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Karakterisasi Genetik TRICHODERMA SPP Indigenus Rizosfir Pisang yang Berpotensi Pengendalian FUSARIUM OXYSPORUM F. SP. CUBENSE Penyebab Penyakit Layu Fusarium pada Pisang Nurbailis, Nurbailis; Martinius, Martinius
Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.116 KB)

Abstract

The objective of the research was to characterize the genetic variation of Trichoderma spp. Study of genetic characters used Random Amplified Polymorphic DNA-Polymerase Chain-reaction (RAPD-PCR) that amplicated with four primers (OPA 2, OPA 17, OPB- 05 and Primer 2).  Analysis result of genetic variation of Trichoderma spp make as dendrogram. The result of the research indicated that : 1) Trichoderma spp. had the great genetic variation, 2)Trichoderma isolate from the same region did not always have the same genetic profile, 3) S6 and T1 isolates were effective to inhibit the growth of Fusarium oxysporum f. Sp. cubense  than the others but  had a different genetic profile. Keywords : Trichoderma spp, Fusarium oxysporum f.sp.cubense, karakterisasi genetik
Pengaruh Kolonisasi Trichoderma spp. pada Akar Bibit Pisang terhadap Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense) Nurbailis, Nurbailis; Martinius, Martinius
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.5 KB) | DOI: 10.31258/jnat.13.3.220-225

Abstract

The purpose of the research was to obtain the superior Trichoderma that had ability to colonize root with the resultbeing effective to supress Fusarium wilt desease and promote banana seedling growth. This experiment consistedof 2 factors and 4 replications. The first factor was Trichoderma spp. namely : A. Trichoderma koningii strain S6sh(TK-S6sh), B Trichoderma viride strain T1sk (TV-T1sk) and Trichoderma harzianum strain S10sh (TH-S10sh). Thesecond factor was the kind of banana namely a.Cavendis, b. Barangan and c. Kepok. The observation werecolonization ability, Fusarium wilt desease development and the banana seedling growth. The result showed thatTV-T1sk was the best spesies to colonize all banana seedling root. The highest colonization in Barangan bananaseedling root reached 80%. Trichoderma colonization in banana seedling root could suppress Fusarium wilt diseasedevelopment and increase banana seedling weight. Higher ability of Trichoderma to colonize banana seedling rootcaused lower disease incidence of Fusarium wilt and greater biomass of banana seedling. Interaction betweenTV-T1Sk and Barangan banana was the best in colonization, so they were effective to suppress Fusarium wiltdesease and increase banana seedling biomass.
KAJIAN TEKNO EKONOMI PAKET TEKNOLOGI BAKTERI PERAKARAN PEMACU PERTUMBUHAN TANAMAN (BP3T) PUPUK KANDANG DAN NANO PESTISIDA SERAI WANGI PADA USAHA TANI KAKAO DI KABUPATEN LIMA PULUH KOTA Wahyuni, Sri; Rahma, Haliatur; Trisno, Jumsu; Martinius, Martinius; Noveriza, Rita; Reflin; Yuliani, Sri; Nusyirwan, Nusyirwan; Nefri, Jhon
Jurnal AGRISEP JURNAL AGRISEP VOL 19 NO 01 2020
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.533 KB) | DOI: 10.31186/jagrisep.19.1.68-82

Abstract

Plant Growth Promoting Rhizobacteria (PGPR) plus manure technology is designed to control the vascular streak dieback disease (VSD) which attacks cocoa plants, and improve the plant growth. This research aims to compare the cost of using conventional manure and PGPR plus manure technology. This research was designed in a descriptive quantitative approach using cencus data of 43 people who use PGPR plus manure in Kabupaten Limapuluh Kota. The result shows that the conventional fertilizer costs is more expensive than the PGPR plus manure formula. Therefore, it PGPR plus manure formula is recommended for the cacao farmers in Kabupaten Limapuluh Kota.
Aktivitas Insektisida Campuran Ekstrak Air Buah Piper aduncum L. (Piperaceae) dan Batang Cymbopogon ciratrus (Dc.) Stapf (Poaceae) Terhadap Larva Crocidolomia pavonana F. (Lepidoptera: Crambidae) Lina, Eka Candra; Supriadi, Adventus; Yunisman, Yunisman; Martinius, Martinius
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 1 (2017): June 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.1.34-41.2017

