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Diningrat, Diky Setya
Jurusan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Negeri Medan
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Potensi Antioksidan Alami pada Ekstrak Kulit Buah Jamblang (Syzigium cumini (L.) Skeels) Menggunakan Metode DPPH (The Potency of Natural Antioxidant in The Rind Extract of Jamblang (Syzigium cumini (L.) Skeels) using DPPH Method) Sari, Ayu Nirmala; Kusdianti, Kusdianti; Diningrat, Diky Setya
JURNAL BIOS LOGOS Vol 8, No 1 (2018): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.8.1.2018.20593

Abstract

AbstrakStress oksidatif pada tubuh dapat memicu berbagai penyakit. Stress oksidatif ini disebabkan karena radikal bebas berlebih. Tubuh memerlukan antioksidan untuk mengurangi pengaruh radikal bebas dan meredam dampak negatifnya. Penelitian ini bertujuan untuk mengevaluasi daya antioksidan ekstrak kulit buah jamblang (Syzigium cumini (L.) Skeels) dengan menggunakan metode DPPH. Berdasarkan hasil pengujian daya antioksidan ekstrak kulit buah jamblang diketahui bahwa ekstrak kulit buah jamblang memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC50 sebesar 169.3. Hasil penelitian ini menunjukkan bahwa kulit buah jamblang berpotensi untuk dikembangkan sebagai sumber antioksidan alami bagi manusia.Kata Kunci : antioksidan, DPPH, ekstraksi, jamblang, radikal bebas, stress oksidatif AbstractOxidative stress can induce many diseases. Oxidative stress is caused by excessive free radicals in the body. The body required antioxidant to decrease and hush the negative effects of free radicals. The aim of this study was to evaluate the antioxidant potency of extract rind of jamblang (Syzigium cumini (L.) Skeels) using DPPH method. The result showed that the jamblang rind extract had moderate antioxidant activity and the IC50 value was 169.3.  Based on this result, jamblang rind was potential to be natural antioxidant for human.Keyword: antioxidant, DPPH, extraction, jamblang, free radicals, oxidative stress
Analisis GC-MS Senyawa Bioaktif Pencegah Penyakit Degeneratif Dari Ekstrak Etanol Kulit Buah Jamblang (Syzygium Cumini) Ayu Nirmala Sari; Kusdianti Kusdianti; Diky Setya Diningrat
Elkawnie: Journal of Islamic Science and Technology Vol 4, No 2 (2018)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v4i2.4143

Abstract

Penelitian ini bertujuan untuk membuat inventarisasi database antioksidan alami dalam level molekuler dari tanaman jamblang (Syzygium cumini) dengan pendekatan Gas Chromatography Mass Spectrometer (GCMS) sehingga dapat dijadikan dasar pengembangan S. cumini sebagai antioksidan alami alternatif untuk mencegah kerusakan sel, kanker dan menurunkan kejadian penyakit degeneratif. Penelitian ini difokuskan pada S. cumini sebagai salah satu tanaman berkhasiat obat yang sudah mulai diteliti dalam khasiatnya sebagai tanaman yang mengandung senyawa bioaktif. Kehadiran teknologi GCMS ditengah belum adanya database tanaman jamblang merupakan peluang alternatif untuk memperoleh metabolom. Database metabolom pada jamblang penting dimiliki untuk menjaga plasma nutfah dari ekploitasi berlebihan yang menyebabkan kepunahan organisme dan budidaya tanaman tersebut. Dengan adanya penelitian inventarisasi level molekuler senyawa antioksidan yang dikandung tanaman jamblang dapat meningkatkan aktivitas penelitian lanjut pemanfaatan tanaman jamblang yang bisa ditingkatkan dalam skala industri nantinya. Untuk menjawab tujuan penelitian ini dilakukan tahapan penelitian sebagai berikut, pada tahap awal dilakukan isolasi metabolom dari kulit buah tanaman jamblang dengan cara ekstraksi. Tahap kedua adalah metabolome analysis menggunakan GCMS. Keluaran dari penelitian ini adalah diperolehnya data tentang hasil GCMS yang memuat senyawa bioaktif yang terkandung pada kulit buah tanaman jamblang yang akan menjadi landasan pengembangan pemanfaatan kulit buah jamblang sebagai antioksidan alternatif pencegah penyakit degenerasi. Pada penelitian selanjutnya perlu dilakukan analisis yang sama pada bagian tanaman jamblang lain untuk melengkapi database yang sudah ada.This research aims to create an antioxidant inventory databases of the Jamblang (Syzygium cumini) plant by Gas Chromatography-Mass Spectrometer (GCMS) approach.  This database can be used as the basis for the development of S. cumini as an alternative natural antioxidant to prevent cell damage, cancer and reduce disease incidence degenerative. This research is focused on S. cumini as one of the medicinal plants that have been studied in its usefulness as a plant containing the bioactive compound. The presence of GCMS technology amid the absence of Jamblang plant database is an alternative opportunity to obtain metabolome. The metabolome database in jamblang is important to keep the germplasm from excessive exploitation causing the extinction of the organism and the cultivation of the plant. Molecular level inventory of antioxidant compounds contained jamblang plants can increase research activities further utilization of jamblang plants that can be increased in the industrial scale later. To answer the purpose of this research carried out the following stages of research, in the early stages of metabolome isolation from the skin of jamblang fruit by way of extraction. The second stage is the metabolome analysis using GCMS. The output of this research is to obtain data about GCMS results that bioactive compounds contained in jamblang fruit skin that will become the foundation of the development of jamblang fruit skin as alternative antioxidant prevention of degeneration disease. In the next research needs to be done the same analysis on the jamblang plant to complete the existing database
Analisis Ekstrak Etanol Tangkai Daun Buasbuas (Premna pubescens) Menggunakan Gas Chromatography Mass Spectrophotometer (GCMS) Diky Setya Diningrat; Martina Restuati; Kusdianti Kusdianti; Ayu Nirmala Sari; Erly Marwani
Elkawnie: Journal of Islamic Science and Technology Vol 4, No 1 (2018)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v4i1.3075

