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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Teknologi Laboratorium Jurnal Medik Veteriner Oto Rhino Laryngologica Indonesiana Journal Orthopaedi and Traumatology Surabaya (JOINTS) Journal of Parasite Science Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Current Biomedicine

Fedik Abdul Rantam

Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 2480199

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Immunobioinformatics analysis and phylogenetic tree construction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Indonesia: spike glycoprotein gene Ansori, Arif Nur Muhammad; Kharisma, Viol Dhea; Antonius, Yulanda; Tacharina, Martia Rani; Rantam, Fedik Abdul
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has spread worldwide and as a result, the World Health Organization (WHO) declared it a pandemic. At present, there are no approved vaccines against SARS-CoV-2. Therefore, the aim of this study was to predict epitope-based vaccines using bioinformatics approaches and phylogenetic tree construction of SARS-CoV-2 against the backdrop of the COVID-19 pandemic. In this study, we employed 27 isolates of SARS-CoV-2 spike glycoprotein genes retrieved from GenBankÂ® (National Center for Biotechnology Information, USA) and the GISAID EpiCoVâ„¢ Database (Germany). We analyzed the candidate epitopes using the Immune Epitope Database and Analysis Resource. Furthermore, we performed a protective antigen prediction with VaxiJen 2.0. Data for B-cell epitope prediction, protective antigen prediction, and the underlying phylogenetic tree of SARS-CoV-2 were obtained in this research. Therefore, these data could be used to design an epitope-based vaccine against SARS-CoV-2. However, the advanced study is recommended for confirmation (in vitro and in vivo).

Role of Preconditioning Adipose Mesenchymal Stem Cells in Regeneration Disk: a Review Article Romaniyanto; Hari Basuki Notobroto; Cita Rosita Sigit Prakoeswa; Fedik Abdul Rantam; Dwikora Novembri Utomo; Heri Suroto; Ferdiansyah
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14892

Intervertebral disk (IVD) degeneration (IDD) is a degenerative disease and often found in daily practice.The managements that were used so far are only symptomatic and have not been able to solve the diseasecompletely. The use of mesenchymal stem cells (MSCs) has been widely tested as a solution for theregeneration of IVD in IDD. Adipose-derived mesenchymal stem cells (ASCs) are one of the sources ofMSCs which were widely studied as an alternative. One of the factors that can influence the efficiency andcapacity of ASCs in IDD cases is preconditioning. Generally, there were three pre-conditioning strategies ofASCs before being used as therapy, (1) modifying the environment, (2) using Growth Factors, Cytokines,Chemokines, Hormones, (3) using pharmacological and chemical agents. Preconditioning on ASCs canchange its function in various ways. We tried to link all these things together so that we could know the roleof pre-conditioning ASCs in the regeneration disk.

Determinan Antigen Gen omp2a Brucella abortus Isolat Lokal Ratih Ratnasari; Didik Handijatno; . Suwarno; Fedik Abdul Rantam
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Penyakit Brucellosis pada sapi disebabkan oleh Brucella abortus dan dikenal sebagai penyakitreproduksi menular pada ternak. Brucellosis merupakan penyakit zoonosis karena dapat menularpada manusia. Penelitian ini bertujuan untuk mengetahui determinan antigen yang terdapat pada genomp2a B. abortus isolat lokal yang telah diblasting ke asam amino (protein). Sampel bakteri berasaldari sapi penderita Brucellosis asal Sulawesi Selatan dan Nusa Tenggara Timur. Gen omp2a diamplifikasimelalui tehnik Polymerase Chain Reaction (PCR) dengan menggunakan primer 2ab5F dan 2a900R.Produk PCR disekuensing untuk mendapatkan sekuen nukleotida gen omp2a B. abortus isolat lokal.Sekuen nukleotida ini dianalisis tingkat homologinya terhadap isolat asal mancanegara yang diaksesdari GenBank dengan menggunakan BLAST. Sekuen nukleotida gen omp2a diblasting ke asam aminokemudian dengan metode Kolaskar dan Tongaonkar antigenicity dapat diperoleh determinan antigenpada antigen protein membran luar (OMP) B. abortus isolat lokal. Hasil menunjukkan bahwa tingkathomologi antara sekuen gen omp2a B. abortus isolat lokal dengan isolat asal mancanegara mempunyaitingkat homologi yang tinggi (99% - 100%). Hasil prediksi determinan antigen didapatkan enamdeterminan antigen pada antigen OMP B. abortus isolat lokal.

