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All Journal International Journal of Public Health Science (IJPHS) Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Damianus Journal of Medicine Indonesian Journal of Tropical and Infectious Disease Jurnal Abdi Insani JURNAL INOVASI KESEHTAN MASYARAKAT Jurnal Laboratorium Khatulistiwa Journal of Community Empowerment
Nina Difla Muflikhah
Program Studi DIII Teknologi Bank Darah Stikes Rajekwesi Bojonegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences

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An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant GRA1 protein for detection of igg antibodies againts Toxoplasma gondii infections Nina Difla Muflikhah; Wayan Tunas Artama
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 2 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.489 KB) | DOI: 10.19106/JMedSci005102201904

Abstract

Reliable laboratory testing is important to detect Toxoplasma gondii infection and focuses on improving the low cost and easy to use the diagnostic instrument. Enzyme-linked immunosorbent assay (ELISA) method can be used to determine a large number of samples within a short period of time which based on antibody or antigen detection. We tested the sensitivity and specificity of GRA1 protein of as antigen using the ELISA method to toxoplasmosis diagnosis and compared it with commercial ELISA kit. Seventy sera samples were collected and tested using indirect ELISA, commercial ELISA kit and GRA1 protein-coated as antigen. The results showed 48 and 51 samples had positive IgG antibody using ELISA-GRA1 and commercial ELISA kit, respectively. The GRA1 sensitivity and specificity on ELISA were 100% and 86.36%, respectively. Whereas positive predictive value (PPV) was 94.11%. This result indicated that the recombinant GRA1 protein is a highly immunogenic protein in human toxoplasmosis and marker for toxoplasmosis screening.
PEMERIKSAAN GOLONGAN DARAH SISTEM ABO RHESUS PADA MAHASISWA STIKES RAJEKWESI BOJONEGORO Fatia Rizki Nuraini; Nina Difla Muflikhah; Siti Nurkasanah
Jurnal Abdi Insani Vol 9 No 2 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i2.566

Abstract

Blood Type is an important thing to every person to understand and can identify using a specific methods, blood typing. Blood typing refers to a specific pattern of reaction to testing antisera within a given system. Blood typing have several methods, the common method that usually used is Direct Slide Test. Over a period of time, our understanding on blood groups has evolved to encompass not only transfusion-related problems but also specific disease association with RBC surface antigens. This community service activity aimed to provide blood typing examination for students who unknown their blood type and strenghten the understanding about the important of Blood Type. This activity was held on December 20, 2021 in Stikes Rajekwesi Bojonegoro. This Community service applied an examination test to several students who still unknown their blood type. Examination of blood group used the A-B-O system and rhesus with the slide test method. The results of the blood group examination of 16 students showed that the highest results were blood group B as many as 6 people (37.5%) and the least blood group being O and AB with 3 people each (18.75). Rhesus examination showed that all students were rhesus positive (100%). Students who examinated during the programmes doesn’t have undestanding of the important of knowing their blood type. Students of Stikes Rajekwesi Bojonegoro get sufficient understanding about the important of knowing their blood type and the blood typing showed 37,5% of student are B type, 18,75% are O and AB type each, while 100% rhesus positive.
AN EVALUATION STUDY OF ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) USING RECOMBINANT PROTEIN GRA1 FOR DETECTION OF IgG ANTIBODIES AGAINTS TOXOPLASMA GONDII INFECTIONS Muflikhah, Nina Difla; Artama, Wayan Tunas
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 5 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.966 KB) | DOI: 10.20473/ijtid.v6i5.5903

Abstract

Toxoplasmosis is an infectious disease caused by Toxoplasma gondii, an intracellular protozoan parasite that live inside the cells of the reticulo endothelial and parenchymal cells of human and animals (mammals and birds). Some cases of toxoplasmosis usually have no symptoms, but in any cases caused severe symptoms, such as hydrocephalus, microcephalus, intracranial calcification, retinal damage, brain abscess, mental retardation, lymphadenopathy, and others. Its severe symptoms usually showed a long time after first exposure, except symptoms showed by congenital transmission caused by infected mother. Early diagnosis is important to prevent the illness but methods for toxoplasmosis screening are still too expensive for developing country. Enzyme-linked immunosorbent assay (ELISA) allow the testing of a large number samples within short time frame and based on antibody or antigen detection. This study aimed to know the sensitivity and specificity of recombinat protein GRA1 as antigen using ELISA methods. We tested the sensitivity and spesificity of GRA1 protein as antigen in ELISA methods to diagnose toxoplasmosis and compared with ELISA Kit Commercial. Reliable laboratory testing is important to detect Toxoplasma gondii infection, and focused to improving the low cost and easy-to-use diagnostic instrument. Seventy sera collected and tested using both indirect ELISA, commercial ELISA kit and GRA1 protein coated as antigen. Fourty eight and fifty one samples showed positive IgG antibody result of ELISA-GRA1 and ELISA kit. Negative sample tested by ELISA-GRA1 was 22 samples and 19 sample tested by ELISA Kit. The sensitivity and specificity of GRA1-based on ELISA were 100% and 86.36%, positive prediction value (ppv) was 94.11%. These data indicate that the recombinant protein GRA1 is a highly immunogenic protein in human toxoplasmosis and become a promising marker for the screening of toxoplasmosis.
Larvacidal Activity of the Mulberry (Morus alba L.) Leaf Extract Against Larvae of Aedes aegypti Muflikhah, Nina Difla
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 2 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i2.37328

