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All Journal AL KAUNIYAH Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUSCIENCE Jurnal Pengabdian Magister Pendidikan IPA Makara Journal of Science
Yuniati, Ratna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Materials Science & Nanotechnology Physics

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PENGARUH KONSENTRASI MEDIUM EKSTRAK TAUGE (MET) TERHADAP PERTUMBUHAN Scenedesmus ISOLAT SUBANG Prihantini, Nining Betawati; Damayanti, Dini; Yuniati, Ratna
Makara Journal of Science Vol. 11, No. 1
Publisher : UI Scholars Hub

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Abstract

The effect of Tauge Extract Medium (TEM) concentration to the growth of Subang isolated Scenedesmus. Research on the effect of several concentration of Tauge Extract Medium (TEM) to Scenedesmus Meyen cell densities was conducted. The research was an experimental study with complete random design with 8 treatments, i.e. 1%, 2%, 3%, 4%, 5%, 6% TEM, aqua bides, and Bold Basal Medium (BBM). The results showed that TEM concentration effected cell densities of Scenedesmus. At peak condition, the highest cell density (3.981.071 sel/mL) was occurred in 4% at the day of seven, and the lowest cell density (87.096 sel/mL) in aqua bides at the day of three. Kruskal-Wallis test showed that there were differences between the mean of cell densities on all treatment media. Multiple comparison test showed that mean of cell numbers of Scenedesmus differed among treatments (p value>0,05) between aqua bides with 1%, 2%, 3%, 4%, 5%, 6%, and BBM.
PENAPISAN GALUR KEDELAI Glycine max (L.) Merrill TOLERAN TERHADAP NaCl UNTUK PENANAMAN DI LAHAN SALIN Yuniati, Ratna
Makara Journal of Science Vol. 8, No. 1
Publisher : UI Scholars Hub

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Screening of Soybean Cultivars Glycine max (L.) Merrill under Sodium Chloride Stress Condition. Salinity is one of the most serious and widespread agricultural problems resulting in losses of yield. Generally, as land is more intensively cultivated, the salinity problem becomes more severe. A high concentration of NaCl greatly reduces growth of both the shoot and the root. One strategy available to cope with saline soil is to choose salt-tolerance crops or to select salt-tolerance cultivars within a crop. Experiments were conducted to asses the performance of ten cultivars soybean (Glycine max [L.] Merrill) to salt stress at germination and seedling stages. Salinity treatments were begun by adding 70, 80, 90, and 100 mM NaCl to the basal nutrient solution. According to germination percentage, fresh weight/dry weight ratios, and the percentage of dead apical buds we suggest that Wilis, Malabar and Sindoro were tolerant lines, Genjah Jepang, Lokan, and Tidar were moderate and the sensitive lines were Lumut, Yellow Biloxy, Si Cinang and Sriyono.
ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; Suharsono, Suharsono
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.
Mangifera spp. in the Ethnoclimatology Study of Tatangar Banjar Tradition in Kalimantan Hidayati, Eisya Hanina; Yuniati, Ratna; Lestari, Retno; Jumari, Jumari; Walujo, Eko Baroto
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30048

Abstract

The biodiversity of Mangifera spp. not only supports the local economy and preserve food security but also safeguards cultural heritage through their role in ethnoclimatology studies within the Tatangar tradition of the Banjar community in Kalimantan. The Banjar community demonstrates a unique connection with nature by utilizing specific Mangifera species as crucial indicators of seasonal transitions, deeply ingrained in their cultural identity. The aim of this study is to reveal the prevalence of various Mangifera species, including endangered ones, in diverse landscapes, promoting a sustainable agroforestry system and conservation efforts. Qualitative and quantitative research methods were employed to explore and analyze Tatangar knowledge within the Banjar community in Mandiangin Barat Village, particularly its connections to climate and weather. The Banjar community further traditionally classifies Mangifera species, or known as “asam-asaman”, based on morphological traits, aiding in their interpretation of Tatangar signs. These signs, rooted in the flowering time of Mangifera trees, serve as essential climate indicators, guiding agricultural practices and influencing decisions on planting and harvesting. This local knowledge enhances agricultural resilience, enriches their cultural heritage, and underscores the intrinsic relationship between culture, nature, and sustainability in Kalimantan, making it a valuable subject for ethnoclimatological exploration
Mangifera spp. in the Ethnoclimatology Study of Tatangar Banjar Tradition in Kalimantan Hidayati, Eisya Hanina; Yuniati, Ratna; Lestari, Retno; Jumari, Jumari; Walujo, Eko Baroto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30048