Abstract

Crocidolomia pavonana is an important pest in Brassicaceae. Botanical insecticides is pest control alternative which meet to eco-friendly manajement. This study aims to determine lethal concentration of single and mixture of water extract of Piper aduncum (Piperaceae) fruit and Cymbopogon cirtatus (Poaceae) stem against Crocidolomia pavonana larvae. The study was conducted in laboratory experiment through preliminary test and advance test using a completely randomized design (CRD). Observations were included to larval mortality, antifeedant effect, and duration of larval development. Bioassay was done against second instar larvae of C. pavonana using leaves immersion method during 48 hours. The results show that LC50 and LC95 value of mixture extract are 2.83% and 5.79% respectively. Based on index combination analysis, P. aduncum and C. citratus mixture extract were antagonistic at LC50 and additive at LC95 . Water extract of P. aduncum and C. citratus were relatively weak to influence feeding activity of C. pavonana larvae (41%). Mixture extract also extend larval development from second instar to third instar around 0.24 days and third instar to fourth instar around 0.97 days.
Keanekaragaman Serangga Air di Sawah Konvensional dan Organik di Kota Padang Hamid, Hasmiandy; Martinius, Martinius
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 2 (2017): Desember 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.2.68-78.2017

Abstract

Study on diversity of aquatic insects in rice is important to determine the condition of the rice fields. The purpose of this research was to study the community of aquatic insects in two difference cultivations (organic and conventional) and sub districts (Koto Tangah dan Pauh) in Padang City. Purposive random sampling method was used. Samples were collected by using water insect nets and water bottle traps. The results showed that the aquatic insects found in the study site consisted of 5 orders (Coleoptera, Diptera, Ephemeroptera, Hemiptera, Odonata), 16 families, 51 species and 3808 individuals. Aquatic insect populations in organic farming was higher (5 orders, 15 families, 48 species and 3089 individuals) than conventional (4 orders, 9 families, 20 species and 719 individuals). Aquatic insects found were acting as predators (3718 individuals), detrivors (7 individuals), and omnivors (83 individuals). The diversity indexes of aquatic insects on two locations were classified as medium but the diversity in organic cultivation was higher than conventional.
Patogenisitas Cendawan Entomopatogen dari Rizosfir Kacang Tanah (Arachis hypogaea Linnaeus) terhadap Hama Penggerek Polong Etiella zinckenella Treitschke (Lepidoptera: Pyralidae) Reflinaldon, Reflinaldon; Martinius, Martinius
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 2 (2017): Desember 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.2.79-87.2017

Abstract

The objective of this study was to get entomopathogenic fungi isolates of bean plant rhizosphere derived from Nagari Sawah Tangah, District Pariangan, Tanah Datar Regency and to determine their pathogenicity on pod borer E. zinckenella. Isolation method was performed by diluting the soil samples in a serial dilution into 10-3 and cultured in PDA media. Furthermore, the purification was based on the shape and color of the fungus colonies. A total of 16 isolates obtained at the initial stage were selected by testing them against the fifth instar larvae Tenebrio molitor. Results showed that only 4 isolates (STA 1, STA 2.2, STA 5, and STB 3.1) could be further tested against E. zinckenella pod borer using completely randomized design (CRD). The highest pathogenicity isolates was exhibited by STA 1 belonged to Metarhizium genera.
Kemampuan Isolat Rizobakteri sebagai Agens Antagonis Fusarium verticillioides Penyebab Penyakit Busuk Tongkol pada Tanaman Jagung (Zea mays Linnaeus), secara Invitro: english Martinius, Martinius; yunisman, yunisman; Harpani, Aisyah
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 1 (2018): June 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.1.43-53.2018

Abstract

Rhizobacteria is an alternative biocontrol of Fusarium verticillioides Sacc. Nirenberg caused ear rot on maize. The aim of this study was to screen rhizobacteria isolates that had ability as antagonist agent against F. verticillioides in vitro. Rhizobacteria was isolated from rhizosphere of healty maize from two villages of West Pasaman. Twenty six rhizobacteria isolates were obtained, then tested for hypersensitive reaction (HR), pectinase enzyme production, initial inhibitory activity, and hemolytic test. Two selected isolates: RBPas1 10-6 1 and RBPas2 10-5 2 could inhibit the growth of F. verticillioides. The inhibitory activity of both isolates were observed with dual culture method, using complete random design (CRD) with 2 treatments and 5 replications, furthermore physiological characterization was conducted. RBPas1 10-6 1 and RBPas2 10-5 2 isolates had the percentage inhibition of micelial growth: 46.16% and 31.30% respectively. Physiological characterization showed that RBPas1 10-6 1 was gram negative with baciliform cells, produced chitinase enzyme, siderophores and fluorescent. RBPas2 10-5 2 was gram positive with bacilliform cells, did not produce chitinase enzyme, siderophores and fluorescent.
Efektivitas Ekstrak Beberapa Tumbuhan untuk Pengendalian Keong Mas (Pomacea canaliculata Lamarck) pada Tanaman Padi Sawah: english Rusli, Rusdi; Martinius, Martinius; Marzuki, Dedi
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 1 (2019): June 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.1.1-9.2019

