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All Journal Jurnal Nukleus Peternakan
Nalley, Wilmientje M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

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PENGARUH WAKTU PRA PEMBEKUAN TERHADAP KUALITAS SEMEN BEKU DALAM PENGENCER SPERMAX (The Effect of Pre-Freezing Time on The Quality of Frozen Semen of Landrace Boars in Spermax Diluent) Dui, Theresia A. A. T.; Nalley, Wilmientje M; Lawa, Alvrado B; Telupere, Franky M S; Hine, Thomas M; Setyani, Ni M P
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22383

Abstract

This research aimed to investigate the effect of pre-freezing time on the quality of frozen Landrace boar semen. Semen was collected from four 2-3 year old Landrace boars. The semen was evaluated macroscopically and microscopically. Subsequently, good quality semen was diluted in a basic extender, then subjected to a holding time of 2 hours at 27-28°C. The semen was then centrifuged at 2000 rpm for 15 minutes. The spermatozoa pellet was re-diluted with a Spermax extender, packaged into 0.5 mL straws, and equilibrated for 2 hours at 3-5°C. After equilibration, the straws were frozen by placing them 10 cm above the surface of liquid nitrogen at ±-110°C for durations of 3, 6, 9, and 12 minutes. The straws were then immersed into liquid nitrogen at -196°C. Thawing was performed by immersing the straws into a water bath containing water at 37°C for 30 seconds, followed by evaluation of semen quality. The results showed that pre-freezing time had no significant effect (P>0.05) on semen quality after dilution and equilibration. However, it had a significant effect (P<0.01) on the recovery rate, motility, and viability of spermatozoa after thawing, with the best treatment being 9 minutes. It was concluded that a pre-freezing time of 9 minutes resulted in the highest quality of frozen Landrace boar semen compared to other treatments.
EVALUASI KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER SPERMAX MODIFIKASI DENGAN WAKTU PRE-FREEZING YANG BERBEDA (Assessment of Landrace Boar Frozen Semen Quality in Modified Spermax Diluent Under Varying Pre-freezing Durations) Kii, Mariana B.I.; Hine, Thomas M; Bette, Yustiany Y; Nalley, Wilmientje M
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22385

Abstract

Research was conducted to investigate the impact of different pre-freezing durations (PF) using a modified spermax (M) extender on the quality of frozen landrace boar semen. The study utilized fresh semen from four healthy Landrace boars aged 2-3 years. Fresh semen meeting SNI standards was diluted in SM extender and held at room temperature (27-28℃) for two hours. Subsequently, the semen was centrifuged at 2000 rpm for 10 minutes, the supernatant was discarded, and the sperm pellet was re-diluted with SM extender at a 1:1 ratio. The semen was then packaged in 0.5 mL straws, placed on a freezing rack, and allowed to equilibrate for two hours at 3-5℃. Following equilibration, the semen was re-evaluated, and the straws were frozen above liquid nitrogen at -110℃ with varying pre-freezing times of 5, 8, 11, and 14 minutes. After the pre-freezing period, the straws were plunged into liquid nitrogen for final freezing and then stored in liquid nitrogen containers at -196℃. The results indicated that the duration of pre-freezing time did not lead to statistically significant differences (P>0,05) in sperm motility, viability, abnormality, and recovery rate (RR). In conclusion, pre-freezing times ranging from 5 to 14 minutes had a similar impact on the quality of frozen landrace boar semen.
Co-Authors Bette, Yustiany Y Dui, Theresia A. A. T. Kii, Mariana B.I. Lawa, Alvrado B Setyani, Ni M P Telupere, Franky M S Thomas Mata Hine