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The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra Ridho, Muhammad Arif; Martinius; Fadli; Liswarni, Yenny; Najmi, Lailatul; Trisno, Jumsu
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.183-187

Tanaman mentimun varietas lokal Padang merupakan tanaman sayuran unggulan bagi petani perkotaan karena umur panen yang pendek. Hasil survei menemukan adanya gejala bercak kuning dan klorosis dengan tulang daun hijau. Gejala ini mirip dengan infeksi Papaya ring spot virus (PRSV) yang menginfeksi mentimun di Jawa dan Zucchini yellow mosaic virus (ZYMV) yang menginfeksi mentimun pada umumnya. Deteksi virus dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan primer spesifik PRSV dan primer universal Potyvirus, perunutan dan analisis DNA. RT-PCR menggunakan primer spesifik PRSV DNA target tidak menghasilkan pita DNA, namun pita DNA dengan ukuran ±320 pb berhasil teramplifikasi dengan primer universal Potyvirus. Analisis sikuen nukleotida menunjukkan bahwa similaritas virus tertinggi sebesar 93.8% dan 93.5%, berturut-turut dengan ZYMV isolat Singapura dan India. Infeksi ZYMV merupakan kejadian pertama pada tanaman mentimun di Padang, Sumatera Barat.

Uji Konsentrasi Ekstrak Air Daun Lantana camara Linnaeus terhadap Mortalitas dan Perkembangan Spodoptera exigua Hubner (Lepidoptera: Noctuidae): english Liswarni, yenny; Sari, Nicha Puspita; Hamid, Hasmiandy
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 1 (2018): June 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.1.28-36.2018

Spodoptera exigua Hubner is one of the major pests which attack onion crop. Botanical pesticides can be used as an alternative that is cheap, easy and environmentally friendly in controlling these pests. Lantana camara Linn is a plant that has the potential as a botanical pesticide. This study aimed at obtaining the best concentration of water extract of leaves of L. camara which was active on mortality, reduced feeding activity and development of S. exigua in the laboratory. The experiment was conducted using a Completely Randomized Design (CRD), with 6 treatments and 5 replications. The concentration was used a 0%, 2%, 4%, 6%, 8% and 10%. The treatment was done by the application through the larval feed. Parameters observed were larval mortality, reduced feeding activity, duration of larval development, the percentage of pupal, weight of pupal and percentage of adult formed. The results showed that the concentration of water extract of leaves of L. camara as insecticide with LC50 value was 5.02%. Water extract of leaves of L. camara also decreased feeding activity, inhibit development of larval, pupal and adult formed.

Aktifitas Ekstrak Metanol Kulit Buah Manggis (Garcinia mangostana L) terhadap Crocidolomia pavonana Fabricius (Lepidoptera: Crambidae): English Arneti, Arneti; Liswarni, Yenny; Sinaga, Ismi Fadhilah
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 1 (2018): June 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.1.37-43.2018

Crocidolomia pavonana is an important pest of Brassicaceous which can be controlled by botanical insecticide. The objective of this study was to determine activity of the mangosteen peel fruit (G. mangostana) methanol extract on mortality and development of C. pavonana. The research was conducted at laboratory. The design used was a completely randomized design with four level of methanol extract of the mangosteen peel fruit on larva of 2nd instar. The extract was tested to larvae by a leaf-residue feeding method without selection. The parameters were mortality, antifeedant activity, developmental time, and numbers of pupae C. pavonana formed. The results showed that methanol extract of the mangosteen peel fruit was less effective in controlling C.pavonana. The highest concentration of extract (0.5%) caused mortality 72%, antifeedant activity 55,70%, extended larval developmental time and inhibited numbers of pupae formed of 30,66%.

Aktivitas Antagonistik Jamur yang Berasosiasi dengan Nematoda Bengkak Akar (Meloidogyne spp.) pada Rizosfer Tanaman Tomat: English Winarto, Winarto; Trizelia, Trizelia; Liswarni, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 2 (2018): December 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.2.78-86.2018

The purpose of experiment were to know antagonistic activity of fungi to root-knot nematode (Meloidogyne spp.) in rhizosphere of tomato. The experiment consisted of isolation, test of antagonistic activity and identification of fungi that carried out at Phytopathology Laboratory of Universitas Andalas. Soil samples were taken from rhizosphere of healthy tomato in Alahan Panjang, Solok, and Agam. The results indicated that there were seven antagonistic fungi to Meloidogyne spp., namely: Paecilomyces sp., Penicillium, sp., Aspergillus sp., Fusarium sp., Gliocladium sp. Trichoderma sp., and Chaetomium sp.

