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Immunohistochemistry Evaluation of TGF-Î²1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus) Soetjahjo, Bintang; Hidayat, Mohammad; Sujuti, Hidayat; Fibrianto, Yuda
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Currently, umbilical cord mesenchymal stem cells have the potential to be used as treatment options for any cartilage lesion. This research aimed to evaluate the effects of conditioned medium from umbilical cord mesenchymal stem cells (UC-MSC) on damaged cartilage through the expression of proteins TGF-Î²1, SOX-9, type II collagen and aggrecan, which are known to be related to chondrogenesis. UC-MSC were isolated from 19-days-pregnant Wistar mice and were cultured using the standard procedure to obtain 80% confluence. Subsequently, the culture was confirmed through a microscopic examination that was driven to be an embryoid body to obtain a pre-condition medium. This research utilized 3-month-old male Wistar mice and was categorized into 6 groups (3 control and 3 treatment groups). Each animal had surgery performed to create a femur condyle cartilage defect. The treatment groups were administered a dose of stem cells at 1 mL/kg. Next, immunohistochemical (IHC) staining was performed to examine the expression of TGF-Î²1, SOX-9, type II collagen and aggrecan in the 2nd, 3rd, and 4th month of evaluation. The results were analyzed statistically using ANOVA test. For each of the treatment groups, there was increased expression (p < 0.05) in all proteins TGF-Î²1, SOX-9, type II collagen and aggrecan when compared with control groups at the 2nd, 3rd, and 4th month of evaluation. Pre-conditioned medium from UC-MSC potentially increases the expression of TGF-Î²1, SOX-9, type II collagen and aggrecan in the damaged cartilage of Wistar mice.

Secondary Knee Osteoarthritis with Medial Instability and Tibial Defect due to Chronic Abscess Treated by Total Knee Arthroplasty with Metal Augmentation - Case Report Soetjahjo, Bintang; Hancoro, Udi Herunefi; Ermawan, Rieva; Saputra, Rhyan Darma; Akbar, Hillan
Indonesian Journal of Medicine Vol. 6 No. 2 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background:Knee osteoarthritis (OA), known as degenerative joint disease, is typically the result of wear and tear and progressive loss of articular cartilage, most common in elderly women and men. Knee OA is a leading cause of disability and increase with an aging and overweight population. Total Knee Arthroplasty (TKA) is a common surgical treatment of OA knee that highly cost-effective procedure. Stable placement of components is difficult and thus it presents technical challenges for the surgeon but various options are available for managing these defects.Case Report: A 58 years-old housewife came to our clinic due to chronic left knee pain with associated instability since two years ago and getting worse in last three months. She already had knee joint supplementation injections and physical therapy but no improvements. Based on our clinical and radiological findings, our assessment was secondary left knee osteo-arthritis with medial instability and tibial defect due to chronic abscess. Patient underwent TKA to correct this condition. A revised implant was used to close the defect in the medial part of the tibia and soft tissue reconstruction procedure was used to correct the instability.Result: On the first postoperative month, the patient underwent rehabilitation and physical therapy to strengthen her lower limb muscles. On the second month, she started to walk using walking assisted device. On the ninth months follow-up, the patient was able to walk normally without assisted device, the pain disappeared and the symptoms of knee instability were reduced.Conclusion:Bone defects are commonly encountered during TKA, which still has no standard treatment to cure. In this case, the use of metal augmentation with revision implant give satisfactory results with good clinical outcome.Keywords: Osteoarthritis, Total Knee Arthro¬plasty, knee instability, tibial defect, metal augmentationCorrespondence:Bintang Soetjahjo. Dr. Moewardi Hospital. Jl. Kolonel Sutarto 132, Jebres, Surakarta, Central Java 57126, Indonesia. Mobile: +628122987359. Email: bjortho@yahoo.comIndonesian Journal of Medicine (2021), 06(02): 168-176https://doi.org/10.26911/theijmed.2021.06.02.06

Masalah Perioperatif pada Kasus Emergensi Ortopedik Selama Pandemi Covid-19: Laporan Kasus Amputasi Darurat Pasien Suspek Covid-19 Soetjahjo, Bintang; Hancoro, Udi Herunefi; Ermawan, Rieva; Saputra, Rhyan Darma; Nugroho, Bagus Jati; Abdulhamid, Muhammad
SENADA : Semangat Nasional Dalam Mengabdi Vol. 1 No. 3 (2021): SENADA: Semangat Nasional Dalam Mengabdi
Publisher : Perkumpulan Dosen Periset Indonesia

