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All Journal Indonesian Journal of Biotechnology Jurnal Analis Kesehatan Jurnal Ilmu dan Teknologi Kesehatan Jurnal Abdi Insani Community Empowerment Jurnal Cakrawala Promkes Jurnal Medika: Medika JURNAL ILMIAH KESEHATAN Jurnal Ilmu Kesehatan Masyarakat
Fatia Rizki Nuraini
Department Of Blood Bank Technology, Undergraduate Program, Stikes Rajekwesi Bojonegoro Jalan Raya Dander, Ngumpak Dalem, Dander, Bojonegoro, East Java 62171, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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PEMERIKSAAN GOLONGAN DARAH SISTEM ABO RHESUS PADA MAHASISWA STIKES RAJEKWESI BOJONEGORO Fatia Rizki Nuraini; Nina Difla Muflikhah; Siti Nurkasanah
Jurnal Abdi Insani Vol 9 No 2 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i2.566

Abstract

Blood Type is an important thing to every person to understand and can identify using a specific methods, blood typing. Blood typing refers to a specific pattern of reaction to testing antisera within a given system. Blood typing have several methods, the common method that usually used is Direct Slide Test. Over a period of time, our understanding on blood groups has evolved to encompass not only transfusion-related problems but also specific disease association with RBC surface antigens. This community service activity aimed to provide blood typing examination for students who unknown their blood type and strenghten the understanding about the important of Blood Type. This activity was held on December 20, 2021 in Stikes Rajekwesi Bojonegoro. This Community service applied an examination test to several students who still unknown their blood type. Examination of blood group used the A-B-O system and rhesus with the slide test method. The results of the blood group examination of 16 students showed that the highest results were blood group B as many as 6 people (37.5%) and the least blood group being O and AB with 3 people each (18.75). Rhesus examination showed that all students were rhesus positive (100%). Students who examinated during the programmes doesn’t have undestanding of the important of knowing their blood type. Students of Stikes Rajekwesi Bojonegoro get sufficient understanding about the important of knowing their blood type and the blood typing showed 37,5% of student are B type, 18,75% are O and AB type each, while 100% rhesus positive.
Improvement of blood donor knowledge in community at Sumbertlaseh, Bojonegoro Nina Difla Muflikhah; Fatia Rizki Nuraini; Eva Riantika Ratna Palupi; Titik Nuryanti; Retno Puji Astuti
Community Empowerment Vol 8 No 1 (2023)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.7784

Abstract

Blood services is one of the health support systems which play important role for fulfilling blood stock, blood production, and distribution. Fulfilment of blood stock become the main focus of blood support unit in Indonesia. During the Covid-19 pandemic, Indonesia Government applied community activities restrictions enforcement which social distancing became the major reason for decreasing donor quantity in Bojonegoro while blood supply was needed from other units. In case of increasing blood donor, socialization programme held at Sumbertlaseh Village. This programme accommodated by Student of diploma programme of blood bank technology, undergraduate programme of ners, dan Blood Donor Unit Bojonegoro. The programme share information about the important and advantage of blood donor using various media, such as brochure, leaflet, and presentation. The results of the activity show that 78.26% of the community has good knowledge about blood donor.
Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia Fatia Rizki Nuraini; Ratna Setyaningsih; Ari Susilowati
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.71150

Abstract

The endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disk diffusion assay. The minimum inhibitory concentration (MIC) was measured using the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage because of the extract. The antibacterial compounds were then detected using GC‐MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequencing Among the 13 isolates, the endophytic fungi identified as Botryosphaeria rhodina AK32 produced the antibacterial compounds that exhibited the highest activity and a broad spectrum. Moreover, they were capable against resistant bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) with an MIC value of 1.56% for all the test bacteria. The mechanism of action of AK32 ethyl acetate extract seemed to affect the condition of bacterial cell walls, causing morphological alteration such as shrinkage of the cell, warted cells, and hollow cells. Based on GC‐MS, the antibacterial compounds of AK32 ethyl acetate extract were di‐n‐octyl phthalate, benzyl alcohol, high‐oleic (CAS) safflower oil, benzene acetonitrile, and benzotriazole.
Donor Keluarga Terintegrasi: Strategi Ketahanan Stok Darah di Fasilitas Pelayanan Kesehatan Primer Fatia Rizki Nuraini; Nina Difla Muflikhah; Rizky Nur Aprilianti Amalia; Rio Candra Pratama
Jurnal Medika: Medika Vol. 5 No. 1 (2026)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/hkmvgn09

