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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Jurnal Atma Inovasia Jurnal Kefarmasian Indonesia
Widhiastuti, Stefani Santi
Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Other

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Journal : BERITA BIOLOGI

 1 
PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2 Stefani Santi Widhiastuti; Bernadia Branitamahisi; Nor Sri Inayati; Ida Ayu Preharsini; Demas Bayu Handika; Ahmad Hamim Sadewa; Abdurahman Laqif; Sofia Mubarika Haryana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3830

Abstract

Diabetes mellitus type 2 is the most common type of diabetes. This study was conducted to determine the effect of Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) in Homeostatic Model Assessment of β-cell function (HOMA-β) value, normal Langerhans cells, and Transcription Factor 7-Like 2 (TCF7L2) gene expression in type 2 diabetic rats model. As many as 27 male Sprague Dawley rats were divided into 3 study research groups: normal control (9 normal rats), diabetic control (9 type 2 diabetic rats, induced by 60 mg/kg BW Streptozotocin and 120 mg/kg BW Nicotinamide i.p.), and treatment (9 type 2 diabetic rats treatment with 0.1 ml/200g BW MSC-CM i.p.). On day 30 after therapy, the expression of TCF7L2 gene was performed with real time-quantitative PCR (RT-qPCR). The HOMA-β value were calculated based on Fasting Insulins (FINs) levels and Fasting Blood Glucose (FBG) levels data from other research team members. Based on results, MSC-CM increases the HOMA-β value and amount of normal Langerhans cells of treatment group that indicates amelioration effect of MSC-CM, but there was no significant difference in TCF7L2 gene expression level between diabetic control and treatment group.  
FORMULASI LOTION EKSTRAK METHANOL DAUN KELOR (Moringa oleifera) DENGAN AKTIVITAS ANTIBAKTERI (Lotion Formulation of Moringa Leaves (Moringa Oleifera) Methanol Extract with Antibacterial Activity) Stefani Santi Widhiastuti
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i1.4293

Abstract

ABSTRACTThe skin has an important function in the body's first line of defense. Lotion is used to maintain skin health, one of which is from microorganism infection by the presence of antibacterial substances in the formula. One of the plants that has antibacterial activity is Moringa (Moringa oleifera). The aim of this study was to make a lotion formula containing methanol extract of Moringa leaves which has antibacterial activity. The research was carried out from the extraction stage, phytochemical assay, lotion formulation, and antibacterial activity assay of the extract and lotion. Based on the results of phytochemical assay, methanol extract of Moringa leaves contains saponins, flavonoids, tannins, alkaloids and steroids, but does not contain triterpenoids. Moringa leaves extract has antibacterial activity against E. coli, S. aureus, and P. aeruginosa, where the activity increases along with increasing extract concentration. The lotion formula with 3% extract content is the optimum formula with the best antibacterial activity compared to other formulas, lotion base, and positive control with very strong antibacterial activity, seen from the inhibition zone diameter of more than 20 mm against E. coli, S. aureus, and P. aeruginosa. Keywords: kelor (Moringa oleifera), antibacterial activity, lotion ABSTRAK Kulit memiliki fungsi penting dalam pertahanan pertama tubuh. Lotion digunakan untuk menjaga kesehatan kulit salah satunya dari infeksi mikroorganisme dengan adanya kandungan zat antibakteri dalam sediaan. Salah satu tanaman yang memiliki aktivitas antibakteri adalah kelor (Moringa oleifera). Tujuan penelitian ini adalah membuat formula lotion dengan kandungan ekstrak methanol daun kelor yang memiliki aktivitas antibakteri. Penelitian dilakukan mulai dari tahap ekstraksi, uji kandungan fitokimia ekstrak, uji aktivitas antibakteri ekstrak, pembuatan formula lotion dan uji aktivitas antibakteri lotion. Berdasarkan hasil uji fitokimia, ekstrak methanol daun kelor mengandung saponin, flavonoid, tanin, alkaloid dan steroid, namun tidak mengandung triterpenoid. Ekstrak methanol daun kelor memiliki aktivitas antibakteri terhadap E. coli, S. aureus, dan P. aeruginosa, dimana aktivitasnya semakin meningkat seiring dengan meningkatnya konsentrasi ekstrak. Formula lootion dengan kandungan ekstrak sebesar 3% merupakan formula optimum dengan aktivitas antibakteri paling baik dibandingkan formula lain, basis lotion, maupun kontrol positif dengan aktivitas antibakteri sangat kuat, dilihat dari diameter zona hambat lebih dari 20 mm terhadap bakteri E. coli, S. aureus, and P. aeruginosa. Kata kunci: kelor (Moringa oleifera), aktivitas antibakteri, lotion
Co-Authors Abdurahman Laqif Abdurahman Laqif Ahmad Hamim Sadewa Alvina, Devi Arsiningtyas, Ines Septi Atmodjo, Patricius Kianto Bernadia Branitamahisi Bernadia Branitamahisi Bernardus Boy Rahardjo Sidharta Boy Rahardjo Sidharta Demas Bayu Handika Dewi Retnaningati Exsyupransia Mursyanti Exsyupransia Mursyanti, Exsyupransia Gunawan, Yohanes Candra Hida, Fitriana Nur Laissya Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ines Septi Arsiningtyas Jovitta Theachrysilla Wiadji Mahardhika, I Wayan Swarautama Maria Ariska Happy Suntadi Meisy Michele Liony Maria Onibala Nor Sri Inayati Nor Sri Inayati Oktaviani, Yustina Wanda Dwi Onibala, Michele Liony Maria P Kianto Atmodjo Pantalea Edeilweiss Vitara Paramitha, Prizka Kezia Patricius Kianto Atmodjo Sendy Junedi Setiawan, Pamela Felita Sidharta, Bernardus Boy Rahardjo Sofia Mubarika Haryana Subandriyo, Jessica Rieko Suntadi, Maria Ariska Happy To'bungan, Nelsiani Wiadji, Jovitta Theachrysilla Yohanes Candra Gunawan Yustina Wanda Dwi Oktaviani