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Profiling Rosmarinic Acid and Sinensetin Content of Orthosiphon aristatus. from Three Different Locations with Variety Ethanol Concentration Ramadhani, Novia Putri; Murti, Yosi Bayu; Gani, Andayana Puspitasari
Journal of Food and Pharmaceutical Sciences Vol 12, No 2 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.13625

Orthosiphon aristatus is a well-known medicinal plant acknowledged for its therapeutic effect in treating urinary tract diseases, hypertension, diabetes mellitus, and arthritis. It is widely used as an ingredient in herbal medicine and distributed throughout the world, including China, Europe and Indonesia. Sinensetin are normally used as chemical marker to evaluate and control the quality of O. aristatus. However, in 2021, the European Medicines Agency changed the marker to rosmarinic acid. In order to determine the levels of rosmarinic acid and sinensetin in O. aristatus as well as the correlation between the two compounds, this study used high-performance liquid chromatography (HPLC) with a UV detector to analyze O. aristatus extract from three distinct locations with four different ethanol concentrations (96%, 75%, 50%, and 25%). Results showed that higher levels of rosmarinic acid was obtained in extractions using binary. Meanwhile, higher sinensetin content in O. aristatus were obtained by 96% ethanol (Klaten) and hydro-ethanol (Sleman, Bantul). In addition, this study discovered that the sinensetin levels had an inverse correlation with rosmarinic acid levels. It can be concluded that the rosmarinic acid and sinensetin contents are influenced by growing locations and ethanol concentrations.

The relationship analysis of the traditional medicine knowledge level and self-medication knowledge level on the practice the use of traditional medicine at the University of Darussalam Gontor Islamanda, Cania Sofyan; Gani, Andayana Puspitasari; Endarti, Dwi
Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy Vol. 8 No. 2 (2024): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v8i2.12026

The use of traditional medicine aims to provide preventive, promotive, curative, and rehabilitative measures against a disease. Self-medication is one of the curative health efforts that is often carried out by the community to prevent the disease they are suffering from, as an act of selecting and using medication without a doctor's prescription to treat disorders and symptoms of disease. This research aims to increase understanding, knowledge, and practice of traditional medicine self-medication. This type of research is observational-quantitative, using a cross-sectional approach, which is a research design to study correlation techniques between risk factors and the factors that influence them. Data collection used questionnaires distributed directly with a sample size of 481 respondents. Descriptive analysis was used to describe the entire research sample data in the form of sociodemographic data, level of knowledge, self-medication, and practice of using traditional medicine. The inferential analysis used is the Spearman rank correlation test to see the relationship between variables. The results of the research show that there is a significant relationship between the level of knowledge of traditional medicine and the practice of using traditional medicine by respondents of (p value=0.001) and r value = 0.146 and there is a significant relationship between knowledge of self-medication and the practice of using traditional medicine of (p value= 0.005) and r value = 0.128 so this research is in the same direction. Keywords: knowledge, practice, self-medication, traditional medicine

Profiling Rosmarinic Acid and Sinensetin Content of Orthosiphon aristatus. from Three Different Locations with Variety Ethanol Concentration Ramadhani, Novia Putri; Murti, Yosi Bayu; Gani, Andayana Puspitasari
Journal of Food and Pharmaceutical Sciences Vol 12, No 2 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.13625

Orthosiphon aristatus is a well-known medicinal plant acknowledged for its therapeutic effect in treating urinary tract diseases, hypertension, diabetes mellitus, and arthritis. It is widely used as an ingredient in herbal medicine and distributed throughout the world, including China, Europe and Indonesia. Sinensetin are normally used as chemical marker to evaluate and control the quality of O. aristatus. However, in 2021, the European Medicines Agency changed the marker to rosmarinic acid. In order to determine the levels of rosmarinic acid and sinensetin in O. aristatus as well as the correlation between the two compounds, this study used high-performance liquid chromatography (HPLC) with a UV detector to analyze O. aristatus extract from three distinct locations with four different ethanol concentrations (96%, 75%, 50%, and 25%). Results showed that higher levels of rosmarinic acid was obtained in extractions using binary. Meanwhile, higher sinensetin content in O. aristatus were obtained by 96% ethanol (Klaten) and hydro-ethanol (Sleman, Bantul). In addition, this study discovered that the sinensetin levels had an inverse correlation with rosmarinic acid levels. It can be concluded that the rosmarinic acid and sinensetin contents are influenced by growing locations and ethanol concentrations.

Optimasi Penggunaan High Shear Mixer pada Pembuatan Fraksi Alkaloid dari Daun Awar-awar (Ficus septica) dengan Desain Faktorial Kusnanto, Chelvin Ari; Gani, Andayana Puspitasari; Wahyuono, Subagus; Fakhrudin, Nanang
Jurnal Kefarmasian Indonesia VOLUME 11, NOMOR 2, AGUSTUS 2021
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v11i2.4874

Awar-awar (Ficus septica) is an Indonesian anticancer plant that contains alkaloids as the active compound. The n-hexane insoluble fraction (FTLH) is alkaloid-containing fraction obtained from the ethanolic extract of F. septica leaves (EEDFS) by fractionation using n-hexane. High shear mixer (HSM) can be used to optimize the separation processes, including fractionation. The application of HSM in the fractionation of EEDFS is affected by fractionation duration, amount of solvent per gram extract, and strirring strength. Thus, these parameters must be optimized to obtain the optimum condition for the production of FTLH with the highest alkaloid content. This study aimed to optimize the production of FTLH using HSM with factorial designs. The single factor experimental design was employed to determine the influence of the individual variable on the alkaloid content and to define the optimum range value of each variable. A full factorial design was used to determine the presence of interaction among the factors and to determine the optimal fractionation condition. The results showed that all investigated factors independently affected alkaloid contents. The solvent volume of 14-18 mL per gram extract, the fractionation duration of 2.5-5.0 minutes, and the stirring strength of 3000 rpm are the optimal range value of each factor. The optimal conditions were solvent volume of 14.0-14.4 mL/gram extract, fractionation duration of 2.5-2.7 minutes, and strirring strength at 3000 rpm. At the optimal condition, the total alkaloid content in the FTLH reached 0.1466% or 1.3 times higher than the extract (0.1128%).

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