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All Journal HAYATI Journal of Biosciences Archive of Community Health Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Farmasi Udayana E-Jurnal Medika Udayana Program Kreativitas Mahasiswa - Penelitian JFM (Jurnal Farmasi Malahayati) Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Pharmademica: Jurnal Kefarmasian dan Gizi Eduvest - Journal of Universal Studies
I.A.A. Widhiartini
Bagian Farmasi Kedokteran Fakultas Kedokteran Universitas Udayana
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Medicine & Pharmacology Neuroscience Public Health Social Sciences

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PENGEMBANGAN METODE HPTLC-SPEKTROFOTODENSITOMETRI UNTUK PEMISAHAN SENYAWA RIFAMPISIN, ISONIAZID, PIRAZINAMIDA, DAN ETAMBUTOL Prawiranata, I Putu Hengky; Sugiastana, Bagus Nyoman; Putra, I Putu Krisnantara Wijaya; Dharmapradnyawati, Ni Nyoman Putri; Widhiartini, Ida Ayu Alit; Wirasuta, I Made Agus Gelgel; Widjaja, I Nyoman Kadjeng
Program Kreativitas Mahasiswa - Penelitian PKM-P 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

A simple chromatography saparation method for rifampicin, isoniazid, pyrazinamide, and ethambutol by using an impregnated TLC plate with paraffin has been studied. The aim of this study was to develop as simultan separation method of rifampicin, isoniazid, pyrazinamide, and ethambutol. TLC silica gel GF254 was impregnated with paraffin in diethyl ether 10% (v/v). Plate was actived on 110°C, 30 minutes. Some variation of mobile phase was used based on a mixture of ethanol and water. The best chromatographic parameters resulting in use a mixture of ethanol:water (95:5 v/v) were added 5% of glacial acetic acid and 1% of diethylamine as mobile phase. Ethambutol can was derivatized by iodine vapor, but it not detected at a TLC-spektrofotodensitometer, so for a validation only rifampicin, isoniazid, and pyrazinamide. This mobile phase gave good separation with Rs>1 and α>1. The chromatography plates were scanned at 335 nm for rifampicin and at 275 nm for isoniazid and pyrazinamide using a TLC-spektrofotodensitometer. Method validation was includes determining the specificity, LOD, and precision of the method. The method was developed in this study had good validation. Specificity of method was determined by purity factor value, on this method was obtained of purity factor (r(s,m) dan r(m,e))<0,99. The results of this study indicate good precision by intraday and interday assay (RSD<20%) of the method validation. LOD for rifampicin, isoniazid, and pyrazinamide was 15,339  ng,  29,719 ng, and 26,892 ng respectively. The method was developed can be used as a reference for separation of rifampicin, isoniazid, pyrazinamide, and ethambutol in biologis or drug samples. Keywords:     Rifampicin, isoniazid, pyrazinamide, ethambutol, impregnation, derivatization
EFEK SUPLEMEN BESI TERHADAP PENINGKATAN HB DAN INDEK ERITROSIT IBU HAMIL Tangking Widarsa; Wayan Weta; Ida Ayu Alit Widhiartini
ARCHIVE OF COMMUNITY HEALTH Vol 1 No 1 (2012): Juni (2012)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.855 KB) | DOI: 10.24843/ACH.2012.v01.i01.p05

