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Eksplorasi dan Uji Antagonis Bakteri Endofit Dari Daun Tanaman Mangrove Avicennia sp. Terhadap Jamur Patogen Alteria sp. Secara In Vitro Perwira Moekti, Nirwana Septania Galih; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.30972

Mangrove (Avicennia sp.) merupakan salah satu ekosistem penting yang sering ditemukan di muara sungai yang memiliki kemampuan untuk tumbuh dan berkembang di zona salinitas tinggi. Salah satu jenis tanaman mangrove yang bermanfaat dan terdapat berbagai jenis bakteri endofit adalah Avicennia marina. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang berpotensi sebagai agensia hayati pada daun tanaman bakau Avicennia sp. terhadap cendawan patogen Alternaria sp. Metode isolasi yang digunakan adalah penanaman langsung. Pengujian antagonis dilakukan dengan metode dual culture melalui pengukuran diameter zona hambat. Sampel yang diambil adalah daun yang sehat dan segar. Bakteri endofit yang diperoleh sebanyak 24 isolat bakteri yang memiliki morfologi yang berbeda. Diameter zona hambat dengan kategori kuat adalah isolat D28 sebesar 12,5 mm dengan kategori kuat dan kategori sedang adalah isolat D13, D19 sebesar 7,5 mm; 8,5 mm. Mekanisme kerja bakteri endofit adalah antibiosis dan kompetisi ruang. Hasil penelitian ini dapat disimpulkan bahwa terdapat bakteri endofit dari daun mangrove Avicennia sp. yang berpotensi sebagai antijamur terhadap jamur patogen Alternaria sp.

The Potential of Bacillus spp. in Suppressing Colletotrichum capsici that Causes Antracnose in Red Chili Rani, Sasiska; Endang Triwahyu Prasetyawati; Herry Nirwanto; Sri Wiyatiningsih
International Journal of Applied Biology Vol. 7 No. 1 (2023): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Red Chili (Capsicum annum L.) is a horticultural plants that is classified as an important commodity and has high economic value. One of the main problems in the production of red chili is anthracnose disease caused by the Colletotrichum capsici pathogen. Bacillus spp. expected to have the potential to inhibit the growth of C. capsici and stimulate the growth of plant. This study aims to determine the potential of Bacillus spp., in suppressing the growth of the C. capsici fungus and stimulating the growth of red chili. This study used a Completely Randomized Design (CRD) with the treatment without Bacillus spp., chemical fungicides, Bacillus sp. (Ba-6), Bacillus sp. (Ba-9), Bacillus sp. (Ba-12), Bacillus sp. (Ba-15), and Bacillus sp. (Ba-17). The treatment was repeated 4 times and the treatment unit contained 5 red chili/polybag. The results showed that the highest suppression of C. capsici in the treatment of Bacillus sp. Ba-15 was 6.25% compared to the negative control. The best bacteria that can stimulate the growth of red chili is Bacillus sp. Ba-9 with an average plant height 20.10 cm and an average number of leaves 10.49

Antagonistic Test of Bacillus spp. against Fusarium sp., the Causal Agent of Wilt Disease of Red Chili Plants Heriyati, Sukma; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.45386

Wilt disease caused by Fusarium oxysporum is one of the diseases affecting chili plants. The utilization of Bacillus spp. can be a solution for plant disease control because it can colonize plants and produce useful microbe compounds to inhibit the development of plant pathogen. This study aimed to determine the ability of Bacillus spp. to suppress the growth of Fusarium sp. isolates from chili plants in vitro. Bacillus sp. isolates Ba-6, Ba-9, Ba-12, Ba-15, Ba-17, and a control (aquadest) were used as treatments, each replicated four times. The observation parameters were the inhibition zone test and microscopic observations of the morphology of Fusarium sp. after the inhibition zone test. The highest inhibition was shown in the treatment of Bacillus sp. isolate Ba-15, which was 20.02%. The observation of Fusarium sp. hyphae after the inhibition zone test showed abnormal growth, which was different for each isolate. Some hyphae were bent, coiled, shrunken, swollen, curled, or lysed.

