aplikasi dan diulang sebanyak 4 kali. Faktor pertama yaitu bakteri antagonis dengan 5 taraf perlakuan yaitu Tanpa pemberian bakteri antagonis, Bacillus spp. kode isolat Ba9, Bacillus spp. kode isolat Ba17, P. fluorescens, Bacillus spp. kode isolat Ba9 + P. fluorescens, Bacillus spp. kode isolat Ba17 + P. fluorescens. Faktor kedua yaitu waktu aplikasi dengan 2 taraf perlakuan yaitu sebelum pindah tanam bibit dan setelah pindah tanam bibit. Jika diketahui adanya pengaruh yang nyata dari perlakuan maka dilakukan Uji Jarak Ganda Duncan atau Duncan Multiple Range Test (DMRT) pada taraf 5%. Perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba9 dan P. fluorescens menyebabkan penundaan masa inkubasi Fusarium sp. sebesar 23,42%, meningkatkan tinggi tanaman sebesar 20,55 %, dan mempercepat munculnya calon bunga, sedangkan perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba17 dan P. fluorescens menekan intensitas penyakit layu fusarium 29,03%, meningkatkan jumlah daun sebesar 56,58% dibandingkan dengan pemberian bakteri secara tunggal.

POTENSI BAKTERI Bacillus spp. DALAM MENGHAMBAT Colletotrichum capsici PENYEBAB ANTRAKNOSA PADA CABAI MERAH SECARA IN VITRO Sasiska Rani; Endang Triwahyu Prasetyawati; Herry Nirwanto
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

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Colletotrichum capsici merupakan jamur patogen penting penyebab penyakit antraknosa pada cabai merah. Salah satu upaya untuk mengatasi permasalahan ini yaitu dengan penggunaan agens hayati bakteri Bacillus spp.. Penelitian ini bertujuan untuk mengetahui potensi lima isolat bakteri Bacillus spp. yaitu Ba-6, Ba-9, Ba-12, Ba-15, dan Ba-17 dalam menghambat patogen C. capsici Penelitian laboratorium dilakukan menggunakan Rancangan Acak Lengkap dengan 4 ulangan. Pengujian in vitro dilakukan dengan teknik dual culture pada media PDA dan detach fruit test pada buah cabai.Hasil penelitian menunjukkan efektifitas perhambatan C. capsici paling baik pada pengujian in vitro media PDA yaitu Bacillus sp. isolat Ba-9 dengan penghambatan sebesar 23,04%. Selanjutnya dalam uji detach fruit, Bacillus sp. isolat Ba-9 mampu menekan pertumbuhan C. capsici sebesar 21,25% dibandingkan kontrol.

Potensi Streptomyces sp. Terhadap Penurunan Virulensi Bakteri Ralstonia solanacearum dalam Skala In vitro Sa'adah, Fatimah Lailatus; Suryaminarsih, Penta; Prasetyawati, Endang Triwahyu
Jurnal Agroekoteknologi Vol 16, No 1 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i1.23238

The demand for chili peppers in Indonesia continues to increase, but in 2021 there was a decrease in chili production. One of the main causes is pathogen attack, especially Ralstonia solanacearum which causes bacterial wilt disease in chili plants. This study aims to evaluate the potential of Streptomyces sp. isolates BMP and TMP to reduce the virulence of R. solanacearum. The research was conducted through a series of laboratory experiments, including an inhibition test with double-layer method and virulence reduction test on Triphenyl Tetrazolium Chloride (TZC) media. The results showed that the TMP isolate was able to produce an inhibition zone of 1.5 cm2 while the BMP isolate showed no inhibition zone. In addition, this study also evaluated the virulence reduction of R. solanacearum due to the treatment of Streptomyces spp. The virulence reduction test showed that TMP isolates were able to reduce virulence by 70% while BMP isolates were able to reduce the virulence of R. solanacearum by 4%. These findings provide new insights into the development of R. solanacearum control strategies. In conclusion, isolates of Streptomyces sp. especially BMP showed potential in reducing the virulence of R. solanacearum, thus providing new hope in efforts to increase chili production sustainably.

Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi Amalia, Anisa Rizki; Purnawati, Arika; Prasetyawati, Endang Triwahyu; Yanti, Vika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4822

The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.

Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.