Abstract

To study the e? ect of iron supplement equivalent to 60 mg elemental iron and 0.25 mg folic acid tothe erythrocyte indexes and anemia among pregnant mothers.A pretest-pos! est community trialwas carried out among pregnant mothers who were absent of bleeding history and other blooddisorders and gestation’s age were under 24 weeks. Iron tablets equivalent to 60 mg elementaliron and 0.25 mg folic acid were given to the mothers every day until 8 weeks long. Hb, MCV,MCH, and MCHC were evaluated twice before intervention and a$ er intervention. The changeswere analyzed using t-test and X2 test at 0.05 signi? cance level.Before intervention, almost 78.23% of pregnant women su? ering from iron de? ciency (MCH <27 pg/cell) and 35.28% were anemic (Hb < 11 g/dl). The proportion of su? ering iron de? ciency and anemia signi? cantly decreased a$ er intervention (p < 0.05). The proportion of su? eringiron de? ciency decreased to 27.43% and anemia decreased to 9.35%. The mean of Hb, MCH andMCHC signi? cantly increased a$ er intervention (p < 0.05), but there was no changes on MCV (p> 0.05).More then 2/3 of the pregnant mothers su? ered from iron de? ciency and 1/3 were anemic. Ironsupplement signi? cantly increased the erythrocyte indexes and reduced the prevalence of anemiaamong pregnant mothers. Therefore, it is important to continue iron supplement program and itwill be more e? ective when ? rst introduced at pre-maternal period.
ISOLASI DAN AKTIVITAS ANTIKANKER PINOSTROBIN DARI TEMU KUNCI (Kaempferia pandurata Roxb) TERHADAP FIBROSARKOMA MENCIT HASIL INDUKSI BENZOPIREN Oka Adi Parwata; Sukardiman Sukardiman; Alit Widhiartini
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.998 KB) | DOI: 10.24843/JCHEM.2014.v08.i02.p16

Abstract

Induction of apoptosis and antiangiogenesis effects of Pinostrobin from Kaempferia pandurata Roxb against Fibrosarkoma mice induced with benzopiren have been investigated. Examination begins with taking tissue of fibrosarcoma in mice infected and weighing the fibrosarcoma obtained. Fibrosarcoma tissues were then stored in 10 % formalin. Weighing results showed that pinostrobin oral concentration of 80 mg/kg can inhibit 68.62 % growth of fibrosarcoma. It was also shown that cancer drug (positive control) resulted in 95.95 % inhibition compared to the negative control. This means pinostrobin can potentially be developed as a cancer chemotherapy drug. Patohystology analysis of the fibrosarcoma tissue with HE staining on light microscope with 400x magnification showed many chromatins (polychromatin) which proved the damage caused by the fibrosarcoma cells.  
PEMISAHAN RIFAMPISIN, ISONIAZID, DAN PIRAZINAMIDA DENGAN KLT TERIMPREGNASI PARAFIN Prawiranata I.P.H.; Widhiartini I.A.A.; Cahyadi K.D.; Wirasuta I.M.A.G.
Jurnal Farmasi Udayana Vol. 2, No. 2, Tahun 2013
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Telah dilakukan pengembangan metode kromatografi untuk pemisahan rifampisin, isoniazid, dan pirazinamida menggunakan pelat KLT terimpregnasi parafin. Penelitian ini bertujuan untuk mengembangkan metode pemisahan rifampisin, isoniazid, dan pirazinamida secara simultan. KLT silika gel 60 GF254 diimpregnasi menggunakan parafin 10% (v/v) dalam dietil eter. Beberapa variasi fase gerak yang dioptimasi mengacu pada campuran etanol : air (70:30 v/v) + 5% asam asetat glasial + 1% amonia 37%. Parameter kromatografi terbaik diperoleh dari pemisahan menggunakan fase gerak campuran etanol : air (95:5 v/v) + 5% asam asetat glasial + 1% dietilamin. Fase gerak ini juga memberikan pemisahan terbaik untuk ketiga senyawa dengan Rs > 1 dan ? > 1. Pelat dipindai pada panjang gelombang 335 nm untuk analisis rifampisin dan 275 nm untuk analisis isoniazid dan pirazinamida menggunakan KLT-spektrofotodensitometer. Metode yang dikembangkan dapat digunakan sebagai acuan untuk pemisahan rifampisin, isoniazid, dan pirazinamida pada sampel obat ataupun sampel biologis.
PENGARUH HOME CARE TERHADAP PEMAHAMAN DAN KETAATAN PADA PASIEN TUBERKULOSIS DI FARMASI KOMUNITAS Kangagung, C. P.; Susanti, N. M. P; Widhiartini, I. A. A.
Jurnal Farmasi Udayana Vol. 3, No. 2, Tahun 2014
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Tuberkulosis paru  merupakan penyakit menular dengan pengobatan jangka panjang yang memerlukan pelayanan kefarmasian home care oleh apoteker untuk meningkatkan keberhasilan pengobatan. Penelitian ini bertujuan untuk mengetahui pengaruh home care terhadap pemahaman dan ketaatan pasien tuberkulosis paru di farmasi komunitas. Penelitian dilihat melalui survei dengan kuesioner mengenai pengaruh pelaksanaan home care terhadap pemahaman dan ketaatan pasien dalam penggunaan obat.Penelitian melibatkan 15 pasien tuberkulosis paru dari puskesmas Denpasar Selatan I, Denpasar Barat II, Denpasar Timur I dan Denpasar Utara I yang terlibat selama bulan Agustus-Oktober 2013 yang bersedia mengikuti home care. Pasien kemudian diikuti perkembangannya baik melalui kunjungan ke rumah ataupun melalui telepon sebanyak 2 kali seminggu selama 2 bulan. Evaluasi dilakukan dengan pengisian kuesioner sebelum pelaksanaan home care (pretest) dan setelah pelaksanaan home care (posttest). Hasil kuesioner dianalisis menggunakan uji Wilcoxon dengan taraf kepercayaan 95%. Didapatkan hasil nilai signifikansi sebesar 0,002 (p<0,05) pada evaluasi pemahaman pasien yang berarti pelaksanaan home care berpengaruh terhadap pemahaman pasien terhadap penyakit tuberkulosis paru. Namun didapat hasil nilai signifikansi sebesar 0,132 (p>0,05) pada evaluasi ketaatan pasien yang berarti pelaksanaan home care tidak berpengaruh terhadap ketaatan pasien terhadap pengobatannya.
PEMANFAATAN SIMULASI FARMAKOKINETIK DALAM MEMPREDIKSI PROFIL KRONOFARMAKOKINETIK PARASETAMOL PADA PEMBERIAN BERULANG Jaya M.K.A; Susanti N.M.P; Widhiartini I.A.A; Wirasuta I M.A.G.
Jurnal Farmasi Udayana Vol. 2, No. 2, Tahun 2013
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Telah dilakukan simulasi profil kronofarmakokinetik parasetamol setelah diberikan berulang melalui rute oral dari data publikasi kronofarmakokinetik parasetamol. Simulasi dilakukan menggunakan bantuan Micromath Scientist Software 2,01 melalui fitting. Tujuan penelitian ini memperoleh gambaran perubahan profil farmakokinetik parasetamol pada fenomena kronofarmakokinetik setelah pemberian berulang. Tidak terdapat perbedaan antara parameter farmakokinetik publikasi dengan hasil fitting. Parameter farmakokinetik parasetamol hasil fitting dapat digunakan untuk memodelkan profil kronofarmakokinetik parasetamol. Hasil simulasi dapat menggambarkan profil parasetamol yang dipengaruhi oleh fenomena kronofarmakokinetik.
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP PERTUMBUHAN METHICILLIN RESISTANT Staphylococcus aureus SECARA IN VITRO Cokorda Istri Dyah Yustika Dewi; Desak Ketut Ernawati; Ida Ayu Alit Widhiartini
E-Jurnal Medika Udayana Vol 10 No 2 (2021): Vol 10 No 02(2021): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2021.V10.i2.P15

