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PENGEMBANGAN METODE HPTLC-SPEKTROFOTODENSITOMETRI UNTUK PEMISAHAN SENYAWA RIFAMPISIN, ISONIAZID, PIRAZINAMIDA, DAN ETAMBUTOL Prawiranata, I Putu Hengky; Sugiastana, Bagus Nyoman; Putra, I Putu Krisnantara Wijaya; Dharmapradnyawati, Ni Nyoman Putri; Widhiartini, Ida Ayu Alit; Wirasuta, I Made Agus Gelgel; Widjaja, I Nyoman Kadjeng
Program Kreativitas Mahasiswa - Penelitian PKM-P 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.932 KB)

Abstract

A simple chromatography saparation method for rifampicin, isoniazid, pyrazinamide, and ethambutol by using an impregnated TLC plate with paraffin has been studied. The aim of this study was to develop as simultan separation method of rifampicin, isoniazid, pyrazinamide, and ethambutol. TLC silica gel GF254 was impregnated with paraffin in diethyl ether 10% (v/v). Plate was actived on 110°C, 30 minutes. Some variation of mobile phase was used based on a mixture of ethanol and water. The best chromatographic parameters resulting in use a mixture of ethanol:water (95:5 v/v) were added 5% of glacial acetic acid and 1% of diethylamine as mobile phase. Ethambutol can was derivatized by iodine vapor, but it not detected at a TLC-spektrofotodensitometer, so for a validation only rifampicin, isoniazid, and pyrazinamide. This mobile phase gave good separation with Rs>1 and α>1. The chromatography plates were scanned at 335 nm for rifampicin and at 275 nm for isoniazid and pyrazinamide using a TLC-spektrofotodensitometer. Method validation was includes determining the specificity, LOD, and precision of the method. The method was developed in this study had good validation. Specificity of method was determined by purity factor value, on this method was obtained of purity factor (r(s,m) dan r(m,e))<0,99. The results of this study indicate good precision by intraday and interday assay (RSD<20%) of the method validation. LOD for rifampicin, isoniazid, and pyrazinamide was 15,339  ng,  29,719 ng, and 26,892 ng respectively. The method was developed can be used as a reference for separation of rifampicin, isoniazid, pyrazinamide, and ethambutol in biologis or drug samples. Keywords:     Rifampicin, isoniazid, pyrazinamide, ethambutol, impregnation, derivatization
EFEK SUPLEMEN BESI TERHADAP PENINGKATAN HB DAN INDEK ERITROSIT IBU HAMIL Tangking Widarsa; Wayan Weta; Ida Ayu Alit Widhiartini
ARCHIVE OF COMMUNITY HEALTH Vol 1 No 1 (2012): Juni (2012)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.855 KB) | DOI: 10.24843/ACH.2012.v01.i01.p05

Abstract

To study the e? ect of iron supplement equivalent to 60 mg elemental iron and 0.25 mg folic acid tothe erythrocyte indexes and anemia among pregnant mothers.A pretest-pos! est community trialwas carried out among pregnant mothers who were absent of bleeding history and other blooddisorders and gestation’s age were under 24 weeks. Iron tablets equivalent to 60 mg elementaliron and 0.25 mg folic acid were given to the mothers every day until 8 weeks long. Hb, MCV,MCH, and MCHC were evaluated twice before intervention and a$ er intervention. The changeswere analyzed using t-test and X2 test at 0.05 signi? cance level.Before intervention, almost 78.23% of pregnant women su? ering from iron de? ciency (MCH <27 pg/cell) and 35.28% were anemic (Hb < 11 g/dl). The proportion of su? ering iron de? ciency and anemia signi? cantly decreased a$ er intervention (p < 0.05). The proportion of su? eringiron de? ciency decreased to 27.43% and anemia decreased to 9.35%. The mean of Hb, MCH andMCHC signi? cantly increased a$ er intervention (p < 0.05), but there was no changes on MCV (p> 0.05).More then 2/3 of the pregnant mothers su? ered from iron de? ciency and 1/3 were anemic. Ironsupplement signi? cantly increased the erythrocyte indexes and reduced the prevalence of anemiaamong pregnant mothers. Therefore, it is important to continue iron supplement program and itwill be more e? ective when ? rst introduced at pre-maternal period.
ISOLASI DAN AKTIVITAS ANTIKANKER PINOSTROBIN DARI TEMU KUNCI (Kaempferia pandurata Roxb) TERHADAP FIBROSARKOMA MENCIT HASIL INDUKSI BENZOPIREN Oka Adi Parwata; Sukardiman Sukardiman; Alit Widhiartini
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.998 KB) | DOI: 10.24843/JCHEM.2014.v08.i02.p16

