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All Journal Cropsaver : Journal of Plant Protection JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Akta Agrosia Journal of Applied Agricultural Science and Technology Open Science and Technology
Darnetty, Darnetty
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Environmental Science Mechanical Engineering

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Antagonistic Test of Endophytic Bacteria Against Fusarium oxysporum f.sp. cepae Causes of Moler Disease on Shallots Wihayyu, Afri; Resti, Zurai; Sulyanti, Eri; Darnetty, Darnetty; Khairul, Ujang
CROPSAVER Vol 7, No 1 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i1.54351

Abstract

Fusarium oxysporum f.sp. cepae (FOCe) is a pathogenic fungus causing moler disease in shallots. One method of controlling this disease is by using endophytic bacteria. Endophytic bacteria are bacteria that live in plant tissues without causing disease symptoms. This research aims to obtain the most effective endophytic bacteria in inhibiting the growth of the pathogenic fungus FOCe. The study employs a Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications each. The treatments include Bacillus sp. HI, Bacillus sp. SJI, Bacillus cereus P14, Bacillus cereus Se07, Bacillus subtilis, Serratia marcescens JB1E3, Serratia marcescens ULG1E4, and control. Tests conducted include the antibiosis test of endophytic bacterial cells using the dual culture method and the secondary metabolite test using media poisoning method. The observed parameters include inhibition zone, effectiveness of secondary metabolites, fresh weight, and dry weight of the FOCe fungus. All endophytic bacteria were capable of inhibiting the growth of FOCe. The most effective endophytic bacteria in inhibiting the growth of FOCe were found to be Serratia marcescens ULG1E4 and Serratia marcescens JB1E3. In the antibiosis test of endophytic bacterial cells, Serratia marcescens ULG1E4 exhibited an inhibition zone of 64.44%, while Serratia marcescens JB1E3 showed an inhibition zone of 61.11%. In the secondary metabolite compound test, the effectiveness values for Serratia marcescens ULG1E4 and Serratia marcescens JB1E3 were 95.31% and 95.03%, respectively.
Bagaimana Trichoderma Meningkatkan Enzim Pertahanan pada Tanaman Pangan: Pandangan Saat Ini dan Perspektif Masa Depan di Asia Albana, Hasan; Nurbailis, Nurbailis; Darnetty, Darnetty
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.109-122.2024

Abstract

Food crops are plants grown for consumption by humans and animals. These crops face threats from both abiotic and biotic factors. Therefore, environmentally friendly alternative controls are needed, such as enhancing plant resistance using Trichoderma spp. This systematic literature review provides information on the effects of Trichoderma spp. application on plant physiology, particularly focusing on defense enzymes in food crops in the Asian region. Articles on the effects of Trichoderma application on food crop defense enzymes in Asia were retrieved from PubMed and Scopus. The literature search yielded 3,022 articles from these databases. Four criteria were established for study inclusion: i) studies involving Trichoderma spp.; ii) studies using food crops as the subject; iii) studies conducted in Asia; and iv) studies evaluating at least one defense enzyme activity. India has conducted the most research on this topic, with a total of 26 studies. Across 43 reviewed articles, 100 isolates were identified. The most frequently used Trichoderma species was T. harzianum, and the most common inoculation method was seed treatment, reported in 25 articles. Additionally, 50% of the articles conducted their studies in controlled environments. Trichoderma spp., known for enhancing plant resistance, also have the ability to increase certain physiological aspects of plants, such as defense enzyme activity. More research on changes in plant enzymes due to Trichoderma application is recommended, especially in field conditions, to assess its effectiveness in practical, real-world settings.
Biological Control Stem Rot Diseases (Sclerotium Rolfsii) on Peanut (Arachis hypogaea L.) using Arbuscular Mycorrhizal Fungi (AMF) Indigenous Swandi, Fradilla; Sulyanti, Eri; Darnetty, Darnetty
Akta Agrosia Vol 24 No 1 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Arbuscular Mycorrhizal Fungi (AMF) are known to have potential as biological agents controlling plant pathogens. This study aims to obtain indigenous AMF isolates that can suppress the attack of Sclerotium rolfsii which causes stem rot disease in peanut. The method used is an experimental method with a Completely Randomized Design with 5 treatments, namely A : AMF Glomus sp-3 + S. rolfsii; B: AMF Acaulospora sp + S. rolfsii; C: AMF Gigaspora sp + S. rolfsii; D: Combined AMF Glomus sp-3, Acaulospora sp, and Gigaspora sp + S. rolfsii; E: Without AMF + S. rolfsii (Control). Each treatment was repeated 5 times. The data were analyzed using Analysis of Variance (ANOVA) using the Statistix 8 program and the Least Significance Different (LSD) test at a 5% significance level. The results showed that the isolates of Acaulospora sp and Gigaspora sp were able to increase the resistance of peanut plants to stem rot disease (suppressing the incidence and severity of the disease) reaching 100%. Keywords: Arbuscular Mycorrhizal Fungi, indigenous, salicylic acid, Sclerotium rolfsii.
Ability of Beauveria bassiana to suppress Colletotrichum truncatum and increase the growth of chili plants (Capsicum annuum) Yanty, Dini Puspita; Trizelia, Trizelia; Darnetty, Darnetty; Trisno, Jumsu
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 1 (2024): June 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.1.42-52.2024