Abstract

ABSTRAK Staphylococcus aureus merupakan salah satu patogen yang menjadi penyebab utama infeksi nosokomial. Staphylococcus aureus merupakan ancaman serius dikarenakan resistensinya terhadap antibiotik metichillin sehingga disebut juga sebagai Methicillin-resistant Staphylococcus aureus (MRSA). Namun obat pilihan saat ini menimbulkan efek samping yang tidak diinginkan, oleh karena itu perlu dikembangkan sumber lain untuk terapi MRSA. Salah satu tanaman yang potensial untuk MRSA adalah daun cengkeh (Syzygium aromaticum L.). Penelitian ini bertujuan untuk mengetahui aktivitas antibaketri esktrak etanol daun cengkeh (Syzygium aromaticum L.) terhadap pertumbuhan MRSA secara in vitro. Penelitian ini adalah True Experimental Post Test Only Group Design dengan metode disc diffusion untuk menilai zona hambat dari ekstrak dengan konsentrasi 10%, 20%, dan 50% yang dibuat terhadap bakteri MRSA dengan 6 kali pengulangan. Hasil penelitian menunjukan bahwa rerata diameter zona hambat yang dihasilkan oleh konsentrasi 10%, 20%, dan 50% secara berturut-turut yaitu 9,50 ± 0,54 mm, 12,33 ± 0,81 mm, dan 14,66 ± 1,50 mm. berdasarkan hasil penelitian ini, dapat disimpulkan peningkatan konsentrasi esktrak etanol daun cengkeh berpengaruh terhadap diameter zona hambat MRSA yang dihasilkan dari percobaan secara in vitro. Kata kunci : Syzygium aromaticum L., zona hambat, MRSA
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN BELUNTAS (Pluchea IndicaL.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus Pyogenes ATCC 19615 SECARA IN VITRO Dewa Ayu Agung Maya Gayatri; Desak Ketut Ernawati; Ida Ayu Alit Widhiartini
E-Jurnal Medika Udayana Vol 10 No 1 (2021): Vol 10 No 01(2021): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/.MU.2021.V10.i1.P02

