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All Journal Jurnal Biosains Pascasarjana Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Majalah Obstetri dan Ginekologi Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Care : Jurnal Ilmiah Ilmu Kesehatan JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service)

Puspa Wardhani

Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Airlangga

Author-ID : 3096214

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia Shifa Fauziyah; Sri Subekti; Budi Utomo; Teguh Hari Sucipto; Hebert Adrianto; Aryati Aryati; Puspa Wardhani; Soegeng Soegijanto
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65357

An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.

RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Yovilianda Maulitiva Untoro; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 1 (2018): Juni
Publisher : Universitas Airlangga

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Rizqidhana Juliana Putri; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.

Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia.

Pengaruh Rendaman Air Terhadap Kualitas DNA pada Sperma dengan STR-CODIS D13S317 dan D21S1 Abdul Hadi Furqoni; Ahmad Yudianto; Puspa Wardhani
Jurnal Biosains Pascasarjana Vol. 19 No. 1 (2017): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ini bisa menimpa beberapa orang dan dari berbagai umur. Dalam kasus ini pasti akan di temukan barang bukti di tempat kejadian perkara. Salah satu bukti akibat kejahatanseksual adalah bercak sperma. Bercak sperma dapat di temukan seperti pada pakaian yang digunakan korban atau pelaku. Dengan keterbatasan waktu yang dimiliki oleh polisi tentunyaperlu cara untuk pengidentifikasi siapa pelaku dari kejahatan tersebut. Sampel bercak sperma akan di isolasi DNA. Isolasi DNA adalah memisahkan DNA yang ada pada sel sperma dengan komponen yang lainnya. Setelah DNA di dapatkan akan di ketahui kadar dankemurniannya. Hasil tersebut bisa di pengaruhi oleh media bercak sperma berada. Faktor- faktor tersebut salah satunya media air dan kain katun. Sifat kain katun adalah mempunyaikemampuan menyerap yang tinggi walaupun dalam keadaan basah sekalipun. Setelah isolasi DNA, tahapan selanjutnya adalah amplifikasi. Proses amplifikasi dalam penelitian ini adalah menggunakaan STR dengan lokus D13S317 dan D21S11.Kata kunci : Kriminal, Sperma, DNA, STR-CODIS

Antimalarial Activity of Ethanol Extract of Kelakai Leaves (Stenochlaena palustris) to Parasitemia and Splenomegaly in BALB/c Mice Infected with Plasmodium berghei ANKA Laily Nur Azizah; Puspa Wardhani; Heny Arwati
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 13 No. 1 (2022): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V13I12022.1-5

Introduction: Malaria is one of global health problems. Splenomegaly is one of malaria symptoms. Antimalarial drug resistance had been reported. Alternative treatment is by using traditional medicinal plants such as kelakai (Stenochlaena palustris). Kelakai contains alkaloid and flavonoid which had been reported to have antimalarial activity. The aim of this study was to discover antimalarial activity of ethanol extract of kelakai leaves to parasitemia and splenomegaly of Plasmodium berghei ANKA in infected BALB/c mice.Methods: This study was based on a modified Peter test using BALB/c mice infected with P. berghei ANKA treated with ethanol extract of kelakai leaves, with chloroquine diphosphate as a positive control. The negative control was P. berghei ANKA infected mice without any additional treatment. Administration of ethanol extract of kelakai leaves was performed for 4 days with a serial doses of 100, 10, and 1 mg/kg body weight. The positive control was given chloroquine diphosphate 20 mg/kg body weight. Parasitemia was observed daily prior to the calculation of the percentage of parasite growth and parasite growth inhibition. At the end of the test, the mice were sacrificed and spleens were isolated to measure their sizes. Probit analysis was performed to obtain ED50 to find the effect of extract in parasite killing by 50%. Spearman test was performed to analyze the correlation of doses of extract and splenomegaly.Results: Parasitemia growth inhibition was directly proportional to the dose. Higher parasitemia inhibition was obtained at higher doses and vice versa. Result of probit analysis showed an ED50 was 77.05 mg/kg body weight. Statistical analysis resulted in insignificant correlation between doses and splenomegaly p = 1.0 (significancy < 0.05).Conclusion: Ethanol extract of kelakai leaves possessed good antimalarial activity and there was no correlation between extract doses and splenomegaly in Plasmodium berghei ANKA-infected mice.

Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an easternpart of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects ofmalaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine.Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein(Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malariatransmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescentinfections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population ofP.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots(DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior toPfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants ofallele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele(12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained ahigher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in thestudied area.

Description of Fecal Culture Results in Diarrhea Patients Due To Antibiotic Use Suci Tresna; I.G.A.A Putri Sri Rejeki; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1448

Diarrhea infection is common in developing countries and causes death of around 3 million people every year. Diarrheais also the second leading cause of death in infants. Riskesdas in 2013 showed 30,775 cases of diarrhea. Causes were such asbacterial infections Salmonella, Shigella, Vibrio, Entamoeba, and Yersinia. Other influences can occur due to viral andfungal infections. Diarrhea is a nosocomial infection that is common in hospitalized patients due to the long-term use ofantibiotics caused by Clostridium difficile. This study was a follow-up study of diarrhea patients who received antibiotictherapy for more than two days with the results of C.difficile negative toxin, then continued with fecal culture examination.This study aimed to look at the description causes of diarrhea other than C.difficile in patients who received long-termantibiotic therapy. This research is an observational study. Samples were taken from 30 diarrhea patients with 2 x 24 hours ofantibiotic use who were hospitalized in the ICU, Dr. Soetomo Hospital Surabaya from August 2017 to May 2018. Sampleswith negative C.difficiletoxin results were then followed by fecal culture examination using conventional methods. Theresults of culture examination from 30 samples showed three samples with positive culture results extended-spectrumβ lactamase producing E.coli, two samples positive culture just E.coli, and 25 other samples showed negative culture results.The results of the fecal culture examination showed a description of causes of diarrhea in patients who received antibiotictherapy was pathogenic E.coli (ESBL). The possibility of other causes that cannot be detected from the culture such as viraland fungal infections, still requires further research.

