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All Journal JBIO: jurnal biosains (the journal of biosciences) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pembelajaran dan Biologi Nukleus JURNAL KAJIAN VETERINER Indigenous Biologi Bioscientist : Jurnal Ilmiah Biologi
Sabuna, Alan Ch.
Universitas Kristen Artha Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary

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UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI PEWARNA ALAMI TENUN IKAT DARI DESA HUNDIHOPO , KECAMATAN ROTE TIMUR, KABUPATEN ROTE NDAO Sabuna, Alan Charis; Hendrik, Arnold Christian; Bullu, Novi I.
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 4 No 3 (2021): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v4i3.271

Abstract

Penelitian ini dilakukan untuk mengetahui potensi antioksidan dan antibakteri dari ekstrak pewarna alami tenun ikat dari desa Hundihopo, Rote Timur, Kabupaten Rote Ndao. Penelitian ini dilaksanakan pada bulan Oktober – November 2018 di Laboratorium Biosains Universitas Nusa Cendana Kupang untuk uji antioksidan, dan Laboratorium Biologi Universitas Kristen Artha Wacana untuk uji antibakteri. Penelitian ini menggunakan metode eksperimental di laboratorium yang terbagi menjadi dua tahap. Tahap pertama ekstraksi dengan memanaskan bahan pewarna dengan air, dilanjutkan tahap kedua uji antioksidan menggunakan metode DPPH dan uji antibakteri menggunakan metode difusi agar. Kesimpulan dari penelitian ini bahwa ekstrak pewarna alami mengkudu, mengkudu + loba, dan tarum memiliki nilai IC50 6367,5 ppm, 3707,77 ppm, dan 3037,5 ppm sehingga berpotensi sebagai sumber antioksidan alami kategori sangat lemah. Diameter zona hambat ekstrak pewarna alami mengkudu + loba memiliki diameter zona hambat 10,1 ± 0,22 mm pada konsentrasi 5000 ppm sehingga termasuk antibakteri alami kategori lemah.
Uji Aktivitas Antioksidan Ekstrak Biji Kepuh (Sterculia foetida) serta Potensinya dalam Uji Neutrofil untuk Imunitas Mencit (Mus musculus) Ngginak, James; Sabuna, Alan Ch.; Bullu, Novi Ivone; Koy, Vedy Yeriel
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15671

Abstract

The purpose of this study is to test the  antioxidant activity of kepuh seed extract (Sterculia foetida) as well as its potential in neutrophil testing for mouse immunity (Mus musculus). This study was experimental by looking at the number or ratio of neutrophils in mice that had been given stress treatment in the form of wounds on the body of mice after being given kepuh seed extract. The antioxidant activity test is carried out using the DPPH method, while the neutrophil test can be carried out using the flow cytometry method. The results of the antioxidant activity test using the DPPH method (IC 50) showed a result of 18.0361 mg/mL. The regression results of levels (mg/mL) vs antioxidant activity (%) showed that the higher the level of kepuh seed extract, the higher the percentage of antioxidant ability available. Based on the results of the research, it can be concluded that; (1) The concentration of 18.0361 mg/mL in chickpeas (Sterculia foetida) can inhibit free radicals in the body, so it can be said that chickpeas (Sterculia foetida) have strong antioxidant activity; (2) The higher the content of kepuh seed extract, the higher the percentage of antioxidant ability.
Co-Authors Bullu, Novi I. Bullu, Novi Ivone Daud, Yanti Delfi Yulianti Ledoh Egertha Karpada Arsun Banamtuan Mellissa E. S. Ledo Febriana Bani Utang Ferdinan Migu Yunus Fransina Th. Nomleni Hendrik, Arnold Christian James Ngginak Koy, Vedy Yeriel Mami Seko Manifani, Derven Mellissa E. S. Ledo, Egertha Karpada Arsun Banamtuan Merpiseldin Nitsae Rafael, Andriani Rupidara, Anggreini D.N Sonya Titin Nge Sriyanti Aoetpah Vianros Ana Ate