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All Journal Folia Medica Indonesiana Gadjah Mada Journal of Psychology The Indonesian Biomedical Journal Universa Medicina Jurnal Medik Veteriner Journal Of Widya Medika Junior INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Widya Medika

Novita, Bernadette Dian

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Author-ID : 1195943

Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Manajemen Laktasi dan Kesejahteraan Ibu Menyusui Wattimena, Inge; Werdani, Yesiana Dwi W.; Novita, Bernadette Dian; Dewi, D. A. Liona
Jurnal Psikologi Vol 42, No 3 (2015)
Publisher : Faculty of Psychology, Universitas Gadjah Mada

A motherâ€™s success of lactation depends on her self-management. This study aimed to reveal the contribution of self-awareness (introspection and observation) and self-determination (competency and autonomy) as self-management factors to a lactating motherâ€™s well-being (self-actualization and positive mood). The subjects of this study were 100 mothers who had been breastfeeding for at least six weeks. A structured model was designed with self-awareness as an independent variable to dependent variable of well-being, and as an antecedent variable to self-determination affecting the dependent variable of well-being. Likert scale instruments were used. The data obtained from the questionnaires were analyzed using Structural Equation Modelling program. The models fitting the requirements showed that self-awareness had direct and indirect effects through self-determination on well-being, with a total effect of 0.924. It could be concluded that the success of lactation achieved through the self-management has a strong influence on well-being.

COMPARISON OF THE EFFECTIVENESS OF ANTIMICOTIC EXTRACT OF ETHANOL TEMU KUNCI AND TEMULAWAK ON CANDIDA ALBICANS Ekaristy, Mergivia; Novita, Bernadette Dian; Angelina, Zenia
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 2 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i2.3186

Introduction: Candida albicans are normal flora bacteria on the skin and mucous membranesof humans. Candida albicans are said to be one of the causes of vaginal discharge or fluoralbus. Temu kunci (Boesenbergia pandurata (Roxb.) Schlect) and temulawak (Curcumaxanthorrhiza Roxb) are the ingredients of herbal medicines with the potential as anantifungal. Purpose: This study aims to explain the differences in the antifungal effect of the Temukunci (Boesenbergia pandurata (Roxb.) Schlect) and Temulawak (Curcuma xanthorrhizaRoxb) extracts and explain how the combination of Temu kunci (Boesenbergia pandurata(Roxb.) Schlect) and Temulawak (Curcuma xanthorrhiza Roxb) extracts has an antifungaleffect on Candida albicans by using the experimental study of post-test control group design. Method: This study uses the Kirby-Baurer method and then measures the inhibitory zone byusing calipers then comparing the inhibition zone of each extract. Results: The research results show no difference in the effectiveness of each extract, and noinhibition zone was found for each extract. Conclusion: This study concludes that the two extracts are not effective against C.albicans.

ANALYSIS OF INCREASED C-REACTIVE PROTEIN LEVELS IN BLOOD SERUM OF RATTUS NORVEGICUS WISTAR STRAIN DUE TO INTAKE OF POLYETHYLENE MICROPLASTICS Julian, Alvin; Suwasanti, Niluh; Sincihu, Yudhiakuari; Novita, Bernadette Dian; Samsudin, Kevin
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 6, No 2 (2024): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v6i2.5511

Background: The widespread use of plastic and poor management of plastic waste is an environmental problem and has an impact on human health. The most commonly found microplastic contaminant is polyethylene polymer. Ingested microplastic particles will undergo an endocytosis mechanism and be absorbed into the bloodstream. This then triggers an increase in reactive oxygen species (ROS) which induces oxidative stress and results in an inflammatory response. This study tries to analyze the effect of administering microplastics on increasing blood serum C-reactive protein as a biomarker and marker of the inflammatory response due to microplastics. Method: The experimental analytical research used 42 Rattus Norvegicus Wistar Strain animals, which were divided into 5 experimental groups and 1 control group. Quantitative data measurements/collection were carried out at two times (pre-post-test control group design) and analyzed by non-parametric comparison using the Friedman Test to see the increase in C-Reactive Protein (CRP) levels in the blood serum of Rattus norvegicus Wistar Strain before and after being given intake polyethylene microplastics. Result: In all groups X0-X5, The results of the comparative test using the Friedman test showed a significant value of P = 0.000 (P < 0.05), so it can be concluded that there is a difference in pre and post experimental CRP levels. Discussion: The increase in serum CRP levels from the control group to the X5 treatment group may be due to oxidative stress mechanisms, especially in hepatocyte cells, smooth muscle cells, macrophage cells, endothelial cells, lymphocyte cells and adipocyte cells which induce the production of CRP protein, especially native C-reactive protein (nCRP) and monomeric C-reactive protein (mCRP). The difference in serum CRP levels was significant (P = 0.000), where there was an increase in serum CRP levels post treatment (post experimental) from the lowest mean of 0.05 mg/L (pre experimental) to the highest of 0.98 mg/L on average, indicating that administration of microplastic intake at the dose studied had an impact on increasing serum CRP levels in response to an inflammatory reaction. The limitation of this research is that there are no journals that examine microplastics on serum CRP levels, resulting in a lack of research that can be used as a reference or comparative theory. Conclusion: Administration of Microplastic Polyethylene at the dose studied in the treatment group caused a significant increase in Rattus Norvegicus Wistar Strain Serum CRP levels.