Abstract

Ketersediaan darah di Indonesia masih menjadi tantangan, khususnya pada fasilitas pelayanan kesehatan primer. Kondisi ini berdampak pada keterlambatan penanganan kasus kegawatdaruratan akibat terbatasnya stok darah. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memperkuat ketahanan stok darah di tingkat puskesmas melalui Program Donor Keluarga Terintegrasi berbasis komunitas di wilayah kerja Puskesmas Sumberrejo, Kabupaten Bojonegoro. Metode yang digunakan meliputi edukasi dan literasi donor darah, serta pemetaan dan pendataan pendonor potensial berbasis keluarga melalui pemeriksaan golongan darah. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan dan kesiapan masyarakat untuk berpartisipasi dalam sistem donor keluarga, yang ditunjukkan oleh peningkatan skor rata-rata pretest dari 53,5 menjadi 89,9 pada posttest. Selain itu, pemeriksaan golongan darah terhadap peserta yang belum mengetahui golongan darahnya berhasil membentuk basis data awal calon pendonor, dengan distribusi golongan darah terbanyak adalah O (40%) dan seluruhnya memiliki rhesus positif. Respon masyarakat, perangkat desa, dan kader kesehatan menunjukkan dukungan positif terhadap keberlanjutan program. Kegiatan ini membuktikan bahwa pendekatan berbasis komunitas yang terintegrasi dengan program puskesmas efektif dalam meningkatkan literasi, partisipasi, dan kesiapsiagaan donor darah, serta berpotensi memperkuat kemandirian sistem stok darah di tingkat pelayanan kesehatan primer.
A retrospective study of HBV reactivity among blood donors in Bojonegoro Fatia Rizki Nuraini; Nina Difla Muflikhah
Jurnal Cakrawala Promkes Vol. 8 No. 1 (2026): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jcp.v8i1.13840

Abstract

Hepatitis B virus (HBV) remains one of the most significant transfusion-transmitted infections (TTIs), posing ongoing challenges to blood safety in Indonesia. Regular monitoring of HBV reactivity among blood donors is crucial for evaluating the effectiveness of screening and guiding public health interventions. This study aimed to assess the prevalence and temporal trends of HBV reactivity among blood donors in Bojonegoro, while evaluating the effectiveness of current screening procedures and supporting public health interventions. A retrospective cross-sectional study was conducted on 51,351 blood donors at UDD PMI Bojonegoro between January 2020 and December 2021. Blood samples were screened using the Enzyme-Linked Immunosorbent Assay (ELISA) to detect HBV infection. Data were analyzed using descriptive statistics and cross-tabulation. A total of 261 donors tested reactive for hepatitis B, comprising 129 cases in 2020 and 132 in 2021. Although the absolute number of reactive cases increased slightly, the reactivity rate declined from 0.61% to 0.44%, coinciding with an overall increase in the number of donors. Monthly analysis revealed fluctuating patterns, with peaks observed in December 2020 and June 2021. The declining proportion of HBV reactivity suggests improvements in donor selection and public awareness. However, the continued presence of reactive cases highlights the need to strengthen screening systems, expand vaccination coverage, and adopt more sensitive detection technologies, such as Nucleic Acid Testing (NAT). Ongoing regional surveillance remains crucial to support national efforts in controlling HBV and ensuring the safety of blood transfusion services in Indonesia.
Kontaminasi Bakteri pada Alat Tensimeter di Ruang Penyadapan Darah Unit Donor Darah Bojonegoro Sherly Damara; Fatia Rizki Nuraini; Evita Muslima Isnanda Putri
Jurnal Analis Kesehatan Vol. 15 No. 1 (2026): JURNAL ANALIS KESEHATAN
Publisher : Medical Laboratory Technology Department, Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v15i1.5485