Resistance response of five maize grain varieties to Sitophilus zeamais and Sitophilus oryzae (Coleoptera: Curculionidae) Octavianti, Dewi Fortuna; Megasari, Dita; Prasetyawati, Endang Triwahyu
Agrovigor Vol 17, No 2 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i2.26407

Warehouse pests that have the potential to damage corn kernels are Sitophilus zeamais and Sitophilus oryzae. Information on the resistance of corn kernels of several varieties is still little reported. Research on the resistance response of five varieties of corn kernels (Madura-3, Lokal Madura, Pioneer P32 Singa, P27 Gajah, and NK 212) to S. zeamais and S. oryzae pests was conducted at the Plant Health Laboratory, Agrotechnology Study Program, Faculty of Agriculture, National Development University “Veteran” East Java. This study aims to determine the response of feed type resistance in the form of five varieties of corn seeds to the attack of S. zeamais and S. oryzae pests. The results showed that the highest percentage of mortality of S. zeamais and S. oryzae imago was in corn seeds of Madura-3 (17.15%) and NK 212 (37.22%) varieties. Fecundity tests on S. zeamais and S. oryzae pests with the lowest mean were on corn seeds of P27 Gajah (6.33 grains) and NK 212 (4.33 grains). The lowest percentage of seed weight loss in S. zeamais and S. oryzae pests was in the varieties P27 Gajah (1.34%) and NK 212 (1.05%). The highest percentage of sex ratio of S. zeamais and S. oryzae imago was in the varieties P27 Gajah (1.57%) and NK 212 (1.23%). The conclusion of this study is that the maize seeds that are resistant to S. zeamais attack are the P27 Gajah variety, while the maize seeds that are resistant to S. oryzae attack are the NK 212 variety.

Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.

Potensi Trichoderma sp. Asal Tanah Gambut Sebagai Agensia Pengendali Biologi Jamur Patogen Terbawa Benih Padi Maulina Aizah, Shinta; Purnawati, Arika; Prasetyawati, Endang Triwahyu
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15354

Background: Rice (Oryza sativa) is the world's second most important food crop after wheat. In Indonesia, more than 90% of the population consumes rice, and there is a very high level of dependence, so rice has essential value for the Indonesian population. However, this has not been accompanied by the availability of healthy seeds because many pathogenic fungi are carried by rice seeds, such as Rhizopus sp., Mucor sp., Aspergillus sp., Fusarium sp., Curvularia sp. Seed treatment using synthetic fungicides is an effort to control pathogenic fungi on seeds, but it hurts seeds, humans, and the environment. A safe control alternative is using biological agents, namely the fungus Trichoderma sp. isolated from peatlands and has an antibiosis mechanism. The research aimed to test the potential of the fungus Trichoderma sp. from peat soil as a biological control agent for pathogenic fungi carried by rice seeds (Oryza sativa). Methods: The test uses the incubation method on PDA media and the growing test method on sterile soil media. The research used a completely randomized design with a factor of 2 isolates of Trichoderma sp. and eight replications and data analysis using BNJ5%. Results: isolation results obtained two isolates of Trichoderma sp. (P1 and P2), two isolates of Trichoderma sp. (P1 and P2) were able to suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and the growing on test method, 3.75%, two isolates of Trichoderma sp. able to increase the germination capacity of rice seeds by P1 (12.50%) and P2 (31.25%) respectively. Conclusions: Two isolates of Trichoderma sp. (P1 and P2) could suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and in the growing on test method. Apart from that, two isolates of Trichoderma sp.

UJI EFEKTIVITAS MINYAK ATSIRI SERAI WANGI (Cymbopogon nardus L.) DALAM MENGENDALIKAN JAMUR (Alternaria porri) PENYEBAB PENYAKIT BERCAK UNGU PADA TANAMAN BAWANG PREI (Allium Fistulosum L.) Abifah, Serlia Nur; Prasetyawati, Endang Triwahyu; Suryaminarsih, Penta
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8781

The growth and production of oil palm is greatly influenced by the presence of Plant Pest Organisms (OPT), one of which is purple spot disease caused by the fungus Alternia porri. Control efforts using vegetable fungicides, namely citronella essential oil. The aim of this research is to determine the concentration of citronella essential oil as a vegetable fungicide that is effective in suppressing the growth of the Alternaria porri fungus in vitro. This research was carried out from October-December 2023. The location of this research was carried out at the Plant Health Laboratory, Faculty of Agriculture, National Development University "Veteran" East Java. The research design used was a Completely Randomized Design (CRD) using a single factor of citronella essential oil concentration, namely 0%, 10%, 20%, 30% and 40%. Observation variables include the inhibition test. The data from the research was carried out using Analysis of Varience (ANOVA) and continued with a multiple comparison test using the BNJ advanced test at a real level of 5% using SPPS software version 21. The results of the research showed that the largest percentage of inhibitory power on the last day of observation was obtained at a concentration of 40%, namely amounting to 32.92% and 30% concentration, namely 20.29%, while the lowest inhibitory power was obtained from the 20% concentration treatment, namely 7.74% and 10% concentration, namely 8.89%. The higher the concentration of citronella essential oil used, the stronger the inhibition of fungal growth will be.Keywords : essential oils, citronella, concentrate, Alternia porri

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