Garuda - Garba Rujukan Digital

Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi Amalia, Anisa Rizki; Purnawati, Arika; Prasetyawati, Endang Triwahyu; Yanti, Vika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4822

The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.

Resistance response of five maize grain varieties to Sitophilus zeamais and Sitophilus oryzae (Coleoptera: Curculionidae) Octavianti, Dewi Fortuna; Megasari, Dita; Prasetyawati, Endang Triwahyu
Agrovigor Vol 17, No 2 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i2.26407

Warehouse pests that have the potential to damage corn kernels are Sitophilus zeamais and Sitophilus oryzae. Information on the resistance of corn kernels of several varieties is still little reported. Research on the resistance response of five varieties of corn kernels (Madura-3, Lokal Madura, Pioneer P32 Singa, P27 Gajah, and NK 212) to S. zeamais and S. oryzae pests was conducted at the Plant Health Laboratory, Agrotechnology Study Program, Faculty of Agriculture, National Development University “Veteran” East Java. This study aims to determine the response of feed type resistance in the form of five varieties of corn seeds to the attack of S. zeamais and S. oryzae pests. The results showed that the highest percentage of mortality of S. zeamais and S. oryzae imago was in corn seeds of Madura-3 (17.15%) and NK 212 (37.22%) varieties. Fecundity tests on S. zeamais and S. oryzae pests with the lowest mean were on corn seeds of P27 Gajah (6.33 grains) and NK 212 (4.33 grains). The lowest percentage of seed weight loss in S. zeamais and S. oryzae pests was in the varieties P27 Gajah (1.34%) and NK 212 (1.05%). The highest percentage of sex ratio of S. zeamais and S. oryzae imago was in the varieties P27 Gajah (1.57%) and NK 212 (1.23%). The conclusion of this study is that the maize seeds that are resistant to S. zeamais attack are the P27 Gajah variety, while the maize seeds that are resistant to S. oryzae attack are the NK 212 variety.

Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.

Potensi Trichoderma sp. Asal Tanah Gambut Sebagai Agensia Pengendali Biologi Jamur Patogen Terbawa Benih Padi Maulina Aizah, Shinta; Purnawati, Arika; Prasetyawati, Endang Triwahyu
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15354

Background: Rice (Oryza sativa) is the world's second most important food crop after wheat. In Indonesia, more than 90% of the population consumes rice, and there is a very high level of dependence, so rice has essential value for the Indonesian population. However, this has not been accompanied by the availability of healthy seeds because many pathogenic fungi are carried by rice seeds, such as Rhizopus sp., Mucor sp., Aspergillus sp., Fusarium sp., Curvularia sp. Seed treatment using synthetic fungicides is an effort to control pathogenic fungi on seeds, but it hurts seeds, humans, and the environment. A safe control alternative is using biological agents, namely the fungus Trichoderma sp. isolated from peatlands and has an antibiosis mechanism. The research aimed to test the potential of the fungus Trichoderma sp. from peat soil as a biological control agent for pathogenic fungi carried by rice seeds (Oryza sativa). Methods: The test uses the incubation method on PDA media and the growing test method on sterile soil media. The research used a completely randomized design with a factor of 2 isolates of Trichoderma sp. and eight replications and data analysis using BNJ5%. Results: isolation results obtained two isolates of Trichoderma sp. (P1 and P2), two isolates of Trichoderma sp. (P1 and P2) were able to suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and the growing on test method, 3.75%, two isolates of Trichoderma sp. able to increase the germination capacity of rice seeds by P1 (12.50%) and P2 (31.25%) respectively. Conclusions: Two isolates of Trichoderma sp. (P1 and P2) could suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and in the growing on test method. Apart from that, two isolates of Trichoderma sp.

Isolation and Characterization of Endophytic Bacteria from The Roots of Avicennia sp. in The Mangrove Area of Gunung Anyar, Surabaya Azzahra, Fatimah; Prasetyawati, Endang Triwahyu; Lestari, Safira Rizka
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6596

Mangroves are unique ecosystems that support biodiversity, including endophytic microorganisms. Endophytic bacteria group is a group of microbes that have a mutualistic symbiosis to the host plant without harming the host, and can act as biocontrol agents and plant stimulants. Endophytic bacteria have a very wide host range. Each plant has an abundance and diversity of endophytic bacteria, depending on the internal and external factors that affect it. This study aims to determine the characteristics of endophytic bacteria and identify the diversity of endophytic bacteria found in Avicennia sp. Method Sampling from mangrove area of Gunung Anyar, Surabaya. Characterization of endophytic bacteria was done by Gram test, spore staining test, catalase test, oxidative-fermentative test, and selective media test (King's B and Yeast Dextrose Carbonate). The results showed the existence of several isolates of endophytic bacteria that have morphological diversity and different characteristics, such as shape, color, colony edge, and colony surface. Conclusions In this study has been successfully isolated some endophytic bacteria such as Lactobacillus sp., Corynebacterium sp., Bacillus sp., Xanthomonas sp., and there are also endophytic bacterial isolates that have not been identified. This study contributes to an early understanding of the diversity of microorganisms in mangrove ecosystems and opens up opportunities for further exploration of their potential applications, one of which is in agriculture

