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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner MANDALIKA VETERINARY JOURNAL Alamtana
Agustin, Alfiana Laili Dwi
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : MANDALIKA VETERINARY JOURNAL

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Antibiotic Resistance in Salmonella sp. Isolated from Layer Chicken in North Lombok, West Nusa Tenggara Agustin, Alfiana Laili Dwi
Mandalika Veterinary Journal Vol. 5 No. 2 (2025): OKTOBER
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/mvj.v5i2.18082

Abstract

Resistance antibiotic Salmobnella sp. difficult to recover when treated. Antibiotic resistance can occur due to inappropriate use of antibiotics, such as incorrect dosage, incorrect indications, and incorrect use. This research was conducted based on reports from farmers that their chickens did not recover after being given antibiotics when they had diarrhea. The results of interviews with farmers show that they usually use antibiotics without advice from a veterinarian, they can buy antibiotics freely at poultry shops. The samples in this study were taken from egg-laying chicken farms in Santong village, North Lombok, West Nusa Tenggara. Samples were taken from farms with the most reported cases of persistent diarrhea, with 32 samples taken from two farms 16 samples each. Samples were taken using sterile cotton buds from chickens showing symptoms of chalky diarrhea, the results of the rectal swab from each chicken were inserted into the brain heart infusion (BHI) transport media and placed in an ice box to then be planted in the media in the laboratory. From BHI media, cotton buds were swabbed on XLD media, the colonies that grew were purified and then tested for gram and biochemistry. esult was determined by standard biochemical procedures using Bergeyʼs Manual. Identified salmonella bacteria were tested with the antibiotics trimethoprim, ampicillin, ciprofloxacin, penicillin G, and erythromycin. Isolation results from 32 samples tested, 22 samples showed Salmonella sp. isolates. The results of the antibiotic resistance test showed that 22 isolates were resistant to penicillin G, ciprofloxacin was no longer able to kill 21 isolates resistant, and 11 isolates were resistant to the antibiotics erythromycin and ampicillin. The evidence of antibiotic resistance in Salmonella sp. bacteria from layer chickens in North Lombok can have an impact on human health, one health and can be spread through livestock food.
Identifikasi Bacillus sp. Yang Diisolasi Dari Madu Liar Ningtyas, Novarina Sulsia Ista'In; Andrianto, memo; Agustin, Alfiana Laili Dwi; Mutmainnah, Mutmainnah
Mandalika Veterinary Journal Vol. 4 No. 2 (2024): OKTOBER
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/mvj.v4i2.13274

Abstract

Madu merupakan cairan manis yang didapatkan dari bagian tumbuhan yang disimpan di saram lebah. Manfaat madu sebagai antioksidan, antimikroba, regulator gula darah dan membantu meningkatkan imun tubuh. Madu yang beredar dan banyak dimanfaatkan oleh manusia bersal dari madu liar dan madu budidaya. Madu liar didapatkan dari alam liar dengan kata lain tidak dibudidayakan. Sebagian besar madu liar didapatkan dan dikemas secara tradisional. Hal ini dapat memicu adanya kontaminasi salah satunya bakteri. Penelitian ini bertujuan untuk mengidentifikasi Bacillus sp. yang terdapat pada 10 sampel madu liar dengan metode biokimia. Bakteri yang telah ditumbuhkan pada media agar kemudian akan diuji Katalase, uji Glukosa, uji Manitol,Laktosa, Sukrosa, Maltosa, SIM, Urea, SC, TSIA, dan Arabinosa. Hasil pada penelitian ini mendapatkan 3 jenis Bacillus yaitu 8 sampel Bacillus cereus (80%), 1 sampel Bacillus licheniformis (10%), 1 sampel  Bacillus coagulans (10%).
Co-Authors Amzani, Mazrur Andrianto, memo April, Berlian Ronaldi Atma, Candra Dwi Dina Oktaviana Fachry Abda El Rahman Fitri, Dian Yusnia Maratun Janah, Maratun Mutmainnah Mutmainnah Ningtyas, Novarina Sulsia Ista'In Tirtasari, Kunti