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Antibacterial, Antifungal And Subchronic Toxicity Test Of Ficus Deltoidea Jack Leaves Extract Apriasari, Maharani Laillyza; Adhiya, Geyanina Melda; Wasiaturrahmah, Yusrinie; Utami, Juliyatin Putri; Firdaus, I Wayan Arya Krishnawan; Pramitha, Selviana Rizky
Odonto : Dental Journal Vol 12, No 1 (2025): April 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.1.19-28

Background: Ficus deltoidea Jack is a plant that is often used as herbs medicine because of secondary metabolites such as alkaloids, phenolics, flavonoids, and steroids. This study aims to determine the activity of antibacterial, antifungal and toxicity effect of Ficus deltoidea Jack leaves (FDLE) extract. Method: Pure experimental study with a posttest-only design with a control group design. The in vitro test by dilution methods using Staphylococcus aureus and Candida albicans. The toxicity test used 16 male Rattus novergicus divided into four group consist a control group and three treatment group given FDLE doses with 1,250, 2,500, and 3,750 mg/kgBW. The treatment applied 2x1 ml FDLE every 24 hours orally for 28 days. Result: The MIC value and the MBC value of FDLE againts S. aureus were 8% and 10%, respectively. While, MIC and MBC value on C. albicans has similar value about 25%. The mean results of kidney toxicity test were still in the normal range. There was a significant difference in the results of the Kruskall Wallis test for urea (p<0.05), while there was no significant difference in the results of urea and creatinine level (p>0.05). Conclusion: There was antibacterial and antifungal efficacy of FDLE. Also, there was no toxic effect of giving FDLE at doses of 1,250, 2,500, and 3,750 mg/kgBW was showed on urea and creatinine normal level.

Antibacterial, Antifungal And Subchronic Toxicity Test Of Ficus Deltoidea Jack Leaves Extract Apriasari, Maharani Laillyza; Adhiya, Geyanina Melda; Wasiaturrahmah, Yusrinie; Utami, Juliyatin Putri; Firdaus, I Wayan Arya Krishnawan; Pramitha, Selviana Rizky
Odonto : Dental Journal Vol 12, No 1 (2025): April 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.1.19-28

Background: Ficus deltoidea Jack is a plant that is often used as herbs medicine because of secondary metabolites such as alkaloids, phenolics, flavonoids, and steroids. This study aims to determine the activity of antibacterial, antifungal and toxicity effect of Ficus deltoidea Jack leaves (FDLE) extract. Method: Pure experimental study with a posttest-only design with a control group design. The in vitro test by dilution methods using Staphylococcus aureus and Candida albicans. The toxicity test used 16 male Rattus novergicus divided into four group consist a control group and three treatment group given FDLE doses with 1,250, 2,500, and 3,750 mg/kgBW. The treatment applied 2x1 ml FDLE every 24 hours orally for 28 days. Result: The MIC value and the MBC value of FDLE againts S. aureus were 8% and 10%, respectively. While, MIC and MBC value on C. albicans has similar value about 25%. The mean results of kidney toxicity test were still in the normal range. There was a significant difference in the results of the Kruskall Wallis test for urea (p<0.05), while there was no significant difference in the results of urea and creatinine level (p>0.05). Conclusion: There was antibacterial and antifungal efficacy of FDLE. Also, there was no toxic effect of giving FDLE at doses of 1,250, 2,500, and 3,750 mg/kgBW was showed on urea and creatinine normal level.

Toxicity test of Stenochlaena palustris extract based on kidney histopathology examination Apriasari, Maharani Laillyza; Sari, Tiara Intan Permata; Firdaus, I Wayan Arya Krishnawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p178-183

Background: Kalimantan's people consume Stenochlaena palustris leaf extract as food and for traditional medicine. The bioactive components of Stenochlaena palustris leaf extract are flavonoids, alkaloids, saponins, and tannins. An in vitro study shows that the leaf extract has no toxic effect, so it can be used as an alternative drug in oral health, such as in mouthwashes or topical ulcer drugs. Purpose: This study aims to analyze the toxic effects of Stenochlaena palustris leaf extract based on the bleeding and lesions resulting from necrosis in kidney by using histopathology examination. Methods: The Stenochlaena palustris leaves were extracted using 95% ethanol and then given to male Wistar strain (Rattus norvegicus) with a 2,000, 2,500, and 3,000 mg/kg/body weight two times a day for fourteen days. The kidneys were collected and subjected to histopathology examination. Results: There are higher bleeding and necrosis lesion rates in the 2,500 and 3,000 mg/kg/body weight of Stenochlaena palustris leaves extract group compared to the control and 2,000 mg/kg/body weight of Stenochlaena palustris leaves extract group (p<0.05). Conclusion: Stenochlaena palustris leaf extract showed no toxic effect at doses of 2,000 mg/kg/body weight.