MENINGKATKAN PERAN AKTIF REMAJA SMA DALAM PROMOSI PENCEGAHAN INFEKSI MENULAR SEKSUAL Novita, Bernadette Dian; Dewi, Sianty; Hertanto, Maria Patricia Dian; Young, Andre; Khudrati, Wilson Christianto; Meredith, Jennifer
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 6 (2023): Volume 4 Nomor 6 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i6.21338

Infeksi Menular Seksual (IMS) adalah salah satu penyakit menular yang sering ditemui pada kelompok remaja. Pengaruh internal dan lingkungan tidak didukung oleh pemahaman mengenai kesehatan reproduksi dan penyakit menular seksual, pengawasan dari orang tua serta budaya masyarakat yang menganggap informasi ini sebagai hal yang tabu sehingga remaja mencari informasi dari sumber yang tidak tepat. Fakultas Kedokteran Universitas Katolik Widya Mandala Surabaya bersama Komisi Remaja Keuskupan Surabaya merasa perlu diadakan penyuluhan untuk menghasilkan agen edukatif dari kelompok usia remaja yang dapat memberikan edukasi serta konseling terkait pencegahan serta bahaya dari IMS. Pelatihan ini bertujuan untuk meningkatkan kemampuan remaja melakukan promosi kesehatan terkait IMS, pentingnya vaksinasi Human Papilloma Virus (HPV) untuk mencegah IMS serta etika dalam menggunakan media sosial. Metode pelaksanaan kegiatan melalui tahap pengembangan modul pelatihan, tahap implementasi dan pelatihan, serta tahap monitoring dan evaluasi. Tahap implementasi dilaksanakan dengan melakukan pelatihan kepada remaja dalam bentuk seminar awam melalui Zoom sedangkan tahap monitoring dan evaluasi melalui lomba poster dengan menggunakan media sosial Instagram. Evaluasi menggunakan pre-test dan post-test, serta penilaian karya poster digital di media sosial Instagram oleh ahli dengan menggunakan rubrik penilaian. Melalui kegiatan abdimas ini, mitra dapat turut berperan aktif melalui pengetahuan yang telah diperoleh kepada masyarakat, khusunya remaja melalui media sosial dengan bahasa yang mudah dipahami. Saran untuk kegiatan berikutnya adalah dengan memperluas materi terkait IMS dan agar kegiatan ini dapat dilakukan secara rutin.

The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study Novita, Bernadette Dian; Sutandhio, Silvia
Folia Medica Indonesiana Vol. 55 No. 4 (2019): December
Publisher : Universitas Airlangga

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

Rapid isolation and characterization of Wharton's jelly-derived mesenchymal stem cells maintained in fresh-prepared human AB-serum Tjahjono, Yudy; Dewi, Sianty; Novita, Bernadette Dian; Wijaya, Hendy; Putra, Brilliant Dwi; Kuncorojakti, Suryo; Hendriati, Lucia; Jong, FX Himawan Haryanto; Widodo, Teguh; Malonda, Franklin Vincentius
Universa Medicina Vol. 44 No. 1 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.65-72