Abstract

The biodiversity of Mangifera spp. not only supports the local economy and preserve food security but also safeguards cultural heritage through their role in ethnoclimatology studies within the Tatangar tradition of the Banjar community in Kalimantan. The Banjar community demonstrates a unique connection with nature by utilizing specific Mangifera species as crucial indicators of seasonal transitions, deeply ingrained in their cultural identity. The aim of this study is to reveal the prevalence of various Mangifera species, including endangered ones, in diverse landscapes, promoting a sustainable agroforestry system and conservation efforts. Qualitative and quantitative research methods were employed to explore and analyze Tatangar knowledge within the Banjar community in Mandiangin Barat Village, particularly its connections to climate and weather. The Banjar community further traditionally classifies Mangifera species, or known as “asam-asaman”, based on morphological traits, aiding in their interpretation of Tatangar signs. These signs, rooted in the flowering time of Mangifera trees, serve as essential climate indicators, guiding agricultural practices and influencing decisions on planting and harvesting. This local knowledge enhances agricultural resilience, enriches their cultural heritage, and underscores the intrinsic relationship between culture, nature, and sustainability in Kalimantan, making it a valuable subject for ethnoclimatological exploration
Effect of EM4 (Effective Microorganism 4) on Growth and Productivity of Cucumber (Cucumis sativus L.) Yuniati, Ratna; Damayanti, Margaretta Elsa; Wardhana, Wisnu
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37561

Abstract

AbstractCucumber (Cucumis sativus L.) is a nutritious and healthy vegetable that is commonly consumed by Indonesian people. To fulfill self-sufficiency for household scale needs during the COVID-19 pandemic, cucumber cultivation can be carried out in home gardens, using containers such as polybags. Growing cucumbers on limited land requires a carefully optimized planting media composition by applying Effective Microorganism 4 (EM4) to the polybag media when planting. The research has been conducted which aims to determine the best EM4 dosage for the growth and productivity of cucumbers. The study used a Randomized Block Design consisting of control and three treatment doses of 10% concentration EM4, namely 20, 40, and 60 mL per polybag with six replications. The planting media used is a mixture of loam soil and goat manure. NPK fertilizer is given as an additional nutrient. The EM4 application is done by pouring it every eight days into the planting media in polybags. The results showed an increase in growth parameters and productivity of cucumber plants namely plant height, leaf chlorophyll content, time of flower emergence, number of flowers, and number of flowers that form fruit. 40 mL EM4 is the dose that showed the highest growth and productivity.AbstrakMentimun (Cucumis sativus L.) merupakan sayuran bergizi dan menyehatkan yang banyak dikonsumsi masyarakat Indonesia. Untuk memenuhi swasembada kebutuhan skala rumah tangga di masa pandemi COVID-19, budidaya mentimun dapat dilakukan di pekarangan rumah, dengan menggunakan wadah polybag. Menanam mentimun di lahan terbatas memerlukan optimalisasi komposisi media tanam secara cermat dengan menerapkan Effective Microorganism 4 (EM4) pada media dalam polybag. Telah dilakukan penelitian yang bertujuan untuk mengetahui dosis EM4 terbaik untuk pertumbuhan dan produktivitas tanaman mentimun. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri atas kontrol dan tiga perlakuan dosis EM4 konsentrasi 10% yaitu 20, 40, dan 60 mL per polybag dengan enam ulangan. Media tanam yang digunakan adalah campuran tanah lempung dan kotoran kambing. Pupuk NPK diberikan sebagai unsur hara tambahan. Penerapan EM4 dilakukan dengan cara disiram setiap delapan hari sekali ke dalam media tanam di polybag. Hasil penelitian menunjukkan adanya peningkatan parameter pertumbuhan dan produktivitas tanaman mentimun yaitu tinggi tanaman, kandungan klorofil daun, waktu munculnya bunga, jumlah bunga, dan jumlah bunga yang membentuk buah. Dosis yang menunjukkan pertumbuhan dan produktivitas tertinggi adalah 40 mL EM4.
Comparative Study of The Physiological Condition of Six Tree Species to Air Pollution in Depok City, West Java Ayunda, Ajenk; Yuniati, Ratna; Handayani, Windri
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/9749