THE POTENTIAL OF Zinnia elegans AND Bacillus sp. as Lead (Pb) BIOREMEDIATION AGENTS Febiola, Regina Rosari; Prasetyawati, Endang Triwahyu; Nirwanto , Herry
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13482

Lead (Pb) pollution in soil is a serious problem that reduces crop productivity. One approach to address this issue is through bioremediation and Phytoremediation, using microorganism and plants that can tolerate and bind heavy metals. This study examine the potential of Zinnia elegans (Jacq.) and Bacillus sp., and in combination to reduce Pb in soil. The experiment lasted 60 days using a completely randomized design with six treatments: P0 (control, Pb-contaminated soil without plants or bacteria), P1 (Z. elegans only), P2 (Bacillus sp. only), P3 (combination), P4 (non-contaminated soil with Z. elegans and Bacillus sp.), and P5 (non-contaminated soil with Z. elegans only). The results showed that treatment P3 reduced Pb concentration by 62.31 ppm, while no significant differences were observed among treatments in terms of plant growth parameters. Combining Z. elegans with Bacillus sp. may serve as a practical approach to enhance Pb remediation in contaminated soils.

THE POTENTIAL OF Zinnia elegans AND Bacillus sp. as Lead (Pb) BIOREMEDIATION AGENTS Febiola, Regina Rosari; Prasetyawati, Endang Triwahyu; Nirwanto , Herry
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13482

Lead (Pb) pollution in soil is a serious problem that reduces crop productivity. One approach to address this issue is through bioremediation and Phytoremediation, using microorganism and plants that can tolerate and bind heavy metals. This study examine the potential of Zinnia elegans (Jacq.) and Bacillus sp., and in combination to reduce Pb in soil. The experiment lasted 60 days using a completely randomized design with six treatments: P0 (control, Pb-contaminated soil without plants or bacteria), P1 (Z. elegans only), P2 (Bacillus sp. only), P3 (combination), P4 (non-contaminated soil with Z. elegans and Bacillus sp.), and P5 (non-contaminated soil with Z. elegans only). The results showed that treatment P3 reduced Pb concentration by 62.31 ppm, while no significant differences were observed among treatments in terms of plant growth parameters. Combining Z. elegans with Bacillus sp. may serve as a practical approach to enhance Pb remediation in contaminated soils.

Eficacy of Paenibacillus sp. Using seed treatment for controlling Bacterial Leaf Blight Caused by Xanthomonas sp. in Rice Plants Al Rahmad, Nirmala Putri; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65040

Rice (Oryza sativa L.) is one of the important food commodities consumed by the Indonesian people. One of the reasons for the decline in rice production is infection by the pathogen Xanthomonas oryzae pv. oryzae (Xoo) which causes bacterial leaf blight (BLB) and it’s seed-borne pathogen. The purpose of the experiment is to to evaluate the effectiveness of seed soaking using Paenibacillus sp. to control BLB in rice plants. Efforts to control BLB and improve rice plant growth were carried out by seed treatment using biocontrol agent has potential as biological control for BLB in rice plants by producing antibiotic. This experiment used a Completely Randomized Design with 4 treatments (control, Paenibacillus sp., Bacillus sp. BTH22, Paenibacillus polymyxa) and 5 replications. Data analysis was performed using analysis of variance (ANOVA) at a 5% significance level using IBM SPSS Statistics version 22 software. If the analysis results showed significant differences between treatments, an Honestly Significant Difference (HSD) test was performed at the 5% level. The research results showed that seed soaking using Paenibacillus sp. was able to reduce the incubation period of HDB disease by up to 11 days, with the lowest disease attack intensity being 10.21%, the highest seed germination rate being 91.25%, and an efficacy level of 53.32%.

Consortium of Bacillus sp. BTH22 and Trichoderma sp. to Control Bacterial Leaf Blight Disease in Rice Plants Dwirizki Permata, Aprilla Hendiana; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65958

