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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Cropsaver : Journal of Plant Protection Agrovigor: Jurnal Agroekoteknologi Jurnal Agroekoteknologi BIOEDUSCIENCE JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Prasetyawati, Endang Triwahyu
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Veterinary

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THE POTENTIAL OF Zinnia elegans AND Bacillus sp. as Lead (Pb) BIOREMEDIATION AGENTS Febiola, Regina Rosari; Prasetyawati, Endang Triwahyu; Nirwanto , Herry
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13482

Abstract

Lead (Pb) pollution in soil is a serious problem that reduces crop productivity. One approach to address this issue is through bioremediation and Phytoremediation, using microorganism and plants that can tolerate and bind heavy metals. This study examine the potential of Zinnia elegans (Jacq.) and Bacillus sp., and in combination to reduce Pb in soil. The experiment lasted 60 days using a completely randomized design with six treatments: P0 (control, Pb-contaminated soil without plants or bacteria), P1 (Z. elegans only), P2 (Bacillus sp. only), P3 (combination), P4 (non-contaminated soil with Z. elegans and Bacillus sp.), and P5 (non-contaminated soil with Z. elegans only). The results showed that treatment P3 reduced Pb concentration by 62.31 ppm, while no significant differences were observed among treatments in terms of plant growth parameters. Combining Z. elegans with Bacillus sp. may serve as a practical approach to enhance Pb remediation in contaminated soils.
Eficacy of Paenibacillus sp. Using seed treatment for controlling Bacterial Leaf Blight Caused by Xanthomonas sp. in Rice Plants Al Rahmad, Nirmala Putri; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65040

Abstract

Rice (Oryza sativa L.) is one of the important food commodities consumed by the Indonesian people. One of the reasons for the decline in rice production is infection by the pathogen Xanthomonas oryzae pv. oryzae (Xoo) which causes bacterial leaf blight (BLB) and it’s seed-borne pathogen. The purpose of the experiment is to to evaluate the effectiveness of seed soaking using Paenibacillus sp. to control BLB in rice plants. Efforts to control BLB and improve rice plant growth were carried out by seed treatment using biocontrol agent has potential as biological control for BLB in rice plants by producing antibiotic. This experiment used a Completely Randomized Design with 4 treatments (control, Paenibacillus sp., Bacillus sp. BTH22, Paenibacillus polymyxa) and 5 replications. Data analysis was performed using analysis of variance (ANOVA) at a 5% significance level using IBM SPSS Statistics version 22 software. If the analysis results showed significant differences between treatments, an Honestly Significant Difference (HSD) test was performed at the 5% level. The research results showed that seed soaking using Paenibacillus sp. was able to reduce the incubation period of HDB disease by up to 11 days, with the lowest disease attack intensity being 10.21%, the highest seed germination rate being 91.25%, and an efficacy level of 53.32%.
Consortium of Bacillus sp. BTH22 and Trichoderma sp. to Control Bacterial Leaf Blight Disease in Rice Plants Dwirizki Permata, Aprilla Hendiana; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65958

Abstract

Rice is a major food commodity in Indonesia that is currently experiencing a decline in production due to several factors, one of which is bacterial leaf blight (BLB) caused by the bacterium Xanthomonas sp. The use of chemicals in controlling this disease has many limitations, so alternatives are needed, such as antagonistic microbes. This study aims to evaluate the effectiveness of Bacillus sp. BTH22 and Trichoderma sp. individually and in consortium in suppressing BLB disease in rice plants in vitro and in vivo. The study was conducted at the Plant Health Laboratory of  Universitas Pembangunan Nasional “Veteran” Jawa Timur and Green House of Kebun Bibit Wonorejo from March to June 2025. The methods used included in vitro diffusion well test and in vivo disease intensity observation with a completely randomized design consisting of 5 treatments, namely control and spraying of antagonistic microbes Bacillus sp. BTH22 and Trichoderma sp. alone or in consortium with 4 replicates. Data analysis used analysis of variance (ANOVA) and post-hoc HSD tests at a 5% significance level. The results show that the treatment with the Bacillus sp. BTH22 and Trichoderma sp. consortium was the most effective, with an inhibition zone of 13 mm, which is classified as strong, and the lowest disease intensity of 30.02% at 55 HST with a suppression rate of 69.98%. These results indicate that the consortium of the two antagonistic microorganisms has high potential as an effective and sustainable biological control agent for BLB based on the observed parameters of inhibition zone and disease attack intensity.
Isolation and Identification of Indigenous Bacteria Potential to Degradate Mancozeb from Shallots (Allium cepa L.) Cultivation Land in Nganjuk District Khairunnisa, Nabila Zahra; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67683