Abstract

Induction of apoptosis and antiangiogenesis effects of Pinostrobin from Kaempferia pandurata Roxb against Fibrosarkoma mice induced with benzopiren have been investigated. Examination begins with taking tissue of fibrosarcoma in mice infected and weighing the fibrosarcoma obtained. Fibrosarcoma tissues were then stored in 10 % formalin. Weighing results showed that pinostrobin oral concentration of 80 mg/kg can inhibit 68.62 % growth of fibrosarcoma. It was also shown that cancer drug (positive control) resulted in 95.95 % inhibition compared to the negative control. This means pinostrobin can potentially be developed as a cancer chemotherapy drug. Patohystology analysis of the fibrosarcoma tissue with HE staining on light microscope with 400x magnification showed many chromatins (polychromatin) which proved the damage caused by the fibrosarcoma cells.  
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP PERTUMBUHAN METHICILLIN RESISTANT Staphylococcus aureus SECARA IN VITRO Cokorda Istri Dyah Yustika Dewi; Desak Ketut Ernawati; Ida Ayu Alit Widhiartini
E-Jurnal Medika Udayana Vol 10 No 2 (2021): Vol 10 No 02(2021): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2021.V10.i2.P15

Abstract

ABSTRAK Staphylococcus aureus merupakan salah satu patogen yang menjadi penyebab utama infeksi nosokomial. Staphylococcus aureus merupakan ancaman serius dikarenakan resistensinya terhadap antibiotik metichillin sehingga disebut juga sebagai Methicillin-resistant Staphylococcus aureus (MRSA). Namun obat pilihan saat ini menimbulkan efek samping yang tidak diinginkan, oleh karena itu perlu dikembangkan sumber lain untuk terapi MRSA. Salah satu tanaman yang potensial untuk MRSA adalah daun cengkeh (Syzygium aromaticum L.). Penelitian ini bertujuan untuk mengetahui aktivitas antibaketri esktrak etanol daun cengkeh (Syzygium aromaticum L.) terhadap pertumbuhan MRSA secara in vitro. Penelitian ini adalah True Experimental Post Test Only Group Design dengan metode disc diffusion untuk menilai zona hambat dari ekstrak dengan konsentrasi 10%, 20%, dan 50% yang dibuat terhadap bakteri MRSA dengan 6 kali pengulangan. Hasil penelitian menunjukan bahwa rerata diameter zona hambat yang dihasilkan oleh konsentrasi 10%, 20%, dan 50% secara berturut-turut yaitu 9,50 ± 0,54 mm, 12,33 ± 0,81 mm, dan 14,66 ± 1,50 mm. berdasarkan hasil penelitian ini, dapat disimpulkan peningkatan konsentrasi esktrak etanol daun cengkeh berpengaruh terhadap diameter zona hambat MRSA yang dihasilkan dari percobaan secara in vitro. Kata kunci : Syzygium aromaticum L., zona hambat, MRSA
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN BELUNTAS (Pluchea IndicaL.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus Pyogenes ATCC 19615 SECARA IN VITRO Dewa Ayu Agung Maya Gayatri; Desak Ketut Ernawati; Ida Ayu Alit Widhiartini
E-Jurnal Medika Udayana Vol 10 No 1 (2021): Vol 10 No 01(2021): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/.MU.2021.V10.i1.P02

