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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Journal Of Vocational Health Studies Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Sugihartina, Ganthina
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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ELEVATED BLOOD LEAD LEVELS IN WOMEN OF REPRODUCTIVE AGE DUE TO LEADS EXPOSURE FROM CONTAMINATED WATER Nursidika, Perdina; Kurnia, Dedeh; Sugihartina, Ganthina
Journal of Vocational Health Studies Vol. 7 No. 3 (2024): March 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I3.2024.175-180

Abstract

Background: Water quality can decrease due to population growth, domestic waste handling, climate, and human activities. Most of the people in Cimahi use well water for drinking and eating. However, using well water with high lead levels for consumption can adversely affect on the lead levels in the women's blood of childbearing age. Purpose: This study investigates the relationship between lead levels in well water and the corresponding levels found in women's blood in the Leuwigajah area of Cimahi. Method: Research samples were collected from both well water and the women's blood of childbearing age (15 - 49 years) who consume well water for drinking and cooking. Subsequently, sample preparation was conducted, and the samples were analyzed using an atomic absorption spectrophotometer. The Spearman correlation test was employed to analyze the data and establish any potential correlations. Result: The lead content in water ranged from 0.055 ppm to 0.089 ppm, exceeding the requirements for lead in clean water. Meanwhile, lead levels in the blood ranged from 1.5 ug/dL to 78.2 ug/dL, with 6.7% falling within the normal category, 40% in the tolerable category, 30% in the excess category, and 23.3% in the high category. The statistical results show a significant relationship between lead levels in well water and the blood, with a correlation value of 37.9%. The correlation value indicates a weak positive relationship. Conclusion: There is a relationship between lead levels in well water and women's blood.
DIFFERENCES IN THE STABILITY OF CHOLINESTERASE ENZYME ACTIVITY OF SERUM AND HEPARIN PLASMA SAMPLES USING PHOTOMETRIC KINETIC METHOD Rohayati, Rohayati; Budiana, Lufthy Leidi; Indra, Asep Iin Nur; Sugihartina, Ganthina
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2777

Abstract

Kesalahan dalam pemeriksaan laboratorium dapat terjadi pada setiap fase proses pengujian, tetapi sebagian besar kesalahan terjadi pada fase pra analitik dengan kontribusi sebesar 70%. Pemeriksaan kolinesterase merupakan pemeriksaan pada keracunan pestisida untuk mendeteksi adanya gangguan kesehatan yang kronis bahkan mematikan. Penelitian ini bertujuan untuk mengetahui stabilitas aktivitas enzim kolinesterase pada sampel serum dan plasma heparin pada suhu ruang. Penelitian ini dilakukan di Laboratorium Kimia Klinik Teknologi Laboratorium Medis Poltekkes Bandung pada bulan Mei 2024. Penelitian ini bersifat quasy experimental dengan memberi perlakuan berupa penyimpanan terhadap sampel serum dan plasma heparin selama 6 dan 24 jam pada suhu ruang yang kemudian aktivitas enzim kolinesterase dibandingkan dengan aktivitas enzim kolinesterase pada serum dan plasma heparin yang segera diperiksa. Pemeriksaan dilakukan dengan metode kinetik fotometrik menggunakan alat fotometer dengan panjang gelombang 405 nm dan dilakukan replikasi sebanyak 3 kali, sehingga didapatkan 90 data yang kemudian diolah menggunakan SPSS dengan uji general linear model (GLM). Secara statistik pada hasil uji GLM pada sampel serum dan plasma heparin menunjukkan nilai p < 0.05 yang berarti terdapat perbedaan stabilitas antara serum dan plasma heparin. Namun, berdasarkan hasil analisa secara klinis nilai Total Error (TE%) dengan penundaan selama 6 jam dan 24 jam terhadap pemeriksaan segera pada sampel serum secara berturut-turut sebesar 1,68% dan 6,44%, sedangkan pada sampel plasma heparin sebesar 3,66% dan 6,95%. Pemeriksaan kolinesterase memiliki nilai TEa% sebesar 8,9%, sehingga baik sampel serum maupun plasma heparin memiliki nilai TE% < TEa%. Berdasarkan hasil tersebut tidak terdapat perbedaan secara klinis.
KAJIAN KADAR TIMBAL PADA RAMBUT PETUGAS OPERATOR STASIUN PENGISIAN BAHAN BAKAR UMUM DI KAWASAN INDUSTRI Wasdili, Fini Ainun Qolbi; Arisanti, Annisa Ulfa Septiana; Mahargyani, Wikan; Sugihartina, Ganthina
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19262

Abstract

Increasing population growth, industrialization and the use of motorized vehicles are the factors causing the increase in air pollution. One of the particles contained in the exhaust gas emissions produced is lead. This study was aimed at the operators of the Public Fuel Filling Station in the Industrial Area of West Bandung District who are vulnerable to lead exposure from various pollutant sources. The purpose of this study was to describe the highest and lowest levels of lead accumulated in the hair of gas station operators. The method used is descriptive method. The research sample was taken from respondents who have worked as gas station operators for >1 year. The sample used was 19 gas station operators. Data was collected by filling out a questionnaire followed by laboratory examination. The results showed that 5 people had lead levels exceeding the normal threshold and 14 others had normal lead levels with an average of 13.52 g/g. The highest lead content in hair was 110.75 g/g and the lowest level was -3.50 g/g. With a threshold value of lead levels in hair that is <12 mg/g. The head of the gas station is advised to apply regulations on the use of appropriate PPE for gas station operators to minimize lead exposure. For further research, it is recommended to monitor lead (Pb) levels with different specimens.   Keywords      : Lead, Hair, Gas Station, Industry, Motor Vehicle
Co-Authors Arisanti, Annisa Ulfa Septiana Budiana, Lufthy Leidi Indra, Asep Iin Nur KURNIA, DEDEH Mahargyani, Wikan Perdina Nursidika rohayati rohayati Wasdili, Fini Ainun Qolbi