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All Journal Bioeksperimen: Jurnal Penelitian Biologi NURSING UPDATE JURNAL ILMIAH ILMU KEPERAWATAN Journal of Tropical Chemistry Research and Education Journal Of Health Innovation And Community Services
Prasasti, Azmi
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Agriculture, Biological Sciences & Forestry Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Antibacterial Activity Test of Ethanol Extract of Robusta Coffee Beans (Coffea canephora) on Staphylococcus aureus and Escherichia coli bacteria Prasasti, Azmi; Prayudeni, Sari; Sundari, Fira
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 15 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v15i2.2168

Abstract

Robusta coffee is one of the most common types of coffee in Indonesia. Robusta coffee has secondary metabolites that function as antibacterial. The aim of this research was to determine the antibacterial activity of ethyl acetate extract of robusta coffee beans against Staphylococcus aureus and Escherichia coli bacteria. The method for in vitro antibacterial testing uses the disc diffusion test method. The extract concentrations used were 30%, 50%, 70% and 90%. The results showed that for S.aureus bacteria, the highest extract inhibition zone diameter was 22.1 mm at a concentration of 90%, and the lowest inhibitory zone diameter was 13.5 mm at a concentration of 30%. For E.coli bacteria, the diameter of the highest extract inhibitory value was 18.9 mm at a concentration of 90% and the diameter of the lowest extract inhibition zone was 9.4 mm at a concentration of 30%. The conclusion of this study shows that the diameter of the inhibition zone in S.aureus bacteria is greater than E.coli.
Uji Aktivitas Antibakteri Ekstrak Etanol Bunga Telang (Clitoria ternatea) pada bakteri Staphylococcus aureus dan Escherichia coli Prasasti, Azmi; Fitria Lestari, Ima; Anggraini, Anita
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 15 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v15i2.2196

Abstract

Butterfly pea flowers have benefits in almost all parts of the plant. One of the secondary metabolites in butterfly pea flowers functions as an antibacterial. This research aims to determine the ability of butterfly pea flower extract as an antibacterial against Staphylococcus aureus and Escherichia coli bacteria. The extraction method used was maceration with 70% ethanol solvent. The largest diameter of the inhibitory zone for S.aureus bacteria was found at a concentration of 90% butterfly pea flower extract of 20.8 mm. likewise for E.coli, the largest inhibitory zone diameter at a 90% concentration of butterfly pea flower extract was 15.4 mm
Standardization of Telang Flower Extract for Bioactive Compound Assessment using Solvent Variations Prasasti, Azmi; Prayudeni, Sari; Wijiani, Nina; Alfiansyah, M.Dzikry; Dwi Sasongko, Rio
Journal of Tropical Chemistry Research and Education Vol. 7 No. 1 (2025)
Publisher : Chemistry Education Department, Faculty of Tarbiyah and Education, UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jtcre.2025.71-05

Abstract

Telang flowers are found in many tropical areas, one of whuch is in Indonesia. The content of telang flowers can be used as anticancer, anti-inflammatory and antimicrobial. The amount of healt potential contained in telang flowers is important to do standardization. This study aims to determine the standardisation of telang flower extract in maceration method with 96% etanhol, ethyl acetate and n-hexane solvents. Standardisation testing includes specific and non-specific parametes. The results showed that the telang flowers used was included in the Clitoria ternatea species. Weight yield, drying shrinkage, specific gravity, moisture content and ash content have met the standard. The largest yield weight is found in ethanol solvent which is 11.83%. The highest secondary metabolite content was found in ethanol solvent, namely alkaloids, flavonoids, tanins, and saponin. While in n-hexane solvent there were only terpenoids and steroids.
Sosialisasi dan Pemberian Tablet Tambah Darah (TTD) Remaja Putri SMP Muhammadiyah 3 Banyuwangi Kustriyani, Anung; Deviani, Dita Amanda; Prasasti, Azmi
Journal of Health Innovation and Community Services Vol. 2 No. 1 (2023): Journal of Health Innovation and Community Services
Publisher : PPPM Stikes Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54832/jhics.v2i1.110