Abstract

Dengue Haemorrhagic Fever (DHF) is one of the major public health problems in Indonesia. As the population density increases, the number of sufferers increases. Aedes aegypti mosquitoes are vectors for the disease. The absence of drugs make the best prevention effort by eradicating mosquito nests, killing larvae and adult mosquitoes. Mulberry leaves (Morus alba L.) may be used as larvacides in the presence of chemical compounds of flavonoids and saponins that inhibit feeding and disrupt the process of insect metabolism. The purpose of this research has to determine the effect of mulberry leaf extract (Morus alba L.), to determine the larvicide effect of mulberry leaf extract (Morus alba L.) and to determine the concentration of mulberry leaf extract (Morus alba L.) which is optimal in killing third instar Aedes aegypti larvae. This research used Randomized Design Group (RDG) method with treatment consisted 4 concentrations (0.25%, 0.5%, 0.75%, and 1%), negative control and positive control (ABATE) with 6 repetitions. The results of probit analysis showed that LC50 values were 1.124% and LC90 was 4.413%. From the one way ANOVA test at each concentration of 0.25%, 0.5%, 0.75%, and 1%, the F count result is 208.331, the value was greater than F table which is 2.53 and the significant value is 0.000 (sig <0.05) then mulberry leaf extract (Morus alba L.) has a affected to eliminated of Aedes aegypti larvae. Conclusion from the results of the one way ANOVA test of mulberry leaf extract (Morus alba L.) was affected to eliminated third instar Aedes aegypti larvae
Produksi Komponen Darah Packed-Red Cell (PRC), Liquid Plasma (LP), Thombocytes Concentrates (TC) dan Fresh-Frozen-Plasma (FFP) di UDD PMI Kabupaten Bojonegoro Muflikhah, Nina Difla; Nuraini, Fatia Rizki; Anggita, Etri Sukma
Jurnal Inovasi Kesehatan Masyarakat Vol 4 No 2 (2023): JURNAL INOVASI KESEHATAN MASYARAKAT
Publisher : Fakultas Kesehatan Masyarakat, Institut Kesehatan Deli Husada Deli Tua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36656/jikm.v4i2.1419

Abstract

The processing of blood components is an act of separating the components of donor blood through a process by taking into account the quality and safety of blood component products. Its process produce various type of blood component, such as Packed Red Cell, Liquid Plasma, Thrombocytes Concentrate and Fresh Frozen Plasma. The quantity of each blood production depends on the mount of demant from hospital to threat the patient for transfusion. Indonesia Red Cross especially in Blood Donor Unit of each region have a important duty to collect and process the whole blood component as much as it needed. This type of research is a descriptive study with a cross sectional approach. The population used was the production of PRC, LP, TC and FFP at UDD PMI Bojonegoro from June to July 2022 as many as 2124 kolf. The sampling technique used purposive sampling. Data analysis using univariate analysis which is presented in the form of a frequency distribution table and ghraphic for daily production. Production of blood components as much as 2124 kolf, Production of PRC as 984 kolf (46%), LP as 904 kolf (43%0, TC as 200 kolf (9%), and FFP as 36 kolf (2%). The highest blood component production is PRC, followed by LP, TC and FFP, respectfully.
Produksi Komponen Darah Packed-Red Cell (PRC), Liquid Plasma (LP), Thombocytes Concentrates (TC) dan Fresh-Frozen-Plasma (FFP) di UDD PMI Kabupaten Bojonegoro Muflikhah, Nina Difla; Nuraini, Fatia Rizki; Anggita, Etri Sukma
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1163

