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PERBAIKAN GAMBARAN LESI ATEROSKLEROTIK DENGAN PEMBERIAN EKSTRAK BETA VULGARIS PADA TIKUS MODEL DIET ATHEROGENIK Hardimarta, Fransisca Pramesshinta; Ikawati, Kartika; Yuniarti, Christina Ary
Media Farmasi Indonesia Vol. 15 No. 1 (2020): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.432 KB) | DOI: 10.53359/mfi.v15i1.140

Abstract

Atherosclerosis is characterized by the accumulation of fat in the arteries so it forms plaque that causes thickening of the intima and blockage of the arteries. Beet roots are rich of phytochemical compounds that have antioxidant activity. Design of research is a laboratory experimental study with a Post Test Only Randomized Control Group Design. The population of this study was male Wistar rats with age 2-3 months and body weight 125-200 grams. The study sample was randomly selected and consists of 7 groups and 5 rats for each group. The average number of foam cells in the baseline group is 17.6; negative control group is 65.8; positive control is 54.6; group I is 38.8; group II is 27.4; group III is 46.4; and group IV is 42.4. The average thickness of the aortic intima tunica in the baseline group is 136.8 µ; negative control group is 251.4 µ; positive control is 211.8 µ; group I is 202.8 µ; group II is 184 µ; group III is 242.8 µ; and group IV is 210.6 µ. Statistical test results showed that there were significant differences in supplementation of beet root extracts against aortic thickness and the number of foam cells in rats fed atherogenic diets.
PERBEDAAN HITUNG JUMLAH TROMBOSIT CARA LANGSUNG DAN BARBARA BROWN (SADT ZONA IV,V,VI dan ZONA V) : (SADT ZONA IV,V,VI dan ZONA V) Kartika Ikawati Ikawati
Journal of Nursing and Health Vol. 9 No. 3 (2024): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v9i3.379

Abstract

Direct and Barbara Brown manual platelet counts are the reference methods recommended by ICSH. This manual method is used to confirm if the automatic method can not produce accurate results. Until now there has been no reference that mentions the SADT reading zone for estimating platelet counts. Peripheral blood smear has six zones with different cell distribution and density. The aim of this study was to determine the difference in platelet counts examined using the direct method, Barbara Brown's method of reading zones IV, V, VI and Barbara Brown's reading of zone V. This type of research is analytical observation with a cross-sectional design. The research sample was 30 taken using random sampling technique from the student population of AKKES 17 Agustus  1945 Semarang. The research was conducted in March 2024 at the AKKES Hematology Lab 17 Agustus 1945 Semarang. Counting the number of platelets is done directly by diluting the blood with Rees Ecker's solution into a thoma pipette. The estimation method for calculating the number of platelets (Barbara Brown) is done by calculating the average number of platelets in 10 SADT fields of view and multiplying by a conversion factor of 20.000. . The highest platelet count results were obtained from the Barbara Brown method (SADT zone IV, V, VI) with a mean of 342,470 ± 46,185 cells/(µL), followed by the direct method with a mean of 327,230 ± 50,447 cells/µL and the lowest was the SADT zone V a mean of 319,930 ± 39,172 cells/µL. There was a smaller difference between the direct method and Barabara Brown (SADT zone V). The results of the Anova and Post Hoc tests showed p > 0.05, which means there was no significant difference between the three methods. Keywords: Platelet count; Direct method; Barbara Brown
HUBUNGAN VOLUME, ASAL AIR PUTIH DAN JENIS MINUMAN TERHADAP KRISTAL URIN PADA MAHASISWA AKKES 17 AGUSTUS 1945 SEMARANG Ikawati, Kartika; Maulida, Bunga Rahma
Jurnal Dunia Kesmas Vol 14, No 3 (2025): Volume 14 Nomor 3
Publisher : Persatuan Dosen Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kebiasaan konsumsi minuman pada mahasiswa mempunyai dampak yang signifikan terhadap sedimen urin. Volume air, asal/sumber air putih dan jenis minuman berpengaruh pada munculnya kristal dalam urin. Jenis minuman teh dan kopi memicu ekskresi urin sedangkan soda banyak mengandung fruktosa pencetus terbentuknya kristal kalsium oksalat. Penelitian ini bertujuan untuk mengetahui hubungan volume, asal air putih dan jenis minuman terhadap kristal urin pada mahasiswa AKKES 17 Agustus 1945 Semarang. Jenis penelitian ini adalah observasi analitik dengan rancangan crossectional. Populasi penelitian mahasiswa AKKES 17 Agustus 1945 Semarang semester V.  Jumlah sampel penelitian sebanyak 40 orang yang memenuhi kriteria inklusi dan diambil dengan teknik sampling purposive. Variabel independen penelitian adalah volume air yang diminum setiap hari, asal/sumber air putih dan jenis minuman lainnya. Pemeriksaan kristal urin dilakukan dengan pengamatan sedimen urin di bawah mikroskup. Data yang diperoleh dilakukan uji korelasi Kendalls tau. Hasil uji korelasi volume air minum, asal air putih dan jenis minuman terhadap kejadian kristal dalam urin didapatkan R ; 0.81, 0,76 dan 0,79 dan nilai signifikani /sig  untuk ketiga variabel 0.000 atau < 0,05. Dapat disimpulkan bahwa terdapat hubungan yang kuat dan signifikan antara volume air minum, asal air putih dan  jenis minuman terhadap kristal sedimen urin pada mahasiswa AKKES 17 Agustus 1945 Semarang Kata kunci ; volume, asal air putih, jenis minuman, krital urin
Pengaruh Durasi Penggunan Gadget Terhadap Aktivitas Fisik Dan Pola Sosialisasi Anak SD Maranatha 01 Semarang (Peran Moderator Status Pekerjaan Ibu) Ikawati, Kartika; Yuniarti, Christina Ary
Jurnal Ilmu Kedokteran dan Kesehatan Vol 12, No 11 (2025): Volume 12 Nomor 11
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v12i11.22671