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Penyakit Coronavirus 2019 (COVID-19), yang disebabkan oleh severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), telah menyebar ke seluruh dunia. Selama epidemi COVID-19 yang sedang berlangsung, banyak rumah sakit yang menunda sebagian besar operasi elektif. Namun, beberapa operasi darurat, terutama untuk pasien trauma, tidak bisa dihindari. Untuk pasien yang dicurigai atau dikonfirmasi COVID-19, protokol standar yang terkait persiapan pra operasi, manajemen intraoperatif, dan pengawasan pasca operasi harus diterapkan untuk menghindari infeksi nosokomial dan memastikan keselamatan pasien juga tenaga Kesehatan yang terlibat. Dalam artikel ini kami melaporkan kasus seorang pasien berusia 65 tahun dengan crush injury tungkai bawah dan suspek covid-19. Pasien direncanakan untuk menjalani CITO debridemen dan amputasi di atas lutut. Artikel ini kemudian membahas pertimbangan perioperatif dan teknis yang penting untuk menangani pasien COVID-19 yang membutuhkan perawatan darurat, tanpa mengesampingkan hasil klinis sembari memastikan keselamatan staf yang ada

Mesenchymal Stem Cells Secretome and Osteoarthritis: A State of The Art Soetjahjo, Bintang; Utomo, Dwikora Novembri
The Hip and Knee Journal Vol 3, No 2 (2022): August
Publisher : Indonesian Hip and Knee Society (IHKS)

No agreed cure or procedure can prevent the damage caused by OA. Current treatments such as physiotherapy, anti-inflammatory drugs, and viscous supplements are symptomatic and are aimed solely at pain relief. Mesenchymal stem cells, on the other hand, refer to a population of cells with immunomodulatory and homing properties. Recent studies have shown that stem cells produce secretomes with tissue regeneration, immunomodulation, anti-inflammatory, and anti-apoptotic capacities.Secretomes in conditioned media produced by mesenchymal stem cells can stimulate the repair of cartilage defects. There are many evidence from many studies both in vitro and in vivo which provide potential results. The improvement can be seen from the morphology, in the form of thickening of the cartilage, an increase in the number of chondrocyte cells, regular joint surfaces, and histologically it can be seen that there is an improvement in the joint cartilage matrix. Likewise, histological studies using immunohistochemistry also showed an increase in the expression of TGF-β, SOX-9, aggrecan, and type II collagen which is a pathway for the formation of hyaline cartilage repair. Not only improvement, other studies have also shown that the secretome can provide a protective effect on joint cartilage by increasing the expression of the COL2A1 gene which functions to form collagen matrix components and reduces apoptosis in chondrocyte cells.Secretomes produced by mesenchymal stem cells can stimulate the repair of cartilage damage, anti-inflammatory, immunomodulator, angiogenesis, and anti-apoptotic abilities from their cytokines and extra vesicles contains miRNA. MSCs secretomes are more stable and provide simpler manufacturing than MSCs themselves.

Functional Outcome Evaluation of Grade III Open Tibial Fracture Treated by External Fixation as Definitive Treatment Soetjahjo, Bintang; Ermawan, Rieva; Saputra, Rhyan Darma; Hancoro, Udi Herunefi; Nugroho, Bagus Jati; Anugra, Jiva Yori
Indonesian Journal of Medicine Vol. 6 No. 4 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Tibial shaft fractures including open tibial fractures grade III are one of the most common fractures of long bones. There are many methods of conservative and operative treatment, one of them is external fixation. External fixation is more common used temporary in polytraumatized patients with tibial shaft fractures. The study was undertaken to see if the patient can be treated with external fixation as the definitive treatment and evaluate the functional outcome after the treatment.Subjects and Method: A retrospective review of a prospectively-collected database was performed. Data was taken from the Orthopaedic Department of RSUD Dr. Moewardi Hospital patients’ database. The study included all patients who underwent grade III open tibial fracture treatment from May 2018 to May 2019. A total of 8 patients who were included in our study were a patient with open tibial fractures grade III planned for external fixaton as definitive treatment. They were evaluated radiographically and clinically to determine the union rate. The data were reported descriptively.Results: External fixator time ranged in this study around 240 days. In this study, there were a few patients whose progress were remain unknown due to loss of contact. Fractures studied 5 out of 8, no patient were union after 8 months of external fixator used. 4 out of 8 patients were reported non-union after 8 months based on their radiological examination (50%) and 1 out of 8 patients were reported has been performed Removal of External Fixation (ROEF) after 5 months of treatment. The rest of the 3 patients’ results were remain unknown.Conclusion: Open tibial fractures grade III of the leg can be managed with use of external fixator as a definitive treatment. However, the use of external fixation does not provide maximum results in grade III A tibial fractures.Keywords: open tibial fractures, external fixation, union, malunionCorrespondence: Bintang Soetjahjo. Dr. Moewardi General Hospital. Jl. Kolonel Sutarto 132, Jebres, Surakarta, Central Java, Indonesia. 57126. Email: bjortho@yahoo.com. Indonesian Journal of Medicine (2021), 06(04): 452-459https://doi.org/10.26911/theijmed.2021.06.04.11