Abstract

Buasbuas (Premna pubescens) is a plant that is traditionally known to have medicinal properties. This study aims to determine the content of bioactive compounds contained in the petiole by Gas Chromatography Mass Spectrophotometer (GCMS) method. Preparation of ethanol extract of petiole using maceration method with 96% ethanol solvent. The study used gas chromatographic tools and mass spectra which were evaluated using MASSLAB program. The data obtained from the GCMS machine is then analyzed using the NCBI database pubchem software (https://pubchem.ncbi.nlm.nih.gov/). The results of this study indicate that the content of bioactive compounds on the petioles of buasbuas more than 50 libraries contains about 150 species of compounds with a range of RT and% area respectively 4.684 to 28.155 and 0.16 to 15.56%. The content of bioactive compounds shown this data indicates that very large potential of buasbuas plants to be explored and exploitation as a nutritious plant. The results of this study can be used as the foundation in the development program of the potential utilization of bioassemblance of buasbuas plants. In further research it is necessary to analyze the other parts of the plant and make comparisons to complete the available databases.
Effect of Ethanol Extract of Jamblang Aceh (Syzygium cumini) in Diabetic Mice (Mus musculus) and Its Potential As Anti-Diabetic Agent Ayu  Nirmala Sari; Januardi Januardi; Diky Setya Diningrat
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 1 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.5496