Utilization of viable bone marrow derivat stem cells through an adaption in low oxigen tension as an attempt to increase cellular transplantation efficacy for spermatogenesis process Erma Safitri; Suzanita Utama; Candra Bumi; Sri Wigati Mardi; . Mulyani; . Helen; . Purwati; R. Heru Prasetyo; Mas’ud Hariadi; Fedik Abdul Rantam
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.411 KB)

Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.

The potential of mesenchymal stem‐cell secretome for regeneration of intervertebral disc: A review article Romaniyanto Romaniyanto; Cita Rosita Sigit Prakoeswa; Damayanti Tinduh; Hari Basuki Notobroto; Fedik Abdul Rantam; Dwikora Novembri Utomo; Heri Suroto; Ferdiansyah Ferdiansyah
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.63318

Low back pain is a crucial public health problem that is commonly associated with intervertebral disc de‐ generation and has vast socio‐economic impact worldwide. Current treatments for disc degeneration are conservative, non‐surgical, or surgical interventions, and there is no current clinical therapy aimed at directly reversing the degeneration. Given the limited capacity of intervertebral disc (IVD) cells to self‐repair, treatment aiming to regenerate IVDs is a topic of interest and mesenchymal stem cells (MSCs) have been identified as having potential in this regeneration. Recent studies have revealed that the benefits of MSC therapy could result from the molecules the cells secrete and that play principal roles in regulating essential biologic processes, rather than from the implanted cells themselves. Therefore, the objective of this study is to review the potential use of the MSC secretome to regenerate IVDs. Current evidence shows that the secretome may regenerate IVDs by modulating the gene expressions of nucleus pulposus cells (upregulation of keratin 19 and downregulation of matrix metalloproteinase 12 and matrix Gla protein) and stimulating IVD progenitor cells to repair the degenerated disc.

Antibody of goat zona pellucida-3 (gzp3) protein of mice(Mus musculus) block in vitro fertilization of mice as an animal model= Antibodi protein zona pelusida-3 kambing (gZP3) asal mencit(Mus musculus) mencegah ... Imam Mustofa; Laba Mahaputra; Yoes Priyatna Dachlan; Fedik Abdul Rantam; Suwarno .; Widjiati .; Aucky Hinting
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p

Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.954 KB)

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.

Penentuan Protein Antigen Limfosit Sapi yang Berasal dari Sapi Bali dan Sapi Peranakan Ongole (DETERMINATION OF THE PROTEIN OF BOVINE LYMPHOCYTE ANTIGEN, OF BALI CATTLE AND ONGOLE CROSS BREED CATTLE) Ni Ketut Suwiti; Fedik Abdul Rantam
Jurnal Veteriner Vol 4 No 2 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura Ni Ketut Suwiti; Fedik Abdul Rantam; I Nengah Kerta Besung
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.584 KB)

A study to detect the presence of bovine major histocompatibility complex (Bo-MHC) in maduracattle has been carried out. Lymphocyte samples were collected from 25 cattle. The protein of lymphocytesamples was analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) andtransferred onto nitrocellulose membrane. Bo-MHC proteins on the membrane were detected by monoclonalantibodies (anti-Bo-MHC class I) and BAQ150A (anti-Bo-MHC class II). The proteins of 48 kDa and 11kDa were detected by MAb B5C which appeared to be the alpha and beta chain of Bo-MHC class I. MAbBAQ150A detected protein band of 26 KDa which was likely to be the beta chain of Bo-MHC class II. Bo-MHC class I dan class II were clearly detected in madura cattle by MAbs which provides a basis for furtherinvestigation on the role of the MHC in the susceptibility the animals to many diseases.