ANTIBACTERIAL EFFECT OF KELAKAI LEAF EXTRACT (STENOCHLAENA PALUSTRIS (BURM) BEDD.) FOR INHIBITING ENTEROCOCCUS FAECALIS Firdaus, I Wayan Arya Krishnawan; Dewi, Nurdiana; Fuady, Ridwan Ichshalul; Apriasari, Maharani Laillyza
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Root canal treatment is a stage of treating pulp infection by removing the necrotic tissue and eliminating microorganisms. Inadequate sterilization cause persistent root canal bacteria, including Enterococcus faecalis. The irrigation solution that has become the gold standard in root canal treatment is Sodium hypochlorite but it has some weaknesses. Kelakai leaf extract can be an alternative root canal irrigation because it has minimal side effects and antibacterial compounds such as flavonoid, saponin, alkaloid, and tannin.Methods: This research was a true experimental laboratory with posttest only and control group design. The research using 5 treatment groups with 3 replications, so that total sample was 15 samples. Group 1-4 were kelakai leaf extract concentrations of 25%, 50%, 75%, 100% and group 5 was Sodium hypochlorite 2,5%. The parameter measured was the diameter of the inhibition zone (mm) formed on MHA.Results: One Way Anova and Post Hoc LSD test results showed a significant difference between each treatment group of kelakai leaf extract compared with Sodium hypochlorite 2,5%. Kelakai leaf extract 25%, 50%, 75%, 100% concentrations, and Sodium hypochlorite 2,5% had an average inhibition zone diameter which were 9.47 mm, 14.64 mm, 17.91 mm, 21.24 mm, and 23.27 mm.Conclusion: Kelakai leaf extract concentrations of 25%, 50%, 75%, and 100% had inhibitory activity against Enterococcus faecalis but had not been equivalent to Sodium hypochlorite 2,5%.

ANTIBACTERIAL EFFECT OF KELAKAI LEAF EXTRACT (STENOCHLAENA PALUSTRIS (BURM) BEDD.) FOR INHIBITING ENTEROCOCCUS FAECALIS Firdaus, I Wayan Arya Krishnawan; Dewi, Nurdiana; Fuady, Ridwan Ichshalul; Apriasari, Maharani Laillyza
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.110-118

Background: Root canal treatment is a stage of treating pulp infection by removing the necrotic tissue and eliminating microorganisms. Inadequate sterilization cause persistent root canal bacteria, including Enterococcus faecalis. The irrigation solution that has become the gold standard in root canal treatment is Sodium hypochlorite but it has some weaknesses. Kelakai leaf extract can be an alternative root canal irrigation because it has minimal side effects and antibacterial compounds such as flavonoid, saponin, alkaloid, and tannin.Methods: This research was a true experimental laboratory with posttest only and control group design. The research using 5 treatment groups with 3 replications, so that total sample was 15 samples. Group 1-4 were kelakai leaf extract concentrations of 25%, 50%, 75%, 100% and group 5 was Sodium hypochlorite 2,5%. The parameter measured was the diameter of the inhibition zone (mm) formed on MHA.Results: One Way Anova and Post Hoc LSD test results showed a significant difference between each treatment group of kelakai leaf extract compared with Sodium hypochlorite 2,5%. Kelakai leaf extract 25%, 50%, 75%, 100% concentrations, and Sodium hypochlorite 2,5% had an average inhibition zone diameter which were 9.47 mm, 14.64 mm, 17.91 mm, 21.24 mm, and 23.27 mm.Conclusion: Kelakai leaf extract concentrations of 25%, 50%, 75%, and 100% had inhibitory activity against Enterococcus faecalis but had not been equivalent to Sodium hypochlorite 2,5%.

In Silico Model of Musa acuminata Stem as an Anticancer on Oral Squamous Cell Carcinoma against EGFR Rachmayani, Nevrila Dewi; Carabelly, Amy Nindia; Firdaus, I Wayan Arya Krishnawan; Apriasari, Maharani Laillyza; Utami, Juliyatin Putri
Journal of Dentistry Indonesia Vol. 33, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Oral squamous cell carcinoma (OSCC) is characterized by overexpression of the epidermal growth factor receptor (EGFR). Citral, eucalyptol, and caffeic acid phenethyl ester (CAPE), which are derived from Musa acuminata, have shown anticancer activity. This study evaluated the potential of citral, eucalyptol, and CAPE from Musa acuminata as anticancer agents for OSCC by targeting EGFR using an in silico approach. Methods: Potential biological activity was predicted using Prediction of Activity Spectra for Substances (PASS) Online. Molecular docking was conducted with AutoDock Vina, and BIOVIA Discovery Studio was used to analyze binding affinity, interaction types, and bond distances between the compounds and active-site residues of EGFR. Results: CAPE showed the strongest binding affinity (-8.6 kcal/mol), whereas citral and eucalyptol each showed a binding affinity of -5.4 kcal/mol. CAPE formed a conventional hydrogen bond with CYS775 at 2.65 Å and hydrophobic interactions with PHE856, LEU777, and MET766, through π-alkyl and π-π T-shaped interactions. These interaction distances were within the effective range of <3.5 Å, indicating a stable ligand-protein complex. Conclusions: These in silico findings suggest that CAPE derived from Musa acuminata is a promising anticancer candidate for OSCC through its interaction with EGFR.

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