Abstract

Teknologi gadget saat ini berkembang pesat dengan salah satu pengguna dominan anak SD usia 6-12 tahun. Durasi penggunaan yang berlebihan berpotensi mempengaruhi aktivitas fisik dan pola sosialisasi anak. Status pekerjaan ibu diduga menjadi salah satu faktor yang memoderasi hubungan tersebut. Tujuan penelitian ini untuk mengetahui pengaruh durasi penggunaan perangkat gadget terhadap aktivitas fisik dan pola sosialisasi anak SD Maranatha 01 Semarang dengan peran status pekerjaan ibu sebagai variabel moderator. Jenis penelitian ini eksplanatori dengan pendekatan crossectional. Populasi penelitian adalah siswa SD Maranatha 01 Semarang. Jumlah sampel penelitian 42 anak yang dipilih dengan teknik proporsionate random sampling. Kriteria inklusi penelitian yaitu anak SD yang berumur 6-12 tahun sehat fisik dan mental. Data diperoleh  melalui pengisian lembar kuesioner dan observasi langsung. Analisis data dengan uji regresi ordinal. Hasil penelitian menunjukkan rata-rata durasi penggunaan gadget anak adalah 1,7 jam. Tingkat aktivitas fisik dan pola sosialisasi anak pada kategori sedang (40,5%). Hasil analisis menunjukkan durasi penggunaan gadget terbukti secara signifikan berpengaruh terhadap aktivitas fisik (p=0.011) namun tidak berpengaruh terhadap pola sosialisasi anak (P=0.292). Status pekerjaan ibu memoderasi hubungan pengguanan gadget terhap aktivitas fisik anak (p=0.016), namun tidak terbukti memoderasi hubungan pengunaan gadget terhadap pola sosialisasi anak (p=0.995). Implikasi penelitian menunjukkan perlunya pengaturan waktu penggunaan gadged, penyediaan kegiatan fisik dan strategi pendampingan anak oleh ibu.
UJI IN VIVO TIKUS WISTAR DENGAN BIT MERAH (Beta vulgaris L ) UNTUK PERBAIKAN KELAINAN PROFIL DARAH AKIBAT PAPARAN PESTISIDA: - Kartika - Ikawati
Klinikal Sains : Jurnal Analis Kesehatan Vol. 11 No. 1 (2023): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i1.3334

