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Rori, Chindy Achika
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Other

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Aktivitas Enzim Ekstraseluler dari Bakteri Endofit Tumbuhan Mangrove Avicennia marina Rori, Chindy Achika; Kandou, Febby Ester Fany; Tangapo, Agustina Monalisa
JURNAL BIOS LOGOS Vol 10, No 2 (2020): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.2.2020.28338

Abstract

ABSTRAKBakteri endofit merupakan salah satu sumber penghasil senyawa ekstraseluler yaitu enzim. Enzim dari bakteri endofit lebih menguntungkan dan produksinya lebih cepat. Penelitian ini bertujuan untuk menganalisis kemampuan aktivitas enzim ekstraseluler dari bakteri endofit tumbuhan mangrove Avicennia marina. Metode penelitian yang digunakan yaitu eksploratif eksperimental, melakukan isolasi bakteri dari tumbuhan mangrove A. marina dan selanjutnya dilakukan uji potensi aktivitas enzim ekstraseluler dari isolat bakteri endofit. Hasil isolasi memperoleh tujuh isolat bakteri endofit dari tumbuhan A. marina, isolat endofit tersebut mampu menghasilkan aktivitas enzim ekstraseluler yaitu empat isolat menghasilkan enzim amilase, enam isolat menghasilkan protease, satu isolat menghasilkan selulase dan dua isolat menghasilkan gelatinase.Kata kunci: bakteri endofit; Avicennia marina; amilase; protease; selulase; gelatinase ABSTRACTEndophytic bacteria are one source that can produce extracellular compounds, namely enzymes. Enzymes from endophytic bacteria are more profitable and can produce faster. This study aims to analyze the ability of extracellular enzyme activity from endophytic bacteria in mangrove plants Avicennia marina. This research used experimental explorative method, isolating bacteria from mangrove plant A. marina and then testing the potential of enzyme extracellular activity from endophytic bacteria isolated. Isolating result obtained seven endophytic bacterial isolates from A. marina plants,this endophytic isolates are able to produce extracellular enzyme activity is four isolates can produced amylase enzyme, six isolated can produced protease, one isolated can produced cellulase and two isolated can produce gelatinase. Keywords: Endophytic bacteria; Avicennia marina; amylase, protease, cellulase, gelatinase
Isolation and Antibacterial Test of Endophytic Bacteria of Avicennia marina Mangroves Rori, Chindy Achika; Kandou, Febby Ester Fany; Tangapo, Agustina Monalisa
Koli Journal : English Language Education Vol 1 No 1 (2020): Koli Journal: English Language Education
Publisher : Prodi Pendidikan Bahasa Inggris, Program Studi Diluar Kampus Utama (PSDKU) Unpatti-MBD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/koli.1.1.1-7

Abstract

Endophytic bacteria are a source of extracellular compounds such as antimicrobial compounds. Endophytic bacteria and host plants have a symbiotic relationship of mutualism that allows bacteria to produce the same bioactive compounds as those contained in the host plant. This study aims to isolate and test the antibacterial potential of endophytic bacterial Avicennia marina mangroves. The research method used is experimental explorative, isolating bacteria from A. marina mangrove plants and then testing the potential of extracellular enzyme activity from endophytic bacterial isolates. The results of isolation obtained seven isolates of endophytic bacteria from plants A. Marina, the endophytic isolates were able to produce extracellular enzyme activity, namely two isolates had the ability to be antibacterial against Escherichia coli test bacteria.
Co-Authors Febby Ester Fany Kandou Tangapo, Agustina Monalisa