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All Journal Jurnal Biosains Pascasarjana Journal of Tropical Biodiversity and Biotechnology Jurnal Teknologi Laboratorium Indonesian Journal of Chemistry
Furqoni, Abdul Hadi
Laboratory Of Human Genetic, Institute Of Tropical Disease, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Other

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The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method Masjkur, Indah Nuraini; Arfianti, Evy; Yudianto, Ahmad; Furqoni, Abdul Hadi; A’yun, Qurrota
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): 2020 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.243

Abstract

The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining. The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of <0.005, it can be concluded that there are significant differences in DNA levels at the temperature and storage time treatments of the buccal swab sample. The results of DNA visualization at the FGA and D13S317 loci using the STR PCR method in this study can still be detected and can be used as a reference for examination in forensic cases.
Ectoparasite Infestation among Stray Cats around Surabaya Traditional Market, Indonesia Shifa Fauziyah; Abdul Hadi Furqoni; Norma Farizah Fahmi; Adi Pranoto; Pradika Gita Baskara; Lensa Rosdiana Safitri; Zukhaila Salma
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53687

Abstract

This study was conducted to determine the prevalence of ectoparasite infestation among stray cats around Surabaya traditional markets. A total of 305 stray cats were collected around 17 traditional markets in Surabaya City and were examined for the presence of fleas with a fine-toothed flea comb. Surveys were conducted during May-June 2019. 228 of 305 stray cats (74.75%) were infested with one species of ectoparasite. The average number of C. felis in every cat was 2.54, while the number of F. subrostratus in every cat was 0.33. Additional data about the gender, pregnancy/maternity, and bodyweight of every cat were recorded. The result of chi-square test shows that there is a significant difference between gender, pregnancy status, and bodyweight by the occurrence of ectoparasites (p=0.008; p=0.00; p=0.00). A total of 878 ectoparasites consisting of flea and lices, namely Ctenocephalides felis (88.27%) as the dominant ectoparasite, followed by Felicola subrostratus (11.73%). The highest infection rate (prevalence) of ectoparasite was found in Pucang Market (16.81%), while the lowest prevalence was found in Mulyorejo Market (0.8%). Coinfection was observed in only a few cats (1.63%). Multiple Regression showed that pregnancy is the most influential factor in the occurrence of fleas (p=0.000). These results should be taken into account among health workers to prevent a possible outbreak of zoonotic diseases caused by fleas. 
Pengaruh Rendaman Air Terhadap Kualitas DNA pada Sperma dengan STR-CODIS D13S317 dan D21S1 Abdul Hadi Furqoni; Ahmad Yudianto; Puspa Wardhani
Jurnal Biosains Pascasarjana Vol. 19 No. 1 (2017): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (984.572 KB) | DOI: 10.20473/jbp.v19i1.2017.41-54

Abstract

AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ini bisa menimpa beberapa orang dan dari berbagai umur.  Dalam kasus ini pasti akan di temukan barang bukti di tempat kejadian perkara. Salah satu bukti akibat kejahatanseksual adalah bercak sperma. Bercak sperma dapat di temukan seperti pada pakaian yang digunakan korban atau pelaku. Dengan keterbatasan waktu yang dimiliki oleh polisi tentunyaperlu  cara  untuk  pengidentifikasi  siapa  pelaku  dari  kejahatan  tersebut.  Sampel  bercak sperma akan di isolasi DNA.   Isolasi DNA adalah memisahkan DNA yang ada pada sel sperma dengan komponen yang lainnya. Setelah DNA di dapatkan akan di ketahui kadar dankemurniannya. Hasil tersebut bisa di pengaruhi oleh media bercak sperma berada. Faktor- faktor tersebut salah satunya media air dan kain katun. Sifat kain katun adalah mempunyaikemampuan menyerap yang tinggi walaupun dalam keadaan basah sekalipun.  Setelah isolasi DNA, tahapan selanjutnya adalah amplifikasi. Proses amplifikasi dalam penelitian ini adalah menggunakaan STR dengan lokus D13S317 dan D21S11.Kata kunci : Kriminal, Sperma, DNA, STR-CODIS
Effect of Storage Time on DNA Content and Purity in Lip Print Ahmad Yudianto; Titik Erliyah; Abdul Hadi Furqoni; Indah Nuraini; Qurrota A'yunil Huda
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.43-48

Abstract

Forensic experts in uncovering the truth of a case must go through identification, documentation, and collection of evidence. Sometimes at a crime scene, lip prints are found on the surface of an object such as the mouth of a plastic bottle. Lip print research on plastic bottle mouths was carried out for 1, 3 and 7 days. There are 21 samples with details of 3 as controls, 6 samples for the first day, 6 samples for the 3rd day, and 6 samples for the 7th day. DNA extraction using DNAzol, quantification using UV spectrophotometer, and DNA amplification using STR primers, namely TPOX and TH01 loci. For DNA visualization using acrylamide gel. The average results of lip print DNA levels for 1, 3, and 7 days were 369.82 g/ml, 550.72 g/ml, 318.02 g/ml. The average yield of lip print DNA purity for 1, 3, and 7 days was 1.79; 1.78 and 1.79. From the results of DNA quantification, the lowest and highest DNA levels were taken on days 1, 3, and 7. Of the 6 samples and 3 controls amplified using the TPOX and TH01 loci, the results were clearly visible on the acrylamide gel band.
Limit Detection of Short Tandem Repeats (STR) Analysis on Touch DNA Samples Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari, Novitasari; Furqoni, Abdul Hadi
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.94081

Abstract

Forensic short tandem repeats (STR) profiling on touch DNA samples has emerged as a primary method for human identification. The stability and uniqueness of STR combination from the targeted locus in each individual make it a precision marker for human identification. Touch DNA samples can be found in traces of biological material shed from a person. This work aimed to identify the lowest concentration limit required for generating an interpretable DNA profile and the sensitivity of the STR loci applied. Touch DNA samples were collected from donors who were asked to hold a rope for 5 min. A double swab technique was used to lift the touch samples from the rope. These samples are subjected to DNA extraction and quantification. Two STR amplification cycles, 29 and 34 cycles, were used. DNA concentration greatly influences the success of amplifying the target allele at each STR locus to be interpreted into a complete DNA profile, shown by its allele peak. Touch DNA concentration >0.25 ng can produce a complete DNA profile. LCN method successfully amplified touch DNA with a concentration 0.0625–0.25 ng/µL. Limit detection of touch DNA analysis is 0.25 ng/µL. Low-copy DNA can still be analyzed within 0.0625–0.25 ng/µL.
Co-Authors A’yun, Qurrota Adi Pranoto Ahmad Yudianto Arfianti, Evy Fahmi, Norma Farizah Indah Nuraini Indah Nuraini Masjkur Lensa Rosdiana Safitri Muktiningsih Nurjayadi Novitasari Novitasari Pradika Gita Baskara Puspa Wardhani Qurrota A'yunil Huda Saamia, Vira Shifa Fauziyah Titik Erliyah Zukhaila Salma