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All Journal MEDISAINS Journal of Health and Nutrition Research
Wardana, Nyoman Gede
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Medicine & Pharmacology Nursing Public Health

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The Potential of Binahong Leaf (Anredera cordifolia) Ethanol Extract on Male Fertility in Mice Exposed to E-Cigarette Smoke Widianti, I Gusti Ayu; Wardana, Nyoman Gede; Widhiartini, Ida Ayu Alit; Satriyasa, Bagus Komang; Antari, Ni Wayan Sukma; Pratiwi, Made Sindy Astri
Journal of Health and Nutrition Research Vol. 5 No. 1 (2026)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v5i1.1055

Abstract

Binahong leaf is known for its strong ability to prevent oxidation due to its active flavonoid compounds. Exposure to electronic cigarette smoke can increase reactive oxygen species (ROS), as a contributing factor to infertility. This study aims to identify the potential of Binahong leaf (Anredera cordifolia) ethanol extract on testosterone levels and spermatogenic cells in adult mice (Mus musculus) exposed to electronic cigarette smoke. A post-test control group design was used, with 28 male Balb/c mice allocated to four groups. The control group received 0.3% CMC, while the three treatment groups were administered Binahong leaf ethanol at doses of 50, 100, and 200 mg/kg body weight, in a volume of 0.1 ml orally each evening for 35 days. All mice were exposed to an electronic cigarette containing 6 mg of nicotine for 20 minutes. On day 36, blood samples were obtained to measure testosterone levels via ELISA. Testicular tissue was observed using Hematoxylin-Eosin staining. A marked elevation in testosterone concentrations and spermatogenic cell count was observed in the group treated with 50 mg/kg BW of Binahong leaf ethanol extract in contrast to the control group (p = 0.014 and 0.008, respectively). Higher doses reduced testosterone/spermatogenesis compared to the optimal dose, potentially implying a toxic or inhibitory effect. Binahong leaf ethanol extract administered at 50 mg/kg BW is effective in increasing testosterone levels and spermatogenic cells in adult mice (Mus musculus) exposed to electronic cigarette smoke.
Protective effects of Gyrinops versteegii leaf ethanol extract on Leydig cells and testosterone under excessive physical activity: an in vivo study Widianti, I Gusti Ayu; Wardana, Nyoman Gede; Widhiartini, Ida Ayu Alit; Pratiwi, Made Sindy Astri; Antari, Ni Wayan Sukma
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Vol. 24 No. 1 (2026)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v24i1.28611

Abstract

Background: Excessive physical activity may lead to overtraining syndrome and increased oxidative stress, which can reduce Leydig cell count and testosterone levels. Gaharu (Gyrinops versteegii) leaf ethanol extract contains strong antioxidants that may counteract oxidative damage. However, the effects of excessive physical activity on Leydig cells and testosterone remain unexplored. Purpose: This study aimed to evaluate the protective effects of Gyrinops versteegii leaf ethanol extract on male reproductive parameters under excessive physical activity conditions. Methods: This experimental study employed a randomized, post-test control-group design. Twenty-five adult male Wistar rats were divided into five groups: normal control, excessive physical activity control, and three treatment groups receiving Gyrinops versteegii leaf ethanol extract at 100, 200, and 400 mg/kg BW. Leydig cell counts and serum testosterone levels were analyzed using ANOVA and correlation tests. Results: The highest Leydig cell count and testosterone level were observed in the 200 mg/kgBW group (28.92 cells and 1.822 ng/mL, respectively). Significant differences in Leydig cell counts were observed among groups (p<0.001), and a positive correlation between Leydig cell number and testosterone levels was observed (p=0.006). Conclusion: Gyrinops versteegii leaf ethanol extract demonstrated protective effects against reproductive impairment induced by excessive physical activity in vivo, with an optimal dose of 200 mg/kgBW.
Co-Authors Bagus Komang Satriyasa I.A.A. Widhiartini Ni Wayan Sukma Antari Pratiwi, Made Sindy Astri Widianti, I Gusti Ayu