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All Journal AL KAUNIYAH Jurnal Biodjati BIOEDUSCIENCE Jurnal Pengabdian Magister Pendidikan IPA Makara Journal of Science
Yuniati, Ratna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Materials Science & Nanotechnology

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Journal : Makara Journal of Science

 1 
PENGARUH KONSENTRASI MEDIUM EKSTRAK TAUGE (MET) TERHADAP PERTUMBUHAN Scenedesmus ISOLAT SUBANG Prihantini, Nining Betawati; Damayanti, Dini; Yuniati, Ratna
Makara Journal of Science Vol. 11, No. 1
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The effect of Tauge Extract Medium (TEM) concentration to the growth of Subang isolated Scenedesmus. Research on the effect of several concentration of Tauge Extract Medium (TEM) to Scenedesmus Meyen cell densities was conducted. The research was an experimental study with complete random design with 8 treatments, i.e. 1%, 2%, 3%, 4%, 5%, 6% TEM, aqua bides, and Bold Basal Medium (BBM). The results showed that TEM concentration effected cell densities of Scenedesmus. At peak condition, the highest cell density (3.981.071 sel/mL) was occurred in 4% at the day of seven, and the lowest cell density (87.096 sel/mL) in aqua bides at the day of three. Kruskal-Wallis test showed that there were differences between the mean of cell densities on all treatment media. Multiple comparison test showed that mean of cell numbers of Scenedesmus differed among treatments (p value>0,05) between aqua bides with 1%, 2%, 3%, 4%, 5%, 6%, and BBM.
PENAPISAN GALUR KEDELAI Glycine max (L.) Merrill TOLERAN TERHADAP NaCl UNTUK PENANAMAN DI LAHAN SALIN Yuniati, Ratna
Makara Journal of Science Vol. 8, No. 1
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Screening of Soybean Cultivars Glycine max (L.) Merrill under Sodium Chloride Stress Condition. Salinity is one of the most serious and widespread agricultural problems resulting in losses of yield. Generally, as land is more intensively cultivated, the salinity problem becomes more severe. A high concentration of NaCl greatly reduces growth of both the shoot and the root. One strategy available to cope with saline soil is to choose salt-tolerance crops or to select salt-tolerance cultivars within a crop. Experiments were conducted to asses the performance of ten cultivars soybean (Glycine max [L.] Merrill) to salt stress at germination and seedling stages. Salinity treatments were begun by adding 70, 80, 90, and 100 mM NaCl to the basal nutrient solution. According to germination percentage, fresh weight/dry weight ratios, and the percentage of dead apical buds we suggest that Wilis, Malabar and Sindoro were tolerant lines, Genjah Jepang, Lokan, and Tidar were moderate and the sensitive lines were Lumut, Yellow Biloxy, Si Cinang and Sriyono.
ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; Suharsono, Suharsono
Makara Journal of Science Vol. 15, No. 2
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Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.
Co-Authors AKIHO YOKOTA Ayunda, Ajenk damayanti, dini Damayanti, Margaretta Elsa Desi Widianty Didy Sopandie Eko Baroto Walujo Handayani, Windri Hasyim Hidayati, Eisya Hanina Humairoh Saidah I Wayan Suteja I Wayan Yasa, I Wayan Jumari Jumari, Jumari Karyawan, I Dewa Made Alit KINYA AKASHI Negara, I Dewa Jaya Prihantini, Nining Betawati Retno Lestari Rohani Salehudin Sideman, IAO Suwati Suharsono Suharsono UTUT WIDYASTUTI Wisnu Wardhana