Rice is a major food commodity in Indonesia that is currently experiencing a decline in production due to several factors, one of which is bacterial leaf blight (BLB) caused by the bacterium Xanthomonas sp. The use of chemicals in controlling this disease has many limitations, so alternatives are needed, such as antagonistic microbes. This study aims to evaluate the effectiveness of Bacillus sp. BTH22 and Trichoderma sp. individually and in consortium in suppressing BLB disease in rice plants in vitro and in vivo. The study was conducted at the Plant Health Laboratory of Universitas Pembangunan Nasional “Veteran” Jawa Timur and Green House of Kebun Bibit Wonorejo from March to June 2025. The methods used included in vitro diffusion well test and in vivo disease intensity observation with a completely randomized design consisting of 5 treatments, namely control and spraying of antagonistic microbes Bacillus sp. BTH22 and Trichoderma sp. alone or in consortium with 4 replicates. Data analysis used analysis of variance (ANOVA) and post-hoc HSD tests at a 5% significance level. The results show that the treatment with the Bacillus sp. BTH22 and Trichoderma sp. consortium was the most effective, with an inhibition zone of 13 mm, which is classified as strong, and the lowest disease intensity of 30.02% at 55 HST with a suppression rate of 69.98%. These results indicate that the consortium of the two antagonistic microorganisms has high potential as an effective and sustainable biological control agent for BLB based on the observed parameters of inhibition zone and disease attack intensity.

Isolation and Identification of Indigenous Bacteria Potential to Degradate Mancozeb from Shallots (Allium cepa L.) Cultivation Land in Nganjuk District Khairunnisa, Nabila Zahra; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67683

The use of a fungicide containing the active ingredient mancozeb in shallot fields to control moler disease, if not managed in terms of dose and frequency, it has the potential to harm soil in physical, chemical and biological ways. One method for repairing this damage is too use local microbes. This research attempts to determine the types of bacteria in soil treated to mancozeb fungicide through a morphological and physiological characterization process. Soil samples were collected from the shallot producing region of Balongrejo District, Nganjuk Regency. The method in this research is to isolate soil samples at a depth of 0-20 cm. Bacteria were isolated and multiplied using the streak plate technique on nutrient agar medium. Identification was performed on macroscopic and microscopic morphological features well as physiological characters based on the method of Schaad et al. (2001). Bergey's Manual of Determinative Bacteriology Ninth Edition was then used to identify the isolated bacteria. The research results showed that 14 bacterial isolates were isolated, 5 isolates suspected to be the genus Bacillus, 7 isolates suspected to be the genus Corynebacterium, 1 isolate suspected to be Lactobacillus, and 1 isolate unidentified. All indigenous bacterial isolates were able to reduce the concentration of mancozeb fungicide through measurements using a UV-Vis spechtrophotometer with a wavelength of 268 nm. The highest degradation percentage was bacterial isolate I8 (75%) and the lowest was bacterial isolate I7 (19%).

EFEKTIVITAS LIMBAH CAIR TAHU DAN AIR KELAPA SEBAGAI MEDIA ALTERNATIF PERBANYAKAN Bacillus mycoides DALAM MENGENDALIKAN ANTRAKNOSA CABAI SECARA IN VITRO Wafiah Huwaina Zata Dini; Endang Triwahyu Prasetyawati; Arika Purnawati
Agrika Vol. 20 No. 1 (2026): MEI 2026
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v20i1.7638

Cabai (Capsicum annuum L.) merupakan komoditas hortikultura utama di Indonesia. Organisme pengganggu tanaman (OPT) yang menyerang cabai adalah Colletotrichum sp. penyebab penyakit antraknosa. Bacillus mycoides dapat mengendalikan penyakit antraknosa. Limbah cair tahu dan air kelapa dapat dimanfaatkan sebagai media alternatif perbanyakan B. mycoides. Penelitian disusun dengan rancangan acak lengkap faktorial (RALF) dengan dua faktor dan tiga ulangan. Faktor pertama adalah media alternatif: NB/PDA, limbah cair tahu, air kelapa, dan limbah cair tahu + air kelapa. Faktor kedua adalah umur biakan: 24 jam dan 48 jam. Data dianalisis menggunakan Analisa ragam (Anova) dan apabila terdapat pengaruh yang nyata, maka dilanjutkan dengan uji BNT pada taraf 5%. Hasil penelitian menunjukkan bahwa terdapat interaksi perlakuan yang nyata antara perlakuan jenis media dengan umur biakan terhadap pertumbuhan Bacillus mycoides serta antagonismenya terhadap jamur Colletotrichum sp. Perlakuan B3A1 (media air kelapa dengan umur biakan 24 jam) menghasilkan jumlah koloni sebesar 1.53 × CFU/ml dan mengakibatkan daya hambat tertinggi terhadap Colletotrichum sp. sebesar 40.48%.

Potency of the Consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides Isolates Against Bacterial Wilt Disease In-Vitro Yosua Nathanael Itona Sagala; Endang Triwahyu Prasetyawati; Yenny Wuryandari
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.

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