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All Journal E-Journal Widya Kesehatan dan Lingkungan Pro-Life Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)
Rahmawati, Fri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

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SKRINING GOLONGAN SENYAWA AKTIF DAN ANALISIS TOKSISITAS EKTRAKS BIJI KABAU (Archidendron Bubalinum) Rahmawati, Fri
E-Journal Widya Kesehatan dan Lingkungan Vol. 1 No. 2 (2018)
Publisher : E-Journal Widya Kesehatan dan Lingkungan

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Abstract

Kabau (Archidendron bubalinum) merupakan salah satu tanaman yang sering digunakan sebagai bahan makananan, namun juga memiliki potensi untuk dikembangkan dalam bidang kesehatan. Bagian kabau yang sering dimanfaatkan sebagai bahan makanan adalah biji. Tujuan penelitian ini dilakukan untuk mengetahui kandungan golongan senyawa aktif (fitokimia) dan tingkat toksisitas biji kabau. Skrining golongan senyawa aktif biji kabau dilakukan menggunakan metode Harbone dan analisis toksisitas biji kabau dengan metode Brine Shrimp Lethality Test (BSLT). Bagian biji kabau yang digunakan dalam penelitian adalah daging dan kulit ari dari biji kabau. Biji kabau dimaserasi menggunakan pelarut 70% dan 99.9% etanol pro analisis. Hasil penelitian menunjukkan bahwa ekstrak kulit ari biji kabau mengandung flavonoid, tanin dan saponin sedangkan ekstrak kulit ari etanol 99.9% mengandung steroid. Ekstrak kulit ari biji kabau etanol 70% memiliki tingkat toksisitas terkecil dengan nilai LC50 sebesar 896.08 ppm.
Potensi Antioksidan, Skrining, dan Identifikasi Metabolit Sekunder Ekstrak Beras Hitam (Oryza sativa L. Indica) Rahmawati, Fri; Bintang, Maria; Yang, Albert Jackson; Damayanti, Ni Made Devi
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Black rice (Oryza sativa L. indica) is a variety of pigmented rice that, apart from acting as a popular food ingredient, can also be used for health because it has active compounds that have the potential to act as antioxidants. The research carried out aimed to determine antioxidant potency, conduct secondary metabolite screening, and characterize the active compound content of black rice extract. It was macerated using a 96% pro-analysis (p.a.) ethanol solvent. Antioxidant potency was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods, secondary metabolite screening using the Harbone method, and identification of active compounds using Gas Chromatography-Mass Spectrometry (GC-MS). It was found that black rice extract has potential as an antioxidant, using the DPPH method, the 50% Inhibition Concentration (IC50) value of black rice extract and vitamin C was 97.34 ppm and 2.64 ppm, respectively, but using the FRAP method, the IC50 values of black rice extract and vitamin C were 181.83 ppm and 7.84 ppm, respectively. Secondary metabolite screening detected that in black rice extract there were active compounds in the flavonoid and steroid groups, GC-MS identification results showed that the extract contained the most compound, hexadecanoic acid.
BIOAKTIVITAS KROKOT (Portulaca grandiflora Hook) SECARA IN VITRO Rahmawati, Fri; Bintang, Maria; Yang, Albert Jackson; Ule Lagno, Selviana Santisima
Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Vol 6 No 01 (2024): HERCLIPS VOL 06 NO 01
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/herclips.v6i01.7977

