Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Sylva Lestari Jurnal Bioteknologi & Biosains Indonesia
Fauzan, Yusuf Sigit Ahmad
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO Fauzan, Yusuf Sigit Ahmad; Supriyanto, .; Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.189 KB) | DOI: 10.29122/jbbi.v4i2.2540

Abstract

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017
KAJIAN ELONGASI PADA TANAMAN IN VITRO GAHARU (Aquilaria beccariana van Tiegh) Fauzan, Yusuf Sigit Ahmad; Sandra, Edhi; Mulyono, Daru
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (737.168 KB) | DOI: 10.29122/jbbi.v2i2.511

Abstract

The population density of natural agarwood (Aquilaria beccariana) in Indonesia decreased to less than one tree per hectare. Efforts have been carried out on ex situ conservation of agarwood despite facing many obstacles. In vitro propagation is one alternative to speed up the recovery of natural agarwood populations. The purpose of this study was to obtain optimal elongation media for in vitro culture with addition of auxin and cytokinin, namely IBA, BAP and kinetin. The results showed that the best auxin-cytokinin combination was IBA 0.1 mg/L and BAP 0.05 mg/L. This combination increased the height and number of segments of A. beccariana with an average height of 1.64 cm and average number of sections of 6.40. It is suggested that this combination of IBA and BAP was the most effective compared to the other treatments. In addition, the combination of IBA 0 mg/L and BAP 0.03 mg/L gave rise to the best response to increase the number of shoots with an average of 1.91 shoots.Keywords: Aquilaria beccariana, shoot, elongation, auxin, cytokinin ABSTRAKKepadatan populasi gaharu (Aquilaria beccariana) alam di Indonesia kurang dari satu pohon per hektar. Upaya pelestarian gaharu ex situ telah banyak dilakukan tetapi masih banyak kendala. Perbanyakan gaharu in vitro merupakan salah satu cara alternatif untuk mempercepat pemulihan populasi gaharu alam. Tujuan penelitian ini adalah untuk memperoleh media elongasi yang optimal pada kultur in vitro gaharu dengan penambahan kombinasi zat pengatur tumbuh auksin dan sitokinin. Pada penelitian ini digunakan auksin IBA, serta sitokinin BAP dan Kinetin. Hasil penelitian elongasi diperoleh kombinasi auksin dan sitokinin terbaik yaitu, IBA 0,1 mg/L dan BAP 0,05 mg/L. Kombinasi ini meningkatkan tinggi dan jumlah ruas Aquilaria beccariana dengan tinggi rata-rata sebesar 1,64 cm dan jumlah ruas rata-rata sebesar 6,40 ruas. Pada kombinasi dan taraf ini diduga mekanisme kerja IBA dan BAP paling efektif dibanding perlakuan yang lain. Sedangkan kombinasi IBA 0 mg/L dan BAP 0,03 mg/L memberikan respon terbaik terhadap peningkatan jumlah tunas dengan rata-rata sebanyak 1,91 tunas.Kata Kunci: Aquilaria beccariana, tunas, elongasi, auksin, sitokinin
Genetic Improvement of Araucaria cunninghamii for Enhanced Industrial Pulp Production Pamungkas, Bayu; Setiadi, Dedi; Baskorowati, Liliana; Mashudi; Pudjiono, Sugeng; Nurtjahjaningsih, Ida Luh Gede; Susanto, Mudji; Hendrati, Rina Laksmi; Sumardi; Fauzan, Yusuf Sigit Ahmad; Wirabuana, Pandu Yudha Adi Putera
Jurnal Sylva Lestari Vol. 13 No. 3 (2025): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v13i3.1163

Abstract

Araucaria cunninghamii has significant potential as a long-fiber pulpwood source in Indonesia; however, its utilization remains limited due to its dependence on imported raw materials. This study presents the results of a first-generation progeny trial designed to evaluate genetic variation and identify superior genotypes for future breeding programs. This study was conducted in Bondowoso, East Java; the experimental was included 80 families from six provenances (Fakfak, Jayapura, Serui, Wamena, Manokwari, and Queensland) and designed by a Randomized Complete Block Design (RCBD); comprised of 6 provenances, 80 families, 4 blocks, 4 trees per plot with a spacing of 4 m ´ 2 m. After six years, the survival rates ranged from 98.30% to 99.61%, indicating a strong potential for adaptability. Significant differences were observed among families for height, diameter, and volume growth traits. Heritability estimates for height, diameter, and volume at the individual level were 0.30, 0.25, and 0.27, respectively, and 0.48, 0.45, and 0.47 at the family level. Strong genetic correlations were found between height and diameter (r = 0.99), suggesting the potential for indirect selection through breeding. These findings highlight the genetic potential of A. cunninghamii for sustainable pulp production and inform future breeding strategies that focus on growth, adaptability, and wood quality. Keywords: Araucaria cunninghamii, genetic variation, progeny test, pulpwood, heritability, provenance trial
ROOT INDUCTION ON THE SHOOTS OF RUBBER TREE (Hevea brasiliensis) CLONE PB 260 THROUGH EX VITRO TECHNIQUE Fauzan, Yusuf Sigit Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1740

Abstract

Rubber tree (Hevea brasiliensis) is a crucial plantation commodity for Indonesia's national development. However, the country's rubber productivity lags behind that of other rubber-producing nations. To address this, propagating rubber plants from superior clones through ex-vitro techniques becomes essential.. This study aimed to determine the optimal concentrations of IBA and NAA for root induction in rubber tree shoot explants. A factorial complete randomized design was employed, with the first factor being IBA concentration (0, 100, 200, 300, 400, and 500 ppm) and the second factor being NAA concentration (0, 50, 100, and 150 ppm). The results revealed that the percentage of surviving explants after induction with IBA and NAA did not significantly differ. The rooted explants were obtained using the following treatments: IBA 200 ppm and NAA 0 ppm, IBA 200 ppm and NAA 50 ppm, IBA 300 ppm and NAA ppm, and IBA 400 ppm and NAA 0 ppm. Various concentrations of IBA and NAA did not significantly affect root formation in rubber tree shoot explants.
Co-Authors . Supriyanto bayu pamungkas Daru Mulyono Dedi Setiadi Edhi Sandra Liliana Baskorowati Mashudi Mudji Susanto Nurtjahjaningsih, Ida Luh Gede Rina Laksmi Hendrati Sugeng Pudjiono, Sugeng Sumardi Teuku Tajuddin Wirabuana, Pandu Yudha Adi Putera