Abstract

Golden snail is an important pest on rice plants in Indonesia. The study purposed to obtain the most effective plant extracts to suppress the golden snail population. The study was conducted at the greenhouse of Faculty of Agriculture, Universitas Andalas, Padang from November to December 2018, using a Completely Randomized Design (CRD) with six treatments and four replications. The treatment was leaf extracts from several plants with a concentration of 10 g/l, that were god’s crown or mahkota dewa (Phaleria macrocarpa), noni or mengkudu (Morinda citrifolia), breadfruit or sukun (Artocarpus altilis), ti plant or andong (Cordyline fruticosa), mangkokan (Nothopanax scutellarium), and control. The parameters observed were mortality of golden snails, percentage of clumps attacked, and intensity of golden snail attacks. The results showed that there were three leaf extracts could suppress golden snail population significantly, ie. Mangkokan, andong and mahkota dewa. The mangkokan leaf extracts was most effective in controlling golden snails which caused mortality of golden snails to reach 100% within 24 hours of exposure so that they did not attack the clumps as a whole.
Aktivitas Air Rebusan Daun dari Beberapa Tumbuhan dalam Menekan Pertumbuhan Sclerotium rolfsii Sacc. Penyebab Busuk Batang pada Tanaman Kacang Tanah secara In Vitro: english Martinius, Martinius; Gani, Suardi; Ningsih, Juita Wilna
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 1 (2019): June 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.1.47-55.2019

Abstract

The leaves of some plants have been proved to suppress the development of plant diseases caused by pathogens. The study aimed to determine the water decoction of leaves to suppress the growth of S. rolfsii caused stem rot on peanuts in vitro. The study was conducted in the Laboratory of Phytopathology Departement of Plant Pest and Disease Faculty of Agriculture, Andalas University, from May-July 2015. The experimental design was a Randomized Complete Design (RCD) with seven treatments and six replications. The treatments were control, water decoction of Annona squamosa, Impatiens balsamina, Eclipta alba, Cymbopogon citratus and Tithonia diversifolia with concentration of 0,5% and fungicide with tebukonazol active ingredient at a concentration of 0,1%. Variables observed were macroscopic fungal culture, colony area, wet and dry weight of colonies. Data were analyzed by using ANOVA and DNMRT at a 5% of significance level.. The results showed that all water decoctions of plant leaves were able to suppress the growth of S. rolfsii. The most active of the water decoction was from T. diversifolia with suppression to colony area reached 77,32%, and wet and dry weight were 77,24% and 84,66%, respectively.
Ketahanan Beberapa Varietas Padi Lokal Sumatera Barat terhadap Virus Penyebab Penyakit Tungro: english Liswarni, Yenny; Martinius, Martinius; Nurbailis, Nurbailis
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 2 (2019): December 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.2.93-99.2019

Abstract

Tungro disease is one of the obstacles to increasing national rice production. The use of resistant varieties is a component to control the virus that is environmentally friendly and easily accepted because it does not require additional costs. This study aimed to determine the resistance of nine rice varieties commonly planted by farmers against the tungro. The study was conducted in a greenhouse, and the source of the tungro virus inoculum was taken from the Lintau area of ??West Sumatra Province. The results showed that of the nine varieties tested, IR 42 was resistant to the tungro attack, seven others were classified as moderate, and one variety was classified as vulnerable. The moderate resistant varieties were Anak Daro, Cisokan, Cantik Manih, Saganggam Panuah, Junjuang, Kuriek Kusuik, and Caredek Merah. In contrast, the variety that classified as vulnerable was Batang Sungkai due to the symptoms of attack more clearly than on other varieties, the fastest of a period of symptoms appear (10.15 days), the highest disease incidence (80.62%) with a disease index of 7.65.