Abstract

Daun beluntas(Plucheaindica L.) dinyatakan mengandung metabolit sekunder yang efektif sebagai antiinfeksi. Penyakit infeksi yang disebabkan oleh bakteri Streptococcuspyogenes berdampak serius terhadap perkembangan penyakit faringitisakut hingga endocarditis yang mengancam jiwa. Penelitian ini ditunjukkan untuk membuktikan efektivitas ekstraketanol daun beluntas sebagai antibakteri terhadap S.pyogenes. Uji efektivitas antibakteri ekstraketanol daun beluntas pada konsentrasi 25%,50%,dan75%, dengan kontrol positif Amoksisilin 30µg serta kontrolnegatif etanol96% yang dilakukan terhadap bakteri S.pyogenesATCC 19615 dengan metode cakram disk. Uji fitokimia dilakukan untuk membuktikan adanya kelompok senyawa aktifpada ekstrakuji. Diameterzona hambatdiukur pada hari kedua setelah inkubasibakteri. Diameterzona hambat yangterbentuk pada kelompok konsentrasi 25%,50%,dan75%, secara berurutan adalah 8 mm, 10,5 mm, dan 17,5 mm. Untuk mengetahui adanya efekperlakuan, dilakukan analisis terhadap UjiKruskal-Wallis didapatkan nilaip=0,0001, dan untuk mengetahui adanyakorelasi peningkatan dosisterhadap peningkatan efek antibakteri dilakukan Uji Independent SamplesTest dan Mann Whitney.Ekstraketanol daun beluntas (Plucheaindica L.)memiliki daya hambat pada kadar 25%,50%, dan75% terhadap pertumbuhan bakteri Streptococcus pyogenes ATCC 19615. Kata kunci : Daun Beluntas, ZonaHambat Bakteri, Streptococcus pyogenes
SARI CenTeLLA ASIATICA ASLI BALI MENINGKATKAN SEKRESI TuMouR neCRoSIS FACToR ALPHA (TNF-a) PADA MENCIT YANG DIINFEKSIKAN SALMoneLLA TYPHI I Nyoman Wande; Sianny Herawati; Ida Ayu Alit Widhiartini; I Wayan Putu Sutirta Yasa; Tjokorda Gede Oka; Ni Made Linawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.477

Abstract

Tumour necrosis factor alpha (TNF-α) is a cytokine produced by macrophages and other mononuclear cells, is a good antibacterial agent against Salmonella spp, especially Salmonella typhi. Centella asiatica is an alternative drug that is expected as an immunostimulant in patients with typhoid fever. Comparing the effectiveness of Centella asiatica extract the original Bali as an immunostimulant and without stimulants in mice infected Salmonella typhi in terms of TNF-α secretion. This study is an experimental study with a post test only with control group design. A total of 20 mice were divided into 4 groups. The first and second groups each given Centella asiatica extract 75 mg/20 g bw (0.5 cc) and without a given extract for 4 weeks. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the second week. The third and fourth groups were given thiamphenicol with Centella asiatica extract 75 mg/20 g bw (0.5 cc) and thiamphenicol without any extract for 4 weeks respectively. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the first day. All groups terminated on fourth week and examination levels of TNF-α by ELISA and gall culture. The mean levels of TNF-α in groups (1–4) is 86.10±2.67 pg/mL, 32.81±11.33 pg/mL, 35.87±3.90 pg/mL and 19.21±2.19 pg/mL respectively. Based on the examination of the gall cultures this study showed positive results in the first and second groups, while a negative result on the third and fourth groups. Based on the One way ANOVA analysis on levels of TNF-α, there are significant differences between the first group with the second group (p<0.05), and between the third and fourth groups also found significant differences (p<0.05) increased levels of TNF-α in mice with Salmonella typhi infection given Centella asiatica extract.
PELATIHAN PEMBUATAN SABUN PADAT DAN CAIR BERBAHAN EKSTRAK RIMPANG TEMULAWAK DAN SAMBILOTO PADA IBU-IBU PKK DESA MAMBANG, SELEMADEG TIMUR, TABANAN I.G.A. Artini; A.W. Indrayani; N.W.S. Dewi; I.W. Sumardika; I.G.M.G.S.C. Trapika; I.M. Jawi; B.K. Satriyasa; I.G.M. Aman; A.N. Mahendra; I.A.A. Widhiartini; D.K. Ernawati; N.W. Tianing; N.L. Suriani; I.G. Wiranatha
Buletin Udayana Mengabdi Vol 22 No 2 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i02.p03