Abstract

Kontaminasi bakteri pada produk darah merupakan risiko yang harus dihindari, karena dapat terjadi pada setiap tahap pelayanan darah, mulai dari penusukan awal hingga distribusi. Salah satu sumber kontaminasi adalah alat medis, seperti tensimeter, yang digunakan untuk membendung lengan pendonor. Apabila tidak dibersihkan secara rutin, tensimeter dapat menjadi media pertumbuhan bakteri akibat penggunaan berulang. Penelitian ini bertujuan untuk mengidentifikasi pertumbuhan dan kelompok bakteri pada alat tensimeter berdasarkan pewarnaan Gram di ruang penyadapan darah UDD PMI Kabupaten Bojonegoro. Penelitian ini menggunakan desain deskriptif. Populasi alat tensimeter di ruang penyadapan darah UDD PMI Kabupaten Bojonegoro dengan sampel sebanyak 4 manset bagian dari tensimeter. Pengambilan sampel pada penelitian,  dengan melakukan swab pada bagian manset kemudian ditumbuhkan pada media Nutrient Agar dan bakteri yang tumbuh dilakukan pewarnaan Gram. Hasil penelitian didapatkan bahwa seluruh tensimeter (100%) yang diperiksa menunjukkan adanya pertumbuhan bakteri dengan bakteri Gram positif sebanyak 19 (79%) dan bakteri Gram negatif sebanyak 5 (21%). Sterilisasi dan desinfeksi pada alat medis yang digunakan oleh UDD PMI Kabupaten Bojonegoro harus diterapkan dan distandarkan melalui SOP sehingga akan menekan pertumbuhan bakteri pada alat medis yang digunakan.
IDENTIFIKASI TINGKAT KONTAMINASI BAKTERI PADA UDARA DI LABORATORIUM KOMPONEN DARAH UNIT DONOR DARAH (UDD) PMI KABUPATEN BOJONEGORO: IDENTIFICATION OF BACTERIAL CONTAMINATION LEVELS IN AIR AT THE BLOOD COMPONENT LABORATORY OF THE BLOOD DONOR UNIT (UDD) INDONESIAN RED CROSS (PMI) BOJONEGORO REGENCY Zohra Gretta Zalika; Wiwik Utami; Fatia Rizki Nuraini
Jurnal Ilmiah Kesehatan Vol. 7 No. 1 (2026): Jurnal Ilmiah Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajekwesi Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Kontaminasi bakteri pada komponen darah merupakan risiko serius dalam transfuse darah, dengan potensi infeksi lebih tinggi dari virus. Dampak kontaminasi udara bervariasi dari reaksi ringan seperti demam hingga berat sampai sepsis. Bakteri mudah beradaptasi di berbagai lingkungan, menjadi penyebab kematian nomor dua akibat transfusi. Tujuan penelitian mengidentifikasi kontaminasi bakteri pada udara berdasarkan jumlah koloni di laboratorium komponen darah Unit Donor Darah (UDD) PMI Kabupaten Bojonegoro. Penelitian menggunakan desain deskriptif. Populasi Seluruh mikro bakteri yang ada di laboratorium komponen darah Unit Donor Darah (UDD) PMI Kabupaten Bojonegoro, sampel mikro bakteri yang dibagi atas 5 titik dan setiap titik ada 5 cawan dengan teknik accidental sampling. Instrumen media Plate Count Agar (PCA) dan diinkubasi selama 1 x 24 jam dalam suhu 37 derajat celcius. Untuk melihat jumlah koloni bakteri di lima titik laboratorium komponen darah. Hasil penelitian didapatkan kontaminasi bakteri di Ruang Laboratorium Komponen Darah sebesar 1,479 CFU/m³. hal ini tidak melebihi ambang batas yang ditetapkan oleh Kepmenkes No. 1204/Menkes/SK/X/2004. UUD PMI disarankan memperkuat sanitasi dan sterilitas ruangan laboratorium komponen melalui penerapan SOP, dan investasi teknologi modern, pelatihan staf. Langkah proaktif ini penting untuk menjaga keamanan laboratorium dan kualitas produk darah.. Kata Kunci: Kontaminasi, Bakteri, Udara ABSTRACT Bacterial contamination of blood components is a serious risk in blood transfusions, with a higher potential for infection than viruses. The impact of air contamination varies from mild reactions such as fever to severe to sepsis. Bacteria are adaptable in various environments, being the number two cause of death due to transfusions. The purpose of the study was to identify bacterial contamination in the air based on the number of colonies in the blood component laboratory of the Blood Donor Unit (UDD) of PMI Bojo Regency. The research uses a descriptive design. Population All micro bacteria in the blood component laboratory of the Blood Donor Unit (UDD) PMI Bojonegoro Regency, microbacterial samples were divided into 5 points and each point had 5 cups with an accidental sampling technique. Plate Count Agar (PCA) media instrument and incubated for 1 x 24 hours at a temperature of 37 degrees Celsius. To see the number of bacterial colonies at five points of the blood component laboratory. The results of the study were obtained bacterial contamination in the Blood Component Laboratory Room of 1,479 CFU/m³. This does not exceed the threshold set by the Ministry of Health No. 1204/Menkes/SK/X/2004. The PMI Constitution recommends strengthening the sanitation and sterility of component laboratory rooms through the implementation of SOPs, and investment in modern technology, staff training. This proactive step is important to maintain laboratory safety and quality of blood products. Keywords: Contamination, Bacteria, Aira
Prevalence of Syphilis Cases Among Blood Donors in Bojonegoro Fatia Rizki Nuraini; Nina Difla Muflikhah
Jurnal Ilmu Kesehatan Masyarakat Vol. 13 No. 2 (2022): Jurnal Ilmu Kesehatan Masyarakat (JIKM)
Publisher : Faculty of Public Health, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26553/jikm.2022.13.2.145-154

