Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal Indonesian Journal of Tropical and Infectious Disease Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Sulaeman, Rizal Pratama
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Medicine & Pharmacology Public Health

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Evaluation of Epidemiological Investigation 1 -2-5 Implementation Program in Sukabumi Prasetyowati, Heni; Widawati, Mutiara; Fuadzy, Hubullah; Fuadiyah, M. Ezza Azmi; Ginanjar, Aryo; Nurindra, Rohmansyah W; Ridwan, Wawan; Hodijah, Dewi Nur; Sulaeman, Rizal Pratama
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 3 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i3.29599

Abstract

Epidemiological investigations as part of the malaria surveillance system in Indonesia are carried out through the 1-2-5 method. Assessing the 1-2-5 strategy compliance level at the district level is the first step towards determining whether the surveillance and response strategy is working as planned or not. This study was conducted in order to determine whether PE 1-2-5 had been implemented in health centers (puskesmas) in malaria receptive areas according to the technical guidelines issued by the Indonesian Ministry of Health. Health centers were determined through purposive sampling technique. The sampling is determined by selecting health centers that have been doing malaria vector control service in 2018 and 2019. Ten Puskesmas in malaria receptive areas in Sukabumi District were selected. The informants in this study were the key players in the malaria program at the health centers: the head of the health centers, the manager of the malaria program, and the village malaria officer (JMD) who were involved in the vector control process in 2018 and 2019 at the selected health centers. Data collection was conducted through in-depth interviews done by researcher with informants. The interview showed that the malaria program personnel in Sukabumi are doing the strategy as best as possible in order to achieve malaria elimination. 1-2-5 surveillance program in Sukabumi district has been implemented even though the implementation is not as ideal as the technical guidelines suggested by the Indonesian Ministry of Health, Sukabumi district still applied the strategy based on it by adjusting various aspects (resource situation and the availability of facilities) to the suitable condition in Sukabumi.
OPTIMIZATION AND EFFICIENCY ANALYSIS OF REAL-TIME PCR FOR LEPTOSPIRA SPP. DIAGNOSIS BASED ON THE LIPL32 GENE Sulaeman, Rizal Pratama; Rohayati; Merdekawati, Fusvita; Sundara Mulia, Yuliansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10646

Abstract

Leptospirosis is a widespread zoonotic infection that endangers the health of both humans and animals, particularly in tropical and subtropical regions. Therefore, timely, sensitive and specific laboratory confirmation is essential for early clinical management. The LipL32 gene is a highly conserved virulence factor in pathogenic Leptospira. Real-time PCR provides rapid detection with high sensitivity and specificity. This study optimized real-time PCR conditions by evaluating annealing temperatures (60°C, 61°C, 62°C), primer concentrations (500 nM, 700 nM, 900 nM), and probe concentrations (250 nM, 300 nM). PCR efficiency was analyzed using absolute quantification with serial DNA dilutions (100 to 10⁻⁴). The optimal conditions were 60°C annealing temperature, 500 nM primers, and 300 nM probes. Near-ideal efficiency (97%) was achieved at 60°C with 500 nM primers and 250 nM or 300 nM probes, while 103% efficiency was observed at 61°C with 500 nM primers and 250 nM probes. This optimization enhances Leptospira detection accuracy using real-time PCR.
OPTIMIZATION AND EFFICIENCY ANALYSIS OF REAL-TIME PCR FOR LEPTOSPIRA SPP. DIAGNOSIS BASED ON THE LIPL32 GENE Sulaeman, Rizal Pratama; Rohayati; Merdekawati, Fusvita; Sundara Mulia, Yuliansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10646

Abstract

Leptospirosis is a widespread zoonotic infection that endangers the health of both humans and animals, particularly in tropical and subtropical regions. Therefore, timely, sensitive and specific laboratory confirmation is essential for early clinical management. The LipL32 gene is a highly conserved virulence factor in pathogenic Leptospira. Real-time PCR provides rapid detection with high sensitivity and specificity. This study optimized real-time PCR conditions by evaluating annealing temperatures (60°C, 61°C, 62°C), primer concentrations (500 nM, 700 nM, 900 nM), and probe concentrations (250 nM, 300 nM). PCR efficiency was analyzed using absolute quantification with serial DNA dilutions (100 to 10⁻⁴). The optimal conditions were 60°C annealing temperature, 500 nM primers, and 300 nM probes. Near-ideal efficiency (97%) was achieved at 60°C with 500 nM primers and 250 nM or 300 nM probes, while 103% efficiency was observed at 61°C with 500 nM primers and 250 nM probes. This optimization enhances Leptospira detection accuracy using real-time PCR.
Co-Authors Dewi Nur Hodijah Ginanjar, Aryo Heni Prasetyowati Hubullah Fuadzy M. Ezza Azmi Fuadiyah Merdekawati, Fusvita Mutiara Widawati Nurindra, Rohmansyah W Rohayati Sundara Mulia, Yuliansyah Wawan Ridwan