Abstract

Abstract : This study was designed to evaluate the anti-diabetic activity of the ethanol extract of Syzygium cumini leaves in alloxan-induced diabetes mice. The anti-diabetic activity of EDS was investigated in mice (Mus musculus SW.) Alloxan-induced diabetes. The effect of ethanol extract of Syzygium cumini leaves on normal blood glucose levels and oral glucose tolerance tests were studied in normoglycemic mice while the anti-diabetic effect was evaluated in alloxan-induced hyperglycemic mice. Ethanol extract of Syzygium cumini leaves (200 and 400 mg/kg) is given orally for 21 days. Glibenclamide (5 mg/kg, oral for 21 days) is used as a reference standard. Giving ethanol extract of Syzygium leaves causes a significant decrease in blood glucose levels in normoglycemic and hyperglycemic mice and also increases glucose tolerance test. Ethanol extract of Syzygium leaves reduces glycosylated hemoglobin levels, lactate dehydrogenase, and creatinine kinase in alloxan-treated mice. Ethanol extract of Syzygium leaves also improves TBARS oxidative stress parameters, catalase and superoxide dismutase activity and glutathione levels. The ethanol extract of Syzygium cumini leaves shows anti-diabetic activity through increased insulin secretion and this effect can be attributed to the content of flavonoids and phenolic compounds present in the ethanol extract of Syzygium cumini leaves.Abstrak : Penelitian ini dirancang untuk mengevaluasi aktivitas anti-diabetes dari ekstrak etanol daun Syzygium cumini (L) Skeels (EDS) pada mencit diabetes yang diinduksi aloksan. Aktivitas anti-diabetes EDS diselidiki pada mencit (Mus musculus SW.) diabetes yang diinduksi aloksan. Pengaruh ekstrak etanol daun Syzygium cumini (EDS) pada kadar glukosa darah normal dan uji toleransi glukosa oral dipelajari pada mencit normoglikemik sedangkan efek antidiabetik dievaluasi pada mencit hiperglikemik yang diinduksi aloksan. EDS (200 dan 400 mg/kg) diberikan secara oral selama 21 hari. Glibenclamide (5mg/kg, oral selama 21 hari) digunakan sebagai standar referensi. Pemberian EDS menyebabkan penurunan signifikan dalam kadar glukosa darah pada mencit normoglikemik dan hiperglikemik dan juga meningkatkan uji toleransi glukosa. EDS mengurangi kadar hemoglobin glikosilasi, laktat dehidrogenase, dan kreatinin kinase pada mencit yang diberi aloksan. EDS juga memperbaiki parameter stres oksidatif TBARS, aktivitas katalase dan superoksida dismutase dan kadar glutathione. Ekstrak etanol daun Syzygium cumini (EDS) menunjukkan aktivitas antidiabetik melalui peningkatan sekresi insulin dan efek ini dapat dikaitkan dengan kandungan flavonoid dan senyawa fenolik yang ada dalam ekstrak daun.
Needs Analysis on the Development of Ethnomedicine Books as a Student Learning Resource in Ethnobotany Courses Ummi Zaimah; Ashar Hasairin; Diky Setya Diningrat
Jurnal Pendidikan Biologi Vol 11, No 1 (2022): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v11i1.33693

Abstract

Book development is very important to do support the learning process because it is integrated with the competencies that must be mastered and learned by students. The first step before developing the book is to conduct a needs analysis. This activity aims to determine the level of student need for the availability of books as learning resources used during ethnobotany lectures on ethnomedicine material. This research was conducted on 35 student of biology at the State University of Medan. This research is a qualitative descriptive study using a survey method. Data collection was carried out using a closed questionnaire. The results showed that the analysis of learning materials, as many as 62.9% of respondents said that ethnomedicine is one of the difficult materials in ethnobotany courses. The highest percentage is when identifying plants used as medicine (54.3%). Based on the needs analysis, 54.3% of respondents needed ethnomedicine books to add insight, so that 51.4% of respondents strongly agreed to develop ethnomedicine books as learning resources. It can be concluded that it is necessary to develop an ethnomedicine book as a learning resource that is prepared based on student needs and adapted to the sub-CPMK of ethnobotany subjects on ethnomedicine material. This research is useful to determine the need for learning resources needed to overcome the problems faced by students in learning activities so that the products developed are in accordance with the needs of students.
The Development of Illustrated Pocket Book as a Source of Independent Learning in Sensory System Material in XIth Grade of High School Natural Science Program Muliawati Muliawati; Binari Manurung; Diky Setya Diningrat
Jurnal Pendidikan Biologi Vol 10, No 1 (2021): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v10i1.22775

Abstract

Learning resources are a component needed in teaching and learning activities. One example of a learning resources often used by teachers and students is books. This study aims to develop a pocket book with a sensory system as a source of independent learning and to determine the effectiveness of a sensory system illustrated pocket book on student learning outcomes in the XIth Grade High School Natural Science Program. This type of research is Research and Development using the 4D Thiagarajan model (Define, Design, Development and Dissemination). The effectiveness test used a quasi experimental method with the pretest post-test control group design. The data obtained were both quantitative and qualitative. The results of the validation by material experts scored 95.9 (very feasible), language experts scored 90.77 (very feasible), and layout design experts scored 83.48 (very feasible). The results of the response by the Biology teacher were 99.58 (very feasible), and the results of student responses on individual tests were 97.78, small group tests were 98, and limited field tests were 99.72 under very feasible category. The effectiveness of sensory system illustrated pocket book is seen based on the students' Biology learning outcomes, t-test and N-gain value. The results showed that the sensory system illustrated pocket book as an independent learning resource was very feasible to use in biology learning and was quite effective in improving student learning outcomes in the XIth Grade of High School Natural Science Program.
ISOLASI SENYAWA BIOINSEKTISIDA PADA EKSTRAK ETANOL TUMBUHAN BUASBUAS (Premna pubescens Blume) DENGAN METODE GCMS Diky Setya Diningrat; Sonya Adelina Sipayung; Martina Restuati; Erly Marwani; Ayu Nirmala Sari; Kusdianti Kusdianti
Prosiding Seminar Nasional Biotik Vol 6, No 1 (2018): PROSIDING SEMINAR NASIONAL BIOTIK VI 2018
Publisher : Prosiding Seminar Nasional Biotik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.492 KB) | DOI: 10.3126/pbio.v6i1.4312