Abstract

Background: Air pollution is a threat to the environment. Sources of air pollutants in urban environments can be in the form of dust, heavy metals, and hydrocarbons. Plants can help clean air pollutants from the atmosphere by absorption through the stomata, accumulating them, or by adsorption on the leaf surface. The Air Pollution Tolerance Index (APTI) is used as an evaluation benchmark for the Physiological conditions of plants exposed to air pollution. This research aims to study the physiological conditions of six tree species in air conditions in the Depok City area and to assess the plants' tolerance level based on the APTI calculation. Methods: The physiological parameters measured to calculate APTI were ascorbic acid, total chlorophyll, leaf extract pH, and Relative Water Content (RWC). The six tree species used as objects in this study were Artocarpus altilis, Artocarpus heterophyllus, Bauhinia purpurea, Ficus septica, Filicium decipiens, and Nephelium lappaceum. Results: Differences in the physiological conditions of six tree species in the Depok City area based on the average ascorbic acid values, total chlorophyll, leaf extract pH, and RWC. In addition, there are also differences in APTI parameters between the two research locations. Conclusions: Filicium decipiens is the plant species with the highest APTI, while Artocarpus heterophyllus has the lowest. Based on the APTI scores, Bauhinia purpurea, Ficus septica, Filicium decipiens, Nephelium lappaceum belong to moderately tolerant category, Artocarpus altilis belongs to an intermediate category, Artocarpus heterophyllus belongs to sensitive category.
A Review of Fungal Disease in Hevea brasiliensis (Willd. ex A. Juss.) Mull. Arg.: From Identification to Scientific Investigation for Control Strategies Ainusyifa, Fathia; Lestari, Retno; Yuniati, Ratna
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9388

Abstract

Fungal diseases pose a substantial risk to the health and productivity of rubber plants (Hevea brasiliensis (Willd. ex ​ A. Juss.) Mull. Arg., the primary source of natural rubber. The diverse fungal pathogens responsible for these diseases lead to significant economic losses in rubber plantations, threatening the global rubber supply. This review provides a comprehensive examination of major fungal diseases affecting rubber plants, focusing on their identification, scientific analysis, and management strategies. Conventional methods for pathogen identification, while valuable, often lack the efficiency and precision required for effective disease control. Recent advancements in molecular diagnostics, genomics, and biotechnology have greatly improved the accuracy and speed of pathogen detection, enabling more targeted and sustainable management practices. Integrated pest management, mainly through the use of disease-tolerant clones, is emerging as a viable alternative to chemical fungicides. Although chemical fungicides remain the most widely used solution due to their accessibility and effectiveness, their environmental impact and potential for resistance necessitate a shift toward sustainable practices. While biofungicides offer a more environmentally friendly option and are already in use, they are currently limited to small-scale plantations and have yet to be adopted for large-scale production. Efforts to develop resistant clones through molecular studies and advanced cloning techniques are ongoing, representing the most sustainable approach for combating fungal diseases in rubber plantations. By synthesizing current knowledge and advancements, this review underscores the pressing challenges in managing fungal diseases and the opportunities presented by innovative technologies. The findings aim to guide future research and promote the development of efficient, sustainable strategies for maintaining rubber crop health, ensuring consistent production, and safeguarding the economic stability of the rubber industry.
Utiliization of Natural Compound from Pegagan (Centella asiatica (L.) Urb.) and Their Potential Role in the Health Sector Dewi, Ratna Lestyana; Yuniati, Ratna; Yasman
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.10558

