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All Journal E-Journal Widya Kesehatan dan Lingkungan Pro-Life Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)
Rahmawati, Fri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

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Potensi Antioksidan, Skrining, dan Identifikasi Metabolit Sekunder Ekstrak Beras Hitam (Oryza sativa L. Indica) Rahmawati, Fri; Bintang, Maria; Yang, Albert Jackson; Damayanti, Ni Made Devi
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Black rice (Oryza sativa L. indica) is a variety of pigmented rice that, apart from acting as a popular food ingredient, can also be used for health because it has active compounds that have the potential to act as antioxidants. The research carried out aimed to determine antioxidant potency, conduct secondary metabolite screening, and characterize the active compound content of black rice extract. It was macerated using a 96% pro-analysis (p.a.) ethanol solvent. Antioxidant potency was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods, secondary metabolite screening using the Harbone method, and identification of active compounds using Gas Chromatography-Mass Spectrometry (GC-MS). It was found that black rice extract has potential as an antioxidant, using the DPPH method, the 50% Inhibition Concentration (IC50) value of black rice extract and vitamin C was 97.34 ppm and 2.64 ppm, respectively, but using the FRAP method, the IC50 values of black rice extract and vitamin C were 181.83 ppm and 7.84 ppm, respectively. Secondary metabolite screening detected that in black rice extract there were active compounds in the flavonoid and steroid groups, GC-MS identification results showed that the extract contained the most compound, hexadecanoic acid.
ANTIOKSIDAN RUMPUT KEBAR (Biophytum umbraculum) Rahmawati, Fri; Bintang, Maria; Yang, Albert Jackson; Talakua, Hazella Ishera
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

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Abstract

Oxidative stress is one of the causes of heart disease, atherosclerosis, and cancer. Efforts to overcome oxidative stress include the use of natural antioxidants. Flavonoids and phenolics are secondary metabolite compounds in plants that have antioxidant activity. Plants that are believed to be able to overcome oxidative stress include Kebar grass. Kebar grass (Biophytum umbraculum) is one of the plants that empirically has many benefits including as a female fertility drug by the Kebar-Papua community. The study aimed to determine the antioxidant potential and total phenolic and flavonoid content of Kebar grass extract. Kebar grass extract was obtained through maceration extraction with 96% pro-analysis (p.a) ethanol solvent. Antioxidant testing used the 2.2-diphenyll-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods using antioxidant standards, namely ascorbic acid (vitamin C) and gallic acid, while measuring total phenolic and flavonoid levels used the Folin-Ciocalteu and aluminium chloride methods, respectively. The results showed that the total phenolic and flavonoid levels of Kebar grass extract were 52.26 ± 3.94 mg GAE/g dw (dry weight) and 22.15 ± 2.79 mg QE/g dw Kebar grass extract, respectively, while the antioxidants of Kebar grass extract with the DPPH method had an inhibition concentration 50 (IC50) value of 183.44 ppm and a vitamin C standard of 4.46 ppm, and antioxidant testing using the FRAP method obtained a reducing power 50 (RP50) value of Kebar grass extract of 5043.76 ppm with a gallic acid standard value of 101.8 ppm.
Co-Authors Albert Jackson Yang Damayanti, Ni Made Devi Langi, Louisa Ariantje MARIA BINTANG Pariama, Ganda Reviani, Nia Talakua, Hazella Ishera Ule Lagno, Selviana Santisima Wija, Ida Bagus Eka Utama