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PENGARUH PEMBERIAN EKSTRAK DAUN ANDALIMAN (ZANTHOXYLUM ACANTHOPODIUM DC.) TERHADAP HISTOLOGIS OVARIUM MENCIT (MUS MUSCULUS L.) Batubara, Melvariani Syari; Sabri, Emita; Tanjung, Masitta
BIOLINK : Jurnal Biologi Lingkungan Industri Kesehatan Vol 6, No 2 (2020): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i2.2409

Research the effect of andaliman leaf extract on mice ovaries, volume test material using oral gavage needles as much 0.1 ml / 10 g bb 4 days before and after estrus stage and using RAL non-factorial method consisting of control and treatment group. Treatment consisted of factor test material with dose 2500, 5000, 10000, 20000, 40000 ppm. Chemical content of andaliman leaf extract shown from results study are alkaloids, steroids and saponins. Giving treatment of andaliman leaf extract for 9 days on histological data in the number primary, secondary, De Graff, and atretic follicles experienced fluctuating changes, but statistically did not show a significant difference between treatment and control mice groups, while different results were significantly shown in the number of corpus luteum. Parameters diameter of primary follicles, De Graff, and corpus luteum tended to experience changes that fluctuated, but statistically did not show the difference between the treatment and control mice groups, while the diameter of secondary and atretic follicles showed significantly different results. From the results study, administration of andaliman leaf extract affects histological mice ovaries, because it affects oogenesis and ovulation, but fluctuates, possibly due to crude extract, which has not isolated chemical content.

The Utilization of Bunker Eggs into Duck Feed in Tanjung Rejo Village, Deli Serdang Regency Mayang Sari Yeanny; Masitta Tanjung
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2019): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

The community service activities with the title "The Utilization of Bunker Eggs into Duck Feed in Tanjung Rejo Village, Deli Serdang Regency" will be held in May - November 2019. This activity aim is to convert bunker eggs (eggs that failed to hatch) into duck feed which has economic value and is environmentally friendly. The method used is counselling, lectures and training as well as monitoring and evaluation with the community directly to the field.The stages of duck feed production include; (1) choosing a bunker egg (2) egg waste is put into cormorant and boiled for approximately 1 hour, (3) cut water hyacinth/kale, (4) rice bran and water hyacinth/kale steamed into cormorant II which has been filled with water for approximately more than 1 hour, (5) bunker eggs, rice bran, and water hyacinth/kale are removed and drained in large containers and sacks, (6) bunker eggs are mashed without peeling the skin, (7) mixed all ingredients (endhog bunker, bran, kale) by adding enough water and stir until evenly distributed, (8) milling the mixture of materials using a special grinding to form feed, and (9) finishing includes the drying process using an oven/stove. Egg bunkers can be converted into animal feed

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN ANDALIMAN (Zanthoxylum acanthopodium DC.) TERHADAP GAMBARAN MORFOLOGI OVARIUM MENCIT (Mus musculus L.) STRAIN DDW. MELVARIANI SYARI BATUBARA; EMITA SABRI; MASITTA TANJUNG
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1232

A study on the effect of andaliman (Zanthoxylum acanthopodium DC.) leaves ethanol extract to morphological ovary of mice (Mus musculus L.) strain DDW. has been, and was given orally by gavage on 4 days before estrus and 4 days after estrus with the volume given was 0,1 ml/10 g body weight. The research has been studied at Animal Structure And Physiology Laboratory of Department of Biology in Mathematics And Natural Science Faculty, University ofÂ North Sumatera in Medan since February 2006 until September 2006. The research method used was completely randomized designed non factorial which consist of treatment and control groups. The treatment had one factor that is extract dosages with 2500 ppm (0,25 g/100 ml aquadest), 5000 ppm (0,5 g/100 ml aquadest), 10000 ppm (1,0 g/100 ml aquadest), 20000 ppm (2,0 g/100 ml aquadest), 40000 ppm (4,0 g/100 ml aquadest).The result showed that the chemistry consist of andaliman leaves extract is alkaloid, steroid, and saponin. The effect of andaliman leaves extract on 9 days to morphology ofÂ ovary weight and diameter of mice did effect, but DnMRT showed non significantly effect of treatment and control groups of mice. From the result it was concluded that andaliman leaves extract did effect to ovary morphology of female mice, because can be effected oogenesis and ovulation. But the result showed that fluctuatif, because maybe the andaliman extract is crude extract that didnâ€™t Â isolation chemistry consist.

