Qodriyah, Nur Romadhona Lailatul
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IDENTIFIKASI MISKONSEPSI SISWA KELAS XI SMA NEGERI 4 MALANG PADA MATERI HIDROKARBON MENGGUNAKAN INSTRUMEN DIAGNOSTIK THREE TIER Qodriyah, Nur Romadhona Lailatul; Rokhim, Deni Ainur; Widarti, Hayuni Retno; Habiddin, Habiddin
Jurnal Inovasi Pendidikan Kimia Vol 14, No 2 (2020): July (2020)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chemistry is one of the sciences that is considered difficult for some students. The difficulties in chemistry because many concepts in chemistry are complex and abstract. Hydrocarbons are one of the chemistry topics with abstract concepts and are difficult and often cause misconceptions on students. If students experience misconceptions on one of the basic concepts, then the possibility of the emergence of misconceptions will be even greater on more complex concepts. Therefore, the researcher conducted a study to identifying misconceptions of grade XI students on the Hydrocarbon topic using a three-tier diagnostic test instrument. This study was quantitative descriptive research. The research implemented on 17 – 29 January 2020 the subjects were students of XI grade of MIPA 2 and MIPA 3 of SMAN 4 Malang. The research instrument used was in the form of a three-tier multiple-choice test and has been tested and proven to be valid and reliable. Misconceptions were identified on 7 concepts from the hydrocarbon topic, including: the concept of Hydrocarbon Compounds (22,1%), Carbon Atoms (23,6%), Carbon Atomic Types (22,9%), Structure and Nomenclature of Hydrocarbons Compound (24,8%), Physical and Chemical Properties of Hydrocarbon Compounds (38,7%), Isomers (45,1%), and Hydrocarbon Compound Reactions (31,4%). Ilmu kimia merupakan salah satu ilmu yang dianggap sulit bagi sebagian siswa pada umumnya. Sulitnya ilmu kimia dikarenakan konsep dalam ilmu kimia banyak yang bersifat kompleks dan abstrak. Hidrokarbon merupakan salah satu materi kimia dengan konsep yang abstrak dan dirasa sulit, serta sering kali menimbulkan miskonsepsi. Apabila dalam belajar siswa mengalami miskonsepsi pada salah satu konsep dasar, maka kemungkinan munculnya miskonsepsi akan semakin besar pada konsep yang lebih kompleks. Oleh sebab itu peneliti melakukan penelitian yang bertujuan untuk mengidentifikasi miskonsepsi siswa kelas XI pada materi Hidrokarbon menggunakan instrumen tes diagnostik three-tier. Metode penelitian yang digunakan adalah deskriptif kuantitatif. Penelitian dilaksanakan pada tanggal 17 - 29 Januari 2020, subyek penelitian yaitu siswa kelas XI MIPA 2 dan MIPA 3 SMAN 4 Malang. Instrumen penelitian yang digunakan berupa soal tes pilihan ganda three-tier dan telah diuji coba serta terbukti valid dan reliabel. Jawaban siswa kemudian dianalisis dan dikategorikan berdasarkan tingkatan pemahaman. Hasil menunjukkan bahwa terjadi miskonsepsi sebesar 29,8% (kategori rendah). Miskonsepsi teridentifikasi pada 7 konsep materi hidrokarbon, diantaranya: konsep Senyawa Hidrokarbon (22,1%), Kekhasan Atom Karbon (23,6%), Jenis Atom Karbon (22,9%), Struktur dan Tata Nama Senyawa Hidrokarbon (24,8%), Sifat Fisik dan Kimia Senyawa Hidrokarbon (38,7%), Isomer (45,1%), dan Reaksi Senyawa Hidrokarbon (31,4%).
Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS) Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.34316

Abstract

Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.
Isolation and Identification of Indigenous Polypropylene-Degrading Bacteria Isolated From Bestari Landfill of Probolinggo Yuliana, Shinta; Wijayanti, Chandra; Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Fullerene Vol 10 No 2 (2025): Fullerene Journal Of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v10i2.718

Abstract

The Bestari Landfill of Probolinggo City collects a variety of plastics waste, most of it already in a degraded state. It is estimated to be potential habitat for polypropylene-degrading bacteria. Previous NGS studies identified 2,400 species of bacteria, but did not reveal their physiological and functional characteristics. This study aims to identify PP-degrading bacteria using 16S rRNA analysis. Isolation is carried out by the Enrichment method on Mineral Salt Media (MSM) containing PP granules. The research stages included sampling, isolation, and screening of bacteria for the most effective degrading agents. Six isolates of polypropylene-degrading bacteria were discovered at the Bestari Landfill of Probolinggo City, including B1UM1, B1UM2, B1UM3, B2UM1, B2UM2, and B2UM3. After 15 days of incubation, potential isolates B1UM1, B1UM2, and B2UM1 showed the highest polymer reduction of 8.25%, 7.15%, and 6.25%. Gram staining results showed that isolate B1UM1 was coccus and a Gram-positive bacterium, while isolates B1UM2 and B2UM1 were basil and Gram-positive. Genotypic identification through 16S rRNA sequence analysis showed that isolate B1UM1 had 100.0% similarity with Staphylococcus haemolyticus, BIUM2 had a 100% similarity to Bacillus cereus, and B2UM1 had a 99% had a similarity to Bacillus sp.