Ketahanan Beberapa Varietas Padi Lokal Sumatera Barat terhadap Virus Penyebab Penyakit Tungro: english Liswarni, Yenny; Martinius, Martinius; Nurbailis, Nurbailis
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 2 (2019): December 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.2.93-99.2019

Tungro disease is one of the obstacles to increasing national rice production. The use of resistant varieties is a component to control the virus that is environmentally friendly and easily accepted because it does not require additional costs. This study aimed to determine the resistance of nine rice varieties commonly planted by farmers against the tungro. The study was conducted in a greenhouse, and the source of the tungro virus inoculum was taken from the Lintau area of ??West Sumatra Province. The results showed that of the nine varieties tested, IR 42 was resistant to the tungro attack, seven others were classified as moderate, and one variety was classified as vulnerable. The moderate resistant varieties were Anak Daro, Cisokan, Cantik Manih, Saganggam Panuah, Junjuang, Kuriek Kusuik, and Caredek Merah. In contrast, the variety that classified as vulnerable was Batang Sungkai due to the symptoms of attack more clearly than on other varieties, the fastest of a period of symptoms appear (10.15 days), the highest disease incidence (80.62%) with a disease index of 7.65.

Efektivitas Ekstrak Daun Pepaya secara Invitro terhadap Colletotrichum gloeosporioides Penyebab Penyakit Antraknosa pada Tanaman Cabai: Effectiveness of papaya leaf extract in vitro against Colletotrichum gloeosporioides, the pathogen of the anthracnose disease on red pepper arneti, Arneti; Liswarni, Yenny; Edriwilya, Rifa
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 4 No. 1 (2020): June 2020
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.4.1.1-10.2020

Papaya leaf (Carica papaya Linnaeus) is one of the sources of botanical fungicides that is known to have an ability in inhibiting Colletotrichum gloeosporioides growth. The aim of the study was to determine the effectiveness of papaya leaves extract to suppress of C. gloeosporioides growth that caused anthracnose disease of red pepper (Capsicum annuum Linnaeus) in vitro assay. The study was done in a Completely Randomized Design (CRD) with five concentration levels of papaya leaves extract and control (1%, 2%, 3%, 4%, 5%, and control). The parameters were colony growth, colony area, wet weight, dry weight, and conidia number (per ml of suspension). The data obtained were analyzed by ANOVA and LSD tests at 5% significance level. The results showed that the application of papaya leaves extract with different concentrations could suppress the growth of C. gloeosporioides, the pathogen of anthracnose disease in chili. The higher the concentration of papaya leaves extract given, the higher its effectiveness in suppressing the growth of C. gloeosporioides. Application at the 5% concentration was the most effective inhibiting the conidia formation (82.5%), followed by inhibiting the expansion of the colony (64.04%), reducing wet weight (45.16%) and dry weight (54.16%). Keywords: Anthracnose, botanical fungicides, Capsicum annuum, concentration, growth inhibitor

Daya Predasi dan Tanggap Fungsional Kumbang Unta (Ophionea nigrofasciata) pada Beberapa Kepadatan Wereng Batang Coklat (Nilaparvata lugens): Predation Rate and Functional Response of Camel Groundbeetle (Ophionea nigrofasciata) on Several Densities of Brown Planthopper (Nilaparvata lugens) Nasral, Tre Julia; Syahrawati, My; Liswarni, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 4 No. 1 (2020): June 2020
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.4.1.11-20.2020