Abstract

Anemia is a condition that can cause disturbances in young women. Young women can experience anemia due to menstruation. To prevent anemia in young women is consumption of Blood Supplement Tablets. The Government's recommended Blood Supplement tablets contain 60 mg of iron and 0.4 mg of folic acid. Consumption of Iron Blood Tablets regularly once a week for 52 weeks. Purpose To provide knowledge and provision and assistance in taking Blood Supplement Tablets. Method Community service begins with conducting a preliminary survey to find out the problem. Video screenings, outreach, giving and assisting in drinking blood supplement tablets were also carried out. Results All students participated enthusiastically in the activity until the end. There were several questions asked to the speaker during the discussion. Blood Supplement Tablets can be taken by all female students. Conclusion Community service activities can provide knowledge and assistance in taking Blood Supplement Tablets to all female students.
Uji Aktivitas Antibakteri Daun Singkong (Manihot esculenta) Pada Bakteri Staphylococcus aureus dan Eschrichia coli Prasasti, Azmi; Kustriyani, Anung; Udianto, Abi Mas; Permatasari, Velisia Dini; Oktalia, Putri; Nursiyatin, Nursiyatin
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 1: March 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i1.16133

Abstract

Staphylococcus aureus and Escherichia coli are bacteria that cause infections and they are often found in the community. Infections caused by these bacteria are usually treated with antibiotics. However, excessive and uncontrolled use of antibiotics can lead to antibiotic resistance or immunity. This can result the infection will be more difficult to treat because of the bacteria's resistance to the antibiotic. Thus,  the treatment to minimize side effects of antibiotics resistance is needed. One of the traetment is using the herbal ingredients. Cassava leaves are plants with various properties, one of which is antibacterial. Therefore, this study aimed to determine the content of secondary metabolites and to test the antibacterial activity of cassava leaf extract on S. aureus and E. coli bacteria. The extraction method uses maceration. Antibacterial test using paper disc method test. Variations in the concentration of cassava leaf extract were 50,000 ppm, 100,000 ppm, and 150,000 ppm. The results showed that cassava leaf extract contained alkaloids, flavonoids, tannins, saponins and was able to inhibit the growth of S. aureus and E. coli bacteria. In S. aureus, the concentration of 150,000 ppm produced the highest inhibition zone of 11.1 mm and the concentration of 50,000 ppm produced the lowest inhibition zone of 8.6 mm. In E. coli a concentration of 150,000 ppm resulted in the highest inhibition zone of 5.2 mm and the lowest concentration of 3.5 mm. The response of cassava leaf extract was classified as a moderate response in inhibiting the growth of S. aureus bacteria. While producing a weak response in inhibiting the growth of E. coli bacteria. Statistical test for the inhibition zone of S. aureus showed normality of 0.500 and homogeneous 0.286, while for the inhibition zone of E.coli showed normal 0.96 and homogeneous 0.281.
Testing The Fe Content of Green Spinach Leaf Extract Using the AAS Spectrophotometry Method Prasasti, Azmi; Sari Prayudeni; Abi Mas Udianto; Stephanie Devi Artemesia; Anung Kustriyani; Elsa sarif
Journal of Tropical Chemistry Research and Education Vol. 8 No. 1 (2026)
Publisher : Chemistry Education Department, Faculty of Tarbiyah and Education, UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jtcre.2026.81-03

Abstract

Green spinach (Amaranthus hybridus L.) is a plant that contains vitamin K, vitamin C, vitamin E, vitamin A, and vitamin B complex. Green spinach is rich in iron to prevent anemia. Iron plays an important role in the manufacture of hemoglobin, a protein in blood cells, regular consumption of green spinach can increase hemoglobin levels. The purpose of this study was to determine the level of Fe in an extract of spinach leaves with the maceration method of aquadest solvent. Measurement of Fe levels using AAS Spectrophotometry (Atomic Absorption Spectrophotometry). The results of the study obtained the Fe content of thick spinach leaf extract of 0.0190%.
Co-Authors Abi Mas Udianto Alfiansyah, M.Dzikry Anita Anggraini Anung Kustriyani Deviani, Dita Amanda Dwi Sasongko, Rio Elsa sarif Fitria Lestari, Ima Kustriyani, Anung Nina Wijiani Nursiyatin, Nursiyatin Oktalia, Putri Permatasari, Velisia Dini Prayudeni, Sari Sari Prayudeni Stephanie Devi Artemesia Sundari, Fira Udianto, Abi Mas