Abstract

The processing of blood components is an act of separating the components of donor blood through a process by taking into account the quality and safety of blood component products. Its process produce various type of blood component, such as Packed Red Cell, Liquid Plasma, Thrombocytes Concentrate and Fresh Frozen Plasma. The quantity of each blood production depends on the mount of demant from hospital to threat the patient for transfusion. Indonesia Red Cross especially in Blood Donor Unit of each region have a important duty to collect and process the whole blood component as much as it needed. This type of research is a descriptive study with a cross sectional approach. The population used was the production of PRC, LP, TC and FFP  at UDD PMI Bojonegoro from June to July 2022 as many as 2124 kolf. The sampling technique used purposive sampling. Data analysis using univariate analysis which is presented in the form of a frequency distribution table and ghraphic for daily production. Production of blood components as much as 2124 kolf, Production of PRC as 984 kolf (46%), LP as 904 kolf (43%0, TC as 200 kolf (9%), and FFP as 36 kolf (2%). The highest blood component production is PRC, followed by LP, TC and FFP, respectfully.  
An increase of human immunodeficiency virus infection amongs blood donor during COVID-19 pandemic Muflikhah, Nina Difla; Nuraini, Fatia Rizki
International Journal of Public Health Science (IJPHS) Vol 12, No 3: September 2023
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v12i3.22718

Abstract

Human immunodeficiency virus (HIV) is a one of crucial concern to blood safety because of their prolonged presence in the blood. Unsafe blood transfusion from infected donor contribute to spread the virus as well. Indonesia health ministry reported that HIV infection increase significantly in 2019 to 2021, and HIV case diagnose in population still way from the prediction in 2020, only 78.7% cases reported from 90% prediction case. A retrospective cross-sectional study was conducted and collected 51,351 blood donors during COVID-19 pandemic, 2020-2021. Blood sample analyzed using serological methods to detect the present of HIV antibodies. A reactive result of HIV screening from 21,260 donor in 2020 showed 12 donors reactive (0.00056%), while in 2021 from 30,091 donor showed 42 donors reactive (0.142899%) and showed a significant different from 2020 and 2021 (p-value <0.05). The number of reactive donors increase numerously from 2020 to 2021, while total donor also increased. The level of social restriction has contribution of HIV reactive case among blood donor and showed from two years of pandemic with p-value <0.05. A significant increase of HIV infection among blood donor and the effect of restriction level to prevent COVID-19 transmission are clearly show in this study.
Analysis of Teenagers as Voluntary and Potential Blood Donors at the Blood Donation Unit PMI Bojonegoro Qosiani, Elfa Jiril; Muflikhah, Nina Difla; Nuraini, Fatia Rizki
Bahasa Indonesia Vol 23 No 2 (2024): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v23i2.3462

Abstract

Pendahuluan: Donor darah merupakan kegiatan yang berhubungan langsung dengan keselamatan, kesehatan, dan nyawa, baik bagi pendonor sendiri maupun resipien, Pemenuhan kebutuhan darah sesuai jumlah yang dibutuhkan sangat diperlukan untuk mendukung sistem Kesehatan, terutama selama pandemi COVID-19 karena dipercaya bahwa produk plasmafesesis merupakan salah satu tindakan yang mampu menurunkan gejala pada pasien. Tingginya minat donor darah pada usia remaja di dasari oleh jiwa sosial dan motivasi yang tinggi untuk saling tolong menolong sehingga remaja dikategorikan sebagai pendonor darah potensial dan berkelanjutan. Metode : Penelitian ini dilakukan di UDD PMI Kabupaten Bojonegoro pada Februari  hingga Maret 2022. Desain penelitian yang digunakan yaitu penelitian deskriptif kuantitatif. Pengambilan sampel menggunakan metode purposive sampling dan diperoleh 668 responden, yang terdiri dari 449 donor laki-laki dan 179 donor perempuan. Hasil: Total pendonor darah yang terkumpul selama periode Februari – Maret 2022 sebanyak 668 donor (449 laki-laki dan 179 perempuan). Menurut WHO, batas usia pendonor adalah usia remaja hingga lansia (sebelum 65 tahun). Analisis data menunjukkan 181 donor (27%) termasuk kategori remaja (17-25 tahun), 167 donor (25%) kategori dewasa awal (26-35 tahun), 167 donor (25%) kategori dewasa akhir (36-45 tahun), 124 donor (19%) kategori lansia awal (46-55 tahun), dan 29 donor (4%) kategori lansia akhir (56-65 tahun). Beberapa karakteristik yang memengaruhi keberhasilan donor darah pada kelompok usia remaja antara lain kadar hemoglobin, tekanan darah, dan konsumsi makanan <2 jam sebelum donor. Simpulan: Penelitian ini disimpulkan bahwa presentasi keberhasilan terbanyak adalah pada remaja akhir sebanyak 181 (27%). Proses seleksi donor pada kelompok usia remaja dipengaruhi oleh kadar hemoglobin, tekanan darah, dan waktu terakhir makan.
IMPLEMENTASI PROGRAM GEROBAK SEBAGAI STRATEGI PEMBENTUKAN DESA SIAGA DONOR BERBASIS KELUARGA Nuraini, Fatia Rizki; Muflikhah, Nina Difla; Astuti, Retno Puji
Journal of Community Empowerment Vol 4, No 1 (2025): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jce.v4i1.31486

