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UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI PEWARNA ALAMI TENUN IKAT DARI DESA HUNDIHOPO , KECAMATAN ROTE TIMUR, KABUPATEN ROTE NDAO Sabuna, Alan Charis; Hendrik, Arnold Christian; Bullu, Novi I.
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 4 No 3 (2021): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v4i3.271

Abstract

Penelitian ini dilakukan untuk mengetahui potensi antioksidan dan antibakteri dari ekstrak pewarna alami tenun ikat dari desa Hundihopo, Rote Timur, Kabupaten Rote Ndao. Penelitian ini dilaksanakan pada bulan Oktober – November 2018 di Laboratorium Biosains Universitas Nusa Cendana Kupang untuk uji antioksidan, dan Laboratorium Biologi Universitas Kristen Artha Wacana untuk uji antibakteri. Penelitian ini menggunakan metode eksperimental di laboratorium yang terbagi menjadi dua tahap. Tahap pertama ekstraksi dengan memanaskan bahan pewarna dengan air, dilanjutkan tahap kedua uji antioksidan menggunakan metode DPPH dan uji antibakteri menggunakan metode difusi agar. Kesimpulan dari penelitian ini bahwa ekstrak pewarna alami mengkudu, mengkudu + loba, dan tarum memiliki nilai IC50 6367,5 ppm, 3707,77 ppm, dan 3037,5 ppm sehingga berpotensi sebagai sumber antioksidan alami kategori sangat lemah. Diameter zona hambat ekstrak pewarna alami mengkudu + loba memiliki diameter zona hambat 10,1 ± 0,22 mm pada konsentrasi 5000 ppm sehingga termasuk antibakteri alami kategori lemah.
Uji Aktivitas Antioksidan Ekstrak Biji Kepuh (Sterculia foetida) serta Potensinya dalam Uji Neutrofil untuk Imunitas Mencit (Mus musculus) Ngginak, James; Sabuna, Alan Ch.; Bullu, Novi Ivone; Koy, Vedy Yeriel
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15671

Abstract

The purpose of this study is to test the  antioxidant activity of kepuh seed extract (Sterculia foetida) as well as its potential in neutrophil testing for mouse immunity (Mus musculus). This study was experimental by looking at the number or ratio of neutrophils in mice that had been given stress treatment in the form of wounds on the body of mice after being given kepuh seed extract. The antioxidant activity test is carried out using the DPPH method, while the neutrophil test can be carried out using the flow cytometry method. The results of the antioxidant activity test using the DPPH method (IC 50) showed a result of 18.0361 mg/mL. The regression results of levels (mg/mL) vs antioxidant activity (%) showed that the higher the level of kepuh seed extract, the higher the percentage of antioxidant ability available. Based on the results of the research, it can be concluded that; (1) The concentration of 18.0361 mg/mL in chickpeas (Sterculia foetida) can inhibit free radicals in the body, so it can be said that chickpeas (Sterculia foetida) have strong antioxidant activity; (2) The higher the content of kepuh seed extract, the higher the percentage of antioxidant ability.
The Development of Reference Book from Medicinal Plant Exploration Results in Rote Tengah Sub-district, Rote Ndao District Nge, SonyaSonya Titin M.; Ballo, Apriliana; Sabuna, Alan Ch.; Rupidara, Anggreini D.N.; Hendrik, Arnold Ch.
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7083

Abstract

The utilization of medicinal plants has become one alternative for communities to treat illnesses. This is because the use of medicinal plants, besides being relatively affordable, also does not cause side effects compared to the use of modern medicine or chemical-based drugs. This study aims to develop a reference book of medicinal plants in Rote Tengah Sub-district, Rote Ndao District. The development model used in this research is the ADDIE model (analysis, design, development, implementation, evaluation). The research results obtained from material expert testing showed a validity of 94.6%, design expert testing showed 95.5%, and media expert testing showed 100%. Overall, the percentage of assessments from all three experts indicates a value of 96.7% with a qualification of very valid (highly appropriate). This indicates a positive response to the developed medicinal plant reference book, which is deemed suitable for use as a learning resource. This is because the developed medicinal plant reference book has its own advantages, where the illustrations in the reference book are made with attractive designs, thus attracting students' attention and making it easier to understand