Abstract

Centella asiatica, also known as Gotu Kola, is a medicinal plant widely used in traditional medicine. This abstract discusses its various health benefits supported by scientific research, including wound healing, cognitive enhancement, and anxiety reduction. It also explores its potential in treating skin conditions, venous insufficiency, and improving circulation. Further research is needed to fully understand its mechanisms of action and optimize its therapeutic applications. Centella asiatica (C. asiatica), commonly known as Gotu Kola, is a herbaceous plant extensively used in traditional Asian medicine for centuries. This review summarizes the scientifically validated health benefits of C. asiatica based on available research, primarily from Scopus-indexed publications. The plant's bioactive compounds, including triterpenoid saponins (asiaticoside, madecassoside, asiatic acid, and madecassic acid), contribute to its diverse pharmacological activities. C. asiatica exhibits significant wound healing properties by promoting collagen synthesis, angiogenesis, and fibroblast proliferation. Furthermore, studies suggest its potential in enhancing cognitive function, reducing anxiety, and improving memory. Its efficacy in treating skin conditions like eczema and psoriasis, as well as venous insufficiency and microcirculation, has also been reported. While promising, further research is needed to elucidate the precise mechanisms of action, determine optimal dosages, and explore potential synergistic effects with other therapeutic agents for various health conditions. This review aims to provide a comprehensive overview of the evidence-based health benefits of C. asiatica, highlighting its potential as a valuable natural remedy in modern medicine.
Implementasi Tempat Sampah Bambu Sebagai Solusi Pengelolaan Sampah Ramah Lingkungan Di Desa Pemepek, Kecamatan Pringgarata, Lombok Tengah Rohani; Karyawan, I Dewa Made Alit; Hasyim; Suteja, I Wayan; Negara, I Dewa Jaya; Yuniati, Ratna; Sideman, IAO Suwati; Salehudin; Saidah, Humairo; Yasa, I Wayan; Widianty, Desi
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13407

Abstract

Salah satu Desa di Lombok Tengah yang berada di Kecamatan Pringgarata adalah Desa Pemepek. Desa ini memiliki potensi wisata alam yang indah dan sangat menarik jika dilihat dari sisi keasrian ataupun keasliaan alam Desa Pemepek. Namun hal tersebut tidak luput dari permasalahan sampah terutama sampah plastik. Hingga kini belum ada aturan yang memuat tentang pembuangan sampah plastik. Akibatnya, banyak sekali sampah plastik yang tampak berserakan di pinggir jalan bahkan di beberapa tempat wisata yang seharusnya memiliki nilai estetika tinggi dan menarik bagi wisatawan, tapi jadi malah kelihatan kumuh dan kurang bisa menarik simpati wisatawan karena adanya sampah plastik. Oleh karena itu, program pengabdian ini bertujuan untuk melakukan sosialisasi dalam menyelesaikan bersama persoalan desa Pemepek yakni keberadaan sampah-sampah yang mengganggu nilai estetika tempat wisata tersebut. Tim pengabdian dibantu oleh mahasiswa KKN PMD Universitas Mataram Desa Pemepek mengadakan program kerja pembuatan tempat sampah dari bambu, yang diharapkan dapat mengedukasi masyarakat lokal dan wisatawan mengenai pentingnya pembuangan sampah pada tempat yang tepat untuk pelestarian alam sehingga dapat menciptakan suasana desa dan tempat wisata menjadi bersih, indah, asri dan sehat. Salah satu cara yang dilakukan adalah dengan membuat tempat sampah dari Bambu sehingga bisa digunakan untuk tempat pembuangan sampah.
Co-Authors Ainusyifa, Fathia AKIHO YOKOTA Ayunda, Ajenk damayanti, dini Damayanti, Margaretta Elsa Desi Widianty Dewi, Ratna Lestyana Didy Sopandie Eko Baroto Walujo Handayani, Windri Hasyim Hidayati, Eisya Hanina Humairoh Saidah I Wayan Suteja I Wayan Yasa, I Wayan Jumari Jumari, Jumari Karyawan, I Dewa Made Alit KINYA AKASHI Negara, I Dewa Jaya Prihantini, Nining Betawati Retno Lestari Rohani Salehudin Sideman, IAO Suwati Suharsono Suharsono UTUT WIDYASTUTI Wisnu Wardhana Yasman