Background Mesenchymal stem cells (MSCs) are valued in regenerative medicine for their multipotency, proliferative capacity, and immunomodulatory properties. Wharton’s jelly-derived MSCs (WJ-MSCs) from the umbilical cord offer a non-invasive, promising source for clinical applications, because easy isolation, lack of ethical concerns, and the presence of both embryonic and adult stem cells have made them a valuable source for use in therapeutic applications and regenerative medicine. This study aimed to optimize WJ-MSC isolation and characterization methods. Methods Human umbilical cords from three healthy donors were collected post-cesarean under strict inclusion criteria. WJ-MSCs were isolated using the explant culture method, with cells adhering to T75 flasks pre-coated with 2% gelatin. Cultures were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% freshly prepared Human AB serum and monitored for 21 days. Flow cytometry (BD FACSAria) was performed at passages 1 and 5 to assess MSC markers CD105, CD73, CD90, and CD44, alongside the exclusion marker CD45. Results WJ-MSCs exhibited fibroblast-like morphology by passage 1 and showed robust proliferation. Flow cytometry revealed high CD44 expression (~60%) at passage 1, while CD105, CD73, and CD90 became prominent by passage 5. CD45 remained low, suggesting minimal hematopoietic contamination. Conclusion This study confirms the feasibility of isolating and expanding WJ-MSCs using DMEM with 10% human AB serum. While consistent cell growth was achieved, the 21-day culture period may require optimization for scalability, including serum concentration, substrate coatings, and oxygen levels. CPJ-MSCs may be preferable for applications demanding rapid expansion and early marker expression.

THE EFFECT OF PHARMACOLOGY INTERACTIVE DIGITAL MEDIA IN THE FIELD OF INTEGUMENTARY SYSTEM ON THE LEVEL OF UNDERSTANDING AND MOTIVATION OF MEDICAL STUDENTS OF WIDYA MANDALA CATHOLIC UNIVERSITY Purnama, Stephany Venesia; Novita, Bernadette Dian; Oenarta, Dave Gerald
JURNAL WIDYA MEDIKA Vol. 11 No. 1 (2025): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v11i1.7495

Technology plays an important role in education today. Conventional learning media, such as books, articles, journals, and other forms of literature, tend to make students less motivated to learn because they are often perceived as less interesting, monotonous, and boring. In contrast, technology-based learning makes the process more engaging and enjoyable. In medical education, many subjects are essential to understand, one of which is pharmacology. Pharmacology needs to be understood because knowledge in this field is used as the basis for selecting appropriate treatments for patients according to their clinical conditions. The aim of this study is to determine the effect of the use of pharmacology interactive digital media in the integumentary system on the level of understanding and motivation of medical students at the Faculty of Medicine, Widya Mandala Surabaya Catholic University. The number of samples in this study was 70 participants, with the results showing a p-value of 0.000. Therefore, it can be concluded that the H1 is accepted. The use of pharmacology interactive digital media in the integumentary system has a significant impact on the level of understanding and motivation of medical students at the Faculty of Medicine, Widya Mandala Surabaya Catholic University.

Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.

HYPOGLYCEMIC, ANTI-INFLAMMATORY EFFECT OF PORANG (AMORPHOPHALLUS ONCHOPYLLUS) ON ALLOXAN-INDUCED DIABETIC RATS Young, Andre; Novita, Bernadette Dian; Ricardo, Evan; Dewi, I.G.A.P.D; Amadeo, Jovan; Dewi, Sianty; Tahalele, Paul
JURNAL WIDYA MEDIKA Vol. 9 No. 1 (2023): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v9i1.4173

Introduction: Iles-iles / Porang is a tuber-producing plant that is commonly found in Indonesia. One of the most sought after content from Porang is Glucomannan. One of Porang’s health benefit is related to the effect of lowering blood glucose levels because it can prevent glucose absorption. These benefit is interesting to study because there has been no research linking the use of Porang to reduce inflammatory process in hyperglycmic conditions. Purpose: This study aims to analyze the anti-inflammatory, anti-oxidant and hypoglycemis effects of Glucomannan from Porang (Amorphophallus onchophyllus) extract in experimental animals. Method: The research design was a true experimental post-test only control group with random sampling to determine 5 white rats into the normal group, positive control group, negative control group and treatment 1, 2 and 3. The positive control group received Acarbose therapy thile the negative control group received carboxy-methyl-cellulose (CMC) therapy. This study used Porang extract (Amorphophallus onchophyllus) with concentrations of 200, 400 and 800 mg/Kg in hyperglycemic white rats that had been induced by Aloxan. The study was conducte for 50 days and then blood and serum samples were taken to assess the effect of hypoglycemia, anti-inflammatory and anti-oxidant using blood glucose, Malondialdehyde and C-reactiveprotein (CRP) measurement. Result and Discussion: The results showed no significant difference between the groups of rats receiving Porang extract and the positive and negative control groups. However, the MDA levels after 50 days of intervention between the negative control group and the therapy with doses of 200, 400 and 800 mg/Kg showed significant differences. Similarly found for blood glucose levels after intervention between negative control group and the 200 and 400 mg/Kg therapy group. This results may be caused by the type of Porang used, the form of the Porang and the concentration level of the Porang extract. Conclusion: Porang with the type of Amorphophallus onchophyllus can’t be used directly, but requires further processing to obtain the active substance Glucomannan.