Abstract

Anthracnose is a disease caused by Colletotrichum sp., which can reduce the quality and yield of red chili by 45-60%. This study aimed to determine the ability of different Beauveria bassiana isolates to control anthracnose in chili. The research employed a Randomized Block Design (RBD) consisting of 6 treatments and 5 replications. The treatments included a control and 5 different B. bassiana isolates (PD114, TD312, WS, BbKo, KT2B21). The observed variables included incubation period, disease incidence and severity, plant height, number of leaves, flowering time, and chili fruit weight. The results showed that the application of different B. bassiana isolates have effectively inhibited the development of Colletotrichum truncatum, which caused anthracnose in chili. B. bassiana has demonstrated the ability to prolong the incubation period of C. truncatum (5.40 days) and reduce the percentage of disease incidence (16%). Furthermore, the application of B. bassiana increased the height of chili plants (40.40 cm) and the number of leaves (32 leaves), accelerated the flowering time (42.80 days), and boosted chili production (178.25 g). The WS and TD312 isolates revealed the most significant results. Although the severity of the disease was not impacted, the 2 isolates provided notable agronomic benefits to the chili plants.
Bagaimana Trichoderma Meningkatkan Enzim Pertahanan pada Tanaman Pangan: Pandangan Saat Ini dan Perspektif Masa Depan di Asia Albana, Hasan; Nurbailis, Nurbailis; Darnetty, Darnetty
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.109-122.2024

Abstract

Food crops are plants grown for consumption by humans and animals. These crops face threats from both abiotic and biotic factors. Therefore, environmentally friendly alternative controls, such as enhancing plant resistance using Trichoderma spp, are needed. This systematic literature review provides information on the effects of Trichoderma spp. application on plant physiology, particularly focusing on defense enzymes in food crops in the Asian region. Articles on the effects of Trichoderma application on food crop defense enzymes in Asia were retrieved from PubMed and Scopus. The literature search yielded 3,022 articles from these databases. Four criteria were established for study inclusion: i) studies involving Trichoderma spp.; ii) studies using food crops as the subject; iii) studies conducted in Asia; and iv) studies evaluating at least one defense enzyme activity. In this systemic review, we found at least 100 isolates of Trichoderma spp. that can increase plant resistance, as indicated by the increase of plant defense enzymes in Asia. The studies were mostly reported in India and China. Rice is the most frequently studied crop. The most frequently used is T. harzianum. Meanwhile, the defense enzymes tested are peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, chitinase, and β-1,3-glucanase. The most commonly used inoculation method is seed treatment in a controlled environment such as a greenhouse. More research on changes in plant enzymes due to Trichoderma spp. application is recommended to assess its effectiveness in practical, real-world settings, especially in field conditions.
Biocontrol of Moler Diseases (Fusarium oxysporum f. sp capae (Snyder and Hans)) on Shallot with Endophytic Bacteria Resti , Zurai; Darnetty, Darnetty; Khairul , Ujang; Reflin , Reflin; Siregar , Sri Lestari Kurnia; Tores, Farah Nabila
Journal of Applied Agricultural Science and Technology Vol. 8 No. 3 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i3.243

