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Journal : Saintia Biologi

UJI POTENSI BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Rhizoctonia solani PENYEBAB REBAH KECAMBAH PADA KENTANG VARIETAS GRANOLA Dewi Novina; Dwi Suryanto; Elimasni D
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

A study on assay the potential of chitinolytic bacterial isolates to inhibit the growth of Rhizoctonia solani causal agent of damping-off on potato was carried out  in Laboratory of Observation Pest and Disease, Medan Johor, UPT. Protection of Crops and Holticulture 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Science of Universitas Sumatera Utara, Medan. Two out of six isolates of chitinolytic bacteria showed capabilities to inhibit of R. solani growth. The most effective isolates to inhibit the growth of R. solani were Enterobacter sp. BK15 and Bacillus sp. BK13, that were able with inhibition zone of 2.05 and 2.35 cm, respectively. These two isolates also showed to be able to reduce fungal infection by 31.25% and 37.5%, respectively.   Keywords: Bacillus sp. BK13, damping-off, Enterobacter sp. BK15, R. solani, potato
SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (Indole Acetic Acid) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (Glycine max L.) Ratna Sari Tarigan; It Jamilah; Elimasni D
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Rizosphere bacteria can be used to fix nitrogen and  produce IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability of rhizosphere bacteria can be improved using biotechnology. The aim of this study is to select the most potential nitrogen fixing and IAA producing bacteria. Nitrogen fixing and IAA producing bacteria were isolated using JNFB and Luria Bertani + L-tryptofan medium respectively. The ability of nitrogen fixing bacteria were tested by ARA method, while the ability of IAA producing bacteria were investigated by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3 which was 33.3 ppm and the highest concentration of nitrogen was yielded by isolate N3 which was 29.93 ppm. Both N3 isolate and I3 isolate potentially as biofertilizer known as PGPR (Plant Growth Promoting Rhizobacteria).   Keywords: nitrogen fixing bacteria, IAA producing bacteria, PGPR
ANALISIS KARIOTIPE DAN PERTUMBUHAN KACANG KEDELAI (Glycine max L.) SETELAH DIINDUKSI SINAR ULTRAVIOLET Rani Ernita Limbong; Elimasni D; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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Abstract

“Karyotype and Growth Analysis of Soybean (Glycine max L.) after Exposed by Ultraviolet light” has been done at Genetic and Molecular Biology Laboratory at Natural Scienes Faculty and the greenhouse at Agriculture Faculty Sumatera Utara University Medan. The objective was to investigate design karyotype and vegetative growth of soybean that induced by UV light. The experimental design was used complete factorial randomized with 2 factors. The first are Ultraviolet (UV) light 3 levels : 10, 20 and 30 Watt and the second is the time of UV exposure with 3 levels : 5, 10, and 15 minutes. Each treatment replicates 2 times and plant that not exposed by UV used as control. The karyotype was observed by squash method. The results showed that the intensity and duration of UV light have no effect on the number of chromosomes but the light has effect on the number of chromosome type. Statistical analysis showed that the increase in UV energy up to 30 Watt and time exposure of 15 minutes, caused the increase in height, number of leaves, number of flowers, the fresh and dry weight of the plant, and chlorophyll concentration compared to untreated plants. Keywords: karyotype, mutation, soybean, ultraviolet.