COMPARISON OF HPV DETECTION USING HC-II METHOD WITH PAP SMEAR SCREENING IN COMMERCIAL SEX WORKERS IN KEDIRI Erawati Erawati; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1336

Female commercial sex workers are females that have multiple sexual partners and have high risk due to exposure to blood, semen, and vaginal discharge contaminated with microorganisms causing sexually transmitted disease such as infection caused by Human papillomavirus (HPV). This behavior creates a high susceptibility for commercial sex workers in obtaining HPV, which is the leading cause of cervical cancer. Cervical cancer is the most common cancer in females in Indonesia, which is why screening, especially for females with a high risk such as commercial sex workers, must be done. The purpose of this experiment was to compare the detection methods of HPV using Hybrid capture-II (HC-II) in order to find out high risks HPV types (type 16, 18, 31, 33, 35, 39,45, 51, 52, 56, 58, 59, and 68) by Pap smear done in commercial sex workers in Campurejo Kediri Public Health Center. This study was a descriptive observational experiment with a cross-sectional method. The samples of this experiment were 47 female commercial sex workers, whose detection of HPV using HC-II method was done at the Clinical Pathology Laboratory of the Dr. Soetomo Hospital Surabaya, where 32 samples showed positive results (68.1%) and were infected with high-risk HPV and 15 negative results (31.9%), from the Pap smear three samples (6.4%) showed dysplasia (Cervical Intraepithelial Neoplasia/CIN 1) and 44 samples (93.6%) showed normal smears with inflammation or infection in the cervix. Statistically showed a significant difference between the results of HC-II and Papsmear (p=0.000).

Diagnostic Value of Plasmotec Malaria-3 Antigen Detection on Gold Standard Microscopy Trieva Verawaty Butarbutar; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1529

Plasmotec Malaria-3 is a rapid malaria diagnostic test that uses four-line tests and targets three malaria proteins,namely Plasmodium falciparum specific protein (HRP-2), Plasmodium vivax-specific LDH (Pv-LDH) and non-specificPlasmodium LDH (pLDH). Microscopy as a gold standard has many disadvantages and the availability of malaria RapidDiagnostic Tests (RDTs) in detecting three proteins is still very limited. This study aimed to determine the diagnostic value of® ® Plasmotec Malaria-3 against gold standard microscopy, comparing the Plasmotec Malaria-3 and microscopy antigen® species detection, determining the Parasitemia Index (PI) cut-off using Plasmotec Malaria-3. This study was across-sectional study with 105 whole blood samples obtained from the Merauke Papua General Hospital which fulfilled theinclusion and exclusion criteria. Samples were examined by thick and thin drops and then examined with Plasmotec®® Malaria-3. Diagnostic values of Plasmotec Malaria-3 against the microscopy were Sn 100%, Sp 98.04%, PPV 98.18%, NPV® 100%, LR + 51, LR-0, diagnostic accuracy of 99.05%. Comparison of Plasmodium species between Plasmotec Malaria-3 and® microscopy was not significantly different, p-value = 0.172. The cut-off of PI in P.falciparum and P.vivax in PlasmotecMalaria-3 based on the Receiver Operating Characteristic (ROC) curve could not be determined with AUC=0.577,p-value=0.385 and AUC=0.423, p-value=0.385, respectively. This study concluded that the comparison of Plasmodium® species between Plasmotec Malaria-3, and microscopy was not significantly different. This study suggested that further® research is needed to find the diagnostic value of non-falciparum and non-vivax Plasmodium against Plasmotec Malaria-3.

Co-Authors Agil Saputra Ahmad Yudianto Alfino Validita Sidiq Anandia Nafisah Putri Arifa Mustika Arifa Mustika Aryati Aryati Aryati Aryati Ashon Sa’adi Audia Mumtaz Rifasky Bagus Setyoboedi Baiq Nasha Islaeli Budi Utomo Desty Indah Sari Dwiyanti Puspitasari, Dwiyanti Erawati Erawati Faizah, Zakiyatul Fauqa Arinil Aulia Fita Triastuti Furqoni, Abdul Hadi Hamdani Lunardhi Harsasi Setyawati Hebert Adrianto Heny Arwati Hermina Novida, Hermina I Dewa Gede Ugrasena I.G.A.A Putri Sri Rejeki Ilham Harlan Amarullah Laily Nur Azizah Maesarah Maesarah Martono Martono Mochammad Reza Desianto Museyaroh, Museyaroh Musholli Himmatun Nabilah Nastasya Nunki Ninik Darsini Novi Ersanto Nyilo Purnami Puspitasari, Yessy Rina Erlina Risa Etika, Risa Rizqidhana Juliana Putri Shifa Fauziyah Shuhai Ueda Siti Churrotin, Siti Siti Khaerunnisa Soegeng Soegijanto Sri Subekti Suci Tresna Teguh Hari Sucipto Teguh Hari Sucipto, Teguh Hari Teguh Satrio Thomas Tandi Manu Tigor Pandapotan Sianturi Trieva Verawaty Butarbutar Ulfa Kholili Uli Mas'uliyah Indarwati Viky Nafi'ah Rahma Maulidia Viky Nafi'ah Rahma Maulidia Widaninggar Rahma Putri Yetti Hernaningsih Yovilianda Maulitiva Untoro Yulia Nadar Indrasari

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