UJI EFEKTIVITAS MINYAK ATSIRI SERAI WANGI (Cymbopogon nardus L.) DALAM MENGENDALIKAN JAMUR (Alternaria porri) PENYEBAB PENYAKIT BERCAK UNGU PADA TANAMAN BAWANG PREI (Allium Fistulosum L.) Abifah, Serlia Nur; Prasetyawati, Endang Triwahyu; Suryaminarsih, Penta
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8781

The growth and production of oil palm is greatly influenced by the presence of Plant Pest Organisms (OPT), one of which is purple spot disease caused by the fungus Alternia porri. Control efforts using vegetable fungicides, namely citronella essential oil. The aim of this research is to determine the concentration of citronella essential oil as a vegetable fungicide that is effective in suppressing the growth of the Alternaria porri fungus in vitro. This research was carried out from October-December 2023. The location of this research was carried out at the Plant Health Laboratory, Faculty of Agriculture, National Development University "Veteran" East Java. The research design used was a Completely Randomized Design (CRD) using a single factor of citronella essential oil concentration, namely 0%, 10%, 20%, 30% and 40%. Observation variables include the inhibition test. The data from the research was carried out using Analysis of Varience (ANOVA) and continued with a multiple comparison test using the BNJ advanced test at a real level of 5% using SPPS software version 21. The results of the research showed that the largest percentage of inhibitory power on the last day of observation was obtained at a concentration of 40%, namely amounting to 32.92% and 30% concentration, namely 20.29%, while the lowest inhibitory power was obtained from the 20% concentration treatment, namely 7.74% and 10% concentration, namely 8.89%. The higher the concentration of citronella essential oil used, the stronger the inhibition of fungal growth will be.Keywords : essential oils, citronella, concentrate, Alternia porri

Eksplorasi dan Uji Antagonis Bakteri Endofit Dari Daun Tanaman Mangrove Avicennia sp. Terhadap Jamur Patogen Alteria sp. Secara In Vitro Perwira Moekti, Nirwana Septania Galih; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.30972

Mangrove (Avicennia sp.) merupakan salah satu ekosistem penting yang sering ditemukan di muara sungai yang memiliki kemampuan untuk tumbuh dan berkembang di zona salinitas tinggi. Salah satu jenis tanaman mangrove yang bermanfaat dan terdapat berbagai jenis bakteri endofit adalah Avicennia marina. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang berpotensi sebagai agensia hayati pada daun tanaman bakau Avicennia sp. terhadap cendawan patogen Alternaria sp. Metode isolasi yang digunakan adalah penanaman langsung. Pengujian antagonis dilakukan dengan metode dual culture melalui pengukuran diameter zona hambat. Sampel yang diambil adalah daun yang sehat dan segar. Bakteri endofit yang diperoleh sebanyak 24 isolat bakteri yang memiliki morfologi yang berbeda. Diameter zona hambat dengan kategori kuat adalah isolat D28 sebesar 12,5 mm dengan kategori kuat dan kategori sedang adalah isolat D13, D19 sebesar 7,5 mm; 8,5 mm. Mekanisme kerja bakteri endofit adalah antibiosis dan kompetisi ruang. Hasil penelitian ini dapat disimpulkan bahwa terdapat bakteri endofit dari daun mangrove Avicennia sp. yang berpotensi sebagai antijamur terhadap jamur patogen Alternaria sp.

Antagonistic Test of Bacillus spp. against Fusarium sp., the Causal Agent of Wilt Disease of Red Chili Plants Heriyati, Sukma; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.45386

Wilt disease caused by Fusarium oxysporum is one of the diseases affecting chili plants. The utilization of Bacillus spp. can be a solution for plant disease control because it can colonize plants and produce useful microbe compounds to inhibit the development of plant pathogen. This study aimed to determine the ability of Bacillus spp. to suppress the growth of Fusarium sp. isolates from chili plants in vitro. Bacillus sp. isolates Ba-6, Ba-9, Ba-12, Ba-15, Ba-17, and a control (aquadest) were used as treatments, each replicated four times. The observation parameters were the inhibition zone test and microscopic observations of the morphology of Fusarium sp. after the inhibition zone test. The highest inhibition was shown in the treatment of Bacillus sp. isolate Ba-15, which was 20.02%. The observation of Fusarium sp. hyphae after the inhibition zone test showed abnormal growth, which was different for each isolate. Some hyphae were bent, coiled, shrunken, swollen, curled, or lysed.

Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search