Abstract

Penelitian ini bertujuan untuk mengetahui pada bagian tumbuhan buasbuas (Premna pubescens Blume) manakah senyawa insektisida terakumulasi dan membandingkan jenis senyawa insektisida yang terdapat di daun muda, daun tua, tangkai daun, dan buah dengan teknik Gas Chromatography – Mass Spectrometry (GC-MS). Ekstrak buasbuas dibuat menggunakan metode maserasi dengan pelarut etanol 96% dan di angin-anginkan hingga menjadi pasta. Pasta yang didapatkan kemudian dianalisis dengan GC-MS. Data yang diperoleh dari hasil GC-MS yang diidentifikasi dengan software PubChem (https://pubchem.ncbi.nlm.nih.gov). Hasil penelitian menunjukkan adanya senyawa 7-methyl-1-benzofuran yang merupakan insektisida terakumulasi pada daun muda sebanyak 0,011% dari total 87 senyawa bioaktif. Pada bagian tumbuhan buasbuas lainya tidak ditemukan senyawa insektisida. Pada daun muda dan daun tua ditemukan herbisida yaitu kelompok senyawa yang sama dengan insektisida tergolong kedalam senyawa “cydal”. Kesimpulan penelitian ini adalah senyawa insektisida terdapat pada tanaman buasbuas, meskipun hanya satu jenis senyawa insektisida yang ditemukan dari keseluruhan senyawa bioaktif tanaman buasbuas yang diidentifikasi.
PENGARUH PEMBERIAN EKSTRAK DAGING BUAH ASAM KERANJI (Dialium indum L.) TERHADAP KADAR KOLESTEROL MENCIT JANTAN (Mus musculus) HIPERLIPIDEMIA Ayu Nirmala Sari; Muslich Hidayat; Siti Faizah; Diky Setya Diningrat
Biofaal Journal Vol 2 No 1 (2021): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.649 KB) | DOI: 10.30598/biofaal.v2i1pp1-10

Abstract

Masyarakat Aceh cenderung memiliki rutinitas pagi yang sama setiap harinya, yaitu sarapan dan minum kopi di waktu pagi. Hal ini dibuktikan dengan hasil observasi yang dilakukan dalam rentang waktu 30 hari pada beberapa warung kopi di Daerah Darussalam dari tanggal 3 Oktober hingga 1 November 2018. Pilihan makanan masyarakat Aceh juga cenderung sama.Hal ini didukung oleh hasil kuesioner yang diberikan kepada 75 responden warga Banda Aceh pada tanggal 8 November 2018, di sekitar Darussalam. Sebesar 60 % responden mengaku senang mengkonsumsi sambal balado, 20% gorengan, dan 20% kuah berlemak setiap hari. Padahal, makanan tersebut merupakan sumber kolesterol yang apabila dikonsumsi dalam keadaan tinggi dan terus menerus dapat menimbulkan resiko penyakit jantung. Obat alami dari tanaman dapat dijadikan solusi alternatif untuk penanganan kolesterol tinggi. Satu diantara jenis tanaman lokal Aceh yang memiliki khasiat sebagai antikolesterol adalah asam keranji (Dialium indum L.). Hasil penelitian membuktikan bahwa pemberian ekstrak daging buah asam keranjiberpengaruh terhadapkadarkolesterol darah mencit jantan (Mus musculus). Dosis 100 mg/kgBB merupakan dosis optimum dalam menurunkan kadar kolesterol darah mencit (Mus musculus) hiperlipidemia.
POTENSI EKSTRAK DAUN ASAM KERANJI (Dialium indum) ACEH SEBAGAI ANTIOKSIDAN ALAMI Syafrina Sari Lubis; Ayu Nirmala Sari; M. Haikal Fahmi; Diky Setya Diningrat
Biofaal Journal Vol 2 No 1 (2021): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.364 KB) | DOI: 10.30598/biofaal.v2i1pp11-18

