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All Journal The Indonesian Biomedical Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Theodora, Imelda
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Biochemistry, Genetics & Molecular Biology Health Professions Public Health Social Sciences

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Modified High-Fat High-Sucrose Diet Promotes Obesity and Alters Colonic Cytokines Novita, Bernadette Dian; Wedharga, I Gede Putu Adhi; Tjahjono, Yudy; Wijaya, Hendy; Theodora, Imelda; Ervina, Martha; Wilianto, Yufita Ratnasari; Dewi, Sianty; Parengkuan, Irene Lingkan; Herjunianto, Herjunianto; Ghasani, Sabrina Maria; Limantoro, Michael Christian; Jaya, Ferdinand Wiliam
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3807

Abstract

BACKGROUND: Western dietary patterns are often associated with increasing non-communicable diseases, including inflammatory bowel disease (IBD). In experimental models, a high-fat high-sucrose (HFHS) diet is used to mimic metabolic and inflammatory effects of such diets, however the data regarding colonic inflammation in Indonesia remain limited. Therefore, this study was conducted evaluated the impact of HFHS feeding on colonic interleukin (IL)-6, IL-10 expression, and the IL-6/IL-10 ratio.METHODS: Six weeks old male C57BL/6 mice were randomly assigned to a normal fat diet (NFD) or HFHS diet group and fed ad libitum for 8 weeks. Colonic tissues were collected, and IL-6 and IL-10 expression was analyzed by immunohistochemistry.RESULTS: HFHS-fed mice showed significant increases in body weight (increased by 22.44%, p=0.0047) and caloric intake (increased by 125.17%, p=0.0000), confirming obesity induction. Colitis was also evident, with higher histological colitis scores (p=0.0072). However, colonic IL-6 (increased by 9.12%, p=0.1236), IL-10 (increased by 1.49%, p=0.8013), and the IL-6/IL-10 ratio (increased by 7.38%, p=0.4000) showed no significant differences compared to NFD.CONCLUSION: In C57BL/6 mice, an 8-week modified HFHS diet induced obesity, increased caloric intake, and mucosal injury, but did not significantly alter colonic IL-6, IL-10, or their ratio. This suggests preserved mucosal immune homeostasis consistent with an early compensatory phase rather than overt cytokine-driven inflammation. Longer or more intensive exposure may disrupt this balance, highlighting the need for further studies to define the temporal threshold and clarify immune microbiome interactions in colitis progression.KEYWORDS: high-fat high-sucrose diet, colon inflammation, IL-6, IL-10, obesity mice
STAT3, but not Ki-67, expression is independently associated with tumor progression in colorectal carcinoma: A comparative immunohistochemical biomarker analysis Wedharga, I Gede Putu Adhi; Willianto, Vincentius Michael; Theodora, Imelda; Parengkuan, Irene; Novita, Bernadette Dian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.7711

Abstract

The insidious progression of colorectal carcinoma (CRC) at early stages often hampers timely screening and diagnosis, resulting in delayed intervention and poor clinical outcomes. Ki-67 and Signal Transducer and Activator of Transcription 3 (STAT3) have been widely studied for their roles in tumor proliferation and survival. However, comparative studies assessing their clinicopathological relevance in CRC progression remain limited, and their relative contributions to tumor aggressiveness are unclear. This study aimed to evaluate the clinicopathological significance and association of Ki-67 and STAT3 expression with tumor aggressiveness, focusing on their roles in tumor infiltration and size as indicators of CRC progression. Thirty-eight formalin-fixed, paraffin-embedded (FFPE) CRC tissue specimens were collected from patients undergoing surgical resection for this cross-sectional study. Ki-67 and STAT3 expression were assessed using immunohistochemical (IHC) staining. Clinicopathological data were retrieved from medical records. Protein expression was quantified using inverted mean gray values via digital image analysis. Ki-67 and STAT3 expression levels significantly correlated with tumor infiltration (Ki-67: r = 0.322, p = 0.049; STAT3: r = 0.429, p = 0.007) and size (Ki-67: r = 0.425, p = 0.008; STAT3: r = 0.452, p = 0.004). No significant correlation was found between Ki-67 and STAT3 (r = 0.225, p = 0.175). Only STAT3 was independently associated with infiltration (OR per 1-standard deviation: 4.11, p = 0.043) and size (OR per 1-SD: 3.41, p = 0.047). While both markers are associated with CRC progression, only STAT3 shows an independent association with tumor aggressiveness, underscoring its role beyond cell proliferation and suggesting broader involvement in tumor invasion and progression pathways.
Tumor Differentiation is Correlated with Estrogen Receptor Beta (ERβ) Expression but Not with Interleukin-6 (IL-6) Expression in Colorectal Carcinoma Theodora, Imelda; Sudiana, I Ketut; Budipramana, Vicky Sumarki; Erwin, Ferdinand; Dewi, Sianty; Novita, Bernadette Dian
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3324

Abstract

BACKGROUND: Colorectal carcinoma (CRC), the third most common malignant disease worldwide, is associated with estrogen receptor β (ERβ) and interleukin-6 (IL-6). ERβ is known to down-regulate IL-6 in prostate cancer, lung carcinoma, and CRC cell lines; however, its effect on human with CRC remains unclear. Therefore, this study was conducted to investigate the association between ERβ and IL-6 expressions with the clinicopathological features of CRC.METHODS: This was an analytic observational study using 40 paraffin blocks of CRC patients. ERβ and IL-6 expression was measured by immunohistochemistry (IHC) staining. The percentage of immunoreactive tumor cell per 1000 cells was manually recorded and tumor differentiation as well as tumor infiltration were determined. Tumor differentiation was graded according to the World Health Organization (WHO) 2010 criteria, while tumor infiltration was defined based on the American Joint Committee on Cancer (AJCC) 8th edition.RESULTS: Fifty percent of samples were well-differentiated CRC, and 57.5% samples were T3 infiltration tumors. IHC staining showed 35.5% of samples were positive for ERβ expression, while 70.86% were positive for IL-6 expression. There were negative correlation of ERβ expression with tumor differentiation (p=0.018; r=-0.371), but no correlation with tumor infiltration (p=0.836) were found. There was no correlation between ERβ expression with IL-6 expression (p=0.154).CONCLUSION: There is statistically significant correlation between tumor differentiation and ERβ expression, wherein improved tumor differentiation is linked to higher levels of positive ERβ expression. However, there is no discernible relationship between IL-6 and tumor differentiation. These findings suggest that while IL-6 was involved in the growth of the tumor, ERβ expression might have an impact on tumor differentiation.KEYWORDS: colorectal carcinoma, estrogen receptor beta, interleukin-6, cell differentiation
Co-Authors Budipramana, Vicky Sumarki Erwin, Ferdinand Ghasani, Sabrina Maria Herjunianto Herjunianto Irene Lingkan Parengkuan Jaya, Ferdinand Wiliam Limantoro, Michael Christian Martha Ervina Novita, Bernadette Dian Parengkuan, Irene S.Pd. M Kes I Ketut Sudiana . Sianty Dewi Tjahjono, Yudy Wedharga, I Gede Putu Adhi Wijaya, Hendy Wilianto, Yufita Ratnasari Willianto, Vincentius Michael