Modified High-Fat High-Sucrose Diet Promotes Obesity and Alters Colonic Cytokines Novita, Bernadette Dian; Wedharga, I Gede Putu Adhi; Tjahjono, Yudy; Wijaya, Hendy; Theodora, Imelda; Ervina, Martha; Wilianto, Yufita Ratnasari; Dewi, Sianty; Parengkuan, Irene Lingkan; Herjunianto, Herjunianto; Ghasani, Sabrina Maria; Limantoro, Michael Christian; Jaya, Ferdinand Wiliam
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3807

BACKGROUND: Western dietary patterns are often associated with increasing non-communicable diseases, including inflammatory bowel disease (IBD). In experimental models, a high-fat high-sucrose (HFHS) diet is used to mimic metabolic and inflammatory effects of such diets, however the data regarding colonic inflammation in Indonesia remain limited. Therefore, this study was conducted evaluated the impact of HFHS feeding on colonic interleukin (IL)-6, IL-10 expression, and the IL-6/IL-10 ratio.METHODS: Six weeks old male C57BL/6 mice were randomly assigned to a normal fat diet (NFD) or HFHS diet group and fed ad libitum for 8 weeks. Colonic tissues were collected, and IL-6 and IL-10 expression was analyzed by immunohistochemistry.RESULTS: HFHS-fed mice showed significant increases in body weight (increased by 22.44%, p=0.0047) and caloric intake (increased by 125.17%, p=0.0000), confirming obesity induction. Colitis was also evident, with higher histological colitis scores (p=0.0072). However, colonic IL-6 (increased by 9.12%, p=0.1236), IL-10 (increased by 1.49%, p=0.8013), and the IL-6/IL-10 ratio (increased by 7.38%, p=0.4000) showed no significant differences compared to NFD.CONCLUSION: In C57BL/6 mice, an 8-week modified HFHS diet induced obesity, increased caloric intake, and mucosal injury, but did not significantly alter colonic IL-6, IL-10, or their ratio. This suggests preserved mucosal immune homeostasis consistent with an early compensatory phase rather than overt cytokine-driven inflammation. Longer or more intensive exposure may disrupt this balance, highlighting the need for further studies to define the temporal threshold and clarify immune microbiome interactions in colitis progression.KEYWORDS: high-fat high-sucrose diet, colon inflammation, IL-6, IL-10, obesity mice

Co-Authors Amadeo, Jovan Angelina, Zenia Budipramana, Vicky Sumarki Dewi, D. A. Liona Dewi, I.G.A.P.D Ekaristy, Mergivia Erwin, Ferdinand Ghasani, Sabrina Maria Hendrata, Adi Pramono Herjunianto Herjunianto Hertanto, Maria Patricia Dian Inge Wattimena Irene Lingkan Parengkuan Jaya, Ferdinand Wiliam Jong, FX Himawan Haryanto Julian, Alvin Khudrati, Wilson Christianto Kuncorojakti, Suryo Limantoro, Michael Christian Lucia Hendriati Malonda, Franklin Vincentius Martha Ervina Meredith, Jennifer Oenarta, Dave Gerald Parengkuan, Irene Paul L Tahalele Purnama, Stephany Venesia Putra, Brilliant Dwi Ricardo, Evan S.Pd. M Kes I Ketut Sudiana . Samsudin, Kevin Sianty Dewi Silvia Sutandhio Sincihu, Yudhiakuari Soediono, Endang Isbandiati Sumi WIjaya, Sumi Suwasanti, Niluh Teguh Widodo Theodora, Imelda Tjahjono, Yudy Wedharga, I Gede Putu Adhi Wijaya, Hendy Wilianto, Yufita Ratnasari Willianto, Vincentius Michael Yesiana Dwi W. Werdani, Yesiana Dwi W. Young, Andre

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