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All Journal Jurnal Medik Veteriner journal of Basic Medical Veterinary
Ernawati, Rahaju
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya Kirana, Maha; Ernawati, Rahaju; Rahmahani, Jola; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.103-108

Abstract

This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.
Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nurrahmad, Nanda Rino; Yuliani, M. Gandul Atik; Ernawati, Rahaju; Chusniati, Sri; Herupradoto, Eduardus Bimo Aksono; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.
Co-Authors Chusniati, Sri Fedik Abdul Rantam Gandul Atik Yuliani Herupradoto, Eduardus Bimo Aksono Kirana, Maha Nurrahmad, Nanda Rino Rahmahani, Jola Wahyuni, Retno Sri