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Antibacterial Activity Test of Ethanol Extract of Robusta Coffee Beans (Coffea canephora) on Staphylococcus aureus and Escherichia coli bacteria Prasasti, Azmi; Prayudeni, Sari; Sundari, Fira
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 15 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v15i2.2168

Abstract

Robusta coffee is one of the most common types of coffee in Indonesia. Robusta coffee has secondary metabolites that function as antibacterial. The aim of this research was to determine the antibacterial activity of ethyl acetate extract of robusta coffee beans against Staphylococcus aureus and Escherichia coli bacteria. The method for in vitro antibacterial testing uses the disc diffusion test method. The extract concentrations used were 30%, 50%, 70% and 90%. The results showed that for S.aureus bacteria, the highest extract inhibition zone diameter was 22.1 mm at a concentration of 90%, and the lowest inhibitory zone diameter was 13.5 mm at a concentration of 30%. For E.coli bacteria, the diameter of the highest extract inhibitory value was 18.9 mm at a concentration of 90% and the diameter of the lowest extract inhibition zone was 9.4 mm at a concentration of 30%. The conclusion of this study shows that the diameter of the inhibition zone in S.aureus bacteria is greater than E.coli.
The Penyuluhan Perilaku Hidup Bersih dan Sehat di SDN 02 Telemung, Banyuwangi Prasasti, Azmi; Prayudeni, Sari; Kustriyani, Anung; Deviani, Dita Amanda; Gymnastiar, Farhan; Maulidia, Maulidia
WASATHON Jurnal Pengabdian Masyarakat Vol 2 No 04 (2024)
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/wasathon.v2i04.1452

Abstract

The purpose of this community service is to improve the understanding and application of clean and healthy living behavior (PHBS) among students of SDN 02 Telemung, through socialization and education by lecturers and students of D3 Pharmacy STIKES Banyuwangi. The methods used in this service include initial observation, socialization and education, implementation and evaluation. Initial observations were made to determine the condition of students' understanding of PHBS. Socialization and education are carried out with the aim of providing clear and interesting information about the importance of personal hygiene, environmental sanitation, and healthy lifestyles. Implementation was carried out by direct application of clean and healthy living habits at school, followed by evaluation using pre and post tests to measure changes in student understanding. The results of the activity showed high enthusiasm from students in participating in socialization and education. The pre and post-test evaluation showed a significant increase in understanding, with an average pre-test score of 45 and post-test score of 85. This shows that this activity has succeeded in increasing students' awareness and knowledge of the importance of PHBS
Standardization of Telang Flower Extract for Bioactive Compound Assessment using Solvent Variations Prasasti, Azmi; Prayudeni, Sari; Wijiani, Nina; Alfiansyah, M.Dzikry; Dwi Sasongko, Rio
Journal of Tropical Chemistry Research and Education Vol. 7 No. 1 (2025)
Publisher : Chemistry Education Department, Faculty of Tarbiyah and Education, UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jtcre.2025.71-05

Abstract

Telang flowers are found in many tropical areas, one of whuch is in Indonesia. The content of telang flowers can be used as anticancer, anti-inflammatory and antimicrobial. The amount of healt potential contained in telang flowers is important to do standardization. This study aims to determine the standardisation of telang flower extract in maceration method with 96% etanhol, ethyl acetate and n-hexane solvents. Standardisation testing includes specific and non-specific parametes. The results showed that the telang flowers used was included in the Clitoria ternatea species. Weight yield, drying shrinkage, specific gravity, moisture content and ash content have met the standard. The largest yield weight is found in ethanol solvent which is 11.83%. The highest secondary metabolite content was found in ethanol solvent, namely alkaloids, flavonoids, tanins, and saponin. While in n-hexane solvent there were only terpenoids and steroids.