Pemberian Buah Merah (Pandanus conoideus Lam) sebelum Dipapar Timah Hitam Menekan Ekspresi Caspase-8 dan Jumlah Sel Hofbauer Mencit (Mus musculus) Bunting (THE PROVISION OF RED FRUIT (Pandanus conoideus Lam) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Ika Wahyuni; Widjiati Widjiati; Sri Pantja Madyawati; Fedik Abdul Rantam
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.

Co-Authors . Helen . Mulyani . Purwati . PURWATI . Suwarno A'la, Rofiqul Abdurachman Abdurachman Ahmad Chusnu Romdhoni Amaq Fadholly Ami Ashariati Amrullah, Amirul Muslim Andi Yasmin Wijaya Andriyanto Andriyanto Anindya, Afifatus Annise Proboningrat Ardhanella, Sellianova Arif Nur Muhammad Ansori Aris Widayati Aswin, Ahmad Aucky Hinting Aulia Andi Mustika Azahro, Aisyah Bambang Sektiari Lukiswanto Boedi Setiawan Candra Bumi Cita Rosita Sigit Prakoeswa Damayanti Tinduh Desi Sandra Sari Diah Nugrahani Pristihadi Didik Handijatno Diyantoro Diyantoro, Diyantoro Dwikora Novembri Utomo Eduardus Bimo Aksono Herupradoto Emmanuel Djoko Poetranto ENDANG RETNOWATI KUSUMOWIDAGDO Endang Suprihati ERMA SAFITRI Ernawati, Rahaju Ernawati, Rahayu Ernie Maduratna Estoepangestie, Agnes Theresia Soelih Ferdiansyah Ferdiansyah Mahyudin Gilang Maulana Putra HAK HOTTA Hani Plumeriastuti Hari Basuki Notobroto Harijani, Nenny Helen Susilowati, Helen Henry Dominica Heri Suroto HERNOMO ONTOSENO KUSUMOBROTO I'tishom, Reny Ika WAHYUNI IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ira Sari Yudaniayanti Iwan Sahrial Hamid Jola Rahmahani Juniastuti Juniastuti Kadek Rachmawati Khairullah, Aswin Rafif Kharisma, Viol Dhea Kirana, Maha Kuncorojakti, Suryo Kusnoto Kusnoto, Kusnoto Laba Mahaputra Lina Noviyanti Sutardi Lucia Tri Suwanti, Lucia Tri Lukman Hakim Machin, Abdulloh MARIA INGE LUSIDA Mas’ud Hariadi Mouli Edward Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nasronudin Nasronudin Ni Ketut Suwiti Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Purwati Purwati Putih, Nurvita R. Heru Prasetyo Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Ratna Damayanti Retno Pudji Rahayu Ria Hanistya Rina Mutya Suzliana Romaniyanto Romaniyanto Romaniyanto Romy Muhammad Dary Mufa S.Pd. M Kes I Ketut Sudiana . Santoso, Kuncoro Puguh SOETJIPTO . Sri Hernawati Sri Pantja Madyawati Sri Wigati Mardi Steven Sheng Looi Suwarno . Suwarno Suwarno Suwarno Suwarno Suzanita Utama Tacharina, Martia Rani TAKAKO UTSUMI Tristiana Erawati Tyasningsih, Wiwiek Venri Novryantoro Wibi Riawan Widiyatno, Thomas Valentinus Widji Soeratri Widjiati . Widjiati Widjiati Widodo Ario Kentjono Wijaya, Andi Yasmin Winona May Hendrata Wulandari, Innah Yoes Priyatna Dachlan YOSHIHIKO YANO YOSHITAKE HAYASHI Yulanda Antonius Yusuf, Muhtarum Zahro, Vida Seanita Zayyin Dinana

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