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Antibacterial Activity Test of Ethanol Extract of Robusta Coffee Beans (Coffea canephora) on Staphylococcus aureus and Escherichia coli bacteria Prasasti, Azmi; Prayudeni, Sari; Sundari, Fira
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 15 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v15i2.2168

Abstract

Robusta coffee is one of the most common types of coffee in Indonesia. Robusta coffee has secondary metabolites that function as antibacterial. The aim of this research was to determine the antibacterial activity of ethyl acetate extract of robusta coffee beans against Staphylococcus aureus and Escherichia coli bacteria. The method for in vitro antibacterial testing uses the disc diffusion test method. The extract concentrations used were 30%, 50%, 70% and 90%. The results showed that for S.aureus bacteria, the highest extract inhibition zone diameter was 22.1 mm at a concentration of 90%, and the lowest inhibitory zone diameter was 13.5 mm at a concentration of 30%. For E.coli bacteria, the diameter of the highest extract inhibitory value was 18.9 mm at a concentration of 90% and the diameter of the lowest extract inhibition zone was 9.4 mm at a concentration of 30%. The conclusion of this study shows that the diameter of the inhibition zone in S.aureus bacteria is greater than E.coli.
Uji Aktivitas Antibakteri Ekstrak Etanol Bunga Telang (Clitoria ternatea) pada bakteri Staphylococcus aureus dan Escherichia coli Prasasti, Azmi; Fitria Lestari, Ima; Anggraini, Anita
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 15 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v15i2.2196

Abstract

Butterfly pea flowers have benefits in almost all parts of the plant. One of the secondary metabolites in butterfly pea flowers functions as an antibacterial. This research aims to determine the ability of butterfly pea flower extract as an antibacterial against Staphylococcus aureus and Escherichia coli bacteria. The extraction method used was maceration with 70% ethanol solvent. The largest diameter of the inhibitory zone for S.aureus bacteria was found at a concentration of 90% butterfly pea flower extract of 20.8 mm. likewise for E.coli, the largest inhibitory zone diameter at a 90% concentration of butterfly pea flower extract was 15.4 mm
Sosialisasi Tanaman Obat Keluarga Anti Hipertensi Di Desa Kemiren, Banyuwangi Prasasti, Azmi; Wijiyani, Nina; Ramadhani, Latansa Nova; Ummi, Hirsa Derratul
WASATHON Jurnal Pengabdian Masyarakat Vol 2 No 02 (2024)
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/wasathon.v2i02.1013

Abstract

Hipertensi is a non-communicable disease in individuals which is characterizes by an increase in blood pressure of more than 140/90 mmHg. Controlling blood pressure and preventing complications of hypertension is influenced by several factors, including the patient’s lack of knowledge about hypertension. Medicinal plants are an important part of self-medication that can be carried out by the community to prevent hypertension. Several TOGA’s are well known as medicines for hipertension, one of which is ginger dan startfruit. This cummunity service aims to increase knowledge about hypertension and its prevention through TOGA to PKK mothers in Kemiren Village, Banyuwangi. The method is carried out through conselling and measuring the level of knowledge of hypertnesion through pre-post test result which are tested statistically using the Shapiro Wilk test. The result of the pre-post test assessment showed that the mean knowledge related to hypertension among respondents, before counseling, was 18.60 with standard deviation of 11.35. Meanwhile, ater counseling it was 87.80 with a standard deviation of 9.86. In conclusion, there was an increase in knowledge after the counseling was 4.7 times better than before the counseling
The Penyuluhan Perilaku Hidup Bersih dan Sehat di SDN 02 Telemung, Banyuwangi Prasasti, Azmi; Prayudeni, Sari; Kustriyani, Anung; Deviani, Dita Amanda; Gymnastiar, Farhan; Maulidia, Maulidia
WASATHON Jurnal Pengabdian Masyarakat Vol 2 No 04 (2024)
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/wasathon.v2i04.1452

Abstract

The purpose of this community service is to improve the understanding and application of clean and healthy living behavior (PHBS) among students of SDN 02 Telemung, through socialization and education by lecturers and students of D3 Pharmacy STIKES Banyuwangi. The methods used in this service include initial observation, socialization and education, implementation and evaluation. Initial observations were made to determine the condition of students' understanding of PHBS. Socialization and education are carried out with the aim of providing clear and interesting information about the importance of personal hygiene, environmental sanitation, and healthy lifestyles. Implementation was carried out by direct application of clean and healthy living habits at school, followed by evaluation using pre and post tests to measure changes in student understanding. The results of the activity showed high enthusiasm from students in participating in socialization and education. The pre and post-test evaluation showed a significant increase in understanding, with an average pre-test score of 45 and post-test score of 85. This shows that this activity has succeeded in increasing students' awareness and knowledge of the importance of PHBS
Standardization of Telang Flower Extract for Bioactive Compound Assessment using Solvent Variations Prasasti, Azmi; Prayudeni, Sari; Wijiani, Nina; Alfiansyah, M.Dzikry; Dwi Sasongko, Rio
Journal of Tropical Chemistry Research and Education Vol. 7 No. 1 (2025)
Publisher : Chemistry Education Department, Faculty of Tarbiyah and Education, UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jtcre.2025.71-05

