Article Per Year (5 Year)
p-Index From 2020 - 2025
1.126
P-Index
This Author published in this journals
All Journal Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan) Jurnal Riset Kesehatan Poltekkes Depkes Bandung Nutriture Journal Current Biomedicine Media Penelitian dan Pengembangan Kesehatan
Indra, Asep Iin Nur
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
PELATIHAN MEDIA PEMBELAJARAN KIMIA MENGGUNAKAN SOFTWARE KINGDRAW DAN PENGENALAN REAGEN ALAMI UNTUK ANALISIS DI SMK BHAKTI KENCANA rismiarti, zuri; Indra, Asep Iin Nur; Rohayati, Rohayati
Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan) Vol. 8 No. 1 (2024): Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan)
Publisher : Politeknik Kesehatan Kemenkes malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31290/j.idaman.v8i1.4201

Abstract

Organic chemistry learning materials about writing the structure of organic chemistry molecules, pictures of simple practical tools are obstacles in learning chemistry. This is due to the lack of technology-based skills to make it easier to write this material in teaching materials. One of the superior teaching methods that needs to be taken into consideration is the provision of multimedia-based teaching materials and supporting facilities. It is hoped that the availability of technological facilities and multimedia teaching materials will make it easier for students to understand the material/lessons. Strengthening the ability to master multimedia-based teaching materials is still hampered by the lack of skills in making KingDraw software teaching materials. Apart from that, the partner has 3 chemical laboratories but does not have a comprehensive insight into natural reagents for chemical analysis that can be used for chemistry practicum. Therefore, in this community service program scheme, the proposer provides several solutions and programs to provide development training. learning media/teaching materials through KingDraw software and introduction to natural reagents for chemical analysis at SMK Bhakti Kencana Cimahi. Strengthening students' competencies by applying the application of natural ingredients as reagents for chemical analysis can help with laboratory practicums in chemical analysis subjects. Thus, this community service activity can be useful for schools to improve the quality of teachers in updating knowledge based on KingDraw software technology to develop teaching materials.
Optimasi suhu amplifikasi DNA pada quantitative polymerase chain reaction untuk identifikasi Mycobcterium tuberculosis resistan isoniazid Endarwati, Dwi Veni; Indra, Asep Iin Nur; Hardiana, Acep Tantan; Abror, Yogi Khoirul; Nurhayati, Betty; Merdekawati, Fusvita
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.61-70

Abstract

Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.
Konsentrasi dan kemurnian ekstraksi DNA metode sonikasi dan spin column dari sampel dahak penderita tuberkulosis Saputra, Fitrianingsih; Indra, Asep Iin Nur; Djuminar, Ai; Merdekawati, Fusvita; Nurhayati, Betty
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.84-92

Abstract

Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.
indonesia VARIATION OF CONCENTRATION AND REPEATED USE OF TRIS ACETAT EDTA BUFFER ON DNA TAPE QUALITY Mycobacterium tuberculosis Makbul Paneja, Rosmini Binti; Rismiarti, Zuri; Indra, Asep Iin Nur; Wahyuni, Yeni
NUTRITURE JOURNAL Vol. 3 No. 2 (2024): Nutriture Journal
Publisher : Politeknik Kesehatan Kemenkes Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31290/nj.v3i2.4332

Abstract

Background: Mycobacterium tuberculosis which is resistant to isoniazid (INH) occurs due to gene mutations, mostly in the katG gene. Primers have been found to identify mutations in the katG gene that occur with varying lengths of primer products. However, this has not been proven laboratory-wise. Varying TAE buffer concentrations of 0.5x, 1x, and 1.5x were carried out, and repeated use of TAE buffer. This is to see the effect of concentration variations on the quality of the Mycobacterium tuberculosis DNA band. Method: The type of research used was quasi-experimental. The research subjects used were TAE buffer solution with concentrations of 0.5x, 1x and 1.5x which were used repeatedly at the electrophoresis stage. The examination material was Mycobacterium tuberculosis DNA resulting from PCR. Visualization of electrophoresis results on all samples compared to the size of the ladder used and all products produced are at the appropriate base pair size. Using the ImageJ application in the DNA size analysis process is an alternative that can help assess the size of DNA bands. Research Results: In the results of the research carried out, it was found that the DNA band of Mycobacterium tuberculosis which is resistant to the isoniazid (INH) katG gene for MDR TB is at codon 315 with a product size of 101 bp S315T, 141 bp S315N, 200 bp S315I, 247 bp S315R, 290 bp S315G and 400 bp R463L obtained DNA band density on electrophoresis using a TAE buffer concentration of 0.5x, shown by the highest area formed compared to concentrations of 1x and 1.5x, and from repeated use of TAE buffer twice, visualization of the DNA bands was obtained. still good, it just affects the area of ??the DNA band when read using the ImageJ application. Conclusion: 0.5x TAE buffer concentration is able to provide the highest area compared to 1x and 1.5x TAE buffer concentrations. The repetition process does not affect the visualization of the DNA bands but does affect the smaller area formed. Key words: Electrophoresis; TAE buffer concentration; Mycobacterium tuberculosis; Repeated use of TAE buffer
In silico prediction of multi-epitope vaccine candidates against Mycobacterium leprae Shabrina, Almas; Indra, Asep Iin Nur; Rinaldi, Sonny Feisal; Merdekawati, Fusvita
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.22