Abstract

ABSTRAK                                                                            Ketersediaan darah dalam situasi darurat masih menjadi tantangan serius di berbagai daerah, terutama di tingkat desa yang jauh dari fasilitas transfusi darah. Program GEROBAK (Gerakan Donor Bersama Keluarga) hadir sebagai solusi untuk memberdayakan keluarga dalam membentuk sistem donor darah mandiri dengan meningkatkan pengetahuan dan kesadaran tentang pentingnya donor darah. Kegiatan pengabdian masyarakat ini dilaksanakan di Desa Prayungan dengan melibatkan kerjasama aktif antara tim pelaksana dan pihak pemerintah desa. Program ini diikuti oleh 30 orang peserta, terdiri dari perwakilan keluarga. Metode pelaksanaan terdiri atas tiga tahap, yaitu: persiapan dan koordinasi, sosialisasi dan edukasi, serta monitoring dan evaluasi. Hasil pretest dan posttest menunjukkan adanya peningkatan signifikan dalam pengetahuan peserta, yaitu sebesar 42,5%, dari rata-rata skor awal 57,8 menjadi 82,4 setelah mengikuti kegiatan. Selain peningkatan pengetahuan, program ini juga menghasilkan daftar awal pendonor keluarga yang selanjutnya dikelola oleh tenaga kesehatan setempat. Antusiasme tinggi tercermin dari partisipasi aktif peserta dan permintaan lanjutan untuk pemeriksaan golongan darah bagi anggota keluarga lainnya. Temuan ini menunjukkan bahwa Program GEROBAK efektif dalam meningkatkan kesiapsiagaan desa terhadap kebutuhan darah dalam kondisi darurat. Ke depan, disarankan agar program ini dikembangkan lebih lanjut melalui pendampingan berkelanjutan, pelatihan kader kesehatan, serta replikasi di desa-desa lain melalui kolaborasi multisektor untuk mewujudkan sistem kesehatan berbasis komunitas yang tangguh dan berdaya. Kata kunci: Donor darah;Pendonor keluarga, Transfusi ABSTRACTThe availability of blood in emergency situations remains a serious challenge in many regions, particularly in rural villages far from blood transfusion facilities. The GEROBAK program (Gerakan Donor Bersama Keluarga) emerges as an innovative solution to empower families in order to establish a self-sufficient blood donation system by increasing awareness of the importance of blood donation. This public service activity was implemented in Prayungan through active collaboration between the team and the government. The program involved by 30 participants, consisting of family representatives. The implementation methodology comprised three stages: preparation and coordination, socialization and education, and monitoring and evaluation. Pre-test and post-test results demonstrated a significant (42.5%) increase in participants' knowledge, with average scores rising from 57.8 to 82.4 after intervention. Furthermore, the program also established a preliminary registry of family donors which will be managed by authorized healthcare officer. High enthusiasm was shown through active participation and participant requests for blood type screenings for additional family members. These findings indicate that the GEROBAK program effectively increase public readiness for emergency blood needs. It is recommended that the program could be expanded through sustained mentoring, training of health cadres, and replication in other villages via multisectoral collaboration to building a resilient, community-based healthcare system capable of addressing critical blood supply challenges. Keywords: Blood donation; Family donors; Transfusion
OPTIMALISASI PERAN REMAJA SEBAGAI DUTA PENGGERAK DONOR DARAH MELALUI PROGRAM TEMAN DONOR Nuraini, Fatia Rizki; Muflikhah, Nina Difla
Jurnal Abdi Insani Vol 11 No 2 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i2.1590

Abstract

The availability of blood supply remains a crucial issue. But, concerns and lack of public understanding regarding blood donation can pose constraints in achieving the required fulfillment. Teman Donor program was introduced with the aim of inspiring adolescents, particularly high school students in Bojonegoro, to voluntarily participate in blood donation activities. Within the framework of this program, 28 selected adolescents from 9 high schools were engaged as blood donor ambassadors. The process involved several stages: the formation of Teman Donor, socialization and education on blood donation using training modules, forming the official teams of Teman Donor, guidance by the community service team, dissemination of information by Teman Donor through school social networks, and intensive monitoring and evaluation to ensure the effectiveness of the program. Program evaluation using questionnaires indicated an increase in adolescents knowledge and decrease in the level of anxiety and fear towards the blood donation process. A significant proportion, 82.5%, demonstrated good understanding. Furthermore, the majority of participants expressed readiness to blood donation by 87.3%. Through these works, Suggested that participation in blood donation activities can be enhanced and ensuring an adequate supply of blood for medical purposes.
Co-Authors Anggita, Etri Sukma Astuti, Retno Puji Fatia Rizki Nuraini Nuraini, Fatia Rizki Qosiani, Elfa Jiril Siti Nurkasanah Wayan Tunas Artama Wayan Tunas Artama