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All Journal Jurnal Medik Veteriner Jurnal Widya Medika
Wedharga, I Gede Putu Adhi
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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HOW PRO-INFLAMMATORY WESTERN DIETS AND ANTI-INFLAMMATORY DIETARY INTERVENTIONS INFLUENCE NONALCOHOLIC FATTY LIVER DISEASE (NAFLD) PROGRESSION: A NARRATIVE REVIEW OF NUTRITIONAL MECHANISMS OF ACTION Wedharga, I Gede Putu Adhi
JURNAL WIDYA MEDIKA Vol. 11 No. 2 (2025): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v11i2.7560

Abstract

Unhealthy diets, such as Western-type diets, are major contributors to metainflammation underlying the progression of nonalcoholic fatty liver disease (NAFLD). This narrative review analyzed 10 studies (2014–2024) from Google Scholar and PubMed to explore dietary roles in NAFLD progression. Western-type, hypercaloric, high-fructose, and HFHS diets worsen NAFLD by increasing inflammatory biomarkers and reducing oxidative stress. Conversely, dietary interventions, including the DASH diet, freshwater fish–based diets, and the Mediterranean diet, attenuate metainflammation and slow NAFLD progression. The significance of this topic lies in the global health burden of NAFLD and the increasing recognition of metainflammation as a key driver of its progression. While several pharmacological candidates have shown promise, dietary strategies remain essential in clinical practice due to their accessibility, safety, and preventive potential. This review underscores the clinical relevance of targeting metainflammation through specific dietary interventions and offers insights for both therapeutic guidance and public health policy.
Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

Abstract

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.
IMMUNOMODULATORY EFFECTS OF HUMAN ALLOGENEIC MESENCHYMAL STEM CELLS IN AGING-RELATED FRAILTY: A SYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMIZED CONTROLLED TRIALS Wedharga, I Gede Putu Adhi; Jaya, Ferdinand Wiliam
JURNAL WIDYA MEDIKA Vol. 12 No. 2 (2026): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v12i2.8384

Abstract

Aging-related frailty is associated with immune dysregulation, including chronic inflammation and immunosenescence. Human allogeneic mesenchymal stem cells (hMSCs) have been proposed as immunomodulatory therapies; however, the responsive immune domains remain unclear. This systematic review and meta-analysis explored immune and inflammatory biomarkers reported in randomized controlled trials (RCTs) of hMSC therapy for aging-related frailty. Major databases were searched through April 2026 for RCTs enrolling adults aged ≥60 years with clinically diagnosed frailty. Outcomes encompassed innate inflammation, adaptive immune activation and composition, immunosenescence-related measures, and alloimmune responses. Biomarkers reported by at least two trials were pooled using a random-effects model. Three RCTs (n = 196) met the eligibility criteria. hMSC therapy was associated with a significant reduction in tumor necrosis factor-α (TNF-α) at 6 months (mean difference [MD] −0.61, 95% CI −1.08 to −0.14), while interleukin (IL)-6, IL-8, interferon (IFN)-γ, and the CD4/CD8 ratio showed no consistent effects. Limited alloimmune data indicated no clinically meaningful immune sensitization. Overall, hMSC therapy may preferentially attenuate innate TNF-α–driven inflammation, while other immune effects remain exploratory.
Co-Authors Hendrata, Adi Pramono Jaya, Ferdinand Wiliam Kuncorojakti, Suryo Martha Ervina Novita, Bernadette Dian Paul L Tahalele Sianty Dewi Soediono, Endang Isbandiati Sumi WIjaya, Sumi Tjahjono, Yudy Wijaya, Hendy