Abstract

Temulawak is one of medicinal plants which possess antibacterial activity against two major causes of infection in acne namely Staphylococcus epidermidis and Propionibacterium acnes. The active compounds of temulawak are curcumin and xanthorrhizol essential oil, which possess antibacterial activity as antiseptic and antibiotic. Sambiloto (known as Andrographis paniculate), contains active compound andrographolide, which is also assumed to possess antibacterial activity. This community program was the implementation of our research for community wellbeing, which aimed to raise the knowledge and skill of PKK women at Mambang village, Selemadeg Timur, Tabanan especially in producing liquid and solid soap from natural substances (temulawak and sambiloto extract). The program was conducted by visiting our partner company PT Bali Tangi and training for liquid and solid soap production from temulawak and sambiloto (as one form of temulawak and sambiloto utilization for skin health especially for acne management). We assess participant knowledge improvement by using questionaire before and after conducting the activity. There was an improvement in participant knowledge regarding the production of soap from natural substances (temulawak and sambiloto). In conclusion, this program had increase the knowledge and skill of PKK women at Mambang village regarding liquid and solid soap production from natural substances. Keywords: antiacne, liquid soap, sambiloto, solid soap, temulawak
PEMANFAATAN JAMU HERBAL UNTUK MENINGKATKAN IMUNITAS TUBUH DI ERA PANDEMI COVID-19 I.G.A. Artini; I.G.M.G.S.C. Trapika; N.W.S. Dewi; A.W. Indrayani; I.W. Sumardika; I.M. Jawi; B.K. Satriyasa; I.G.M. Aman; A.N. Mahendra; I.A.A. Widhiartini; D.K. Ernawati
Buletin Udayana Mengabdi Vol 22 No 4 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i04.p02

Abstract

To date, COVID-19 infection still become an important health problem in Indonesia, including Bali. We may concern about this situation since there has been no specific drug or vaccine available against COVID-19 infection. In this new normal era, we should maintain our immunity to prevent the infection. Some herbs potentially possess great effect on maintaining immunity to protect ourselves from COVID-19 infection, such as kunyit, temulawak, jahe, kencur, pegagan and kelor. To date, herbs utilization for health in Banjar Sambahan remains very limited. This is related to the limited skill in processing herbal drink (jamu). This activity aimed to raise participant (PKK women at Banjar Sambahan) knowledge, as well as skills, on producing herbal drinks (jamu/loloh) that potentially increase the immunity during COVID-19 pandemic era. This programme was performed by socialization about herbs utilization for health (especially for immunity), accompanied by training (by video) on producing herbal drink and survey for herbal medicine utilization in community. The results of this programme were video, publication on national journal (SINTA indexed) and presentation on national scientific meeting. It can be concluded that this activity supply knowledge and skill for PKK women at Banjar Sambahan in processing herbal drink for maintaining the immunity against COVID-19 infection. Keywords: covid-19, immune, herbal drink, community service
KESESUAIAN PERESEPAN ANTIBIOTIKA PADA BERBAGAI TINGKAT KEPARAHAN COVID-19 DI PELAYANAN KESEHATAN RUJUKAN Diaz, Leonardo Alpha; Jawi, I Made; Widhiartini, Ida Ayu Alit; Aman, I Gusti Made
E-Jurnal Medika Udayana Vol 12 No 7 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i07.P09