Camel groundbeetle (Ophionea nigrofasciata Schmidt-Goble 1846) or CGB is reported as one of predators of brown planthopper (Nilaparvata lugens Stal 1854) or BPH. The difference in BPH densities is assumed to affect the predation rate of the CGB. This study aimed to determine the predation rate of CGB on BPH at different densities. This experiment used a completely randomized design (CRD) consisting of six treatments and five replications. The treatment consisted of predation of one CGB at several BPH densities (5, 10, 20, 30, 40, and 50 individuals). BPH used was second-third instar of nymphs, and the CGB used was female. The results showed that the predation rate of CGB increased with increasing BPH density with functional response types classified as type I (Linear). The highest predation occurred at 50 density (23.6 individuals or 47.2% of BPH provided). The difference in density of BPH did not affect the bodyweight gain of CGB and tended to decrease due to different prey-seeking behavior. Keywords: Nillaparvata lugens, Ophionea nigrofasciata, predation, predator

KETEPATAN WAKTU APLIKASI Paecilomyces lilacinus DALAM MENGENDALIKAN NEMATODA BENGKAK AKAR (Meloidogyne spp.) PADA TANAMAN TOMAT: Time Suitability of Application of Paecilomyces lilacinus in Controlling Root-knot Nematodes (Meloidogyne spp.) on Tomato Winarto, Winarto; Darnetty, Darnetty; Liswarni, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 4 No. 1 (2020): June 2020
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.4.1.38-44.2020

Root-knot nematodes (Meloidogyne spp.) have been reported to be one of the primary pathogens that decreased tomato production in Indonesia. Biological control of root-knot nematodes by using parasitic fungus as like as Paecilomyces lilacinus is still limited. An effective application of parasitic fungi could be successful by managing a suitability application time. The study aimed to determine the suitability of the application time of the P.lilacinus in controlling root-knot nematodes on tomato. The study was conducted in farmers' land that was infected by root-knot nematodes. The experiment was done in a randomized block design with applying P.lilacinus isolates on 12, 8, and 4 days before planting, planting time, and 4, 8, 12 days after planting. All treatments were repeated four times. The application of P. lilacinus onto tomato root at planting time was better at suppressing the development of root-knot nematode compared to applications made before or after planting. P. lilacinus was able to suppress the number of root-knot (66.08%), the number of egg groups (77.33%), the number of eggs (26.79%), and the number of nematodes in the soil (82.20%). Keywords: Application time, Meloidogyne spp., Paecilomyces lilacinus, tomato

Antagonism of Endophytic Bacteria and the Consortium Against Culvularia oryzae Bugnic: Kemampuan Antagonis Bakteri Endofit dan Konsorsiumnya terhadap Pertumbuhan Jamur Culvularia oryzae Bugnic Resti, Zurai; Martinius, Martinius; Liswarni, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 6 No. 1 (2022): June 2022
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.6.1.34-42.2022

Endophytic bacteria have several advantages, including controlling plant pathogens and increasing plant growth. As a biological control, endophytic bacteria can suppress the development of plant pathogenic fungi. This study aimed to obtain endophytic bacteria and the consortium of endophytic bacteria that can inhibit the growth of C. oryzae. The study used a Completely Randomized Design (CRD) for studying the ability of endophytic bacteria using eight isolates: Bacillus sp HI, Bacillus sp SJI, B. cereus P14, B. cereus Se07, Serratia marcescens strain JB1E2, S. marces-cens strain JB3, S. marcescens strain ULG1E2 and S. marcescens strain ULG1E4, with three replications. For Studying the ability of endophytic bacteria consortium using six endophytic bacteria consortia and control with three replications. Observations were made on the inhibition growth of C. oryzae. The results showed that all endophytic bacteria and the endophytic bacteria consortium could inhibit C. oryzae, with inhibition of 58.50% – 75.00% and 38.00% – 77.00%. Endophytic bacteria which can significantly suppress the growth of C. oryzae were B. cereus Se07, Bacillus sp HI and Bacillus sp SJI with inhibition of 75.00%, 70.50%, and 70.00%. The endophytic bacteria consortium that had a high ability to suppress the growth of C. oryzae was the consortium of B (S. marcescens strain ULG1E4, S. marcescens strain JB1E3), D (Bacillus sp SJI, Bacillus sp HI, S. marcescenns JB1E3), and G (Bacillus sp SJI, S. marcescens strain ULG1E4), with inhibition of 77.00%, 72.33%, and 71.33%, respectively.

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