Abstract

Syphilis is a disease caused by Treponema pallidum. Syphilis could transmit through sexual intercourse, and can also occur in the vertical direction from the mother to the fetus, through blood products and, in some cases, through medical equipment. Among those, blood transfusions are the main way to spread the disease. WHO estimates that more than 1 million people worldwide are diagnosed with 4,444 sexually transmitted diseases daily. This study was using total 51351 blood sample obtained from blood donor in 2020 and 2021 in Blood Donor Unit (UDD PMI) Bojonegoro. The aim in this study was to find the prevalence of syphilis cases occurred in Blood Donor Unit (UDD PMI) Bojonegoro. This finding may have implication to increase people awareness in syphilis. This study was used descriptive study with cross sectional design and conducted by using cross tabulation as statistical analysis. The data showed that there was slight increase with 0.12% and 0.16% cases of confirmed syphilis in 2020 and 2021 respectively (P = 0,174). Total cases from 51351 blood samples, 71 (0,14%) blood samples were positive for syphilis. The highest cases in 2020 was occurred in January (7 cases) and highest cases in 2021 was occurred in October (13 cases). The conclusion of this study showed that the prevalence of syphilis was low in this study. Even though the percentage might be below 1% in this study, it is still a significant transfusion problem in blood donation quality purpose to be overcomed.
A Comparative Study of Hematocrit Levels in Packed Red Cells Using Sedimentation and Centrifugation Method Saqif Bagas Baihaqi; Fatia Rizki Nuraini; Sri Mulyani
Jurnal Ilmu dan Teknologi Kesehatan Vol 14 No 1 (2026): September 2026
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v14i1.2718

Abstract

Packed Red Cells (PRC) are blood components that require appropriate processing to achieve hematocrit levels that meet quality standards. The separation method may influence the hematocrit (HCT) level of the final PRC product. This study aimed to determine and compare hematocrit levels in PRC products prepared using sedimentation and centrifugation at the Indonesian Red Cross (PMI) Blood Management Unit, Bojonegoro Regency. This quantitative comparative study included eight blood units selected by purposive sampling, with four units processed using sedimentation and four using centrifugation. Hematocrit levels were measured using the Mission HemoPro analyzer and compared using the Mann–Whitney U test. PRC prepared using sedimentation had hematocrit levels ranging from 52% to 63%, with a median of 56.0%, whereas PRC prepared using centrifugation had hematocrit levels ranging from 70% to 76%, with a median of 73.5%. Hematocrit levels differed significantly between the two groups (exact p=0.029), with higher values observed in the centrifugation group. In conclusion, PRC products prepared using centrifugation showed higher hematocrit levels than those prepared using sedimentation under the conditions of this study. Further studies with larger, statistically determined sample sizes are needed to confirm these findings.
Co-Authors ARI SUSILOWATI Eva Riantika Ratna Palupi Nina Difla Muflikhah Nina Difla Muflikhah Nina Difla Muflikhah Putri, Evita Muslima Isnanda RATNA SETYANINGSIH Retno Puji Astuti Rio Candra Pratama Rizky Nur Aprilianti Amalia Saqif Bagas Baihaqi Sherly Damara Siti Nurkasanah Sri Mulyani Titik Nuryanti Wiwik Utami Zohra Gretta Zalika