Abstract

Background Leprosy, also known as Hansen's disease, is an infectious disease caused by Mycobacterium leprae. Despite ongoing efforts to control the disease, leprosy remains a global health concern, with Indonesia ranking third in the world for the highest number of cases. Objective This study aims to identify epitopes that can induce T and B cell immune responses through an in silico approach, to design a multi-epitope vaccine candidate against Mycobacterium leprae. Methods The study used an in silico vaccine design approach utilizing ESAT6, Ag85B, ML2028, ML2380, and ML2055 proteins from Mycobacterium leprae. The process involved sequence alignment, T cell (CTL and HTL) and B cell epitopes identification, and antigenicity, allergenicity, and toxicity assessment. Selected epitopes were constructed into a multi-epitope vaccine candidate using linkers. The tertiary structure of the vaccine was modeled with AlphaFold and evaluated via Prosa-web. The stability and interaction between the vaccine candidate and TLR4 were analyzed using molecular docking. Results The vaccine candidate demonstrated stable interactions with TLR4, with a binding free energy of -13.9 kcal/mol. The vaccine candidate was also predicted to be stable, antigenic, non-allergenic, non-toxic, and hydrophilic. Conclusion This in silico design of a multi-epitope vaccine candidate shows potential for development as a vaccine against leprosy.
DIFFERENCES IN THE STABILITY OF CHOLINESTERASE ENZYME ACTIVITY OF SERUM AND HEPARIN PLASMA SAMPLES USING PHOTOMETRIC KINETIC METHOD Rohayati, Rohayati; Budiana, Lufthy Leidi; Indra, Asep Iin Nur; Sugihartina, Ganthina
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2777

Abstract

Kesalahan dalam pemeriksaan laboratorium dapat terjadi pada setiap fase proses pengujian, tetapi sebagian besar kesalahan terjadi pada fase pra analitik dengan kontribusi sebesar 70%. Pemeriksaan kolinesterase merupakan pemeriksaan pada keracunan pestisida untuk mendeteksi adanya gangguan kesehatan yang kronis bahkan mematikan. Penelitian ini bertujuan untuk mengetahui stabilitas aktivitas enzim kolinesterase pada sampel serum dan plasma heparin pada suhu ruang. Penelitian ini dilakukan di Laboratorium Kimia Klinik Teknologi Laboratorium Medis Poltekkes Bandung pada bulan Mei 2024. Penelitian ini bersifat quasy experimental dengan memberi perlakuan berupa penyimpanan terhadap sampel serum dan plasma heparin selama 6 dan 24 jam pada suhu ruang yang kemudian aktivitas enzim kolinesterase dibandingkan dengan aktivitas enzim kolinesterase pada serum dan plasma heparin yang segera diperiksa. Pemeriksaan dilakukan dengan metode kinetik fotometrik menggunakan alat fotometer dengan panjang gelombang 405 nm dan dilakukan replikasi sebanyak 3 kali, sehingga didapatkan 90 data yang kemudian diolah menggunakan SPSS dengan uji general linear model (GLM). Secara statistik pada hasil uji GLM pada sampel serum dan plasma heparin menunjukkan nilai p < 0.05 yang berarti terdapat perbedaan stabilitas antara serum dan plasma heparin. Namun, berdasarkan hasil analisa secara klinis nilai Total Error (TE%) dengan penundaan selama 6 jam dan 24 jam terhadap pemeriksaan segera pada sampel serum secara berturut-turut sebesar 1,68% dan 6,44%, sedangkan pada sampel plasma heparin sebesar 3,66% dan 6,95%. Pemeriksaan kolinesterase memiliki nilai TEa% sebesar 8,9%, sehingga baik sampel serum maupun plasma heparin memiliki nilai TE% < TEa%. Berdasarkan hasil tersebut tidak terdapat perbedaan secara klinis.
DIAGNOSTIK PAPER ANALYTICAL DEVICE (PAD) MENGGUNAKAN EKSTRAK UBI JALAR UNGU UNTUK DETEKSI BORAKS DALAM MAKANAN Rismiarti, Zuri; Indra, Asep Iin Nur; Agustini, Siti Fajarwati
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 3 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i3.1977

Abstract

Consuming foods containing borax (tetraborate) will cause health problems. However, in reality, borax is often added to foods such as crackers, meatballs, wet noodles, dumplings. These foods are usually produced by unregistered public members, so their quality is less controlled, so routine monitoring of borax levels in food is needed so that the food quality circulating in the community is maintained and safe for consumption. This research aimed to develop simple method that does not use special instruments to diagnose borax using a Paper Analytical Device (PAD) based on digital imaging using natural reagents from purple sweet potato extract. The research design was laboratory research and development (RnD). The research was conducted at Poltekkes Kemenkes Bandung in January-June 2022. The samples used were borax samples at concentrations of 100, 300, and 500 ppm. The research method was PAD based on digital imaging using Image J. This research was carried out by extracting anthocyanins from purple sweet potatoes, then optimizing the anthocyanin concentration extract; pH; measurement time, determining measurement linearity, and validation by determining % recovery. The research results include that the optimum pH is 9. Optimized measurement time is 3 minutes and is stable until the 5th minute, the linear concentration range of tetraborate is 100-500 ppm tetraborate. In the validation test of the PAD method, a recovery percentage of 87.58-87.95% was obtained for 200 and 300 ppm tetraborate samples. The research result will be able to diagnose and monitor borax in food.
Co-Authors Abror, Yogi Khoirul Agustini, Siti Fajarwati Budiana, Lufthy Leidi Djuminar, Ai Endarwati, Dwi Veni Hardiana, Acep Tantan Makbul Paneja, Rosmini Binti Merdekawati, Fusvita Nurhayati, Betty Rinaldi, Sonny Feisal rohayati rohayati Saputra, Fitrianingsih Shabrina, Almas Sugihartina, Ganthina Yeni Wahyuni Zuri Rismiarti