Abstract

The use of a fungicide containing the active ingredient mancozeb in shallot fields to control moler disease, if not managed in terms of dose and frequency, it has the potential to harm soil in physical, chemical and biological ways. One method for repairing this damage is too use local microbes. This research attempts to determine the types of bacteria in soil treated to mancozeb fungicide through a morphological and physiological characterization process. Soil samples were collected from the shallot producing region of Balongrejo District, Nganjuk Regency. The method in this research is to isolate soil samples at a depth of 0-20 cm. Bacteria were isolated and multiplied using the streak plate technique on nutrient agar medium. Identification was performed on macroscopic and microscopic morphological features well as physiological characters based on the method of Schaad et al. (2001). Bergey's Manual of Determinative Bacteriology Ninth Edition was then used to identify the isolated bacteria. The research results showed that 14 bacterial isolates were isolated, 5 isolates suspected to be the genus Bacillus, 7 isolates suspected to be the genus Corynebacterium, 1 isolate suspected to be Lactobacillus, and 1 isolate unidentified. All indigenous bacterial isolates were able to reduce the concentration of mancozeb fungicide through measurements using a UV-Vis spechtrophotometer with a wavelength of 268 nm. The highest degradation percentage was bacterial isolate I8 (75%) and the lowest was bacterial isolate I7 (19%).
ALGINATE-SEED ENCAPSULATION CONTAINING ENDOPHYTIC Bacillus cereus BTH21 FOR BIOCONTROLLING WILT DISEASE IN EGGPLANT Purnawati, Arika; Prasetyawati, Endang Triwahyu; Aditya, Haidar Fari; Saefur Rohman
BIOTROPIA Vol. 32 No. 3 (2025): BIOTROPIA Vol. 32 No. 3 December 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.3.2410

Abstract

HIGHLIGHTSSeed encapsulation using alginate and B. cereus BTH21 for biocontrol of wilt disease in eggplant.ABSTRACTBacterial wilt is a major plant disease caused by Ralstonia solanacearum that infects important crops, such as eggplant, causing wilt, stunted growth, and reduced yield. The biocontrol of the pathogen can be done by employing antagonistic bacteria, yet direct inoculation into the soils are often ineffective due to low population of the pathogen in the soils, which also easily washed away by watering activity. Seed encapsulation technique, using alginate, zeolite and peat, containing antagonistic bacteria Bacillus cereus BTH21 to control R. solanacearum is the novelty proposed in this research. Hence, this research aimed to develop alginate seed-encapsulation technique containing Bacillus cells as a mean to biologically control R. solanacearum in eggplant. Endophytic Bacillus cereus BTH21 strains was isolated from healthy eggplant tissue obtained from Kediri, East Java Province, Indonesia. Subsequent isolation, purification and molecular identification confirmed the identity of the strain. Three seed-encapsulation formulations were made: a) control (no encapsulation); b) alginate + zeolite + Bacillus (Al+Z+B); and c) alginate + peat + Bacillus (Al+P+B). A field experiment was designed in a Completely Randomized Design (CRD) where the alginate-encapsulated eggplant seeds were sown along with deliberate R. solanacearum inoculation (107 cfu/mL) into the soils. Observations were carried out every 7 days from 0 – 49 days after sowing (DAS) on several parameters, i.e., incubation period, infection rate, germination percentage, plant height, and number of leaves. The data were analyzed by using analysis of variance (ANOVA) followed by Duncan’s multiple range test at a significance level of P < 0.05. The results showed that both seed encapsulation formulations prolonged incubation period (15 and 13 DAS) compared to the control (10 DAS), reduced infection rate, increased germination rate, as well as higher agronomic performances (plant height, number of leaves) compared to the control at 7 – 49 DAS. Overall, this results indicated the potential of alginate seed-encapsulation techniques containing biocontrol agents to control plant diseases.
Co-Authors Abifah, Serlia Nur Aditya, Haidar Fari Al Rahmad, Nirmala Putri Amalia, Anisa Rizki Arika Purnawati Dita Megasari Dwirizki Permata, Aprilla Hendiana Fatimah Azzahra Febiola, Regina Rosari Heriyati, Sukma Khairunnisa, Nabila Zahra Lestari, Safira Rizka Maulina Aizah, Shinta Nirwanto , Herry Octavianti, Dewi Fortuna Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Saefur Rohman Sagala, Yosua Nathanael Itona Yanti, Vika Yenny WURYANDARI