Abstract

Krokot (Portulaca grandiflora Hook) adalah gulma yang berkhasiat obat dan secara empiris digunakan masyarakat untuk berbagai pengobatan seperti mengatasi diare dan penyembuhan luka. Penelitian bertujuan untuk mengetahui potensi antibakteri, antioksidan dan toksisitas ekstrak krokot. Jenis penelitian berupa eksperimental laboratorium menggunakan sampel campuran batang dan daun krokot. Ekstrak krokot diperoleh dari hasil maserasi dengan etanol 96 % pro analisis (p.a). Pengujian antibakteri menggunakan metode difusi, antioksidan memakai metode 1,1-difenil-2-pikril-hidrazi (DPPH) serta toksisitas dengan metode Brine Shrimp Lethality Test (BSLT). Escherichia coli (E. coli) dan Staphylococcus aureus (S. aureus) digunakan sebagai bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak krokot dapat menghambat pertumbuhan bakteri pada konsentrasi hambat minimum (KHM) sebesar 40% pada E. coli dan 60% terhadap S. aureus dengan menghasilkan zona hambat masing- masing sebesar 6,34 mm dan 6,40 mm. Ekstrak krokot berpotensi sebagai antioksidan kuat dengan nilai Inhibition Concentration 50 % (IC50) sebesar 11,890 ppm, sedangkan vitamin C sebagai kontrol positif sebesar 2,658 ppm. Ekstrak krokot bersifat toksik pada larva udang dengan nilai Lethal Concentration 50% (LC50) sebesar 168,904 ppm. Sehingga disimpulkan krokot mempunyai bioaktivitas antioksidan, antibakteri dan toksisitas.
ANTIOKSIDAN RUMPUT KEBAR (Biophytum umbraculum) Rahmawati, Fri; Bintang, Maria; Yang, Albert Jackson; Talakua, Hazella Ishera
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

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Abstract

Oxidative stress is one of the causes of heart disease, atherosclerosis, and cancer. Efforts to overcome oxidative stress include the use of natural antioxidants. Flavonoids and phenolics are secondary metabolite compounds in plants that have antioxidant activity. Plants that are believed to be able to overcome oxidative stress include Kebar grass. Kebar grass (Biophytum umbraculum) is one of the plants that empirically has many benefits including as a female fertility drug by the Kebar-Papua community. The study aimed to determine the antioxidant potential and total phenolic and flavonoid content of Kebar grass extract. Kebar grass extract was obtained through maceration extraction with 96% pro-analysis (p.a) ethanol solvent. Antioxidant testing used the 2.2-diphenyll-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods using antioxidant standards, namely ascorbic acid (vitamin C) and gallic acid, while measuring total phenolic and flavonoid levels used the Folin-Ciocalteu and aluminium chloride methods, respectively. The results showed that the total phenolic and flavonoid levels of Kebar grass extract were 52.26 ± 3.94 mg GAE/g dw (dry weight) and 22.15 ± 2.79 mg QE/g dw Kebar grass extract, respectively, while the antioxidants of Kebar grass extract with the DPPH method had an inhibition concentration 50 (IC50) value of 183.44 ppm and a vitamin C standard of 4.46 ppm, and antioxidant testing using the FRAP method obtained a reducing power 50 (RP50) value of Kebar grass extract of 5043.76 ppm with a gallic acid standard value of 101.8 ppm.
Education on the Importance of Breast Milk and Complementary Foods for Breast Milk (MPASI) as an Effort to Prevent Stunting Langi, Louisa Ariantje; Rahmawati, Fri; Wija, Ida Bagus Eka Utama; Pariama, Ganda; Reviani, Nia
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 1 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i1.378

Abstract

Stunting is a form of growth failure (growth faltering) due to the accumulation of nutritional deficiencies that last for a long time starting from pregnancy until the age of 24 months. Optimal nutritional intake for newborns by providing exclusive breast milk as the main food until the age of 6 months and then continuing until the age of 2 years accompanied by appropriate and high-quality complementary foods will prevent stunting in toddlers. One important effort to prevent stunting is by empowering the community, especially parents, to pay attention to the intake of breast milk and complementary foods for their babies. By providing knowledge to the public about the importance of correct breastfeeding and MPASI, it is hoped that the public can change their behavior to prioritize exclusive breastfeeding and correct MPASI. This community service method uses an educational approach through outreach with a total of 139 participants who live in five villages in Jatigede District, Sumedang Regency, West Java. Socialization involving experts gave results that there was an increase in participants' knowledge as measured using pre-test and post-test instruments. The percentage of participants who experienced an increase in knowledge from 139 participants, the results obtained were 118 participants (85%) experienced an increase in knowledge about breast milk and complementary foods, and 19 people (14%) had the same knowledge before and after socialization while 2 people (1%) gained The score decreased during the post-test due to several factors, including some participants who did not have time to complete the post-test questionnaire due to having to do other work
Co-Authors Albert Jackson Yang Damayanti, Ni Made Devi Langi, Louisa Ariantje MARIA BINTANG Pariama, Ganda Reviani, Nia Talakua, Hazella Ishera Ule Lagno, Selviana Santisima Wija, Ida Bagus Eka Utama