Abstract

Daun beluntas(Plucheaindica L.) dinyatakan mengandung metabolit sekunder yang efektif sebagai antiinfeksi. Penyakit infeksi yang disebabkan oleh bakteri Streptococcuspyogenes berdampak serius terhadap perkembangan penyakit faringitisakut hingga endocarditis yang mengancam jiwa. Penelitian ini ditunjukkan untuk membuktikan efektivitas ekstraketanol daun beluntas sebagai antibakteri terhadap S.pyogenes. Uji efektivitas antibakteri ekstraketanol daun beluntas pada konsentrasi 25%,50%,dan75%, dengan kontrol positif Amoksisilin 30µg serta kontrolnegatif etanol96% yang dilakukan terhadap bakteri S.pyogenesATCC 19615 dengan metode cakram disk. Uji fitokimia dilakukan untuk membuktikan adanya kelompok senyawa aktifpada ekstrakuji. Diameterzona hambatdiukur pada hari kedua setelah inkubasibakteri. Diameterzona hambat yangterbentuk pada kelompok konsentrasi 25%,50%,dan75%, secara berurutan adalah 8 mm, 10,5 mm, dan 17,5 mm. Untuk mengetahui adanya efekperlakuan, dilakukan analisis terhadap UjiKruskal-Wallis didapatkan nilaip=0,0001, dan untuk mengetahui adanyakorelasi peningkatan dosisterhadap peningkatan efek antibakteri dilakukan Uji Independent SamplesTest dan Mann Whitney.Ekstraketanol daun beluntas (Plucheaindica L.)memiliki daya hambat pada kadar 25%,50%, dan75% terhadap pertumbuhan bakteri Streptococcus pyogenes ATCC 19615. Kata kunci : Daun Beluntas, ZonaHambat Bakteri, Streptococcus pyogenes
SARI CenTeLLA ASIATICA ASLI BALI MENINGKATKAN SEKRESI TuMouR neCRoSIS FACToR ALPHA (TNF-a) PADA MENCIT YANG DIINFEKSIKAN SALMoneLLA TYPHI I Nyoman Wande; Sianny Herawati; Ida Ayu Alit Widhiartini; I Wayan Putu Sutirta Yasa; Tjokorda Gede Oka; Ni Made Linawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.477

Abstract

Tumour necrosis factor alpha (TNF-α) is a cytokine produced by macrophages and other mononuclear cells, is a good antibacterial agent against Salmonella spp, especially Salmonella typhi. Centella asiatica is an alternative drug that is expected as an immunostimulant in patients with typhoid fever. Comparing the effectiveness of Centella asiatica extract the original Bali as an immunostimulant and without stimulants in mice infected Salmonella typhi in terms of TNF-α secretion. This study is an experimental study with a post test only with control group design. A total of 20 mice were divided into 4 groups. The first and second groups each given Centella asiatica extract 75 mg/20 g bw (0.5 cc) and without a given extract for 4 weeks. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the second week. The third and fourth groups were given thiamphenicol with Centella asiatica extract 75 mg/20 g bw (0.5 cc) and thiamphenicol without any extract for 4 weeks respectively. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the first day. All groups terminated on fourth week and examination levels of TNF-α by ELISA and gall culture. The mean levels of TNF-α in groups (1–4) is 86.10±2.67 pg/mL, 32.81±11.33 pg/mL, 35.87±3.90 pg/mL and 19.21±2.19 pg/mL respectively. Based on the examination of the gall cultures this study showed positive results in the first and second groups, while a negative result on the third and fourth groups. Based on the One way ANOVA analysis on levels of TNF-α, there are significant differences between the first group with the second group (p<0.05), and between the third and fourth groups also found significant differences (p<0.05) increased levels of TNF-α in mice with Salmonella typhi infection given Centella asiatica extract.
PELATIHAN PEMBUATAN SABUN PADAT DAN CAIR BERBAHAN EKSTRAK RIMPANG TEMULAWAK DAN SAMBILOTO PADA IBU-IBU PKK DESA MAMBANG, SELEMADEG TIMUR, TABANAN I.G.A. Artini; A.W. Indrayani; N.W.S. Dewi; I.W. Sumardika; I.G.M.G.S.C. Trapika; I.M. Jawi; B.K. Satriyasa; I.G.M. Aman; A.N. Mahendra; I.A.A. Widhiartini; D.K. Ernawati; N.W. Tianing; N.L. Suriani; I.G. Wiranatha
Buletin Udayana Mengabdi Vol 22 No 2 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i02.p03