Abstract

Fusarium Basal Root (FBR) disease locally known as "moler", caused by Fusarium oxysporum f.sp cepae (FOCe), poses a significant threat to Indonesian shallot cultivation, leading to yield losses of approximately 50%. Therefore, this research aims to identify endophytic bacteria with optimal capabilities to combat FOCe infection while improving shallot growth and yield. Randomized Completely Block Design (RCBD) consisting of seven treatments was used, including six different strains of endophytic bacteria (Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, Bacillus sp. SJI, Serratia marcescens ULG1E2, and Serratia marcescens JB1E3) and a control, each treatment was replicated three times, and three plant units in each replication. The introduction of endophytic bacteria was performed through the bulb soaking method before planting, while FOCe suspension was inoculated to shallot growing media 4 weeks after planting. Parameters Variables such as moler disease development, plant growth, and shallot bulb weight were monitored. The results showed that endophytic bacteria effectively suppressed moler disease and boosted shallot growth and yield compared to the control. Specifically, S. marcescens JB1E3 showed 52.25% effectiveness in reducing moler disease severity, while S. marcescens ULG1E2 increased shallot yield by 65.50%.
Endophytic Bacteria Consortia as Biocontrol Against Brown Spot Disease (Helminthosporium oryzae) and Growth Promotion in Rice Sulyanti, Eri; Resti, Zurai; Rahma, Haliatur; Darnetty, Darnetty; Khairul, Ujang; Nurhaliza, Nurhaliza; Oktaviani, Silvia
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.1-13.2025

Abstract

The brown spot disease (BSD) caused by Helminthosporium oryzae in rice can lead to yield losses of up to 45 %. Using endophytic bacterial consortia as a biological agent is one of the most potential control methods for resisting the disease. This study aimed to obtain an endophytic bacterial consortium with the best efficacy in controlling BSD and its ability to enhance rice plant growth. Statistical analysis in this study was made using Analysis of Variance (ANOVA) with Duncan’s Multiple Range Test (DMRT) in a randomized experimental design comprising five treatments with five replications. The treatments included: A (Serratia marcescens ULG1E4 + S. marcescens JB1E3), B (S. marcescens ULG1E4 + S. marcescens JB1E3 + Serratia marcescens JB1E2), C (Bacillus sp. SJI + Serratia marcescens ULG1E4), D (positive control), and E (negative control). Testing was conducted in planta, with the introduction of the bacterial consortium (10⁸ cells/mL) via seed and root soaking methods. Subsequently, the leaves of 30-day-old rice plants were inoculated with H. oryzae by spraying a conidial suspension of 10⁶ conidia/ml. Parameters observed included disease progression (incubation period, incidence, and severity), seedling growth metrics (height, leaf number, root length, fresh and dry biomass), and overall plant development (plant height, leaf number, and tiller count). The results demonstrated that the consortium of Serratia marcescens ULG1E4, Serratia marcescens JB1E3, and Serratia marcescens JB1E2 effectively controlled BSD, achieving a 53.69% reduction in disease severity and promoted rice plant growth, evidenced by a 30.63% increase in leaf number
Isolation and identification of arbuscular mycorrhizal fungi (amf) microscopically in the rhizosphere of peanut plants Swandi, Fradilla; Sulyanti, Eri; Darnetty, Darnetty
Open Science and Technology Vol. 3 No. 2 (2023): Open Science and Technology
Publisher : Research and Social Study Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33292/ost.v3i2.108

Abstract

Fungi Mikoriza Arbuskular (FMA) merupakan jamur yang bersimbiosis dengan perakaran tumbuhan tingkat tinggi. FMA dapat berperan sebagai agen biokontrol dan biofertilizer. Studi ini bertujuan mengetahui jenis-jenis FMA pada rhizosfer tanaman kacang tanah di Nagari Sawah Tangah, Kecamatan Pariangan, Kabupaten Tanah Datar dan sebagai tahapan awal dalam pemanfaatan FMA sebagai agen biokontrol dan biofertilizer. Sebanyak lima sampel tanah diambil menggunakan teknik purposive random sampling. Sampel tanah yang diperoleh disaring menggunakan teknik penyaringan basah. Identifikasi spora FMA dilakukan hingga tingkat genus berdasarkan karakteristik morfologi, yakni bentuk, warna, serta ornamentasi spora. Hasilnya menunjukkan bahwa jumlah spora FMA tertinggi pada rhizosfer tanaman kacang di Nagari Sawah Tangah, Kecamatan Pariangan, Kabupaten Tanah Datar adalah dari genus Glomus (3 tipe), yakni Glomus sp-1 sebanyak 15 spora, Glomus sp-2 sebanyak 12 spora, dan Glomus sp-3 sebanyak 74 spora (total 101 spora). Jumlah tertinggi kedua adalah dari genus Acaulospora (1 tipe) sebanyak 27 spora, sementara jumlah spora FMA terendah adalah dari genus Gigaspora (1 tipe) sebanyak 9 spora per 100 gr sampel tanah. Arbuscular Mycorrhizal Fungi (AMF) are fungi that have a symbiotic relationship with the roots of higher plants. AMF can act both as biocontrol agent and biofertilizer. This study aims to determine the types of AMF in the rhizosphere of peanut plants in Nagari Sawah Tangah, Pariangan Subdistrict, Tanah Datar Regency, as an initial stage in the application of AMF as a biocontrol agent and biofertilizer. Five soil samples were taken using a purposive random sampling technique. The soil samples obtained were then filtered using a wet filtration technique. Identification of AMF spores was carried out at the genus level based on their morphological characteristics, namely shape, color, and spore ornamentation. The results reveal that the highest number of AMF spores in the rhizosphere of peanut plants in Nagari Sawah Tangah, Pariangan Subdistrict, Tanah Datar Regency is from the genus Glomus (3 types), namely Glomus sp-1 with 15 spores, Glomus sp-2 with 12 spores, and Glomus sp-3 with 74 spores (a total of 101 spores). The second highest number is from the genus Acaulospora (1 type) with 27 spores, and the lowest number of AMF spores is from the genus Gigaspora (1 type) with 9 spores per 100 gr of soil sample.
Respon Beberapa Varietas Padi Terhadap Serangan Fusarium fujikuroi Penyebab Penyakit Bakanae Darnetty, Darnetty; Sulyanti, Eri
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 1 (2017): June 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.1.18-23.2017