Convalescent Plasma Treatment for Moderate to Critical Ill COVID-19 Patients – A Safe but Futile Treatment: A Non-Randomized Comparative Study Aphridasari, Jatu; Soetjahjo, Bintang; Joko, Agus; Sidharta, Rina; Harsini, Harsini; Arifin, Arifin; Permana, Septian Adi; Saraswati, Kunti; Adhiputri, Artrien; Marwanta, Sri; Laqif, Abdurahman; Danuaji, Rivan
Indonesian Journal of Medicine Vol. 8 No. 4 (2023)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/theijmed.2023.8.4.620

Background: In a number of cases of viral infection, convalescent plasma therapy has been effective. Reportedly, the use of convalescent plasma as a therapy for COVID-19 patients with severe and life-threatening disorders is beneficial at this time. This study aims to assess the effectiveness and safety of convalescent plasma transfusions in hospitalized COVID-19 patients. Subjects and Method: This was a clinical trial employing a non-randomized comparative study. A historical control group (21 samples) and convalescent plasma transfusions (21 samples) was selected consecutively from hospitalized COVID-19 patients between May 6th, 2020, and May 6th, 2021 at Dr. Moewardi General Hospital. We assessed and quantified viral clearance in the laboratory. Statistical analysis is performed in SPSS version 20.0. Results: Plasma was taken from fifteen convalescent donors. In the plasma convalescent treatment group, there was a statistically significant difference between outcome and severity degree (p = 0.005). In addition, there was a substantial discrepancy between the result group and the control group (p 0.005). Significant differences in post-treatment NLR between the control and treatment groups (p 0.005). In addition, there were statistically significant differences between the control and treatment groups in post-treatment hsCRP levels (p 0.005). In addition, there were statistically significant differences (p 0.005) between all groups' inflammatory markers and outcomes. Conclusion: Using convalescent plasma to treat patients with COVID-19 is a rather safe practice. Our analysis demonstrated that the administration of convalescent plasma did not enhance survival or clinical outcomes for COVID-19 patients with moderate to severe disease. Keywords: COVID-19, convalescent therapy, critical ill

BIBLIOMETRIC ANALYSIS OF PATIENT OUTCOMES AND SATISFACTION IN ROBOTIC-ASSISTED HIP AND KNEE JOINT REPLACEMENT SURGERY Soetjahjo, Bintang; Santoso, Asep; Mercy, Grendi; Adriansyah, Denny; Angeline, Agiona; Sadewa, Daffa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13395

Robotic-assisted hip and knee arthroplasty has gained popularity in orthopedic surgery due to its precision, faster recovery, and improved alignment, potentially enhancing patient outcomes. Despite mixed and heterogeneous evidence, the demand for these procedures continues to rise. Therefore, bibliometric analysis is essential to direct research towards patient satisfaction and generate more reliable and significant evidence proving the true benefits of robotic surgery for patients, rather than merely technical success. This study employed bibliometric analysis to explore trends and evaluate publications on patient outcomes and satisfaction in robotic-assisted hip and knee surgery using the Scopus database. The analysis identified core concepts, trends, and research connections, providing insights into emerging areas in the field. The bibliometric analysis of 324 documents from 2007 to 2025 reveals a significant increase in research, focusing on surgical precision, rehabilitation, and long-term effectiveness. While countries like the US, UK, and China are leading contributors, there is a notable lack of similar research in ASEAN countries, particularly Indonesia. Future research should address gaps in areas like surgical revision, patient perception, and improving patient-reported outcomes.

BIBLIOMETRIC ANALYSIS OF PATIENT OUTCOMES AND SATISFACTION IN ROBOTIC-ASSISTED HIP AND KNEE JOINT REPLACEMENT SURGERY Soetjahjo, Bintang; Santoso, Asep; Mercy, Grendi; Adriansyah, Denny; Angeline, Agiona; Sadewa, Daffa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13395

Robotic-assisted hip and knee arthroplasty has gained popularity in orthopedic surgery due to its precision, faster recovery, and improved alignment, potentially enhancing patient outcomes. Despite mixed and heterogeneous evidence, the demand for these procedures continues to rise. Therefore, bibliometric analysis is essential to direct research towards patient satisfaction and generate more reliable and significant evidence proving the true benefits of robotic surgery for patients, rather than merely technical success. This study employed bibliometric analysis to explore trends and evaluate publications on patient outcomes and satisfaction in robotic-assisted hip and knee surgery using the Scopus database. The analysis identified core concepts, trends, and research connections, providing insights into emerging areas in the field. The bibliometric analysis of 324 documents from 2007 to 2025 reveals a significant increase in research, focusing on surgical precision, rehabilitation, and long-term effectiveness. While countries like the US, UK, and China are leading contributors, there is a notable lack of similar research in ASEAN countries, particularly Indonesia. Future research should address gaps in areas like surgical revision, patient perception, and improving patient-reported outcomes.

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