Abstract

Penelitian ini dilakukan untuk menguji aktivitas fitokimia ekstrak etanol asam keranji (Dialium indum) sebagai antioksidan secara in vitro. Aktivitas antioksidan yang dilakukan dalam beberapa model pengujian antioksidan secara in vitro yaitu menguji aktivitas reduksi radikal bebas DPPH, radikal superoksida, daya pereduksi ferric dan aktivitas hidrogen peroksida. Kapasitas total antioksidan juga ditentukan. Ekstrak etanol asam keranji menunjukkan aktivitas antioksidan dengan menghambat DPPH, superoksida dan hidrogen peroksida. Pada penelitian ini juga menunjukkan kemampuan ekstrak etanolasam keranji mengurangi kemampuan dalam mereduksi besi. Kapasitas total antioksidan ekstrak etanol asam keranji17,66 mg/g yang dibandingkan dengan asam askorbat (Vitamin C). Aktivitas antioksidan yang signifikan dari ekstrak etanol Dialium indum diduga disebabkan oleh adanya senyawa Asam, Flavonoid, Fenol, Saponin, Tannin (senyawa Fenolik) dan Triterpenoid yang ditemukan dalam screening fitokimia sebelumnya.
Effect of Jungga Orange Peel Essential Oil (Citrus Jambhiri) on Platelet Function in Male Rats Given High Intensity Physical Activity Novita Sari Harahap; Diky Setya Diningrat; Yetty Machrina
Kinestetik : Jurnal Ilmiah Pendidikan Jasmani Vol 6 No 3 (2022): SEPTEMBER (ACCREDITED SINTA 3)
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jk.v6i3.23764

Abstract

Physical activity is one of the stress factors that can provide physiological changes to the function of the hematological system such as changes in platelet count. Physical activity can produce free radicals that can result in cell damage including platelets. Platelets play a role in the hemostasis process, If the platelet count is too low, it can cause excessive bleeding. However, if there is an increase in platelet count too high, it can cause thrombosis that can inhibit blood vessels. Antioxidants have a protective effect on cells and eliminate free radicals. The objective of this research was to ascertain the reaction of platelets to the injection of jungga orange peel essential oil (citrus jambhiri) in male rats that were subjected to strenuous exercise. Twenty male rats (3-4 months old, 180-200 grams) of the Wistar strain of the white rat (Rattus norvegicus) were used in the experiment. Using a random number generator, 20 white rats were split into two groups. In group P1, the rats swam for 50 minutes, three times a week, for four weeks. In group P2, the rats swam for 50 minutes, three times a week, for four weeks, and were also given 0.05 milliliters of jungga orange peel essential oil every hour prior to swimming. Based on these findings, it appears that there is a statistically significant difference between the P1 and P2 groups in terms of the average platelet count, with the P2 group exhibiting a decline in platelet count. Antioxidant substances contained in the essential oil of jungga orang have the ability to inhibit the decline in platelet function after high-intensity physical activity.
Co-Authors Abdul Muin Sibuea Acong Jaya Sinaga Adi Hartono Angga Karisto Bangun Ashar Hasairin Asmin Asmin, Asmin Ayu Nirmala Sari Ayu Nirmala Sari Binari Manurung Binari Manurung Budiarta, Kustoro Erly Marwani, Erly Fahri Setya Maulana Faisal Faisal Farhana Setya Farhana Fatma Tresno Ingtyas Febi Anjaini Purba Ginting, Alin Anggreni Gustianti Dewi Harahap, Novita Sari Hodriani Hodriani Januardi Januardi Janwardi Reywanda Tarigan Kusdianti - Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti Kusdianti M. Haikal Fahmi Maulana, Bagoes Muliawati Muliawati Muslich Hidayat Novita Sari Harahap Novita Sari Harahap Novita Sari Harahap Nurmala Berutu Ramadanu Ramadanu Restuati, Martina Risna Lenita Nasution Siregar, Batumahadi Siska Aidarahmi Siti Faizah Sonia Fitriani Sonya Adelina Sipayung Syafrina Sari Lubis Ummi Zaimah Winarsih - Yetty Machrina Zulaini Zulaini