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Study of Tissue Cyst Formation Time of Toxoplasma gondii in Mice M. Hanafiah; Wisnu Nurcahyo; Sumartono S
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.511 KB) | DOI: 10.21157/j.ked.hewan.v1i2.3132

Abstract

 The purpose of the research was to study a tissue cyst formation time Toxoplasma gondiiexperimentally. A number of 84 mice were divided randomly into four groups. Each group consisted of 21mice. The mice of the group I were infected with 101, II with 102 and III with 10 tachyzoites respectivelyintraperitoneally, whereas the group IV as a control (not infected with tachyzoites). All infected micewere treated with sulfadiazine, 15 mg/mouse per oral diluted in drinking water, for 5 days. On first untiltwenty first day after treatment one mouse of each group was necropsied. Liver, lymph, kidney, lung,heart, brain, or diaphragm muscle were then taken for histological preparations. Data on tissue cystformation time was analysed descriptively. The research revealed that innoculation with tachyzoites 103cyst could be found on day 14th after infection of liver, 102 cyst was found on the 6 day of liver, in day7th in heart and brain on day 10th of after infection, 103 cyst was found on day 4th inheart and brain in day 7thth in liver, day 6 after infection, while in the control dosage there is no formation similar to cyst found.Keywords: cyst, tissue, T. gondii, mice th1
IN VITRO DEVELOPMENT OF Ascaridia galli EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Wida Wahidah Mubarokah; Kurniasih Kurniasih; Wisnu Nurcahyo; Joko Prastowo
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.993 KB) | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
Integrated Community-Based Conservation of Olive Ridley Sea Turtles in Response to Coastal Abrasion Yudhi Ratna Nugraheni; Raden Wisnu Nurcahyo; Dwi Priyowidodo; Labib Abdillah; Artina Prastiwi; Vika Ichsania Ninditya; Edi Yulianto; Dhiannisa Ismoyo Putri
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol. 10 No. 2 (2026): May 2026
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29062/engagement.v10i2.2211

Abstract

Background: Coastal abrasion and human activities have significantly degraded the nesting habitats of the olive ridley sea turtle (Lepidochelys olivacea). Purpose of the Study: This community service program aimed to save sea turtles through conservation activities, including nest relocation, hatching, and releasing hatchlings through a community-based collaborative approach. Methods: This community service focused on relocating olive ridley sea turtle nests threatened by coastal abrasion and developing hatching facilities. Through hatchling releases and conservation education, the program increased local community participation and promoted sustainable eco-tourism. Results: The program successfully relocated 28 nests containing 3,005 eggs, increasing hatching success from <50% to 64.7%. Monthly hatchling releases and educational activities increased community awareness and strengthened their role in eco-based sea turtle conservation