Abstract

Pesticides increase the number of free radicals, triggering oxidative stress causing inflammation and blood profile abnormalities such as anemia, agranulocytosis, neutropenia and thrombopenia. One of the plants that is rich in antioxidants and nutrients to form blood cells is red beet (Beta vulgaris L). The purpose of this study was to determine the ability of red beet (Beta vulgaris L) to improve blood profile abnormalities due to pesticide exposure through in vivo test of Wistar male rats. This research is a laboratory experimental type with a Post Test Only Randomized Control Group Design. The sample of the study was 25 male Wistar rats which were randomly divided into 5 different treatment groups. Mice were exposed to 140 ml/day of pesticides and various doses of red beet ethanol extract for 14 days. The blood profile parameters examined were hemoglobin level, hematocrit, erythrocyte count and leukocyte count. Examination of blood profile parameters manually and automatically. Data analysis with ANOVA and Post Hoc tests. The results of the study proved that the ethanol extract of red beetroot was able to improve blood profile damage due to pesticide exposure in the form of a significant increase in hemoglobin levels, hematocrit values, erythrocyte counts and reduced leukocyte counts from leukocytosis (P<0.05). The optimal dose of red beet ethanol extract is 400 mg/kgBB. Red beets are able to improve blood profile abnormalities due to exposure to pesticides. Red beets are recommended as a non-pharmacological alternative to improve blood profile abnormalities due to exposure to pesticides.
MODIFIKASI AMONIUM OKSALAT DENGAN PEWARNA ALAMI BIT MERAH SEBAGAI FORMULA BARU REAGEN HITUNG JUMLAH TROMBOSIT Kartika - Ikawati; Agus Widodo
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5197

Abstract

Abstract Platelet count using the Brecker koncrete method with 1% Ammonium oxalate reagent has a weakness that it cannot color platelets so that they are difficult to count. Red beet contains natural dyes that can color platelet organelles. This study aims to prove the ability of Ammonium oxalate modification with natural red beet dye (Beta vulgaris L) as a new formula for platelet count reagents. Method. This type of research is a laboratory experiment with a comparison of platelet counts using 1% Ammonium oxalate reagent and modification of Ammonium oxalate-red beet extract in a ratio of 1: 1, 1: 2, 2: 1 and automatic methods. The object of research is EDTA venous blood from 30 samples of AAK 17 Semarang students taken using the convenience sampling technique. Manual platelet counts using the Brecker concrete method with a counting chamber and an automatic method using a hematology analyzer. The sharpness of platelet staining is assessed under a microscope. The comparison test uses Repeated Anova. The results of the study showed that the color of platelets examined with the Ammonium-bit modification reagent (1: 2) was sharper with an average number approaching the gold standard. The statistical test results obtained sig 0.069 (> 0.05) or no significant difference. Beet extract (Beta vulgaris L) has the potential as an alternative natural dye in manual platelet counts (Brecer koncrite) keywords : trombosit, red bit, Amonium oksalat
Efek Nefroprotektif Bit Merah (Beta vulgaris L) terhadap Gangguan Ginjal Akibat Paparan Campuran Pestisida : Study Eksperimen pada Tikus Wistar Kartika Ikawati; Armydha Dwi Susanti
INSOLOGI: Jurnal Sains dan Teknologi Vol. 4 No. 5 (2025): Oktober 2025
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/insologi.v4i5.6556

Abstract

One of the risk factors for kidney disorders is excessive pesticide exposure. Kidney dysfunction is characterized by an increase in urea and creatinine levels. Red beetroot (Beta vulgaris L) contains bioactive compounds that are considered capable of reducing the risk of kidney disorders caused by pesticide exposure. This study aimed to prove the nephroprotective effect of red beetroot (Beta vulgaris L) against kidney damage induced by mixed pesticide exposure through an experimental study on Wistar rats. This research used an experimental design with a post-test only randomized control group design. The study population consisted of male Wistar rats. A total of 36 rats were included as samples, with inclusion criteria of being healthy, aged 2–3 months, and weighing 150–200 g. Sampling was carried out randomly. The animal experiments were conducted at the Faculty of Mathematics and Natural Sciences, UNNES Semarang, while the examination of urea and creatinine levels was performed at the Laboratory of AKKES 17 August 1945 Semarang. Urea and creatinine levels were measured semi-automatically. Data were analyzed using ANOVA and Post Hoc tests. The results showed that pesticide exposure for 14 days significantly increased urea and creatinine levels (p = 0.000). Administration of red beetroot extract to rats exposed to pesticides significantly reduced urea and creatinine levels (p = 0.000), with the optimal dose being 400 mg/kg body weight. It can be concluded that red beetroot extract has a nephroprotective effect against kidney damage caused by mixed pesticide exposure.