Abstract

Temulawak is one of medicinal plants which possess antibacterial activity against two major causes of infection in acne namely Staphylococcus epidermidis and Propionibacterium acnes. The active compounds of temulawak are curcumin and xanthorrhizol essential oil, which possess antibacterial activity as antiseptic and antibiotic. Sambiloto (known as Andrographis paniculate), contains active compound andrographolide, which is also assumed to possess antibacterial activity. This community program was the implementation of our research for community wellbeing, which aimed to raise the knowledge and skill of PKK women at Mambang village, Selemadeg Timur, Tabanan especially in producing liquid and solid soap from natural substances (temulawak and sambiloto extract). The program was conducted by visiting our partner company PT Bali Tangi and training for liquid and solid soap production from temulawak and sambiloto (as one form of temulawak and sambiloto utilization for skin health especially for acne management). We assess participant knowledge improvement by using questionaire before and after conducting the activity. There was an improvement in participant knowledge regarding the production of soap from natural substances (temulawak and sambiloto). In conclusion, this program had increase the knowledge and skill of PKK women at Mambang village regarding liquid and solid soap production from natural substances. Keywords: antiacne, liquid soap, sambiloto, solid soap, temulawak
Co-Authors A.N. Mahendra A.W. Indrayani Aman, I Gusti Made Aman, IGM Arifin, Jeanan Aulia Bagus Komang Satriyasa Bagus Nyoman Sugiastana Bayu Mayura, I Putu Cahyadi K.D. Cokorda Istri Dyah Yustika Dewi Desak Ketut Ernawati Dewa Ayu Agung Maya Gayatri Dewi, Kadek Aprilia Sukma Dewi, Putu Diva Candra Diaz, Leonardo Alpha Diksha, I Gusti Ngurah Ariestha Satya I Gusti Ayu Artini I Made Agus Gelgel Wirasuta I Made Jawi I Made Pande Dwipayana I Nyoman Kadjeng Widjaja I Nyoman Wande I Putu Hengky Prawiranata I Putu Krisnantara Wijaya Putra I Wayan Putu Sutirta Yasa I Wayan Sumardika I Wayan Weta I.G. Wiranatha I.G.A. Artini I.G.M. Aman I.G.M.G.S.C. Trapika Ivana, Jocelyn Jawi , I Made Jaya M.K.A K Tangking Widarsa Kangagung, C. P. Made Agus Hendrayana Made Wihandani, Desak Medinna, Balqish Fathoum N.W. Tianing N.W.S. Dewi Nathasia Ni Luh Suriani Ni Made Linawati Ni Nyoman Putri Dharmapradnyawati Ni Putu Tesi Maratni, Ni Putu Tesi Nila Wahyuni Oka Adi Parwata Pandansari, Ni Wayan Bunga Pebrianti, Ni Putu Wika Pratama, Putu Yudha Kartika Prawiranata I.P.H. Ricardo, I Komang Aan Adi Sakaningrum, Ni Putu Mirah Sianny Herawati Stefani, Lidwina Sukardiman Susanti N.M.P Susanti, N. M. P Tjokorda Gede Oka Trapika, I Gusti Made Gde Surya Chandra Wuga, Kristina Supartin Monika