Abstract

COVID-19 telah menghadapkan klinisi dengan sebuah tantangan baru dimana belum ditemukannya terapi definitif dalam penanganan penyakit tersebut hingga tahun 2021. COVID-19 dapat menyebabkan disregulasi daya tahan tubuh pada sebagian pasien, sehingga pasien tersebut menjadi lebih rentan terhadap koinfeksi bakteri. Antibiotika banyak diresepkan sebagai life-saving drugs dalam penanganan/pencegahan infeksi bakteri yang berpotensi meningkatkan morbiditas dan mortalitas pasien COVID-19. Pada penggunaan dalam keadaan darurat, terdapat potensi penggunaan antibiotika menjadi berlebihan dan tidak sesuai dengan pedoman terapi yang ada. Hal tersebut perlu mendapat perhatian lebih sebagai bahan evaluasi dalam pencegahan isu resistensi. Penelitian ini bertujuan untuk mendapatkan gambaran peresepan antibiotika dikaitkan dengan profil keparahan pasien COVID-19. Rancangan penelitian menggunakan desain deskriptif kuantitatif yang pengambilan datanya dilakukan secara retrospektif terhadap sampel rekam medis pasien terkonfirmasi COVID-19 yang dirawat inap pada sebuah rumah sakit rujukan COVID-19 di Provinsi Bali selama tahun 2021. Sampel diambil dengan menggunakan metode simple random sampling. Sebanyak 304 rekam medis dicantumkan dalam penelitian. Data diolah menggunakan statistik deskriptif. Mayoritas pasien berada dalam kelompok umur 25-34 tahun (23,1%), berjenis kelamin perempuan (51,6%) dan termasuk dalam COVID-19 derajat sedang (67,8%). Sebagian besar pasien yang tercatat tidak memiliki komorbid (54,3%). Azithromycin (55,6%), levofloxacin (25,8%), dan imipenem-cilastatin (8,2%) mendominasi peresepan antibiotika dalam penelitian ini. Sebagian besar pasien (62,5%) mendapat peresepan antibiotika yang sesuai dengan Buku Pedoman Tatalaksana COVID-19 Edisi 3. Tidak ditemukan adanya peresepan antibiotika yang masuk ke dalam kategori Reserve berdasarkan ‘AWaRe Classification’ dari WHO. Dapat disimpulkan, mayoritas pasien yang mendapat peresepan antibiotika telah mempertimbangkan buku pedoman yang ada dan klasifikasi aware WHO.
Gambaran Pengetahuan dan Kepatuhan Terapi Pasien Tuberkulosis Paru di Puskesmas Kota Denpasar selama Pandemi COVID-19 Dewi, Kadek Aprilia Sukma; Widhiartini, Ida Ayu Alit; Aman, IGM
E-Jurnal Medika Udayana Vol 12 No 1 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i01.P12

Abstract

Kepatuhan dalam mengonsumsi obat merupakan kunci keberhasilan terapi Tuberkulosis (TB) Paru, dan pengetahuan diketahui sebagai salah satu faktor predisposisi kepatuhan. Keterbatasan pelayanan TB di Puskesmas selama pandemi COVID-19 berisiko pada penurunan pengetahuan dan kepatuhan terapi Obat Anti Tuberkulosis (OAT) pasien TB Paru. Penelitian ini bertujuan untuk menggambarkan pengetahuan dan kepatuhan terapi OAT pasien TB Paru rawat jalan di Puskesmas Kota Denpasar selama pandemi COVID19. Desain penelitian ini mengunakan studi deskriptif observasional potong lintang selama periode MaretAgustus 2021 di lima Puskesmas Kota Denpasar. Pengetahuan dan kepatuhan diukur menggunakan kuesioner pengetahuan yang dibuat peneliti dan Morisky Medication Adherence Scales (MMAS?8). Data diambil secara daring menggunakan aplikasi google form. Berdasarkan tingkat pengetahuan terdapat 37 pasien (73%) memiliki pengetahuan baik, 13 pasien (25%) memiliki pengetahuan cukup, serta 1 pasien (2%) dengan pengetahuan kurang. Sementara berdasarkan tingkat kepatuhan, sebanyak 29 pasien (57%) memiliki kepatuhan tinggi, 19 pasien (37%) memiliki kepatuhan sedang, dan 3 pasien (6%) dengan kepatuhan rendah. Sebagian besar pasien TB Paru rawat jalan di lima Puskesmas Kota Denpasar selama pandemi COVID-19 memiliki pengetahuan yang baik serta kepatuhan yang tinggi. Kata kunci : Tuberkulosis (TB) paru, kepatuhan, pengetahuan.