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Kamto, Yoel William Prasetyo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Development of Commercial Sterility Detection Method Based on Chromogenic Media Agar in UHT Coconut Cream Liquid Products Tangkau, Ester Ruth Youla; Rori, Chindy Achika; Kamto, Yoel William Prasetyo; Mangangantung, Brayen; Tangapo, Agustina Monalisa
JURNAL BIOS LOGOS Vol. 15 No. 3 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i3.64802

Abstract

Commercial sterility analysis is a crucial step in quality control of Ultra High Temperature (UHT) products such as coconut cream liquid products produced by PT Sasa Inti Minahasa Selatan, to ensure the safety and stability of shelf life. Stability incubation testing takes a long time, which is 7-14 days. Development of commercial sterility methods using Chromogenic Agar selective media is needed to cut the incubation time that is too long. Agar Chromogenic Media is tested for its ability to detect common contaminants in UHT coconut cream products, in particular thermophilic and mesophilic microorganisms that cause spoilage, with a short incubation time. UHT coconut cream samples are prepared by artificial and natural inoculation. Validation was carried out by comparing the results on standard media based on sensitivity, specificity and Time-to-result (TTR) parameters. The results showed a real difference between NA/TGEA media and chromogenic media so that the reading of bacterial colonies growing on chromogenic media was clearer and easier to read due to the change in color and short incubation time where the bacteria causing decay had grown on day 5 with a reading of results that took only 24 hours. Based on the validation that has been carried out, chromogenic selective media can accelerate product release time and improve quality control efficiency in the UHT coconut cream industry.
Keanekaragaman Jenis Makroalga Di Perairan Kecamatan Tombariri Kawasan Taman Nasional Bunaken Sulawesi Utara Kamto, Yoel William Prasetyo; Dapas, Farha; Rondonuwu, Sendy
JURNAL BIOS LOGOS Vol. 14 No. 1 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i1.55022

Abstract

Makroalga menjadi salah satu organisme laut yang melimpah di perairan. Fungsi makroalga di alam yaitu menjadi sumber makanan serta dapat menyediakan habitat untuk biota laut. Makroalga juga banyak memberikan manfaat dalam bidang pangan, ekonomi dan kesehatan untuk itu perlu dilakukannya penelitian terhadap makroalga. Tujuan penelitian ini untuk menganalisis keanekaragaman jenis makroalga di Perairan Kecamatan Tombariri Sulawesi Utara. Penelitian ini menggunakan metode purposive sampling dengan pembuatan garis transek dan plot pada zona littoral. Inventarisasi makroalga yang ditemukan di Perairan Kecamatan Tombariri terdiri dari 17 famili, 32 jenis dan 3,705 individu makroalga yang terbagi dalam tiga divisi. Terdapat 20 jenis makroalga Divisi Chlorophyta, 4 jenis makroalga Ochrophyta dan 8 jenis makroalga Rhodophyta. Indeks keanekaragaman makroalga di Perairan Kecamatan Tombariri termasuk dalam kategori sedang dengan nilai (2,79). Indeks keanekaragaman stasiun I Desa Poopoh memiliki nilai (2,78) dan stasiun II Desa Teling (2,44). Kondisi jenis makroalga cukup stabil dan tekanan ekologis sedang.
Co-Authors Brayen Mangangantung Farha Dapas, Farha Rori, Chindy Achika Sendy Rondonuwu, Sendy Tangapo, Agustina Monalisa Tangkau, Ester Ruth Youla