PENGARUH PEMBERIAN EKSTRAK DAUN ANDALIMAN (Zanthoxylum acanthopodium DC.) TERHADAP HISTOLOGIS OVARIUM MENCIT (Mus musculus L.) Melvariani Syari Batubara; Emita Sabri; Masitta Tanjung
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 6, No 2 (2020): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i2.2409

Research the effect of andaliman leaf extract on mice ovaries, volume test material using oral gavage needles as much 0.1 ml / 10 g bb 4 days before and after estrus stage and using RAL non-factorial method consisting of control and treatment group. Treatment consisted of factor test material with dose 2500, 5000, 10000, 20000, 40000 ppm. Chemical content of andaliman leaf extract shown from results study are alkaloids, steroids and saponins. Giving treatment of andaliman leaf extract for 9 days on histological data in the number primary, secondary, De Graff, and atretic follicles experienced fluctuating changes, but statistically did not show a significant difference between treatment and control mice groups, while different results were significantly shown in the number of corpus luteum. Parameters diameter of primary follicles, De Graff, and corpus luteum tended to experience changes that fluctuated, but statistically did not show the difference between the treatment and control mice groups, while the diameter of secondary and atretic follicles showed significantly different results. From the results study, administration of andaliman leaf extract affects histological mice ovaries, because it affects oogenesis and ovulation, but fluctuates, possibly due to crude extract, which has not isolated chemical content.

Anticancer Activity of N-Hexane Extract from Sphagneticola trilobata (L.) J.F Pruski Against MCF-7 Breast Cancer Cell Vivi Mardina; Tisna Harmawan; Halimatussakdiah Halimatussakdiah; Syafruddin Ilyas; Masitta Tanjung
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 1 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6183

Abstract : Sphagneticola trilobata (L.) J.F. Pruski is one of the perennial herbs that is widely used by the national and international community to treat various diseases including cancer. The objective of this study was to assessment the anticancer activity of n-hexane extract of S. trilobata leaves for inhibiting the growth of MCF-7 breast cancer cells in vitro by MTT (microculture tetrazolium salt) method. The n-hexane extract of sernai leaves was obtained from the maceration process of samples that were collected from the Langsa city, Aceh. The cytotoxicity test was carried out by incubating MCF-7 cells which had been exposed to several series of sample levels, viz. 1000; 500; 100; 50; 25; 10; 5 and 1 µg/mL. LC50 values are calculated using probit analysis. The results revealed that the n-hexane extract of S. trilobata leaves was cytotoxic against breast cancer cells (MCF-7) with an LC50 value of 0.037 μg /mL.Abstrak : Sphagneticola trilobata (L.) J.F. Pruski merupakan salah satu tanaman herbal yang digunakan secara luas oleh masyarakat nasional dan internasional untuk mengobati berbagai penyakit termasuk kanker. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker ekstrak n-heksana daun S. trilobata dalam menghambat pertumbuhan sel kanker payudara MCF-7 secara in vitro dengan metode MTT (microculture tetrazolium salt). Ekstrak n-heksana daun sernai diperoleh dari proses maserasi sampel yang dikoleksi dari kota Langsa, Aceh. Uji sitotoksisitas dilakukan dengan menginkubasi sel MCF-7 yang telah dipaparkan beberapa seri kadar sampel yaitu 1000, 500, 100, 50, 25, 10, 5 dan 1 µg/mL. Nilai LC50 dihitung dengan menggunakan analisa probit. Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun S. trilobata bersifat sitotoksik terhadap sel kanker payudara (MCF-7) dengan harga LC50 sebesar 0,037 µg/mL.

PENGARUH BUAH TERONG BELANDA (Solanum betaceum Cav.) TERHADAP JUMLAH ERITROSIT DAN KADAR HEMOGLOBIN MENCIT JANTAN (Mus musculus L.) ANEMIA STRAIN DDW MELALUI INDUKSI NATRIUM NITRIT (NaNO2) Sister Sianturi; Masitta Tanjung; Emita Sabri
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.743 KB)

The effect of terong belanda (Solanum betaceum Cav.) juice on anemia mice DDW strain induced by sodium nitrite has been studied from March 2012 to August 2012 in the Laboratory of Animal Physiology and Laboratory of Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan. This research performed with completely randomized design and was divided in two controls and three treatments. The treatments were divided into three classes concentration of juice such as: 40% (P1), 50% (P2), and 60% (P3) respectively. Each class consisted of six animals. The juice was given orally 0.3ml/day. Results indicated that juice of terong belanda in anemia male mice showed no significantly increased in the number of erythrocytes if compared to either the anemia control or blank control, but significantly increased in haemoglobin levels if compared to anemia control. Keywords : erythrocytes, haemoglobin, Mus musculus, sodium nitrit, terong belanda