Abstract

Fusarium fujikuroi is the cause of bakanae disease in rice plants. Level of bakanae disease attack on some rice cultivation land in West Sumatra, especially in Padang Pariaman district is quite high. One effort to suppress the development of F. fujikuroi is by planting resistant varieties. In this study, 15 rice varieties originating Sumatera Barat were used to be tested for their response to F. fujikuroi attack. The purpose of this study was to obtain the varieties with the low attack rate of F. fujikuroi. Parameters observed were number of dead seeds, number of dead seedlings, number of stunted seedlings, number of seeds showing symptoms of Bakanae and total infected seeds. The results of the study indicated that symptoms of F. fujikuroi attack varied which were dead seeds, stunting seedlings, dead seedslings and bakanae. The response of rice varieties to F. fujikuroi attack was different with characterized by different levels of infection rates. Low infection rates were found in Pessel varieties (2.0%), Batang sahalai (15.5%), Cisokan (17.0%), and Batang Lembang (20%). Other varieties showed a high percentage of F. fujikuroi attacks ranging from 20% to 54.5%.
Aktivitas Insektisida Ekstrak Air Campuran Buah Piper aduncum dan Daun Tephrosia vogelii terhadap Crocidolomia pavonana Fabricius (Lepidoptera: Crambidae): english Afriyanita, Afriyanita; Lina, Eka Candra; Darnetty, Darnetty
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 1 (2019): June 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.1.34-46.2019

Abstract

The botanical insecticide is alternative pest control that is feasible to be developed. The inflorescences extract of Piper aduncum and leaf extract of Tephrosia vogelii are known to have insecticidal activity. The study aimed to determine the effect of a mixture of water extract of P. aduncum inflorescences and T. vogelii leaves on C. pavonana. This test used a completely randomized design (CRD) consisting of 6 treatments (0.0, 2.5, 3.0, 3.5, 4.0 and 5.0%) and 5 replications. Observation parameters were larval mortality, antifeedant effect, larval development time, pupal development time, normal and abnormal pupae, and sex ratio. The mixture of water extract of P. aduncum inflorescences and T. vogelii leaves (2:1) at LC50 (3.19%) was antagonistic and at LC95 (6.07%) was additive. The mixture influenced larval mortality (81.0%), had antifeedant effect (84.5%) and prolonged larval development time 1.71 days on 2nd-3rd instars and 2.4 days on 2nd-4th instars compared to control, but there were no effect on pupal development time, number of abnormal pupae and sex ratio.
Co-Authors Afriyanita, Afriyanita ALBANA, HASAN Eka Candra Lina Haliatur Rahma, Haliatur Hermaleni, Utari Jumsu Trisno Khairul , Ujang Liswarni, Yenny NOVRI NELLY Nurbailis Nurhaliza, Nurhaliza Oktaviani, Silvia Putri, Desi Afrida Reflin , Reflin Resti , Zurai Ricar, Wahyu Siregar , Sri Lestari Kurnia Sulyanti, Eri Swandi, Fradilla Tores, Farah Nabila Trizelia . UJANG KHAIRUL Wihayyu, Afri Winarto Winarto Yanty, Dini Puspita Yunisman, Yunisman Zurai Resti