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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
KOSTAMAN, T.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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Conditioned media and DMSO enhance the cryopreservation of bovine adipose tissue-derived mesenchymal stem cells Irfan, S.; Suyatno, S.; Zulfiqar, H.; Lestari, D. A.; Hafid, A.; Kostaman, T.; Herdis, H.; Priyatno, T. P.; Sitaresmi, P. I.; Hudaya, M. F.; Lupitasari, F. B. I.; Pangestu, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 2 (2024): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.2.181-190

Abstract

Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de veloping countries. So, MSCs must be cryopreserved with an efficient cryoprotective agent to be stored and reproduced in various laboratories after isolation. This study was aimed to optimize the cryopreser vation media for adipose-derived MSCs in cattle. This study evaluated the viability, proliferation, and morphology of AD-MSCs. The results of this study indicate that a combination of 10% DMSO, 45% DMEM, and 45% conditioned media significantly improves post-thaw viability, proliferation, and sur vival as compared to other mediums. Furthermore, AD-MSCs cryopreserved in this medium exhibit similar morphology as fresh cells. These findings suggest that the optimized cryopreservation medium can enhance the quality and safety of AD-MSCs for clinical applications in the livestock industry.
The Resilience of Smallholder Layer-Chicken Farmers Throughout the COVID-19 Pandemic: Evidence from Indonesia Priyono; Kusumaningrum, D. A.; Rusdiana, S.; Praharani, L.; Widiawati, Y.; Kostaman, T.; Herliatika, A.; Pratiwi, N.; Azizah, N.; Iji, P. A.
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.392

Abstract

Smallholder layer-chicken farming in Indonesia is essential to ensure national food production, food security, and economic resilience. During the COVID-19 pandemic, small-scale layer-chicken farming exhibited adaptability. This study aims to assess the smallholder layer-chicken farmers resilience in Indonesia during the challenges posed by the COVID-19 pandemic. This research employed cross-sectional data collected from February to July 2023 from 114 smallholder layer chicken farmers in West Java, Central Java, and East Java using a purposive sampling technique. Data analysis used descriptive statistics, financial analysis, and the Wilcoxon Signed Rank Test. The results showed that the COVID-19 pandemic impacted the decline in the population and production of layer-chicken at the beginning of the COVID-19 pandemic but subsequently demonstrated an increase afterward. Financial analyses showed an increase in operational costs, specifically feed and labor, during the COVID-19 pandemic. The layer-chicken farms could adapt and maintain profitability amidst increasing operational costs. The findings showed that the COVID-19 pandemic significantly impacted increasing feed costs (p<0.05) and labor costs (p<0.05). The revenue-cost ratio remained relatively stable. The study also showed significant differences in profit levels before, during, and after the COVID-19 pandemic (p<0.05). Profit levels decreased during the beginning of the COVID-19 pandemic and recovered afterward, showcasing the smallholder layer chicken farmer’s resilience during the COVID-19 pandemic. These findings offer valuable insights for policy formulation aimed at fortifying the resilience demonstrated by smallholder layer-chicken farming in Indonesia amidst the challenges posed by the COVID-19 pandemic and other potential crises in the future.
Co-Authors A. Hafid, A. Azizah, N. Herdis, H. Herliatika, A. Hudaya, M. F. Iji, P. A. Irfan, S. Kusumaningrum, D. A. L. Praharani Lestari, D. A. Lupitasari, F. B. I. M. Pangestu N. Pratiwi, N. Priyono S. Rusdiana Sitaresmi, P. I. Suyatno, S. T. P. Priyatno, T. P. Y. Widiawati Zulfiqar, H.