PENGARUH EKSTRAK SEGAR DAUN ROSELA (Hibiscus sabdariffa L.) TERHADAP JUMLAH ERITROSIT DAN KADAR HEMOGLOBIN MENCIT JANTAN (Mus musculus L.) ANEMIA STRAIN DDW MELALUI INDUKSI NATRIUM NITRIT (NaNO2) Asmitra Sembiring; Masitta Tanjung; Emita Sabri
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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The purpose of this research is to investigate the effect of fresh leave extract of rosela (Hibiscus sabdariffa L.) on erythrocyte and haemoglobin concentration in anemia male mice (Mus musculus L.) DDW strain induced by sodium nitrite. Completely randomized design (CRD) was used in this experiment, consisting of 5 groups. The first two groups is control induced by NaNO2 for 18 days and the second group is induced by NaNO2 for 18 days and then distilled water for 14 days, and three groups of teratment. The treatment group, divided into three classes of concentration such as; 14 mg/ml (P1), 28 mg/ml (P2) and 42 mg/ml (P3). The treatment group induced by NaNO2 first for 18 days and then healing process by extract of rosela leaves for 14 days. Each class consisted of six experiments animals. The extract was given orally 0.3 ml/day. The results indicate that leave extract affect on increasing of erythrocytes and hemoglobin concentration that have a significantly different (p<0.05) between control and treatments. Keyword: anemic, erythrocytes, haemoglobin, rosela leaves.

PENGARUH KUALITAS DAUN MURBEI Morus multicaulis TERHADAP INDEKS NUTRISI ULAT SUTERA Bombyx mori L. (LEPIDOPTERA:BOMBICIDAE) Maya Murni Chanigo; Masitta Tanjung; Nursal .
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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The effect of mulberry leaf quality on the nutrition index of silk worm was studied. The aim is to determine the effect mulberry leaf (Morus multicaulis) on the nutrition index of silkworm. The experiment used completely randomized design (CRD) with 2 treatments. The first treatment the leaves were taken from Morus multicaulis that cultivated in soil with NPK, Urea and TSP fertilizer. The second the plant were not fertilized at all. Nutrition index of silk worm was observed from instar III to instar V. Each treatment used 20 larvae. The result showed that leaf taken from a fertilized plant has significant nutrition index (p<0,05) on ECD (Efficiency Conversion of Digested Food), ECI (Efficiency Conversion of Ingested Food) in instar IV and V, and AD (Approximate Digesstibility) in instar III, IV and V. However, the GR (Growth Rate) and CR (Consumptio Rate) in instar III, IV and V showed no significant effect (p>0,05). Fertilizer only improves ECD (Efficiency Conversion of Digested Food) in instar IV with 45.45%. ECI (Efficiency Conversion of Ingested Food) with III and IV 15.42% : 30.76%, and the value of AD in instar III, IV and V are 11.49%, 12.16% and 8.38% respectively. Keywords: Morus multicaulis, Bombyx mori L., fertilizer, nutrition index

Type of helminth parasite in Snakehead fish (Channa striata) from Seuneubok Cina, Indra Makmur, Aceh Timur, Indonesia Masitta Tanjung
International Journal of Ecophysiology Vol. 1 No. 1 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Snakehead fish (Channa striata) is one of the freshwater fish in tropical regions such as Asia and Africa, and has high nutrition but is difficult to cultivate. In Indonesia, Snakehead fish are found in paddy fields, swamps and ditches, making them susceptible to parasites. Constraints in Snakehead fish cultivation are caused by the nature of the fish as a predatory, lack of the availability of food and environmental conditions that can affect the growth of the fish. The former paddy fields in Seuneubok Cina of Indra Makmur, Aceh Timur, Indonesia, is found many Snakehead fish. This research aims to determine the types of parasites that infect Snakehead fish. The Snakehead fish was taken using electrofishing gear with purposive sampling method, then dissected and identified the endoparasites which contained in the fish intestines. The research results found three genus of parasites: Pallisentis, Procamallanus and Camallanus.

The Effect of Morus Leaf Quality on Bombyx mori L. Nutrition Index Masitta Tanjung
International Journal of Ecophysiology Vol. 2 No. 02 (2020): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v2i02.4681

The effect of quality mulberry leaf Morus cathayana on nutrition index of silkworm Bombyx mori L. have been conducted . This research was Completely Randomized Design (CRD) by two treatments. The first treatment was mulberry plant that cultivated on soil with Urea, TSP and NPK fertilizers and the second was the plants were not fertilized l. Each treatment was replicate 20 times. The results showed that the addition of Urea, TSP and NPK fertilizer on mulberry has effect (p<0,05) on increasing of the growth rate (GR) of instar III, the consumption rate (CR) of instars III and V, and efficiency of conversion of ingested food (ECI) of instar III and V. In addition, approximate digestibility (AD) of instar III, IV were 20%, 7%, 24%, 40% and then V was 51%, 15%, 21% and 70% respectively.

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