Abstract

Telang flowers are found in many tropical areas, one of whuch is in Indonesia. The content of telang flowers can be used as anticancer, anti-inflammatory and antimicrobial. The amount of healt potential contained in telang flowers is important to do standardization. This study aims to determine the standardisation of telang flower extract in maceration method with 96% etanhol, ethyl acetate and n-hexane solvents. Standardisation testing includes specific and non-specific parametes. The results showed that the telang flowers used was included in the Clitoria ternatea species. Weight yield, drying shrinkage, specific gravity, moisture content and ash content have met the standard. The largest yield weight is found in ethanol solvent which is 11.83%. The highest secondary metabolite content was found in ethanol solvent, namely alkaloids, flavonoids, tanins, and saponin. While in n-hexane solvent there were only terpenoids and steroids.
Sosialisasi dan Pemberian Tablet Tambah Darah (TTD) Remaja Putri SMP Muhammadiyah 3 Banyuwangi Kustriyani, Anung; Deviani, Dita Amanda; Prasasti, Azmi
Journal of Health Innovation and Community Services Vol. 2 No. 1 (2023): Journal of Health Innovation and Community Services
Publisher : PPPM Stikes Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54832/jhics.v2i1.110

Abstract

Anemia is a condition that can cause disturbances in young women. Young women can experience anemia due to menstruation. To prevent anemia in young women is consumption of Blood Supplement Tablets. The Government's recommended Blood Supplement tablets contain 60 mg of iron and 0.4 mg of folic acid. Consumption of Iron Blood Tablets regularly once a week for 52 weeks. Purpose To provide knowledge and provision and assistance in taking Blood Supplement Tablets. Method Community service begins with conducting a preliminary survey to find out the problem. Video screenings, outreach, giving and assisting in drinking blood supplement tablets were also carried out. Results All students participated enthusiastically in the activity until the end. There were several questions asked to the speaker during the discussion. Blood Supplement Tablets can be taken by all female students. Conclusion Community service activities can provide knowledge and assistance in taking Blood Supplement Tablets to all female students.
Uji Aktivitas Antibakteri Daun Singkong (Manihot esculenta) Pada Bakteri Staphylococcus aureus dan Eschrichia coli Prasasti, Azmi; Kustriyani, Anung; Udianto, Abi Mas; Permatasari, Velisia Dini; Oktalia, Putri; Nursiyatin, Nursiyatin
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 1: March 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i1.16133

Abstract

Staphylococcus aureus and Escherichia coli are bacteria that cause infections and they are often found in the community. Infections caused by these bacteria are usually treated with antibiotics. However, excessive and uncontrolled use of antibiotics can lead to antibiotic resistance or immunity. This can result the infection will be more difficult to treat because of the bacteria's resistance to the antibiotic. Thus,  the treatment to minimize side effects of antibiotics resistance is needed. One of the traetment is using the herbal ingredients. Cassava leaves are plants with various properties, one of which is antibacterial. Therefore, this study aimed to determine the content of secondary metabolites and to test the antibacterial activity of cassava leaf extract on S. aureus and E. coli bacteria. The extraction method uses maceration. Antibacterial test using paper disc method test. Variations in the concentration of cassava leaf extract were 50,000 ppm, 100,000 ppm, and 150,000 ppm. The results showed that cassava leaf extract contained alkaloids, flavonoids, tannins, saponins and was able to inhibit the growth of S. aureus and E. coli bacteria. In S. aureus, the concentration of 150,000 ppm produced the highest inhibition zone of 11.1 mm and the concentration of 50,000 ppm produced the lowest inhibition zone of 8.6 mm. In E. coli a concentration of 150,000 ppm resulted in the highest inhibition zone of 5.2 mm and the lowest concentration of 3.5 mm. The response of cassava leaf extract was classified as a moderate response in inhibiting the growth of S. aureus bacteria. While producing a weak response in inhibiting the growth of E. coli bacteria. Statistical test for the inhibition zone of S. aureus showed normality of 0.500 and homogeneous 0.286, while for the inhibition zone of E.coli showed normal 0.96 and homogeneous 0.281.
Analisis Kadar Kafein pada Kopi Liberika (Coffea liberica) Menggunakan Metode Spektrofotometri UV-Vis Prasasti, Azmi; Kustriyani, Anung; Udianto, Abi Mas; Delafani, Ulil
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 1 (2026): January (In Progress)
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i1.924

Abstract

Coffee Liberica (Coffea liberica) is one of the coffee varieties that has the potential to be developed, but information on the effect of roasting levels on caffeine content is limited. Meanwhile, caffeine levels are an important parameter that affects quality and sensory characteristics. This study aims to qualitatively identify the presence of caffeine and quantitatively determine caffeine levels in liberica coffee with different roast levels, namely light, medium, and dark roast. The research was conducted experimentally in the laboratory using the Qualitative Test of the Parry method and the quantitative test of the UV-Vis Spectrophotometry method. The sample was in the form of liberica coffee beans at three roasting levels and was repeated three times. The results of the qualitative test showed that all samples of liberica coffee at the three levels of positive roasting contained caffeine which was characterized by the formation of a green color in the Parry test. The results of the quantitative analysis showed that the highest caffeine level was found at the light roast level of 116.412 mg/g, followed by medium at 102.381 mg/g, and the lowest at dark roast at 91.036 mg/g. The decrease in caffeine content as the temperature increases and the length of the roasting time shows that the roasting process has a significant effect on the stability of caffeine. The results of this study are expected to make a scientific contribution to the development of the chemical study of Liberica coffee, as well as become a practical reference for coffee industry players in determining the roasting level according to the characteristics of caffeine.